(Linear) (Six-base) MAPSORT of: KIEE2075.seq Check: 4833 from: 1 to: 2217 KIEE2075.orf With 182 enzymes: SgfI * July 15, 2010 18:54 .. AccI GT'mk_AC Cuts at: 0 425 656 750 2217 Size: 425 231 94 1467 Fragments arranged by size: 1467 425 231 94 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1924 2217 Size: 1924 293 AflII C'TTAA_G Cuts at: 0 1321 2217 Size: 1321 896 AflIII A'CryG_T Cuts at: 0 1000 1870 2217 Size: 1000 870 347 Fragments arranged by size: 1000 870 347 AhdI GACnn_n'nnGTC Cuts at: 0 510 2217 Size: 510 1707 AlwNI CAG_nnn'CTG Cuts at: 0 418 2217 Size: 418 1799 ApaLI G'TGCA_C Cuts at: 0 282 1146 2217 Size: 282 864 1071 Fragments arranged by size: 1071 864 282 ApoI r'AATT_y Cuts at: 0 487 860 1477 2217 Size: 487 373 617 740 Fragments arranged by size: 740 617 487 373 AseI AT'TA_AT Cuts at: 0 916 1780 2217 Size: 916 864 437 Fragments arranged by size: 916 864 437 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1529 1562 2217 Size: 1529 33 655 Fragments arranged by size: 1529 655 33 BamHI G'GATC_C Cuts at: 0 351 2217 Size: 351 1866 BanI G'GyrC_C Cuts at: 0 1951 2217 Size: 1951 266 BanII G_rGCy'C Cuts at: 0 1114 1199 1258 2217 Size: 1114 85 59 959 Fragments arranged by size: 1114 959 85 59 BbeI G_GCGC'C Cuts at: 0 1955 2217 Size: 1955 262 BciVI GTATCCnnnnn_n' Cuts at: 0 1356 2217 Size: 1356 861 BglII A'GATC_T Cuts at: 0 332 947 2217 Size: 332 615 1270 Fragments arranged by size: 1270 615 332 Bme1580I G_kGCm'C Cuts at: 0 286 623 1150 2217 Size: 286 337 527 1067 Fragments arranged by size: 1067 527 337 286 BmrI ACTGGGnnnn_n' Cuts at: 0 1968 2217 Size: 1968 249 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 354 392 487 1218 2217 Size: 354 38 95 731 999 Fragments arranged by size: 999 731 354 95 38 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 400 463 514 743 792 1059 1327 1360 Size: 400 63 51 229 49 267 268 33 Cuts at: 1360 1591 2217 Size: 231 626 Fragments arranged by size: 626 400 268 267 231 229 63 51 49 33 Bpu10I CC'TnA_GC Cuts at: 0 1108 2090 2217 Size: 1108 982 127 Fragments arranged by size: 1108 982 127 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 50 1623 2217 Size: 50 1573 594 Fragments arranged by size: 1573 594 50 BsaI GGTCTCn'nnnn_ Cuts at: 0 76 2217 Size: 76 2141 BsaHI Gr'CG_yC Cuts at: 0 1952 2217 Size: 1952 265 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 531 561 974 1004 1838 1868 1916 1946 Size: 531 30 413 30 834 30 48 30 Cuts at: 1946 2217 Size: 271 Fragments arranged by size: 834 531 413 271 48 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1092 2217 Size: 1092 1125 BseYI C'CCAG_C Cuts at: 0 385 2217 Size: 385 1832 BsiHKAI G_wGCw'C Cuts at: 0 286 1150 1199 1258 2105 2217 Size: 286 864 49 59 847 112 Fragments arranged by size: 864 847 286 112 59 49 BsmI GAATG_Cn' Cuts at: 0 1686 2217 Size: 1686 531 BsmBI CGTCTCn'nnnn_ Cuts at: 0 132 2217 Size: 132 2085 Bsp1286I G_dGCh'C Cuts at: 0 286 623 1114 1150 1199 1258 2105 2217 Size: 286 337 491 36 49 59 847 112 Fragments arranged by size: 847 491 337 286 112 59 49 36 BspHI T'CATG_A Cuts at: 0 473 2217 Size: 473 1744 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2139 2217 Size: 2139 78 BsrBI CCG'CTC Cuts at: 0 1067 2217 Size: 1067 1150 BsrFI r'CCGG_y Cuts at: 0 1301 2217 Size: 1301 916 BsrGI T'GTAC_A Cuts at: 0 1867 2217 Size: 1867 350 BssSI C'ACGA_G Cuts at: 0 1243 2098 2217 Size: 1243 855 119 Fragments arranged by size: 1243 855 119 BstBI TT'CG_AA Cuts at: 0 1481 2217 Size: 1481 736 BstXI CCAn_nnnn'nTGG Cuts at: 0 531 1892 2217 Size: 531 1361 325 Fragments arranged by size: 1361 531 325 BstYI r'GATC_y Cuts at: 0 332 351 947 1215 2217 Size: 332 19 596 268 1002 Fragments arranged by size: 1002 596 332 268 19 BstZ17I GTA'TAC Cuts at: 0 426 2217 Size: 426 1791 Bsu36I CC'TnA_GG Cuts at: 0 347 2217 Size: 347 1870 DraI TTT'AAA Cuts at: 0 75 2217 Size: 75 2142 EarI CTCTTCn'nnn_ Cuts at: 0 472 2055 2217 Size: 472 1583 162 Fragments arranged by size: 1583 472 162 EcoICRI GAG'CTC Cuts at: 0 1197 1256 2217 Size: 1197 59 961 Fragments arranged by size: 1197 961 59 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 400 463 514 743 792 1059 1327 1360 Size: 400 63 51 229 49 267 268 33 Cuts at: 1360 1591 1924 2217 Size: 231 333 293 Fragments arranged by size: 400 333 293 268 267 231 229 63 51 49 33 EcoRI G'AATT_C Cuts at: 0 1477 2217 Size: 1477 740 EcoRV GAT'ATC Cuts at: 0 103 2217 Size: 103 2114 HaeII r_GCGC'y Cuts at: 0 1955 2217 Size: 1955 262 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 112 144 177 209 231 263 496 528 Size: 112 32 33 32 22 32 233 32 Cuts at: 528 957 974 989 1006 1360 1392 1838 1870 Size: 429 17 15 17 354 32 446 32 Cuts at: 1870 2217 Size: 347 Fragments arranged by size: 446 429 354 347 233 112 33 32 32 32 32 32 32 22 17 17 15 HincII GTy'rAC Cuts at: 0 998 2217 Size: 998 1219 HindIII A'AGCT_T Cuts at: 0 68 2217 Size: 68 2149 KasI G'GCGC_C Cuts at: 0 1951 2217 Size: 1951 266 MslI CAynn'nnrTG Cuts at: 0 368 602 1466 2140 2217 Size: 368 234 864 674 77 Fragments arranged by size: 864 674 368 234 77 NarI GG'CG_CC Cuts at: 0 1952 2217 Size: 1952 265 NspI r_CATG'y Cuts at: 0 1004 1601 1874 2217 Size: 1004 597 273 343 Fragments arranged by size: 1004 597 343 273 PciI A'CATG_T Cuts at: 0 1000 1870 2217 Size: 1000 870 347 Fragments arranged by size: 1000 870 347 PflMI CCAn_nnn'nTGG Cuts at: 0 202 2217 Size: 202 2015 PfoI T'CCnGG_A Cuts at: 0 1036 1568 2217 Size: 1036 532 649 Fragments arranged by size: 1036 649 532 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 256 288 1120 1152 2217 Size: 256 32 832 32 1065 Fragments arranged by size: 1065 832 256 32 32 SacI G_AGCT'C Cuts at: 0 1199 1258 2217 Size: 1199 59 959 Fragments arranged by size: 1199 959 59 ScaI AGT'ACT Cuts at: 0 1934 2217 Size: 1934 283 SfcI C'TryA_G Cuts at: 0 799 1663 1943 2217 Size: 799 864 280 274 Fragments arranged by size: 864 799 280 274 SfoI GGC'GCC Cuts at: 0 1953 2217 Size: 1953 264 SmlI C'TyrA_G Cuts at: 0 65 1321 1602 2217 Size: 65 1256 281 615 Fragments arranged by size: 1256 615 281 65 StyI C'CwwG_G Cuts at: 0 24 2217 Size: 24 2193 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 681 2217 Size: 681 1536 TatI w'GTAC_w Cuts at: 0 1867 1932 2217 Size: 1867 65 285 Fragments arranged by size: 1867 285 65 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 311 343 851 883 1175 1207 2217 Size: 311 32 508 32 292 32 1010 Fragments arranged by size: 1010 508 311 292 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 124 988 1852 2217 Size: 124 864 864 365 Fragments arranged by size: 864 864 365 124 XbaI T'CTAG_A Cuts at: 0 97 1380 2217 Size: 97 1283 837 Fragments arranged by size: 1283 837 97 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 170 767 1034 2217 Size: 170 597 267 1183 Fragments arranged by size: 1183 597 267 170 Enzymes that do cut: AccI AcuI AflII AflIII AhdI AlwNI ApaLI ApoI AseI BaeI BamHI BanI BanII BbeI BciVI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BssSI BstBI BstXI BstYI BstZ17I Bsu36I DraI EarI EcoICRI Eco57MI EcoRI EcoRV HaeII Hin4I HincII HindIII KasI MslI NarI NspI PciI PflMI PfoI PpiI SacI ScaI SfcI SfoI SmlI StyI TaqII TatI TstI Tth111I XbaI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AgeI AleI AlfI AloI ApaI AscI AsiSI AvaI AvrII BbsI BbvCI BcgI BclI BfrBI BglI BlpI BmgBI BmtI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BspEI BsrDI BssHII BstAPI BstEII BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EagI EciI EcoNI EcoO109I FalI FseI FspI FspAI HpaI KpnI MfeI MluI MmeI MscI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI XhoI XmaI XmnI ZraI