################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BamHI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI DraIII Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MboI MfeI MflI MluI MroI MseI MunI NcoI NdeI NdeII NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfuI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AfaI 456 GTAC GT^AC AflIII 1007 ACACGT A^C[GA][CT]GT AgeI 37 ACCGGT A^CCGGT ApaI 762 GGGCCC GGGCC^C BbrPI 1008 CACGTG CAC^GTG BbsI 874 GTCTTC GAAGAC(2/6) BbvCI 231 CCTCAGC CCTCAGC(-5/-2) BpuAI 874 GTCTTC GAAGAC(2/6) BsaAI 1008 CACGTG [CT]AC^GT[GA] BsaWI 37 ACCGGT [AT]^CCGG[AT] BsiEI 1073 CGGCCG CG[GA][CT]^CG BsmAI 839 GTCTC GTCTC(1/5) BsmBI 838 CGTCTC CGTCTC(1/5) BsrBI 1032 CCGCTC CCGCTC(-3/-3) BstAPI 702 GCACCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraII 762 GGGCCCC [GA]G^G[ACGT]CC[CT] DrdI 229 GACCTCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1073 CGGCCG C^GGCCG EarI 67 GAAGAG CTCTTC(1/4) EclXI 1073 CGGCCG C^GGCCG Eco52I 1073 CGGCCG C^GGCCG EcoO109I 762 GGGCCCC [GA]G^G[ACGT]CC[CT] Ksp632I 67 GAAGAG CTCTTC(1/4) MaeII 1009 ACGT A^CGT MaeIII 102 GTCAC ^GT[ACGT]AC NaeI 86 GCCGGC GCC^GGC NgoMIV 86 GCCGGC G^CCGGC NotI 1072 GCGGCCGC GC^GGCCGC NspI 479 GCATGC [GA]CATG^[CT] PinAI 37 ACCGGT A^CCGGT PmaCI 1008 CACGTG CAC^GTG PmlI 1008 CACGTG CAC^GTG PstI 184 CTGCAG CTGCA^G PvuII 960 CAGCTG CAG^CTG RsaI 456 GTAC GT^AC SfcI 184 CTGCAG C^T[GA][CT]AG SgrAI 36 CACCGGTG C[GA]^CCGG[CT]G SphI 479 GCATGC GCATG^C TfiI 566 GAATC G^A[AT]TC Tsp45I 102 GTCAC ^GT[GC]AC XcmI 887 CCACGCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AspHI 268 GTGCTC G[AT]GC[AT]^C 682 GTGCTC BglI 589 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 764 GCCCCGGTGGC BsiHKAI 268 GTGCTC G[AT]GC[AT]^C 682 GTGCTC BspMI 416 ACCTGC ACCTGC(4/8) 695 ACCTGC BstXI 143 CCAACGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 895 CCACTGGTGTGG HphI 906 GGTGA GGTGA(8/7) 425 TCACC MboII 67 GAAGA GAAGA(8/7) 875 TCTTC NlaIII 150 CATG CATG^ 480 CATG PleI 368 GACTC GAGTC(4/5) 73 GAGTC SmaI 428 CCCGGG CCC^GGG 741 CCCGGG XmaCI 428 CCCGGG C^CCGGG 741 CCCGGG XmaI 428 CCCGGG C^CCGGG 741 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccII AciI AcyI AluI AlwNI AvaI BalI BanI BanII BbeI BbvI BcnI BmyI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DsaI EaeI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI KasI KspI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIV SacII Sau96I ScrFI SfaNI SfoI TseI TspRI