(Linear) (Six-base) MAPSORT of: KIBB8821.seq Check: 6626 from: 1 to: 1152 KIBB8821.orf With 182 enzymes: SgfI * December 27, 2011 16:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 704 1152 Size: 704 448 AflIII A'CryG_T Cuts at: 0 1007 1152 Size: 1007 145 AgeI A'CCGG_T Cuts at: 0 37 1152 Size: 37 1115 AleI CACnn'nnGTG Cuts at: 0 900 1096 1152 Size: 900 196 56 Fragments arranged by size: 900 196 56 AlwNI CAG_nnn'CTG Cuts at: 0 239 453 801 897 1152 Size: 239 214 348 96 255 Fragments arranged by size: 348 255 239 214 96 ApaI G_GGCC'C Cuts at: 0 766 1152 Size: 766 386 AvaI C'yCGr_G Cuts at: 0 271 428 741 1152 Size: 271 157 313 411 Fragments arranged by size: 411 313 271 157 BanI G'GyrC_C Cuts at: 0 432 464 537 550 745 772 936 1126 Size: 432 32 73 13 195 27 164 190 Cuts at: 1126 1152 Size: 26 Fragments arranged by size: 432 195 190 164 73 32 27 26 13 BanII G_rGCy'C Cuts at: 0 278 302 766 1152 Size: 278 24 464 386 Fragments arranged by size: 464 386 278 24 BbeI G_GCGC'C Cuts at: 0 436 468 554 749 776 1152 Size: 436 32 86 195 27 376 Fragments arranged by size: 436 376 195 86 32 27 BbsI GAAGACnn'nnnn_ Cuts at: 0 867 1152 Size: 867 285 BbvCI CC'TCA_GC Cuts at: 0 232 1152 Size: 232 920 BglI GCCn_nnn'nGGC Cuts at: 0 595 770 1152 Size: 595 175 382 Fragments arranged by size: 595 382 175 Bme1580I G_kGCm'C Cuts at: 0 136 540 766 831 941 1103 1129 1152 Size: 136 404 226 65 110 162 26 23 Fragments arranged by size: 404 226 162 136 110 65 26 23 BmrI ACTGGGnnnn_n' Cuts at: 0 321 1132 1152 Size: 321 811 20 Fragments arranged by size: 811 321 20 Bpu10I CC'TnA_GC Cuts at: 0 232 1152 Size: 232 920 BsaAI yAC'GTr Cuts at: 0 1010 1152 Size: 1010 142 BsaHI Gr'CG_yC Cuts at: 0 433 465 551 746 773 837 1058 1152 Size: 433 32 86 195 27 64 221 94 Fragments arranged by size: 433 221 195 94 86 64 32 27 BsaWI w'CCGG_w Cuts at: 0 37 1152 Size: 37 1115 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 28 1152 Size: 28 1124 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 3 18 85 373 1036 1152 Size: 3 15 67 288 663 116 Fragments arranged by size: 663 288 116 67 15 3 BseYI C'CCAG_C Cuts at: 0 322 357 749 794 1152 Size: 322 35 392 45 358 Fragments arranged by size: 392 358 322 45 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 610 685 799 970 1152 Size: 610 75 114 171 182 Fragments arranged by size: 610 182 171 114 75 BsiEI CG_ry'CG Cuts at: 0 1076 1152 Size: 1076 76 BsiHKAI G_wGCw'C Cuts at: 0 272 686 1152 Size: 272 414 466 Fragments arranged by size: 466 414 272 BsmBI CGTCTCn'nnnn_ Cuts at: 0 844 1152 Size: 844 308 Bsp1286I G_dGCh'C Cuts at: 0 136 272 278 302 540 686 766 831 Size: 136 136 6 24 238 146 80 65 Cuts at: 831 941 1103 1129 1152 Size: 110 162 26 23 Fragments arranged by size: 238 162 146 136 136 110 80 65 26 24 23 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 425 704 1152 Size: 425 279 448 Fragments arranged by size: 448 425 279 BsrBI CCG'CTC Cuts at: 0 1034 1152 Size: 1034 118 BsrFI r'CCGG_y Cuts at: 0 37 86 286 933 1152 Size: 37 49 200 647 219 Fragments arranged by size: 647 219 200 49 37 BssSI C'ACGA_G Cuts at: 0 163 397 514 1152 Size: 163 234 117 638 Fragments arranged by size: 638 234 163 117 BstAPI GCAn_nnn'nTGC Cuts at: 0 708 1152 Size: 708 444 BstXI CCAn_nnnn'nTGG Cuts at: 0 150 902 1152 Size: 150 752 250 Fragments arranged by size: 752 250 150 BtgI C'CryG_G Cuts at: 0 541 593 820 1136 1152 Size: 541 52 227 316 16 Fragments arranged by size: 541 316 227 52 16 BtsI GCAGTG_nn' Cuts at: 0 390 627 1112 1152 Size: 390 237 485 40 Fragments arranged by size: 485 390 237 40 DrdI GACnn_nn'nnGTC Cuts at: 0 235 1152 Size: 235 917 EaeI y'GGCC_r Cuts at: 0 122 140 587 884 914 956 1073 1152 Size: 122 18 447 297 30 42 117 79 Fragments arranged by size: 447 297 122 117 79 42 30 18 EagI C'GGCC_G Cuts at: 0 1073 1152 Size: 1073 79 EarI CTCTTCn'nnn_ Cuts at: 0 62 1152 Size: 62 1090 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1018 1152 Size: 1018 134 EcoO109I rG'GnC_Cy Cuts at: 0 763 1152 Size: 763 389 HaeII r_GCGC'y Cuts at: 0 323 436 454 468 490 502 554 583 Size: 323 113 18 14 22 12 52 29 Cuts at: 583 601 749 776 1152 Size: 18 148 27 376 Fragments arranged by size: 376 323 148 113 52 29 27 22 18 18 14 12 KasI G'GCGC_C Cuts at: 0 432 464 550 745 772 1152 Size: 432 32 86 195 27 380 Fragments arranged by size: 432 380 195 86 32 27 MscI TGG'CCA Cuts at: 0 124 142 886 916 1152 Size: 124 18 744 30 236 Fragments arranged by size: 744 236 124 30 18 MslI CAynn'nnrTG Cuts at: 0 97 900 1096 1152 Size: 97 803 196 56 Fragments arranged by size: 803 196 97 56 MspA1I CmG'CkG Cuts at: 0 543 595 822 834 845 920 962 999 Size: 543 52 227 12 11 75 42 37 Cuts at: 999 1078 1138 1152 Size: 79 60 14 Fragments arranged by size: 543 227 79 75 60 52 42 37 14 12 11 NaeI GCC'GGC Cuts at: 0 88 1152 Size: 88 1064 NarI GG'CG_CC Cuts at: 0 433 465 551 746 773 1152 Size: 433 32 86 195 27 379 Fragments arranged by size: 433 379 195 86 32 27 NgoMIV G'CCGG_C Cuts at: 0 86 1152 Size: 86 1066 NotI GC'GGCC_GC Cuts at: 0 1073 1152 Size: 1073 79 NspI r_CATG'y Cuts at: 0 483 1152 Size: 483 669 PasI CC'CwG_GG Cuts at: 0 507 533 941 1152 Size: 507 26 408 211 Fragments arranged by size: 507 408 211 26 PmlI CAC'GTG Cuts at: 0 1010 1152 Size: 1010 142 PspOMI G'GGCC_C Cuts at: 0 762 1152 Size: 762 390 PstI C_TGCA'G Cuts at: 0 188 1152 Size: 188 964 PvuII CAG'CTG Cuts at: 0 962 1152 Size: 962 190 SacII CC_GC'GG Cuts at: 0 544 596 823 1139 1152 Size: 544 52 227 316 13 Fragments arranged by size: 544 316 227 52 13 SfcI C'TryA_G Cuts at: 0 184 1152 Size: 184 968 SfoI GGC'GCC Cuts at: 0 434 466 552 747 774 1152 Size: 434 32 86 195 27 378 Fragments arranged by size: 434 378 195 86 32 27 SgrAI Cr'CCGG_yG Cuts at: 0 37 1152 Size: 37 1115 SmaI CCC'GGG Cuts at: 0 430 743 1152 Size: 430 313 409 Fragments arranged by size: 430 409 313 SphI G_CATG'C Cuts at: 0 483 1152 Size: 483 669 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 894 1152 Size: 894 258 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 359 391 551 583 1152 Size: 359 32 160 32 569 Fragments arranged by size: 569 359 160 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 894 1152 Size: 894 258 XmaI C'CCGG_G Cuts at: 0 428 741 1152 Size: 428 313 411 Fragments arranged by size: 428 411 313 Enzymes that do cut: AarI AflIII AgeI AleI AlwNI ApaI AvaI BanI BanII BbeI BbsI BbvCI BglI Bme1580I BmrI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstXI BtgI BtsI DrdI EaeI EagI EarI EciI EcoO109I HaeII KasI MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI PasI PmlI PspOMI PstI PvuII SacII SfcI SfoI SgrAI SmaI SphI TaqII TstI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AfeI AflII AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI BpmI BpuEI BsaI BsaBI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI NheI NruI NsiI PacI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SmlI SnaBI SpeI SrfI SspI StuI StyI SwaI TatI Tth111I XbaI XhoI XmnI ZraI