################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BanI BanII BbeI BbvCI BcgI BciVI BclI BcnI BfrI BlnI BlpI BmyI Bpu1102I BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I FauI FbaI FseI FspI HaeIII HapII HincII HindII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MslI MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I SspBI SspI StuI SwaI TspRI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccII 1834 CGCG CG^CG AcyI 597 GGCGTC G[GA]^CG[CT]C AlwNI 634 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 1920 AGCGCT AGC^GCT BamHI 156 GGATCC G^GATCC BbrPI 1413 CACGTG CAC^GTG BglI 661 GCCTTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 264 AGATCT A^GATCT BpmI 332 CTCCAG CTGGAG(16/14) BsaAI 1413 CACGTG [CT]AC^GT[GA] BsaHI 597 GGCGTC G[GA]^CG[CT]C BsgI 1490 CTGCAC GTGCAG(16/14) BsrDI 276 GCAATG GCAATG(2/0) BssSI 931 CACGAG CACGAG(-5/-1) BstBI 1307 TTCGAA TT^CGAA BstF5I 738 CATCC GGATG(2/0) BstUI 1834 CGCG CG^CG CfoI 1921 GCGC GCG^C DraII 1470 GGGACCT [GA]G^G[ACGT]CC[CT] Eco47III 1920 AGCGCT AGC^GCT EcoNI 1657 CCTGGTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1470 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 906 GAATTC G^AATTC EcoRV 1632 GATATC GAT^ATC EcoT14I 307 CCAAGG C^C[AT][AT]GG EcoT22I 1706 ATGCAT ATGCA^T FokI 738 CATCC GGATG(9/13) HaeII 1920 AGCGCT [GA]GCGC^[CT] HhaI 1921 GCGC GCG^C Hin1I 597 GGCGTC G[GA]^CG[CT]C HinP1I 1921 GCGC G^CGC HindIII 1102 AAGCTT A^AGCTT MspA1I 868 CAGCGG C[AC]G^C[GT]G MvnI 1834 CGCG CG^CG NsiI 1706 ATGCAT ATGCA^T NspI 291 ACATGC [GA]CATG^[CT] NspV 1307 TTCGAA TT^CGAA PflMI 1567 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1413 CACGTG CAC^GTG PmlI 1413 CACGTG CAC^GTG PpuMI 1470 GGGACCT [GA]G^G[AT]CC[CT] PstI 1130 CTGCAG CTGCA^G SfuI 1307 TTCGAA TT^CGAA SmlI 284 CTCAAG C^T[CT][GA]AG SpeI 24 ACTAGT A^CTAGT StyI 307 CCAAGG C^C[AT][AT]GG TfiI 1792 GAATC G^A[AT]TC Tsp45I 600 GTCAC ^GT[GC]AC Van91I 1567 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1869 CCATCAGAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 232 GTAGAC GT^[AC][GT]AC 1553 GTAGAC AfaI 281 GTAC GT^AC 1090 GTAC Asp700I 193 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1614 GAATACCTTC AvaII 1002 GGACC G^G[AT]CC 1471 GGACC BbsI 224 GTCTTC GAAGAC(2/6) 1935 GTCTTC BpuAI 224 GTCTTC GAAGAC(2/6) 1935 GTCTTC BsmFI 1470 GGGAC GGGAC(10/14) 28 GTCCC BspMI 357 ACCTGC ACCTGC(4/8) 36 GCAGGT BsrI 531 ACTGG ACTGG(1/-1) 112 CCAGT Cfr13I 1002 GGACC G^G[ACGT]CC 1471 GGACC DraI 207 TTTAAA TTT^AAA 366 TTTAAA HgaI 598 GCGTC GACGC(5/10) 1933 GCGTC MaeII 947 ACGT A^CGT 1414 ACGT NlaIII 292 CATG CATG^ 694 CATG NlaIV 156 GGATCC GG[ACGT]^[ACGT]CC 1470 GGGACC PleI 814 GACTC GAGTC(4/5) 1838 GACTC RsaI 281 GTAC GT^AC 1090 GTAC Sau96I 1002 GGACC G^G[ACGT]CC 1471 GGACC TaqI 1308 TCGA T^CGA 1630 TCGA TthHB8I 1308 TCGA T^CGA 1630 TCGA XmnI 193 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1614 GAATACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI BbvI BfaI BsaJI BseRI BsiYI BslI BsmAI BsmI BssKI BstNI BstYI Cac8I DdeI DpnII EarI EcoRII Fnu4HI HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII Sau3AI ScrFI SfaNI SfcI Tru9I TseI Tsp509I XhoII