Restriction map for
pFN21AE2372
(Circular) (Six-base) MAPSORT of: pFN21AE2372.seq Check: 3258 from: 1 to: 6676
pFN21AE2372 6676 bp
With 182 enzymes: SgfI *
October 11, 2011 14:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2032 2371 2032
Size: 339 6337
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4486 278
Size: 53 83 186 936 2950 2468
Fragments arranged by size:
2950 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2238 3559 4019 1937
Size: 301 1321 460 4594
Fragments arranged by size:
4594 1321 460 301
Acc65I G'GTAC_C
Cuts at: 3997 3997
Size: 6676
AclI AA'CG_TT
Cuts at: 1563 4803 5176 1563
Size: 3240 373 3063
Fragments arranged by size:
3240 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2261 3167 3830 3927 4739 6123 6391 1632
Size: 629 906 663 97 812 1384 268 1917
Fragments arranged by size:
1917 1384 906 812 663 629 268 97
AfeI AGC'GCT
Cuts at: 3927 3927
Size: 6676
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6479
AflIII A'CryG_T
Cuts at: 1203 5575 6552 1203
Size: 4372 977 1327
Fragments arranged by size:
4372 1327 977
AgeI A'CCGG_T
Cuts at: 5507 5507
Size: 6676
AhdI GACnn_n'nnGTC
Cuts at: 5405 5405
Size: 6676
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6676
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6676
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3663 3695 3663
Size: 32 6644
AlwNI CAG_nnn'CTG
Cuts at: 2644 4419 5991 2644
Size: 1775 1572 3329
Fragments arranged by size:
3329 1775 1572
ApaLI G'TGCA_C
Cuts at: 4733 5889 4733
Size: 1156 5520
ApoI r'AATT_y
Cuts at: 1493 2216 2261 2307 2456 2911 3528 3985 4211
Size: 723 45 46 149 455 617 457 226
Cuts at: 4211 4363 6472 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 723 617 457 455 226 152
149 46 45
AseI AT'TA_AT
Cuts at: 160 1032 5230 160
Size: 872 4198 1606
Fragments arranged by size:
4198 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6676
AvaI C'yCGr_G
Cuts at: 1870 1943 4001 1870
Size: 73 2058 4545
Fragments arranged by size:
4545 2058 73
BamHI G'GATC_C
Cuts at: 2161 4006 4373 2161
Size: 1845 367 4464
Fragments arranged by size:
4464 1845 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3997 5452 618
Size: 325 217 627 2210 1455 1842
Fragments arranged by size:
2210 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3995 1391
Size: 2604 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2222 3933 928
Size: 971 323 1711 3671
Fragments arranged by size:
3671 1711 971 323
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4867 4901 1903
Size: 34 2930 34 3678
Fragments arranged by size:
3678 2930 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4568 5778 4568
Size: 1210 5466
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6676
BfrBI ATG'CAT
Cuts at: 3713 3713
Size: 6676
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2672 5287 136
Size: 107 122 71 631 605 1000 2615 1525
Fragments arranged by size:
2615 1525 1000 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2269 6671 2269
Size: 4402 2274
BlpI GC'TnA_GC
Cuts at: 4093 4093
Size: 6676
Bme1580I G_kGCm'C
Cuts at: 1790 4737 5893 1790
Size: 2947 1156 2573
Fragments arranged by size:
2947 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6676
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4407 5365 442
Size: 416 53 83 399 24 2990 958 1753
Fragments arranged by size:
2990 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6676
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2322 5336 1239
Size: 1083 3014 2579
Fragments arranged by size:
3014 2579 1083
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2274 4801 5666 5964 2274
Size: 2527 865 298 2986
Fragments arranged by size:
2986 2527 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5339 882
Size: 617 3840 2219
Fragments arranged by size:
3840 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3420 4467 493
Size: 711 2216 1047 2702
Fragments arranged by size:
2702 2216 1047 711
BsaBI GATnn'nnATC
Cuts at: 6670 6670
Size: 6676
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2603 4483 4865 5500
Size: 53 83 186 936 1070 1880 382 635
Cuts at: 5500 275
Size: 1451
Fragments arranged by size:
1880 1451 1070 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5109 5507 5781 5928 6339 5109
Size: 398 274 147 411 5446
Fragments arranged by size:
5446 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2062 2092 1178
Size: 30 165 30 659 30 5762
Fragments arranged by size:
5762 659 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 2083 2879 3589 1186
Size: 892 5 796 710 4273
Fragments arranged by size:
4273 892 796 710 5
BseYI C'CCAG_C
Cuts at: 5879 6375 5879
Size: 496 6180
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3480 1163
Size: 573 1744 4359
Fragments arranged by size:
4359 1744 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4126 4887 5915 1540
Size: 275 186 2125 761 1028 2301
Fragments arranged by size:
2301 2125 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4737 4822 5893 4737
Size: 85 1071 5520
Fragments arranged by size:
5520 1071 85
BsmI GAATG_Cn'
Cuts at: 2632 2936 3611 3828 4187 4280 2632
Size: 304 675 217 359 93 5028
Fragments arranged by size:
5028 675 359 304 217 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3995 4737 4822 5893 1391
Size: 399 2205 742 85 1071 2174
Fragments arranged by size:
2205 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6339 6339
Size: 6676
BspHI T'CATG_A
Cuts at: 4565 6295 4565
Size: 1730 4946
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2032 2371 4031 844
Size: 1188 339 1660 3489
Fragments arranged by size:
3489 1660 1188 339
BsrBI CCG'CTC
Cuts at: 1394 1823 4563 1394
Size: 429 2740 3507
Fragments arranged by size:
3507 2740 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2288 5171 5345 65
Size: 1183 1040 2883 174 1396
Fragments arranged by size:
2883 1396 1183 1040 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5320 5507 1700
Size: 110 3510 187 2869
Fragments arranged by size:
3510 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6676
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6676
BssSI C'ACGA_G
Cuts at: 2936 4736 5748 2936
Size: 1800 1012 3864
Fragments arranged by size:
3864 1800 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6676
BstBI TT'CG_AA
Cuts at: 3313 5480 3313
Size: 2167 4509
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6676
BstYI r'GATC_y
Cuts at: 1972 2161 2269 3181 4006 4373 4758 4775 6227
Size: 189 108 912 825 367 385 17 1452
Cuts at: 6227 6313 6325 6671 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 912 825 385 367 346 189
108 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5937
Fragments arranged by size:
5937 683 56
BtsI GCAGTG_nn'
Cuts at: 4192 4986 5006 4192
Size: 794 20 5862
Fragments arranged by size:
5862 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6676
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6641
DraI TTT'AAA
Cuts at: 2214 2373 3980 2214
Size: 159 1607 4910
Fragments arranged by size:
4910 1607 159
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5683 809
Size: 507 4367 1802
Fragments arranged by size:
4367 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4123 5012 6641
Size: 54 1425 50 275 18 2293 889 1629
Cuts at: 6641 8
Size: 43
Fragments arranged by size:
2293 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4123 1537
Size: 275 2311 4090
Fragments arranged by size:
4090 2311 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2856 2899 3315 4606 1353
Size: 1503 43 416 1291 3423
Fragments arranged by size:
3423 1503 1291 416 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3480 5263 5637 5783 387
Size: 1064 138 1891 1783 374 146 1280
Fragments arranged by size:
1891 1783 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2261 2322 3167 3830 3927 4739 5336
Size: 393 629 61 845 663 97 812 597
Cuts at: 5336 6123 6391 1239
Size: 787 268 1524
Fragments arranged by size:
1524 845 812 787 663 629 597 393
268 97 61
EcoNI CCTnn'n_nnAGG
Cuts at: 3666 3666
Size: 6676
EcoO109I rG'GnC_Cy
Cuts at: 3476 3476
Size: 6676
EcoRI G'AATT_C
Cuts at: 2911 3985 4363 2911
Size: 1074 378 5224
Fragments arranged by size:
5224 1074 378
EcoRV GAT'ATC
Cuts at: 3639 3639
Size: 6676
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2864 2864
Size: 6676
FspI TGC'GCA
Cuts at: 5182 5182
Size: 6676
HaeII r_GCGC'y
Cuts at: 1678 3929 5823 1678
Size: 2251 1894 2531
Fragments arranged by size:
2531 2251 1894
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3574 3606 3716 3748 5317
Size: 32 135 32 2002 32 110 32 1569
Cuts at: 5317 5349 5391 5423 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2002 1569 135 110 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4020 4266 669
Size: 988 281 2082 246 3079
Fragments arranged by size:
3079 2082 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3107 748
Size: 2359 4317
HpaI GTT'AAC
Cuts at: 4266 4266
Size: 6676
KpnI G_GTAC'C
Cuts at: 4001 4001
Size: 6676
MfeI C'AATT_G
Cuts at: 4275 4275
Size: 6676
MluI A'CGCG_T
Cuts at: 6552 6552
Size: 6676
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3280 3774 5790 5974 1305
Size: 1975 494 2016 184 2007
Fragments arranged by size:
2016 2007 1975 494 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4854
Fragments arranged by size:
4854 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4634 4993 5152 518
Size: 648 3468 359 159 2042
Fragments arranged by size:
3468 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2875 4092 4769 5917 6162 1606
Size: 1269 1217 677 1148 245 2120
Fragments arranged by size:
2120 1269 1217 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6124
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6676
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6676
NotI GC'GGCC_GC
Cuts at: 4123 4123
Size: 6676
NsiI A_TGCA'T
Cuts at: 3715 3715
Size: 6676
NspI r_CATG'y
Cuts at: 1132 1229 2300 4034 5579 1132
Size: 97 1071 1734 1545 2229
Fragments arranged by size:
2229 1734 1545 1071 97
PasI CC'CwG_GG
Cuts at: 1348 3956 1348
Size: 2608 4068
PciI A'CATG_T
Cuts at: 5575 5575
Size: 6676
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3578 1108
Size: 2470 4206
PfoI T'CCnGG_A
Cuts at: 3048 3048
Size: 6676
PmeI GTTT'AAAC
Cuts at: 3980 3980
Size: 6676
PmlI CAC'GTG
Cuts at: 3420 3420
Size: 6676
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4745 4777 6258 6290 4745
Size: 32 1481 32 5131
Fragments arranged by size:
5131 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3476 3476
Size: 6676
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6676
PsiI TTA'TAA
Cuts at: 4246 4246
Size: 6676
PstI C_TGCA'G
Cuts at: 830 3139 4028 830
Size: 2309 889 3478
Fragments arranged by size:
3478 2309 889
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6676
SalI G'TCGA_C
Cuts at: 1936 4018 1936
Size: 2082 4594
SbfI CC_TGCA'GG
Cuts at: 4028 4028
Size: 6676
ScaI AGT'ACT
Cuts at: 4924 6636 4924
Size: 1712 4964
SfcI C'TryA_G
Cuts at: 826 1045 2324 2870 3135 3360 4024 5159 5840
Size: 219 1279 546 265 225 664 1135 681
Cuts at: 5840 6031 826
Size: 191 1471
Fragments arranged by size:
1471 1279 1135 681 664 546 265 225
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6676
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6676
SmaI CCC'GGG
Cuts at: 1872 4003 1872
Size: 2131 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2289 4780 5681 5943 820
Size: 197 926 346 2491 901 262 1553
Fragments arranged by size:
2491 1553 926 901 346 262 197
SnaBI TAC'GTA
Cuts at: 493 4467 493
Size: 3974 2702
SpeI A'CTAG_T
Cuts at: 152 2029 152
Size: 1877 4799
SphI G_CATG'C
Cuts at: 1132 4034 1132
Size: 2902 3774
SspI AAT'ATT
Cuts at: 5 52 4600 5
Size: 47 4548 2081
Fragments arranged by size:
4548 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6676
StyI C'CwwG_G
Cuts at: 513 1065 2312 4115 513
Size: 552 1247 1803 3074
Fragments arranged by size:
3074 1803 1247 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4703 4720 4873 5058 5503 325
Size: 1255 3123 17 153 185 445 1498
Fragments arranged by size:
3123 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2285 3094 4922
Size: 274 80 33 51 1443 308 809 1828
Cuts at: 4922 6634 96
Size: 1712 138
Fragments arranged by size:
1828 1712 1443 809 308 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2427 2459 6636 6668 2427
Size: 32 4177 32 2435
Fragments arranged by size:
4177 2435 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6676
XbaI T'CTAG_A
Cuts at: 4012 4012
Size: 6676
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3881 3881
Size: 6676
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6676
XmaI C'CCGG_G
Cuts at: 1870 4001 1870
Size: 2131 4545
XmnI GAAnn'nnTTC
Cuts at: 2202 3623 4805 2202
Size: 1421 1182 4073
Fragments arranged by size:
4073 1421 1182
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4484 276
Size: 53 83 186 936 2950 2468
Fragments arranged by size:
2950 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI