################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BbvI BcgI BcnI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FokI FseI FspI HapII HgaI Hin1I HindIII HpaI HpaII KasI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MslI MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TseI Tsp45I Tth111I TthHB8I Van91I XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### Acc65I 487 GGTACC G^GTACC AfaI 488 GTAC GT^AC AlwI 646 GATCC GGATC(4/5) ApaI 41 GGGCCC GGGCC^C Asp718I 487 GGTACC G^GTACC BanII 41 GGGCCC G[GA]GC[CT]^C BciVI 137 GTATCC GTATCC(6/5) BclI 374 TGATCA T^GATCA BglI 729 GCCGCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BseRI 121 GAGGAG GAGGAG(10/8) BspHI 129 TCATGA T^CATGA BsrDI 378 CATTGC GCAATG(2/0) BssKI 33 CCTGG ^CC[ACGT]GG BssSI 718 CACGAG CACGAG(-5/-1) BstNI 33 CCTGG CC^[AT]GG DdeI 456 CTTAG C^T[ACGT]AG EaeI 20 CGGCCA [CT]^GGCC[GA] EcoRII 33 CCTGG ^CC[AT]GG FbaI 374 TGATCA T^GATCA HaeII 27 GGCGCT [GA]GCGC^[CT] HphI 385 TCACC GGTGA(8/7) KpnI 487 GGTACC GGTAC^C MaeIII 554 GTAAC ^GT[ACGT]AC MvaI 33 CCTGG CC^[AT]GG PleI 636 GACTC GAGTC(4/5) PstI 58 CTGCAG CTGCA^G RcaI 129 TCATGA T^CATGA RsaI 488 GTAC GT^AC SapI 685 GCTCTTC GCTCTTC(1/4) ScrFI 33 CCTGG CC^[ACGT]GG SfaNI 585 GATGC GCATC(5/9) SfcI 58 CTGCAG C^T[GA][CT]AG XbaI 498 TCTAGA T^CTAGA XcmI 23 CCAAGGCGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BfaI 317 CTAG C^TAG 499 CTAG BsgI 52 CTGCAC GTGCAG(16/14) 323 GTGCAG BsmI 211 GAATGC GAATGC(1/-1) 698 GCATTC BspMI 56 ACCTGC ACCTGC(4/8) 71 ACCTGC BsrI 80 ACTGG ACTGG(1/-1) 437 ACTGG EarI 303 CTCTTC CTCTTC(1/4) 686 CTCTTC FauI 44 CCCGC CCCGC(4/6) 39 GCGGG HincII 328 GTTGAC GT[CT]^[GA]AC 633 GTTGAC HindII 328 GTTGAC GT[CT]^[GA]AC 633 GTTGAC Ksp632I 303 CTCTTC CTCTTC(1/4) 686 CTCTTC MaeI 317 CTAG C^TAG 499 CTAG MseI 407 TTAA T^TAA 563 TTAA TfiI 295 GAATC G^A[AT]TC 367 GATTC Tru9I 407 TTAA T^TAA 563 TTAA TspRI 216 CACTG CA[GC]TG[ACGT][ACGT]^ 529 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AccII AciI AluI Asp700I BanI BmyI BsaJI BsiYI BslI Bsp1286I BstUI Cac8I CfoI Cfr13I DpnII EcoT14I Fnu4HI HaeIII HhaI HinP1I HinfI ItaI MboI MboII MnlI MspA1I MvnI MwoI NdeII NlaIII NlaIV PvuII Sau3AI Sau96I StyI Tsp509I XmnI