(Circular) (Six-base) MAPSORT of: pFN21AE6661.seq Check: 3108 from: 1 to: 5527 pFN21AE6661 5527 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2070 2070 Size: 5527 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3337 278 Size: 53 83 186 936 1801 2468 Fragments arranged by size: 2468 1801 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2870 1937 Size: 933 4594 Acc65I G'GTAC_C Cuts at: 2492 2848 2492 Size: 356 5171 AclI AA'CG_TT Cuts at: 1563 3654 4027 1563 Size: 2091 373 3063 Fragments arranged by size: 3063 2091 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2678 2691 3590 4974 5242 1632 Size: 1046 13 899 1384 268 1917 Fragments arranged by size: 1917 1384 1046 899 268 13 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5330 AflIII A'CryG_T Cuts at: 1203 4426 5403 1203 Size: 3223 977 1327 Fragments arranged by size: 3223 1327 977 AgeI A'CCGG_T Cuts at: 4358 4358 Size: 5527 AhdI GACnn_n'nnGTC Cuts at: 4256 4256 Size: 5527 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5527 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5527 AlwNI CAG_nnn'CTG Cuts at: 3270 4842 3270 Size: 1572 3955 ApaI G_GGCC'C Cuts at: 2050 2050 Size: 5527 ApaLI G'TGCA_C Cuts at: 3584 4740 3584 Size: 1156 4371 ApoI r'AATT_y Cuts at: 1493 2836 3062 3214 5323 1493 Size: 1343 226 152 2109 1697 Fragments arranged by size: 2109 1697 1343 226 152 AseI AT'TA_AT Cuts at: 160 1032 4081 160 Size: 872 3049 1606 Fragments arranged by size: 3049 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5527 AvaI C'yCGr_G Cuts at: 1870 1943 2852 1870 Size: 73 909 4545 Fragments arranged by size: 4545 909 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2513 2546 2513 Size: 33 5494 BamHI G'GATC_C Cuts at: 2857 3224 2857 Size: 367 5160 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2009 2492 2731 2848 4303 Size: 325 217 627 222 483 239 117 1455 Cuts at: 4303 618 Size: 1842 Fragments arranged by size: 1842 1455 627 483 325 239 222 217 117 BanII G_rGCy'C Cuts at: 1391 2050 2846 1391 Size: 659 796 4072 Fragments arranged by size: 4072 796 659 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4556 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3718 3752 1903 Size: 34 1781 34 3678 Fragments arranged by size: 3678 1781 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2153 3419 4629 2153 Size: 1266 1210 3051 Fragments arranged by size: 3051 1266 1210 BclI T'GATC_A Cuts at: 1548 2379 1548 Size: 831 4696 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2740 4138 136 Size: 107 122 71 631 605 1068 1398 1525 Fragments arranged by size: 1525 1398 1068 631 605 122 107 71 BglII A'GATC_T Cuts at: 5522 5522 Size: 5527 BlpI GC'TnA_GC Cuts at: 2944 2944 Size: 5527 Bme1580I G_kGCm'C Cuts at: 1790 2012 2050 2111 2542 3588 4744 1790 Size: 222 38 61 431 1046 1156 2573 Fragments arranged by size: 2573 1156 1046 431 222 61 38 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5527 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2095 2452 3258 Size: 416 53 83 399 24 678 357 806 Cuts at: 3258 4216 442 Size: 958 1753 Fragments arranged by size: 1753 958 806 678 416 399 357 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5527 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4187 1239 Size: 2948 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3652 4517 4815 3652 Size: 865 298 4364 Fragments arranged by size: 4364 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4190 882 Size: 617 2691 2219 Fragments arranged by size: 2691 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3318 493 Size: 711 2114 2702 Fragments arranged by size: 2702 2114 711 BsaBI GATnn'nnATC Cuts at: 5521 5521 Size: 5527 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3334 3716 4351 275 Size: 53 83 186 936 1801 382 635 1451 Fragments arranged by size: 1801 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3960 4358 4632 4779 5190 3960 Size: 398 274 147 411 4297 Fragments arranged by size: 4297 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5302 Fragments arranged by size: 5302 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2141 1186 Size: 955 4572 BseYI C'CCAG_C Cuts at: 4730 5226 4730 Size: 496 5031 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2042 2349 1163 Size: 573 306 307 4341 Fragments arranged by size: 4341 573 307 306 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2977 3738 4766 1540 Size: 275 186 976 761 1028 2301 Fragments arranged by size: 2301 1028 976 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3588 3673 4744 3588 Size: 85 1071 4371 Fragments arranged by size: 4371 1071 85 BsmI GAATG_Cn' Cuts at: 2222 2703 3038 3131 2222 Size: 481 335 93 4618 Fragments arranged by size: 4618 481 335 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2050 2111 2542 2846 3588 3673 Size: 399 222 38 61 431 304 742 85 Cuts at: 3673 4744 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 431 399 304 222 85 61 38 BspEI T'CCGG_A Cuts at: 5190 5190 Size: 5527 BspHI T'CATG_A Cuts at: 2134 3416 5146 2134 Size: 1282 1730 2515 Fragments arranged by size: 2515 1730 1282 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2070 2085 2882 844 Size: 1226 15 797 3489 Fragments arranged by size: 3489 1226 797 15 BsrBI CCG'CTC Cuts at: 1394 1823 3414 1394 Size: 429 1591 3507 Fragments arranged by size: 3507 1591 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2382 4022 4196 65 Size: 1183 1134 1640 174 1396 Fragments arranged by size: 1640 1396 1183 1134 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4171 4358 1700 Size: 110 2361 187 2869 Fragments arranged by size: 2869 2361 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5527 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5527 BssSI C'ACGA_G Cuts at: 2723 3587 4599 2723 Size: 864 1012 3651 Fragments arranged by size: 3651 1012 864 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5527 BstBI TT'CG_AA Cuts at: 4331 4331 Size: 5527 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5527 BstYI r'GATC_y Cuts at: 1972 2857 3224 3609 3626 5078 5164 5176 5522 Size: 885 367 385 17 1452 86 12 346 Cuts at: 5522 1972 Size: 1977 Fragments arranged by size: 1977 1452 885 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4036 Fragments arranged by size: 4036 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4788 Fragments arranged by size: 4788 683 56 BtsI GCAGTG_nn' Cuts at: 3043 3837 3857 3043 Size: 794 20 4713 Fragments arranged by size: 4713 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5527 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2583 2618 529 Size: 35 2019 35 3438 Fragments arranged by size: 3438 2019 35 35 DraI TTT'AAA Cuts at: 2831 2831 Size: 5527 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4534 809 Size: 507 3218 1802 Fragments arranged by size: 3218 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2974 3863 Size: 54 1425 50 275 18 195 949 889 Cuts at: 3863 5492 8 Size: 1629 43 Fragments arranged by size: 1629 1425 949 889 275 195 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2974 1537 Size: 275 1162 4090 Fragments arranged by size: 4090 1162 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2314 2697 3457 1353 Size: 961 383 760 3423 Fragments arranged by size: 3423 961 760 383 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4114 4488 4634 387 Size: 1064 138 2525 374 146 1280 Fragments arranged by size: 2525 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2678 2691 3590 4187 4974 5242 1239 Size: 393 1046 13 899 597 787 268 1524 Fragments arranged by size: 1524 1046 899 787 597 393 268 13 EcoRI G'AATT_C Cuts at: 2836 3214 2836 Size: 378 5149 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2588 2681 2708 2588 Size: 93 27 5407 Fragments arranged by size: 5407 93 27 FspI TGC'GCA Cuts at: 4033 4033 Size: 5527 HaeII r_GCGC'y Cuts at: 1678 2036 4674 1678 Size: 358 2638 2531 Fragments arranged by size: 2638 2531 358 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2766 2798 4168 4200 4242 Size: 32 135 32 1194 32 1370 32 42 Cuts at: 4242 4274 1373 Size: 32 2626 Fragments arranged by size: 2626 1370 1194 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2335 2640 2871 3117 669 Size: 988 281 397 305 231 246 3079 Fragments arranged by size: 3079 988 397 305 281 246 231 HindIII A'AGCT_T Cuts at: 748 748 Size: 5527 HpaI GTT'AAC Cuts at: 3117 3117 Size: 5527 KpnI G_GTAC'C Cuts at: 2496 2852 2496 Size: 356 5171 MfeI C'AATT_G Cuts at: 3126 3126 Size: 5527 MluI A'CGCG_T Cuts at: 5403 5403 Size: 5527 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2499 4641 4825 1305 Size: 1194 2142 184 2007 Fragments arranged by size: 2142 2007 1194 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3705 Fragments arranged by size: 3705 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3485 3844 4003 518 Size: 648 2319 359 159 2042 Fragments arranged by size: 2319 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2368 2527 2635 2737 2943 3620 4768 5013 Size: 762 159 108 102 206 677 1148 245 Cuts at: 5013 1606 Size: 2120 Fragments arranged by size: 2120 1148 762 677 245 206 159 108 102 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4036 Fragments arranged by size: 4036 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5527 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5527 NotI GC'GGCC_GC Cuts at: 2974 2974 Size: 5527 NspI r_CATG'y Cuts at: 1132 1229 2885 4430 1132 Size: 97 1656 1545 2229 Fragments arranged by size: 2229 1656 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5527 PciI A'CATG_T Cuts at: 4426 4426 Size: 5527 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5527 PmeI GTTT'AAAC Cuts at: 2831 2831 Size: 5527 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3596 3628 5109 5141 3596 Size: 32 1481 32 3982 Fragments arranged by size: 3982 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5527 PsiI TTA'TAA Cuts at: 3097 3097 Size: 5527 PspOMI G'GGCC_C Cuts at: 2046 2046 Size: 5527 PstI C_TGCA'G Cuts at: 830 2067 2879 830 Size: 1237 812 3478 Fragments arranged by size: 3478 1237 812 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5527 PvuII CAG'CTG Cuts at: 2368 2527 2635 2368 Size: 159 108 5260 Fragments arranged by size: 5260 159 108 SalI G'TCGA_C Cuts at: 1936 2869 1936 Size: 933 4594 SapI GCTCTTCn'nnn_ Cuts at: 2697 2697 Size: 5527 SbfI CC_TGCA'GG Cuts at: 2879 2879 Size: 5527 ScaI AGT'ACT Cuts at: 3775 5487 3775 Size: 1712 3815 SfcI C'TryA_G Cuts at: 826 1045 2063 2875 4010 4691 4882 826 Size: 219 1018 812 1135 681 191 1471 Fragments arranged by size: 1471 1135 1018 812 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5527 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5527 SmaI CCC'GGG Cuts at: 1872 2854 1872 Size: 982 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3631 4532 4794 820 Size: 197 926 1688 901 262 1553 Fragments arranged by size: 1688 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3318 493 Size: 2825 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5527 SphI G_CATG'C Cuts at: 1132 2885 1132 Size: 1753 3774 SspI AAT'ATT Cuts at: 5 52 3451 5 Size: 47 3399 2081 Fragments arranged by size: 3399 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5527 StyI C'CwwG_G Cuts at: 513 1065 2004 2028 2353 2421 2966 513 Size: 552 939 24 325 68 545 3074 Fragments arranged by size: 3074 939 552 545 325 68 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2719 2746 3554 3571 3724 3909 4354 Size: 1255 1139 27 808 17 153 185 445 Cuts at: 4354 325 Size: 1498 Fragments arranged by size: 1498 1255 1139 808 445 185 153 27 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3773 5485 96 Size: 274 80 33 51 1443 1796 1712 138 Fragments arranged by size: 1796 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5487 5519 5487 Size: 32 5495 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5527 XbaI T'CTAG_A Cuts at: 2503 2863 2503 Size: 360 5167 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2035 2035 Size: 5527 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5527 XmaI C'CCGG_G Cuts at: 1870 2852 1870 Size: 982 4545 XmnI GAAnn'nnTTC Cuts at: 2691 2703 2821 3656 2691 Size: 12 118 835 4562 Fragments arranged by size: 4562 835 118 12 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3335 276 Size: 53 83 186 936 1801 2468 Fragments arranged by size: 2468 1801 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI