Restriction map for
pFN21AE4232
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI AspI AvrII
BbrPI BbsI BcgI BclI BfrI
BglI BglII BlnI BpuAI BseAI
BsiWI Bsp1407I BspDI BspEI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HpaI KspI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Acc65I 461 GGTACC G^GTACC
AhdI 532 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2375 GTGCAC G^TGCAC
ApaLI 2375 GTGCAC G^TGCAC
Asp700I 2052 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 461 GGTACC G^GTACC
AspEI 532 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 446 TGCGCA TGC^GCA
BbvCI 1173 GCTGAGG CCTCAGC(-5/-2)
BciVI 1694 GGATAC GTATCC(6/5)
BsaI 1143 GGTCTC GGTCTC(1/5)
BsiEI 468 CGGCCG CG[GA][CT]^CG
BsmBI 1250 CGTCTC CGTCTC(1/5)
BsmI 2277 GAATGC GAATGC(1/-1)
BspHI 1949 TCATGA T^CATGA
BsrBI 1868 GAGCGG CCGCTC(-3/-3)
BstAPI 900 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 726 CCAGCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 1642 GACCCCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 468 CGGCCG C^GGCCG
Eam1105I 532 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 468 CGGCCG C^GGCCG
Eco52I 468 CGGCCG C^GGCCG
FspI 446 TGCGCA TGC^GCA
KpnI 461 GGTACC GGTAC^C
NaeI 713 GCCGGC GCC^GGC
NgoMIV 713 GCCGGC G^CCGGC
RcaI 1949 TCATGA T^CATGA
SacI 1737 GAGCTC GAGCT^C
SapI 983 GAAGAGC GCTCTTC(1/4)
SmaI 818 CCCGGG CCC^GGG
SmlI 1237 CTCAAG C^T[CT][GA]AG
XcmI 677 CCATCCCCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 818 CCCGGG C^CCGGG
XmaI 818 CCCGGG C^CCGGG
XmnI 2052 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 38 enzymes) #
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AflIII 2092 ACATGT A^C[GA][CT]GT
2183 ACATGT
AspHI 1737 GAGCTC G[AT]GC[AT]^C
2375 GTGCAC
BalI 854 TGGCCA TGG^CCA
1814 TGGCCA
BamHI 318 GGATCC G^GATCC
2288 GGATCC
BbeI 736 GGCGCC GGCGC^C
1392 GGCGCC
BfaI 523 CTAG C^TAG
1691 CTAG
BsaAI 700 TACGTG [CT]AC^GT[GA]
1663 CACGTA
BsaBI 118 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
319 GATCCACATC
BsaWI 605 ACCGGA [AT]^CCGG[AT]
2270 ACCGGA
BsgI 502 GTGCAG GTGCAG(16/14)
643 GTGCAG
BsiHKAI 1737 GAGCTC G[AT]GC[AT]^C
2375 GTGCAC
BspLU11I 2092 ACATGT A^CATGT
2183 ACATGT
BsrDI 1824 CATTGC GCAATG(2/0)
1603 GCAATG
BstYI 318 GGATCC [GA]^GATC[CT]
2288 GGATCC
EarI 983 GAAGAG CTCTTC(1/4)
1711 GAAGAG
EcoNI 831 CCTCACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1538 CCTCCTTCAGG
FauI 955 CCCGC CCCGC(4/6)
1209 CCCGC
HincII 1190 GTCAAC GT[CT]^[GA]AC
1417 GTCAAC
HindII 1190 GTCAAC GT[CT]^[GA]AC
1417 GTCAAC
KasI 736 GGCGCC G^GCGCC
1392 GGCGCC
Ksp632I 983 GAAGAG CTCTTC(1/4)
1711 GAAGAG
MaeI 523 CTAG C^TAG
1691 CTAG
MamI 118 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
319 GATCCACATC
MflI 318 GGATCC [GA]^GATC[CT]
2288 GGATCC
MluNI 854 TGGCCA TGG^CCA
1814 TGGCCA
MscI 854 TGGCCA TGG^CCA
1814 TGGCCA
MseI 2146 TTAA T^TAA
2422 TTAA
MspA1I 1777 CAGCTG C[AC]G^C[GT]G
2047 CAGCTG
NarI 736 GGCGCC GG^CGCC
1392 GGCGCC
PshAI 196 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
482 GACAGGTGTC
PstI 5 CTGCAG CTGCA^G
2258 CTGCAG
PvuII 1777 CAGCTG CAG^CTG
2047 CAGCTG
SfoI 736 GGCGCC GGC^GCC
1392 GGCGCC
StuI 629 AGGCCT AGG^CCT
1743 AGGCCT
TaqI 656 TCGA T^CGA
671 TCGA
Tru9I 2146 TTAA T^TAA
2422 TTAA
TthHB8I 656 TCGA T^CGA
671 TCGA
XhoII 318 GGATCC [GA]^GATC[CT]
2288 GGATCC
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AvaI AvaII
BanI BanII BbvI BcnI BlpI
BmyI BpmI Bpu1102I BsaHI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MslI MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI TseI Tsp45I Tsp509I TspRI