(Circular) (Six-base) MAPSORT of: pFN21AE4232.seq Check: 6396 from: 1 to: 7180 pFN21AE4232 7180 bp With 182 enzymes: SgfI * March 28, 2012 13:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4990 278 Size: 53 83 186 936 3454 2468 Fragments arranged by size: 3454 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4523 1937 Size: 2586 4594 Acc65I G'GTAC_C Cuts at: 2466 4501 2466 Size: 2035 5145 AclI AA'CG_TT Cuts at: 1563 5307 5680 1563 Size: 3744 373 3063 Fragments arranged by size: 3744 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2188 2302 2426 3532 3735 3935 4048 4076 Size: 556 114 124 1106 203 200 113 28 Cuts at: 4076 5243 6627 6895 1632 Size: 1167 1384 268 1917 Fragments arranged by size: 1917 1384 1167 1106 556 268 203 200 124 114 113 28 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6983 AflIII A'CryG_T Cuts at: 1203 4097 4188 6079 7056 1203 Size: 2894 91 1891 977 1327 Fragments arranged by size: 2894 1891 1327 977 91 AgeI A'CCGG_T Cuts at: 6011 6011 Size: 7180 AhdI GACnn_n'nnGTC Cuts at: 2542 5909 2542 Size: 3367 3813 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7180 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4091 1184 Size: 2907 4273 AlwNI CAG_nnn'CTG Cuts at: 4923 6495 4923 Size: 1572 5608 ApaLI G'TGCA_C Cuts at: 4380 5237 6393 4380 Size: 857 1156 5167 Fragments arranged by size: 5167 1156 857 ApoI r'AATT_y Cuts at: 1493 2018 2908 4346 4412 4489 4715 4867 6976 Size: 525 890 1438 66 77 226 152 2109 Cuts at: 6976 1493 Size: 1697 Fragments arranged by size: 2109 1697 1438 890 525 226 152 77 66 AseI AT'TA_AT Cuts at: 160 1032 5734 160 Size: 872 4702 1606 Fragments arranged by size: 4702 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7180 AvaI C'yCGr_G Cuts at: 1870 1943 2396 2823 2898 3061 4505 1870 Size: 73 453 427 75 163 1444 4545 Fragments arranged by size: 4545 1444 453 427 163 75 73 BamHI G'GATC_C Cuts at: 2323 4293 4510 4877 2323 Size: 1970 217 367 4626 Fragments arranged by size: 4626 1970 367 217 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2150 2216 2466 2741 2773 Size: 325 217 627 363 66 250 275 32 Cuts at: 2773 2784 2804 3232 3397 4014 4501 5956 618 Size: 11 20 428 165 617 487 1455 1842 Fragments arranged by size: 1842 1455 627 617 487 428 363 325 275 250 217 165 66 32 20 11 BanII G_rGCy'C Cuts at: 1391 2285 3131 3746 4499 1391 Size: 894 846 615 753 4072 Fragments arranged by size: 4072 894 846 753 615 BbeI G_GCGC'C Cuts at: 2745 3401 2745 Size: 656 6524 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6209 BbvCI CC'TCA_GC Cuts at: 3179 3179 Size: 7180 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5371 5405 1903 Size: 34 3434 34 3678 Fragments arranged by size: 3678 3434 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3693 5072 6282 3693 Size: 1379 1210 4591 Fragments arranged by size: 4591 1379 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7180 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5791 136 Size: 107 122 71 631 605 4119 1525 Fragments arranged by size: 4119 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7175 7175 Size: 7180 BlpI GC'TnA_GC Cuts at: 2109 2592 2870 4597 2109 Size: 483 278 1727 4692 Fragments arranged by size: 4692 1727 483 278 Bme1580I G_kGCm'C Cuts at: 1790 2219 3206 3355 3860 4384 5241 6397 1790 Size: 429 987 149 505 524 857 1156 2573 Fragments arranged by size: 2573 1156 987 857 524 505 429 149 BmgBI CAC'GTC Cuts at: 1318 3999 1318 Size: 2681 4499 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4911 5869 442 Size: 416 53 83 399 24 3494 958 1753 Fragments arranged by size: 3494 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7180 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3556 3556 Size: 7180 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2438 2810 3551 5840 1239 Size: 1199 372 741 2289 2579 Fragments arranged by size: 2579 2289 1199 741 372 Bpu10I CC'TnA_GC Cuts at: 2435 3125 3179 3207 3219 3745 2435 Size: 690 54 28 12 526 5870 Fragments arranged by size: 5870 690 526 54 28 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3227 5305 6170 6468 3227 Size: 2078 865 298 3939 Fragments arranged by size: 3939 2078 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3154 5843 882 Size: 617 1655 2689 2219 Fragments arranged by size: 2689 2219 1655 617 BsaAI yAC'GTr Cuts at: 493 1204 2707 3670 4971 493 Size: 711 1503 963 1301 2702 Fragments arranged by size: 2702 1503 1301 963 711 BsaBI GATnn'nnATC Cuts at: 2127 2328 7174 2127 Size: 201 4846 2133 Fragments arranged by size: 4846 2133 201 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2742 2889 3159 3398 Size: 53 83 186 936 1209 147 270 239 Cuts at: 3398 3420 3521 4987 5369 6004 275 Size: 22 101 1466 382 635 1451 Fragments arranged by size: 1466 1451 1209 936 635 382 270 239 186 147 101 83 53 22 BsaWI w'CCGG_w Cuts at: 2610 4275 5613 6011 6285 6432 6843 2610 Size: 1665 1338 398 274 147 411 2947 Fragments arranged by size: 2947 1665 1338 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6955 Fragments arranged by size: 6955 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2169 2435 2438 2807 2918 3142 3532 3739 Size: 983 266 3 369 111 224 390 207 Cuts at: 3739 4446 1186 Size: 707 3920 Fragments arranged by size: 3920 983 707 390 369 266 224 207 111 3 BseYI C'CCAG_C Cuts at: 2730 3130 3167 3304 3663 6383 6879 2730 Size: 400 37 137 359 2720 496 3031 Fragments arranged by size: 3031 2720 496 400 359 137 37 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2528 2669 1163 Size: 573 792 141 5674 Fragments arranged by size: 5674 792 573 141 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2476 4630 5391 6419 1540 Size: 275 186 475 2154 761 1028 2301 Fragments arranged by size: 2301 2154 1028 761 475 275 186 BsiHKAI G_wGCw'C Cuts at: 3746 4384 5241 5326 6397 3746 Size: 638 857 85 1071 4529 Fragments arranged by size: 4529 1071 857 638 85 BsmI GAATG_Cn' Cuts at: 4288 4691 4784 4288 Size: 403 93 6684 Fragments arranged by size: 6684 403 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3261 3261 Size: 7180 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2219 2285 3131 3206 3355 3746 3860 Size: 399 429 66 846 75 149 391 114 Cuts at: 3860 4384 4499 5241 5326 6397 1391 Size: 524 115 742 85 1071 2174 Fragments arranged by size: 2174 1071 846 742 524 429 399 391 149 115 114 85 75 66 BspEI T'CCGG_A Cuts at: 6843 6843 Size: 7180 BspHI T'CATG_A Cuts at: 3954 5069 6799 3954 Size: 1115 1730 4335 Fragments arranged by size: 4335 1730 1115 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2085 2457 3036 3478 4292 4535 844 Size: 1241 372 579 442 814 243 3489 Fragments arranged by size: 3489 1241 814 579 442 372 243 BsrBI CCG'CTC Cuts at: 1394 1823 3875 5067 1394 Size: 429 2052 1192 3507 Fragments arranged by size: 3507 2052 1192 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3615 3828 5675 5849 65 Size: 1183 2367 213 1847 174 1396 Fragments arranged by size: 2367 1847 1396 1183 213 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2044 2295 2718 5824 6011 1700 Size: 110 234 251 423 3106 187 2869 Fragments arranged by size: 3106 2869 423 251 234 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7180 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7180 BssSI C'ACGA_G Cuts at: 5240 6252 5240 Size: 1012 6168 BstAPI GCAn_nnn'nTGC Cuts at: 794 2911 794 Size: 2117 5063 BstBI TT'CG_AA Cuts at: 5984 5984 Size: 7180 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7180 BstXI CCAn_nnnn'nTGG Cuts at: 2738 2738 Size: 7180 BstYI r'GATC_y Cuts at: 1972 2323 4293 4510 4877 5262 5279 6731 6817 Size: 351 1970 217 367 385 17 1452 86 Cuts at: 6817 6829 7175 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1970 1452 385 367 351 346 217 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2076 2159 2240 2285 2470 2880 Size: 552 939 72 83 81 45 185 410 Cuts at: 2880 3924 513 Size: 1044 3769 Fragments arranged by size: 3769 1044 939 552 410 185 83 81 72 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2099 3643 487 Size: 683 56 873 1544 4024 Fragments arranged by size: 4024 1544 873 683 56 BtsI GCAGTG_nn' Cuts at: 3141 3503 3840 4127 4696 5490 5510 3141 Size: 362 337 287 569 794 20 4811 Fragments arranged by size: 4811 794 569 362 337 287 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7180 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7145 DraI TTT'AAA Cuts at: 4484 4484 Size: 7180 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3653 6187 809 Size: 507 2337 2534 1802 Fragments arranged by size: 2534 2337 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2473 2859 3819 Size: 54 1425 50 275 18 643 386 960 Cuts at: 3819 3876 4254 4627 5516 7145 8 Size: 57 378 373 889 1629 43 Fragments arranged by size: 1629 1425 960 889 643 386 378 373 275 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2473 4627 1537 Size: 275 661 2154 4090 Fragments arranged by size: 4090 2154 661 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2983 3711 5110 1353 Size: 1630 728 1399 3423 Fragments arranged by size: 3423 1630 1399 728 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5767 6141 6287 387 Size: 1064 138 4178 374 146 1280 Fragments arranged by size: 4178 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3744 3744 Size: 7180 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2188 2302 2426 2438 2810 3532 3551 Size: 393 556 114 124 12 372 722 19 Cuts at: 3551 3735 3935 4048 4076 5243 5840 6627 6895 Size: 184 200 113 28 1167 597 787 268 Cuts at: 6895 1239 Size: 1524 Fragments arranged by size: 1524 1167 787 722 597 556 393 372 268 200 184 124 114 113 28 19 12 EcoNI CCTnn'n_nnAGG Cuts at: 2840 3547 2840 Size: 707 6473 EcoO109I rG'GnC_Cy Cuts at: 2097 2174 2190 2391 2446 3404 3931 2097 Size: 77 16 201 55 958 527 5346 Fragments arranged by size: 5346 958 527 201 77 55 16 EcoRI G'AATT_C Cuts at: 4489 4867 4489 Size: 378 6802 FspI TGC'GCA Cuts at: 2453 5686 2453 Size: 3233 3947 HaeII r_GCGC'y Cuts at: 1678 2745 3179 3401 4162 6327 1678 Size: 1067 434 222 761 2165 2531 Fragments arranged by size: 2531 2165 1067 761 434 222 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2528 2560 2761 2793 3551 Size: 32 135 32 956 32 201 32 758 Cuts at: 3551 3583 5821 5853 5895 5927 1373 Size: 32 2238 32 42 32 2626 Fragments arranged by size: 2626 2238 956 758 201 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3197 3424 4524 4770 669 Size: 988 281 1259 227 1100 246 3079 Fragments arranged by size: 3079 1259 1100 988 281 246 227 HindIII A'AGCT_T Cuts at: 748 748 Size: 7180 HpaI GTT'AAC Cuts at: 4770 4770 Size: 7180 KasI G'GCGC_C Cuts at: 2741 3397 2741 Size: 656 6524 KpnI G_GTAC'C Cuts at: 2470 4505 2470 Size: 2035 5145 MfeI C'AATT_G Cuts at: 4779 4779 Size: 7180 MluI A'CGCG_T Cuts at: 7056 7056 Size: 7180 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6294 6478 1305 Size: 4989 184 2007 Fragments arranged by size: 4989 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2861 3821 10 Size: 54 1425 343 1029 960 3369 Fragments arranged by size: 3369 1425 1029 960 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2045 3737 3833 3899 5138 5497 5656 Size: 648 879 1692 96 66 1239 359 159 Cuts at: 5656 518 Size: 2042 Fragments arranged by size: 2042 1692 1239 879 648 359 159 96 66 MspA1I CmG'CkG Cuts at: 1606 3784 4054 4596 5273 6421 6666 1606 Size: 2178 270 542 677 1148 245 2120 Fragments arranged by size: 2178 2120 1148 677 542 270 245 NaeI GCC'GGC Cuts at: 2720 2720 Size: 7180 NarI GG'CG_CC Cuts at: 2742 3398 2742 Size: 656 6524 NcoI C'CATG_G Cuts at: 513 1065 2004 2076 2285 2880 3924 513 Size: 552 939 72 209 595 1044 3769 Fragments arranged by size: 3769 1044 939 595 552 209 72 NdeI CA'TA_TG Cuts at: 387 387 Size: 7180 NgoMIV G'CCGG_C Cuts at: 2718 2718 Size: 7180 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7180 NotI GC'GGCC_GC Cuts at: 4627 4627 Size: 7180 NspI r_CATG'y Cuts at: 1132 1229 2044 2980 4101 4192 4538 6083 1132 Size: 97 815 936 1121 91 346 1545 2229 Fragments arranged by size: 2229 1545 1121 936 815 346 97 91 PasI CC'CwG_GG Cuts at: 1348 2552 2916 3355 1348 Size: 1204 364 439 5173 Fragments arranged by size: 5173 1204 439 364 PciI A'CATG_T Cuts at: 4097 4188 6079 4097 Size: 91 1891 5198 Fragments arranged by size: 5198 1891 91 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7180 PfoI T'CCnGG_A Cuts at: 2274 3528 2274 Size: 1254 5926 PmeI GTTT'AAAC Cuts at: 4484 4484 Size: 7180 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3557 3589 5249 5281 6762 6794 3557 Size: 32 1660 32 1481 32 3943 Fragments arranged by size: 3943 1660 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2097 2174 2190 2391 2446 3404 3931 2097 Size: 77 16 201 55 958 527 5346 Fragments arranged by size: 5346 958 527 201 77 55 16 PshAI GACnn'nnGTC Cuts at: 1613 2205 2491 1613 Size: 592 286 6302 Fragments arranged by size: 6302 592 286 PsiI TTA'TAA Cuts at: 4750 4750 Size: 7180 PstI C_TGCA'G Cuts at: 830 2014 4267 4532 830 Size: 1184 2253 265 3478 Fragments arranged by size: 3478 2253 1184 265 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7180 PvuII CAG'CTG Cuts at: 3784 4054 3784 Size: 270 6910 SacI G_AGCT'C Cuts at: 3746 3746 Size: 7180 SalI G'TCGA_C Cuts at: 1936 4522 1936 Size: 2586 4594 SapI GCTCTTCn'nnn_ Cuts at: 2983 2983 Size: 7180 SbfI CC_TGCA'GG Cuts at: 4532 4532 Size: 7180 ScaI AGT'ACT Cuts at: 5428 7140 5428 Size: 1712 5468 SfcI C'TryA_G Cuts at: 826 1045 2010 2638 4263 4528 5663 6344 6535 Size: 219 965 628 1625 265 1135 681 191 Cuts at: 6535 826 Size: 1471 Fragments arranged by size: 1625 1471 1135 965 681 628 265 219 191 SfoI GGC'GCC Cuts at: 2743 3399 2743 Size: 656 6524 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7180 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7180 SmaI CCC'GGG Cuts at: 1872 2825 4507 1872 Size: 953 1682 4545 Fragments arranged by size: 4545 1682 953 SmlI C'TyrA_G Cuts at: 820 1017 1943 3242 5284 6185 6447 820 Size: 197 926 1299 2042 901 262 1553 Fragments arranged by size: 2042 1553 1299 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4971 493 Size: 4478 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7180 SphI G_CATG'C Cuts at: 1132 4538 1132 Size: 3406 3774 SspI AAT'ATT Cuts at: 5 52 5104 5 Size: 47 5052 2081 Fragments arranged by size: 5052 2081 47 StuI AGG'CCT Cuts at: 1517 2636 3750 1517 Size: 1119 1114 4947 Fragments arranged by size: 4947 1119 1114 StyI C'CwwG_G Cuts at: 513 1065 2004 2076 2285 2367 2841 2880 3461 Size: 552 939 72 209 82 474 39 581 Cuts at: 3461 3651 3924 4619 513 Size: 190 273 695 3074 Fragments arranged by size: 3074 939 695 581 552 474 273 209 190 82 72 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2873 3885 4040 5207 5224 5377 5562 Size: 1255 1293 1012 155 1167 17 153 185 Cuts at: 5562 6007 325 Size: 445 1498 Fragments arranged by size: 1498 1293 1255 1167 1012 445 185 155 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4020 5426 7138 Size: 274 80 33 51 1443 2043 1406 1712 Cuts at: 7138 96 Size: 138 Fragments arranged by size: 2043 1712 1443 1406 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2219 2251 4087 4119 7140 7172 2219 Size: 32 1836 32 3021 32 2227 Fragments arranged by size: 3021 2227 1836 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7180 XbaI T'CTAG_A Cuts at: 4516 4516 Size: 7180 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2689 2689 Size: 7180 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7180 XmaI C'CCGG_G Cuts at: 1870 2823 4505 1870 Size: 953 1682 4545 Fragments arranged by size: 4545 1682 953 XmnI GAAnn'nnTTC Cuts at: 4061 5309 4061 Size: 1248 5932 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4988 276 Size: 53 83 186 936 3454 2468 Fragments arranged by size: 3454 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI