(Linear) (Six-base) MAPSORT of: KIEE4232.seq Check: 9393 from: 1 to: 2475 KIEE4232.orf With 182 enzymes: SgfI * March 22, 2012 17:37 .. Acc65I G'GTAC_C Cuts at: 0 461 2475 Size: 461 2014 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 183 297 421 1527 1730 1930 2043 2071 Size: 183 114 124 1106 203 200 113 28 Cuts at: 2071 2475 Size: 404 Fragments arranged by size: 1106 404 203 200 183 124 114 113 28 AflIII A'CryG_T Cuts at: 0 2092 2183 2475 Size: 2092 91 292 Fragments arranged by size: 2092 292 91 AhdI GACnn_n'nnGTC Cuts at: 0 537 2475 Size: 537 1938 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2086 2475 Size: 2086 389 ApaLI G'TGCA_C Cuts at: 0 2375 2475 Size: 2375 100 ApoI r'AATT_y Cuts at: 0 13 903 2341 2407 2475 Size: 13 890 1438 66 68 Fragments arranged by size: 1438 890 68 66 13 AvaI C'yCGr_G Cuts at: 0 391 818 893 1056 2475 Size: 391 427 75 163 1419 Fragments arranged by size: 1419 427 391 163 75 BamHI G'GATC_C Cuts at: 0 318 2288 2475 Size: 318 1970 187 Fragments arranged by size: 1970 318 187 BanI G'GyrC_C Cuts at: 0 145 211 461 736 768 779 799 1227 Size: 145 66 250 275 32 11 20 428 Cuts at: 1227 1392 2009 2475 Size: 165 617 466 Fragments arranged by size: 617 466 428 275 250 165 145 66 32 20 11 BanII G_rGCy'C Cuts at: 0 280 1126 1741 2475 Size: 280 846 615 734 Fragments arranged by size: 846 734 615 280 BbeI G_GCGC'C Cuts at: 0 740 1396 2475 Size: 740 656 1079 Fragments arranged by size: 1079 740 656 BbvCI CC'TCA_GC Cuts at: 0 1174 2475 Size: 1174 1301 BciVI GTATCCnnnnn_n' Cuts at: 0 1688 2475 Size: 1688 787 BlpI GC'TnA_GC Cuts at: 0 104 587 865 2475 Size: 104 483 278 1610 Fragments arranged by size: 1610 483 278 104 Bme1580I G_kGCm'C Cuts at: 0 214 1201 1350 1855 2379 2475 Size: 214 987 149 505 524 96 Fragments arranged by size: 987 524 505 214 149 96 BmgBI CAC'GTC Cuts at: 0 1994 2475 Size: 1994 481 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1551 2475 Size: 1551 924 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 433 805 1546 2475 Size: 433 372 741 929 Fragments arranged by size: 929 741 433 372 Bpu10I CC'TnA_GC Cuts at: 0 430 1120 1174 1202 1214 1740 2475 Size: 430 690 54 28 12 526 735 Fragments arranged by size: 735 690 526 430 54 28 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1222 2475 Size: 1222 1253 BsaI GGTCTCn'nnnn_ Cuts at: 0 1149 2475 Size: 1149 1326 BsaAI yAC'GTr Cuts at: 0 702 1665 2475 Size: 702 963 810 Fragments arranged by size: 963 810 702 BsaBI GATnn'nnATC Cuts at: 0 122 323 2475 Size: 122 201 2152 Fragments arranged by size: 2152 201 122 BsaHI Gr'CG_yC Cuts at: 0 737 884 1154 1393 1415 1516 2475 Size: 737 147 270 239 22 101 959 Fragments arranged by size: 959 737 270 239 147 101 22 BsaWI w'CCGG_w Cuts at: 0 605 2270 2475 Size: 605 1665 205 Fragments arranged by size: 1665 605 205 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 164 430 433 802 913 1137 1527 1734 Size: 164 266 3 369 111 224 390 207 Cuts at: 1734 2441 2475 Size: 707 34 Fragments arranged by size: 707 390 369 266 224 207 164 111 34 3 BseYI C'CCAG_C Cuts at: 0 725 1125 1162 1299 1658 2475 Size: 725 400 37 137 359 817 Fragments arranged by size: 817 725 400 359 137 37 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 523 664 2475 Size: 523 141 1811 Fragments arranged by size: 1811 523 141 BsiEI CG_ry'CG Cuts at: 0 471 2475 Size: 471 2004 BsiHKAI G_wGCw'C Cuts at: 0 1741 2379 2475 Size: 1741 638 96 Fragments arranged by size: 1741 638 96 BsmI GAATG_Cn' Cuts at: 0 2283 2475 Size: 2283 192 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1256 2475 Size: 1256 1219 Bsp1286I G_dGCh'C Cuts at: 0 214 280 1126 1201 1350 1741 1855 2379 Size: 214 66 846 75 149 391 114 524 Cuts at: 2379 2475 Size: 96 Fragments arranged by size: 846 524 391 214 149 114 96 75 66 BspHI T'CATG_A Cuts at: 0 1949 2475 Size: 1949 526 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 80 452 1031 1473 2287 2475 Size: 80 372 579 442 814 188 Fragments arranged by size: 814 579 442 372 188 80 BsrBI CCG'CTC Cuts at: 0 1870 2475 Size: 1870 605 BsrDI GCAATG_nn' Cuts at: 0 1610 1823 2475 Size: 1610 213 652 Fragments arranged by size: 1610 652 213 BsrFI r'CCGG_y Cuts at: 0 39 290 713 2475 Size: 39 251 423 1762 Fragments arranged by size: 1762 423 251 39 BstAPI GCAn_nnn'nTGC Cuts at: 0 906 2475 Size: 906 1569 BstXI CCAn_nnnn'nTGG Cuts at: 0 733 2475 Size: 733 1742 BstYI r'GATC_y Cuts at: 0 318 2288 2475 Size: 318 1970 187 Fragments arranged by size: 1970 318 187 BtgI C'CryG_G Cuts at: 0 71 154 235 280 465 875 1919 2475 Size: 71 83 81 45 185 410 1044 556 Fragments arranged by size: 1044 556 410 185 83 81 71 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 94 1638 2475 Size: 94 1544 837 Fragments arranged by size: 1544 837 94 BtsI GCAGTG_nn' Cuts at: 0 1136 1498 1835 2122 2475 Size: 1136 362 337 287 353 Fragments arranged by size: 1136 362 353 337 287 DrdI GACnn_nn'nnGTC Cuts at: 0 1648 2475 Size: 1648 827 EaeI y'GGCC_r Cuts at: 0 468 854 1814 1871 2249 2475 Size: 468 386 960 57 378 226 Fragments arranged by size: 960 468 386 378 226 57 EagI C'GGCC_G Cuts at: 0 468 2475 Size: 468 2007 EarI CTCTTCn'nnn_ Cuts at: 0 978 1706 2475 Size: 978 728 769 Fragments arranged by size: 978 769 728 EcoICRI GAG'CTC Cuts at: 0 1739 2475 Size: 1739 736 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 183 297 421 433 805 1527 1546 1730 Size: 183 114 124 12 372 722 19 184 Cuts at: 1730 1930 2043 2071 2475 Size: 200 113 28 404 Fragments arranged by size: 722 404 372 200 184 183 124 114 113 28 19 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 835 1542 2475 Size: 835 707 933 Fragments arranged by size: 933 835 707 EcoO109I rG'GnC_Cy Cuts at: 0 92 169 185 386 441 1399 1926 2475 Size: 92 77 16 201 55 958 527 549 Fragments arranged by size: 958 549 527 201 92 77 55 16 FspI TGC'GCA Cuts at: 0 448 2475 Size: 448 2027 HaeII r_GCGC'y Cuts at: 0 740 1174 1396 2157 2475 Size: 740 434 222 761 318 Fragments arranged by size: 761 740 434 318 222 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 523 555 756 788 1546 1578 2475 Size: 523 32 201 32 758 32 897 Fragments arranged by size: 897 758 523 201 32 32 32 HincII GTy'rAC Cuts at: 0 1192 1419 2475 Size: 1192 227 1056 Fragments arranged by size: 1192 1056 227 KasI G'GCGC_C Cuts at: 0 736 1392 2475 Size: 736 656 1083 Fragments arranged by size: 1083 736 656 KpnI G_GTAC'C Cuts at: 0 465 2475 Size: 465 2010 MscI TGG'CCA Cuts at: 0 856 1816 2475 Size: 856 960 659 Fragments arranged by size: 960 856 659 MslI CAynn'nnrTG Cuts at: 0 40 1732 1828 1894 2475 Size: 40 1692 96 66 581 Fragments arranged by size: 1692 581 96 66 40 MspA1I CmG'CkG Cuts at: 0 1779 2049 2475 Size: 1779 270 426 Fragments arranged by size: 1779 426 270 NaeI GCC'GGC Cuts at: 0 715 2475 Size: 715 1760 NarI GG'CG_CC Cuts at: 0 737 1393 2475 Size: 737 656 1082 Fragments arranged by size: 1082 737 656 NcoI C'CATG_G Cuts at: 0 71 280 875 1919 2475 Size: 71 209 595 1044 556 Fragments arranged by size: 1044 595 556 209 71 NgoMIV G'CCGG_C Cuts at: 0 713 2475 Size: 713 1762 NspI r_CATG'y Cuts at: 0 39 975 2096 2187 2475 Size: 39 936 1121 91 288 Fragments arranged by size: 1121 936 288 91 39 PasI CC'CwG_GG Cuts at: 0 547 911 1350 2475 Size: 547 364 439 1125 Fragments arranged by size: 1125 547 439 364 PciI A'CATG_T Cuts at: 0 2092 2183 2475 Size: 2092 91 292 Fragments arranged by size: 2092 292 91 PfoI T'CCnGG_A Cuts at: 0 269 1523 2475 Size: 269 1254 952 Fragments arranged by size: 1254 952 269 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1552 1584 2475 Size: 1552 32 891 Fragments arranged by size: 1552 891 32 PpuMI rG'GwC_Cy Cuts at: 0 92 169 185 386 441 1399 1926 2475 Size: 92 77 16 201 55 958 527 549 Fragments arranged by size: 958 549 527 201 92 77 55 16 PshAI GACnn'nnGTC Cuts at: 0 200 486 2475 Size: 200 286 1989 Fragments arranged by size: 1989 286 200 PstI C_TGCA'G Cuts at: 0 9 2262 2475 Size: 9 2253 213 Fragments arranged by size: 2253 213 9 PvuII CAG'CTG Cuts at: 0 1779 2049 2475 Size: 1779 270 426 Fragments arranged by size: 1779 426 270 SacI G_AGCT'C Cuts at: 0 1741 2475 Size: 1741 734 SapI GCTCTTCn'nnn_ Cuts at: 0 978 2475 Size: 978 1497 SfcI C'TryA_G Cuts at: 0 5 633 2258 2475 Size: 5 628 1625 217 Fragments arranged by size: 1625 628 217 5 SfoI GGC'GCC Cuts at: 0 738 1394 2475 Size: 738 656 1081 Fragments arranged by size: 1081 738 656 SmaI CCC'GGG Cuts at: 0 820 2475 Size: 820 1655 SmlI C'TyrA_G Cuts at: 0 1237 2475 Size: 1237 1238 StuI AGG'CCT Cuts at: 0 631 1745 2475 Size: 631 1114 730 Fragments arranged by size: 1114 730 631 StyI C'CwwG_G Cuts at: 0 71 280 362 836 875 1456 1646 1919 Size: 71 209 82 474 39 581 190 273 Cuts at: 1919 2475 Size: 556 Fragments arranged by size: 581 556 474 273 209 190 82 71 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 868 1880 2035 2475 Size: 868 1012 155 440 Fragments arranged by size: 1012 868 440 155 TatI w'GTAC_w Cuts at: 0 2015 2475 Size: 2015 460 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 214 246 2082 2114 2475 Size: 214 32 1836 32 361 Fragments arranged by size: 1836 361 214 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 684 2475 Size: 684 1791 XmaI C'CCGG_G Cuts at: 0 818 2475 Size: 818 1657 XmnI GAAnn'nnTTC Cuts at: 0 2056 2475 Size: 2056 419 Enzymes that do cut: Acc65I AcuI AflIII AhdI AlfI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbvCI BciVI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI BtgI BtgZI BtsI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII KasI KpnI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI PpiI PpuMI PshAI PstI PvuII SacI SapI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AgeI AleI AloI AlwNI ApaI AscI AseI AsiSI AvrII BaeI BbsI BcgI BclI BfrBI BglI BglII BmrI BmtI BsaXI BsiWI BspEI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII EciI EcoRI EcoRV FalI FseI FspAI HindIII HpaI MfeI MluI MmeI NdeI NheI NotI NruI NsiI PacI PflMI PmeI PmlI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI ZraI