Restriction map for
pFN21AE1164
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI AlwI AlwNI AocI
Aor51HI ApaI AscI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BglI BlnI BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MroI MspA1I
MspI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 782 GTCTAC GT^[AC][GT]AC
AccII 1448 CGCG CG^CG
AflII 496 CTTAAG C^TTAAG
AflIII 1447 ACGCGT A^C[GA][CT]GT
Alw44I 491 GTGCAC G^TGCAC
ApaLI 491 GTGCAC G^TGCAC
AseI 886 ATTAAT AT^TAAT
AsnI 886 ATTAAT AT^TAAT
BalI 375 TGGCCA TGG^CCA
BfrI 496 CTTAAG C^TTAAG
BglII 1459 AGATCT A^GATCT
BlpI 1252 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1252 GCTGAGC GC^T[ACGT]AGC
BsaI 44 GGTCTC GGTCTC(1/5)
Bsp1407I 313 TGTACA T^GTACA
BsrGI 313 TGTACA T^GTACA
BstUI 1448 CGCG CG^CG
BstYI 1459 AGATCT [GA]^GATC[CT]
CelII 1252 GCTGAGC GC^T[ACGT]AGC
DraI 1114 TTTAAA TTT^AAA
DraII 621 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 84 GACCAAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 375 TGGCCA [CT]^GGCC[GA]
EcoO109I 621 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1040 GAATTC G^AATTC
EcoRV 383 GATATC GAT^ATC
EcoT14I 378 CCAAGG C^C[AT][AT]GG
HphI 1722 GGTGA GGTGA(8/7)
MflI 1459 AGATCT [GA]^GATC[CT]
MluI 1447 ACGCGT A^CGCGT
MluNI 375 TGGCCA TGG^CCA
MscI 375 TGGCCA TGG^CCA
MvnI 1448 CGCG CG^CG
NlaIV 286 GGAGCC GG[ACGT]^[ACGT]CC
PshBI 886 ATTAAT AT^TAAT
SapI 1514 GAAGAGC GCTCTTC(1/4)
SspBI 313 TGTACA T^GTACA
StyI 378 CCAAGG C^C[AT][AT]GG
TaqI 57 TCGA T^CGA
TthHB8I 57 TCGA T^CGA
XhoII 1459 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 42 GCGG CCGC(-3/-1)
1488 GCGG
AfaI 314 GTAC GT^AC
1451 GTAC
AspHI 491 GTGCAC G[AT]GC[AT]^C
1517 GAGCAC
BbsI 241 GAAGAC GAAGAC(2/6)
1505 GTCTTC
BmyI 491 GTGCAC G[AGT]GC[ACT]^C
1517 GAGCAC
BpuAI 241 GAAGAC GAAGAC(2/6)
1505 GTCTTC
BsgI 1334 CTGCAC GTGCAG(16/14)
1741 GTGCAG
BsiHKAI 491 GTGCAC G[AT]GC[AT]^C
1517 GAGCAC
BsiYI 564 CCTTGAATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1704 CCGAGTTTTGG
BslI 564 CCTTGAATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1704 CCGAGTTTTGG
Bsp1286I 491 GTGCAC G[AGT]GC[ACT]^C
1517 GAGCAC
BstXI 347 CCAATGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
787 CCATTACAGTGG
DpnII 1460 GATC ^GATC
1618 GATC
MboI 1460 GATC ^GATC
1618 GATC
NdeII 1460 GATC ^GATC
1618 GATC
PstI 753 CTGCAG CTGCA^G
1673 CTGCAG
RsaI 314 GTAC GT^AC
1451 GTAC
Sau3AI 1460 GATC ^GATC
1618 GATC
SmlI 496 CTTAAG C^T[CT][GA]AG
868 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AcsI AluI ApoI AvaII BbvI
BfaI BpmI BsaJI BsmAI BspHI
BspMI BsrDI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI EarI
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI Ksp632I MaeI MaeII MaeIII
MboII MnlI MseI MslI MvaI
MwoI NlaIII NspI PleI RcaI
Sau96I ScrFI SfaNI SfcI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI