(Circular) (Six-base) MAPSORT of: pFN21AE1164.seq Check: 992 from: 1 to: 6484 pFN21AE1164 6484 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4294 278 Size: 53 83 186 936 2758 2468 Fragments arranged by size: 2758 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2788 3827 1937 Size: 851 1039 4594 Fragments arranged by size: 4594 1039 851 Acc65I G'GTAC_C Cuts at: 3805 3805 Size: 6484 AclI AA'CG_TT Cuts at: 1563 4611 4984 1563 Size: 3048 373 3063 Fragments arranged by size: 3063 3048 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3120 4547 5931 6199 1632 Size: 1488 1427 1384 268 1917 Fragments arranged by size: 1917 1488 1427 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2501 820 Size: 197 1484 4803 Fragments arranged by size: 4803 1484 197 AflIII A'CryG_T Cuts at: 1203 3452 5383 6360 1203 Size: 2249 1931 977 1327 Fragments arranged by size: 2249 1931 1327 977 AgeI A'CCGG_T Cuts at: 5315 5315 Size: 6484 AhdI GACnn_n'nnGTC Cuts at: 5213 5213 Size: 6484 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6484 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2836 1184 Size: 1652 4832 AlwNI CAG_nnn'CTG Cuts at: 4227 5799 4227 Size: 1572 4912 ApaLI G'TGCA_C Cuts at: 2496 4541 5697 2496 Size: 2045 1156 3283 Fragments arranged by size: 3283 2045 1156 ApoI r'AATT_y Cuts at: 1493 2138 3045 3116 3793 4019 4171 6280 1493 Size: 645 907 71 677 226 152 2109 1697 Fragments arranged by size: 2109 1697 907 677 645 226 152 71 AseI AT'TA_AT Cuts at: 160 1032 2892 5038 160 Size: 872 1860 2146 1606 Fragments arranged by size: 2146 1860 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 AvaI C'yCGr_G Cuts at: 1870 1943 3809 1870 Size: 73 1866 4545 Fragments arranged by size: 4545 1866 73 BamHI G'GATC_C Cuts at: 3814 4181 3814 Size: 367 6117 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3805 5260 618 Size: 325 217 627 2018 1455 1842 Fragments arranged by size: 2018 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3803 1391 Size: 2412 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2253 3503 928 Size: 971 354 1250 3909 Fragments arranged by size: 3909 1250 971 354 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4675 4709 1903 Size: 34 2738 34 3678 Fragments arranged by size: 3678 2738 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4376 5586 4376 Size: 1210 5274 BclI T'GATC_A Cuts at: 1548 1548 Size: 6484 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5095 136 Size: 107 122 71 631 605 3423 1525 Fragments arranged by size: 3423 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3464 6479 3464 Size: 3015 3469 BlpI GC'TnA_GC Cuts at: 3258 3901 3258 Size: 643 5841 Bme1580I G_kGCm'C Cuts at: 1790 2500 4545 5701 1790 Size: 710 2045 1156 2573 Fragments arranged by size: 2573 2045 1156 710 BmgBI CAC'GTC Cuts at: 1318 3722 1318 Size: 2404 4080 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2911 4215 5173 Size: 416 53 83 399 24 1494 1304 958 Cuts at: 5173 442 Size: 1753 Fragments arranged by size: 1753 1494 1304 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6484 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2652 2940 2987 3424 3512 5144 1239 Size: 1413 288 47 437 88 1632 2579 Fragments arranged by size: 2579 1632 1413 437 288 88 47 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2894 4609 5474 5772 2894 Size: 1715 865 298 3606 Fragments arranged by size: 3606 1715 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2055 5147 882 Size: 617 556 3092 2219 Fragments arranged by size: 3092 2219 617 556 BsaAI yAC'GTr Cuts at: 493 1204 4275 493 Size: 711 3071 2702 Fragments arranged by size: 3071 2702 711 BsaBI GATnn'nnATC Cuts at: 6478 6478 Size: 6484 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4291 4673 5308 275 Size: 53 83 186 936 2758 382 635 1451 Fragments arranged by size: 2758 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4917 5315 5589 5736 6147 4917 Size: 398 274 147 411 5254 Fragments arranged by size: 5254 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6259 Fragments arranged by size: 6259 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6484 BseYI C'CCAG_C Cuts at: 5687 6183 5687 Size: 496 5988 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3324 3767 1163 Size: 573 1588 443 3880 Fragments arranged by size: 3880 1588 573 443 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3934 4695 5723 1540 Size: 275 186 1933 761 1028 2301 Fragments arranged by size: 2301 1933 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2500 3526 4545 4630 5701 2500 Size: 1026 1019 85 1071 3283 Fragments arranged by size: 3283 1071 1026 1019 85 BsmI GAATG_Cn' Cuts at: 3995 4088 3995 Size: 93 6391 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2500 3526 3803 4545 4630 5701 1391 Size: 399 710 1026 277 742 85 1071 2174 Fragments arranged by size: 2174 1071 1026 742 710 399 277 85 BspEI T'CCGG_A Cuts at: 6147 6147 Size: 6484 BspHI T'CATG_A Cuts at: 2535 2683 3112 4373 6103 2535 Size: 148 429 1261 1730 2916 Fragments arranged by size: 2916 1730 1261 429 148 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2015 2573 3671 3839 844 Size: 1171 558 1098 168 3489 Fragments arranged by size: 3489 1171 1098 558 168 BsrBI CCG'CTC Cuts at: 1394 1823 4371 1394 Size: 429 2548 3507 Fragments arranged by size: 3507 2548 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2525 2705 3181 4979 5153 65 Size: 1183 1277 180 476 1798 174 1396 Fragments arranged by size: 1798 1396 1277 1183 476 180 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5128 5315 1700 Size: 110 3318 187 2869 Fragments arranged by size: 3318 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2318 96 Size: 2222 4262 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6484 BssSI C'ACGA_G Cuts at: 4544 5556 4544 Size: 1012 5472 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6484 BstBI TT'CG_AA Cuts at: 5288 5288 Size: 6484 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6484 BstXI CCAn_nnnn'nTGG Cuts at: 2359 2799 2359 Size: 440 6044 BstYI r'GATC_y Cuts at: 1972 3464 3814 4181 4566 4583 6035 6121 6133 Size: 1492 350 367 385 17 1452 86 12 Cuts at: 6133 6479 1972 Size: 346 1977 Fragments arranged by size: 1977 1492 1452 385 367 350 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5932 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5745 Fragments arranged by size: 5745 683 56 BtsI GCAGTG_nn' Cuts at: 2468 2612 2681 4000 4794 4814 2468 Size: 144 69 1319 794 20 4138 Fragments arranged by size: 4138 1319 794 144 69 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6484 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6449 DraI TTT'AAA Cuts at: 3121 3788 3121 Size: 667 5817 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2095 5491 809 Size: 507 779 3396 1802 Fragments arranged by size: 3396 1802 779 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2380 3931 4820 Size: 54 1425 50 275 18 550 1551 889 Cuts at: 4820 6449 8 Size: 1629 43 Fragments arranged by size: 1629 1551 1425 889 550 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3931 1537 Size: 275 2119 4090 Fragments arranged by size: 4090 2119 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2139 2328 3496 3514 4414 1353 Size: 786 189 1168 18 900 3423 Fragments arranged by size: 3423 1168 900 786 189 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5071 5445 5591 387 Size: 1064 138 3482 374 146 1280 Fragments arranged by size: 3482 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2652 2940 2987 3120 3424 3512 4547 Size: 393 1020 288 47 133 304 88 1035 Cuts at: 4547 5144 5931 6199 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1035 1020 787 597 393 304 288 268 133 88 47 EcoO109I rG'GnC_Cy Cuts at: 2627 2627 Size: 6484 EcoRI G'AATT_C Cuts at: 3045 3793 4171 3045 Size: 748 378 5358 Fragments arranged by size: 5358 748 378 EcoRV GAT'ATC Cuts at: 2390 2390 Size: 6484 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2875 2875 Size: 6484 FspI TGC'GCA Cuts at: 4990 4990 Size: 6484 HaeII r_GCGC'y Cuts at: 1678 5631 1678 Size: 3953 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3263 3295 5125 5157 5199 Size: 32 135 32 1691 32 1830 32 42 Cuts at: 5199 5231 1373 Size: 32 2626 Fragments arranged by size: 2626 1830 1691 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3828 4074 669 Size: 988 281 1890 246 3079 Fragments arranged by size: 3079 1890 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6484 HpaI GTT'AAC Cuts at: 4074 4074 Size: 6484 KpnI G_GTAC'C Cuts at: 3809 3809 Size: 6484 MfeI C'AATT_G Cuts at: 4083 4083 Size: 6484 MluI A'CGCG_T Cuts at: 3452 6360 3452 Size: 2908 3576 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5598 5782 1305 Size: 4293 184 2007 Fragments arranged by size: 4293 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2382 10 Size: 54 1425 343 550 4112 Fragments arranged by size: 4112 1425 550 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2797 2818 2996 4442 4801 4960 518 Size: 648 1631 21 178 1446 359 159 2042 Fragments arranged by size: 2042 1631 1446 648 359 178 159 21 MspA1I CmG'CkG Cuts at: 1606 3900 4577 5725 5970 1606 Size: 2294 677 1148 245 2120 Fragments arranged by size: 2294 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5932 NdeI CA'TA_TG Cuts at: 387 387 Size: 6484 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6484 NotI GC'GGCC_GC Cuts at: 3931 3931 Size: 6484 NspI r_CATG'y Cuts at: 1132 1229 2136 2963 3696 3842 5387 1132 Size: 97 907 827 733 146 1545 2229 Fragments arranged by size: 2229 1545 907 827 733 146 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6484 PciI A'CATG_T Cuts at: 5383 5383 Size: 6484 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6484 PmeI GTTT'AAAC Cuts at: 3788 3788 Size: 6484 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4553 4585 6066 6098 4553 Size: 32 1481 32 4939 Fragments arranged by size: 4939 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6484 PsiI TTA'TAA Cuts at: 4054 4054 Size: 6484 PstI C_TGCA'G Cuts at: 830 2762 3682 3836 830 Size: 1932 920 154 3478 Fragments arranged by size: 3478 1932 920 154 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6484 SalI G'TCGA_C Cuts at: 1936 3826 1936 Size: 1890 4594 SapI GCTCTTCn'nnn_ Cuts at: 3514 3514 Size: 6484 SbfI CC_TGCA'GG Cuts at: 3836 3836 Size: 6484 ScaI AGT'ACT Cuts at: 4732 6444 4732 Size: 1712 4772 SfcI C'TryA_G Cuts at: 826 1045 2016 2554 2654 2758 3207 3318 3678 Size: 219 971 538 100 104 449 111 360 Cuts at: 3678 3832 4967 5648 5839 826 Size: 154 1135 681 191 1471 Fragments arranged by size: 1471 1135 971 681 538 449 360 219 191 154 111 104 100 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6484 SmaI CCC'GGG Cuts at: 1872 3811 1872 Size: 1939 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2501 2873 4588 5489 5751 820 Size: 197 926 558 372 1715 901 262 1553 Fragments arranged by size: 1715 1553 926 901 558 372 262 197 SnaBI TAC'GTA Cuts at: 493 4275 493 Size: 3782 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6484 SphI G_CATG'C Cuts at: 1132 3842 1132 Size: 2710 3774 SspI AAT'ATT Cuts at: 5 52 4408 5 Size: 47 4356 2081 Fragments arranged by size: 4356 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6484 StyI C'CwwG_G Cuts at: 513 1065 2383 3923 513 Size: 552 1318 1540 3074 Fragments arranged by size: 3074 1540 1318 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4511 4528 4681 4866 5311 325 Size: 1255 2931 17 153 185 445 1498 Fragments arranged by size: 2931 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2318 4730 6442 Size: 274 80 33 51 1443 341 2412 1712 Cuts at: 6442 96 Size: 138 Fragments arranged by size: 2412 1712 1443 341 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6444 6476 6444 Size: 32 6452 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6484 XbaI T'CTAG_A Cuts at: 3820 3820 Size: 6484 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6484 XmaI C'CCGG_G Cuts at: 1870 3809 1870 Size: 1939 4545 XmnI GAAnn'nnTTC Cuts at: 4613 4613 Size: 6484 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4292 276 Size: 53 83 186 936 2758 2468 Fragments arranged by size: 2758 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI