Restriction map for
pFN21AE9395
(Circular) (Six-base) MAPSORT of: pFN21AE9395.seq Check: 6791 from: 1 to: 6541
pFN21AE9395 6541 bp
With 182 enzymes: SgfI *
January 30, 2014 17:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2263 3061 2263
Size: 798 5743
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4351 278
Size: 53 83 186 936 2815 2468
Fragments arranged by size:
2815 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3884 1937
Size: 1947 4594
Acc65I G'GTAC_C
Cuts at: 3862 3862
Size: 6541
AclI AA'CG_TT
Cuts at: 1563 2284 4668 5041 1563
Size: 721 2384 373 3063
Fragments arranged by size:
3063 2384 721 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3102 3551 4604 5988 6256 1632
Size: 1470 449 1053 1384 268 1917
Fragments arranged by size:
1917 1470 1384 1053 449 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6344
AflIII A'CryG_T
Cuts at: 1203 2864 5440 6417 1203
Size: 1661 2576 977 1327
Fragments arranged by size:
2576 1661 1327 977
AgeI A'CCGG_T
Cuts at: 5372 5372
Size: 6541
AhdI GACnn_n'nnGTC
Cuts at: 5270 5270
Size: 6541
AleI CACnn'nnGTG
Cuts at: 1166 2265 1166
Size: 1099 5442
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6541
AlwNI CAG_nnn'CTG
Cuts at: 4284 5856 4284
Size: 1572 4969
ApaLI G'TGCA_C
Cuts at: 4598 5754 4598
Size: 1156 5385
ApoI r'AATT_y
Cuts at: 1493 2162 2358 2454 2704 2944 3023 3355 3850
Size: 669 196 96 250 240 79 332 495
Cuts at: 3850 4076 4228 6337 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 669 495 332 250 240 226
196 152 96 79
AseI AT'TA_AT
Cuts at: 160 1032 5095 160
Size: 872 4063 1606
Fragments arranged by size:
4063 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6541
AvaI C'yCGr_G
Cuts at: 1870 1943 3866 1870
Size: 73 1923 4545
Fragments arranged by size:
4545 1923 73
BamHI G'GATC_C
Cuts at: 3871 4238 3871
Size: 367 6174
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3862 5317 618
Size: 325 217 627 2075 1455 1842
Fragments arranged by size:
2075 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2053 3320 3860 1391
Size: 662 1267 540 4072
Fragments arranged by size:
4072 1267 662 540
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2019 3143 3798 928
Size: 971 120 1124 655 3671
Fragments arranged by size:
3671 1124 971 655 120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4732 4766 1903
Size: 34 2795 34 3678
Fragments arranged by size:
3678 2795 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4433 5643 4433
Size: 1210 5331
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6541
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5152 136
Size: 107 122 71 631 605 3480 1525
Fragments arranged by size:
3480 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6536 6536
Size: 6541
BlpI GC'TnA_GC
Cuts at: 3958 3958
Size: 6541
Bme1580I G_kGCm'C
Cuts at: 1790 4602 5758 1790
Size: 2812 1156 2573
Fragments arranged by size:
2812 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6541
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4272 5230 442
Size: 416 53 83 399 24 2855 958 1753
Fragments arranged by size:
2855 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6541
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2193 3297 2193
Size: 1104 5437
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2451 2559 3292 5201 1239
Size: 1212 108 733 1909 2579
Fragments arranged by size:
2579 1909 1212 733 108
Bpu10I CC'TnA_GC
Cuts at: 3319 3319
Size: 6541
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3472 4666 5531 5829 3472
Size: 1194 865 298 4184
Fragments arranged by size:
4184 1194 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5204 882
Size: 617 3705 2219
Fragments arranged by size:
3705 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4332 493
Size: 711 3128 2702
Fragments arranged by size:
3128 2702 711
BsaBI GATnn'nnATC
Cuts at: 6535 6535
Size: 6541
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4348 4730 5365 275
Size: 53 83 186 936 2815 382 635 1451
Fragments arranged by size:
2815 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4974 5372 5646 5793 6204 4974
Size: 398 274 147 411 5311
Fragments arranged by size:
5311 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2532 2562 1178
Size: 30 165 30 1129 30 5157
Fragments arranged by size:
5157 1129 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2219 1186
Size: 1033 5508
BseYI C'CCAG_C
Cuts at: 2018 2329 5744 6240 2018
Size: 311 3415 496 2319
Fragments arranged by size:
3415 2319 496 311
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2714 3024 3089 1163
Size: 573 978 310 65 4615
Fragments arranged by size:
4615 978 573 310 65
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2038 3991 4752 5780 1540
Size: 275 186 37 1953 761 1028 2301
Fragments arranged by size:
2301 1953 1028 761 275 186 37
BsiHKAI G_wGCw'C
Cuts at: 4602 4687 5758 4602
Size: 85 1071 5385
Fragments arranged by size:
5385 1071 85
BsmI GAATG_Cn'
Cuts at: 3711 4052 4145 3711
Size: 341 93 6107
Fragments arranged by size:
6107 341 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2053 3320 3860 4602 4687 5758 1391
Size: 399 263 1267 540 742 85 1071 2174
Fragments arranged by size:
2174 1267 1071 742 540 399 263 85
BspEI T'CCGG_A
Cuts at: 6204 6204
Size: 6541
BspHI T'CATG_A
Cuts at: 4430 6160 4430
Size: 1730 4811
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2066 2263 3061 3661 3896 844
Size: 1222 197 798 600 235 3489
Fragments arranged by size:
3489 1222 798 600 235 197
BsrBI CCG'CTC
Cuts at: 1394 1823 4428 1394
Size: 429 2605 3507
Fragments arranged by size:
3507 2605 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5036 5210 65
Size: 1183 3788 174 1396
Fragments arranged by size:
3788 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2033 5185 5372 1700
Size: 110 223 3152 187 2869
Fragments arranged by size:
3152 2869 223 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6541
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6541
BssSI C'ACGA_G
Cuts at: 4601 5613 4601
Size: 1012 5529
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6541
BstBI TT'CG_AA
Cuts at: 3613 5345 3613
Size: 1732 4809
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6541
BstYI r'GATC_y
Cuts at: 1972 3247 3871 4238 4623 4640 6092 6178 6190
Size: 1275 624 367 385 17 1452 86 12
Cuts at: 6190 6536 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1275 624 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5050
Fragments arranged by size:
5050 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5802
Fragments arranged by size:
5802 683 56
BtsI GCAGTG_nn'
Cuts at: 2659 3338 4057 4851 4871 2659
Size: 679 719 794 20 4329
Fragments arranged by size:
4329 794 719 679 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6541
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2284 2319 529
Size: 35 1720 35 4751
Fragments arranged by size:
4751 1720 35 35
DraI TTT'AAA
Cuts at: 3845 3845
Size: 6541
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5548 809
Size: 507 4232 1802
Fragments arranged by size:
4232 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2035 2530 3166
Size: 54 1425 50 275 18 205 495 636
Cuts at: 3166 3988 4877 6506 8
Size: 822 889 1629 43
Fragments arranged by size:
1629 1425 889 822 636 495 275 205
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2035 3988 1537
Size: 275 223 1953 4090
Fragments arranged by size:
4090 1953 275 223
EarI CTCTTCn'nnn_
Cuts at: 1353 2214 2481 3450 3570 4471 1353
Size: 861 267 969 120 901 3423
Fragments arranged by size:
3423 969 901 861 267 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 5128 5502 5648 387
Size: 1064 138 435 3104 374 146 1280
Fragments arranged by size:
3104 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2451 2559 3102 3292 3551 4604 5201
Size: 393 819 108 543 190 259 1053 597
Cuts at: 5201 5988 6256 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1053 819 787 597 543 393 268
259 190 108
EcoRI G'AATT_C
Cuts at: 3850 4228 3850
Size: 378 6163
EcoRV GAT'ATC
Cuts at: 2584 2584
Size: 6541
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2421 3115 3239 2421
Size: 694 124 5723
Fragments arranged by size:
5723 694 124
FspI TGC'GCA
Cuts at: 5047 5047
Size: 6541
HaeII r_GCGC'y
Cuts at: 1678 5688 1678
Size: 4010 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3227 3259 5182 5214 5256
Size: 32 135 32 1655 32 1923 32 42
Cuts at: 5256 5288 1373
Size: 32 2626
Fragments arranged by size:
2626 1923 1655 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3627 3885 4131 669
Size: 988 281 1689 258 246 3079
Fragments arranged by size:
3079 1689 988 281 258 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6541
HpaI GTT'AAC
Cuts at: 3627 4131 3627
Size: 504 6037
KpnI G_GTAC'C
Cuts at: 3866 3866
Size: 6541
MfeI C'AATT_G
Cuts at: 4140 4140
Size: 6541
MluI A'CGCG_T
Cuts at: 6417 6417
Size: 6541
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3067 5655 5839 1305
Size: 1762 2588 184 2007
Fragments arranged by size:
2588 2007 1762 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2532 3168 10
Size: 54 1425 343 700 636 3383
Fragments arranged by size:
3383 1425 700 636 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2265 4499 4858 5017 518
Size: 648 1099 2234 359 159 2042
Fragments arranged by size:
2234 2042 1099 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2058 2144 2728 3828 3957 4634 5782 6027
Size: 452 86 584 1100 129 677 1148 245
Cuts at: 6027 1606
Size: 2120
Fragments arranged by size:
2120 1148 1100 677 584 452 245 129
86
NaeI GCC'GGC
Cuts at: 2035 2035
Size: 6541
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5050
Fragments arranged by size:
5050 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6541
NgoMIV G'CCGG_C
Cuts at: 2033 2033
Size: 6541
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6541
NotI GC'GGCC_GC
Cuts at: 3988 3988
Size: 6541
NspI r_CATG'y
Cuts at: 1132 1229 2868 3899 5444 1132
Size: 97 1639 1031 1545 2229
Fragments arranged by size:
2229 1639 1545 1031 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6541
PciI A'CATG_T
Cuts at: 2864 5440 2864
Size: 2576 3965
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3707 1108
Size: 2599 3942
PmeI GTTT'AAAC
Cuts at: 3845 3845
Size: 6541
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4610 4642 6123 6155 4610
Size: 32 1481 32 4996
Fragments arranged by size:
4996 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6541
PsiI TTA'TAA
Cuts at: 4111 4111
Size: 6541
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2862 2894 2862
Size: 32 6509
PstI C_TGCA'G
Cuts at: 830 3893 830
Size: 3063 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6541
PvuII CAG'CTG
Cuts at: 2144 2728 3828 2144
Size: 584 1100 4857
Fragments arranged by size:
4857 1100 584
SalI G'TCGA_C
Cuts at: 1936 3883 1936
Size: 1947 4594
SapI GCTCTTCn'nnn_
Cuts at: 3450 3450
Size: 6541
SbfI CC_TGCA'GG
Cuts at: 3893 3893
Size: 6541
ScaI AGT'ACT
Cuts at: 2095 4789 6501 2095
Size: 2694 1712 2135
Fragments arranged by size:
2694 2135 1712
SfcI C'TryA_G
Cuts at: 826 1045 2263 3889 5024 5705 5896 826
Size: 219 1218 1626 1135 681 191 1471
Fragments arranged by size:
1626 1471 1218 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6541
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6541
SmaI CCC'GGG
Cuts at: 1872 3868 1872
Size: 1996 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3487 4645 5546 5808 820
Size: 197 926 1544 1158 901 262 1553
Fragments arranged by size:
1553 1544 1158 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4332 493
Size: 3839 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6541
SphI G_CATG'C
Cuts at: 1132 3899 1132
Size: 2767 3774
SspI AAT'ATT
Cuts at: 5 52 2212 2716 3640 4465 5
Size: 47 2160 504 924 825 2081
Fragments arranged by size:
2160 2081 924 825 504 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6541
StyI C'CwwG_G
Cuts at: 513 1065 2004 3980 513
Size: 552 939 1976 3074
Fragments arranged by size:
3074 1976 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3413 4568 4585 4738 4923 5368 325
Size: 1255 1833 1155 17 153 185 445 1498
Fragments arranged by size:
1833 1498 1255 1155 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2093 2548 4787
Size: 274 80 33 51 1443 116 455 2239
Cuts at: 4787 6499 96
Size: 1712 138
Fragments arranged by size:
2239 1712 1443 455 274 138 116 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6501 6533 6501
Size: 32 6509
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6541
XbaI T'CTAG_A
Cuts at: 3754 3877 3754
Size: 123 6418
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6541
XmaI C'CCGG_G
Cuts at: 1870 3866 1870
Size: 1996 4545
XmnI GAAnn'nnTTC
Cuts at: 4670 4670
Size: 6541
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4349 276
Size: 53 83 186 936 2815 2468
Fragments arranged by size:
2815 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI