################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AocI ApaI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII BalI BamHI BbrPI BbvCI BcgI BclI BfrI BglII BlpI Bpu1102I BsaAI BsaBI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrDI BssHII Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII Eam1105I EarI Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI Ksp632I MamI MfeI MflI MluI MluNI MroI MscI MslI MunI NdeI NheI NotI NruI NsiI NspV PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### Acc65I 836 GGTACC G^GTACC AcyI 180 GGCGCC G[GA]^CG[CT]C AlwI 780 GGATC GGATC(4/5) Aor51HI 801 AGCGCT AGC^GCT Asp718I 836 GGTACC G^GTACC AvaI 556 CTCGAG C^[CT]CG[GA]G AvrII 1092 CCTAGG C^CTAGG BbeI 180 GGCGCC GGCGC^C BbsI 417 GTCTTC GAAGAC(2/6) BciVI 490 GTATCC GTATCC(6/5) BfaI 1093 CTAG C^TAG BlnI 1092 CCTAGG C^CTAGG BpuAI 417 GTCTTC GAAGAC(2/6) BsaHI 180 GGCGCC G[GA]^CG[CT]C BsaI 641 GAGACC GGTCTC(1/5) BsaWI 1025 ACCGGA [AT]^CCGG[AT] BseRI 1031 CTCCTC GAGGAG(10/8) BsgI 775 GTGCAG GTGCAG(16/14) BsmAI 641 GAGAC GTCTC(1/5) BsoBI 556 CTCGAG C^[CT]CG[GA]G Bsp1407I 1010 TGTACA T^GTACA BsrFI 288 GCCGGC [GA]^CCGG[CT] BsrGI 1010 TGTACA T^GTACA BssSI 535 CACGAG CACGAG(-5/-1) Cfr10I 288 GCCGGC [GA]^CCGG[CT] DpnII 781 GATC ^GATC DraII 1095 AGGGCCT [GA]G^G[ACGT]CC[CT] Eco47III 801 AGCGCT AGC^GCT EcoO109I 1095 AGGGCCT [GA]G^G[ACGT]CC[CT] Hin1I 180 GGCGCC G[GA]^CG[CT]C KasI 180 GGCGCC G^GCGCC KpnI 836 GGTACC GGTAC^C MaeI 1093 CTAG C^TAG MaeII 415 ACGT A^CGT MaeIII 202 GTCAC ^GT[ACGT]AC MboI 781 GATC ^GATC NaeI 288 GCCGGC GCC^GGC NarI 180 GGCGCC GG^CGCC NcoI 206 CCATGG C^CATGG NdeII 781 GATC ^GATC NgoMIV 288 GCCGGC G^CCGGC PacI 427 TTAATTAA TTAAT^TAA PaeR7I 556 CTCGAG C^TCGAG PflMI 375 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Sau3AI 781 GATC ^GATC SexAI 832 ACCTGGT A^CC[AT]GGT SfiI 390 GGCCGCCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 180 GGCGCC GGC^GCC SmlI 556 CTCGAG C^T[CT][GA]AG SspBI 1010 TGTACA T^GTACA TfiI 598 GATTC G^A[AT]TC Tsp45I 202 GTCAC ^GT[GC]AC Tsp509I 429 AATT ^AATT Van91I 375 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 556 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Alw44I 470 GTGCAC G^TGCAC 532 GTGCAC AlwNI 337 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG 376 CAGCAGCTG ApaLI 470 GTGCAC G^TGCAC 532 GTGCAC BanII 211 GAGCCC G[GA]GC[CT]^C 384 GGGCTC BglI 391 GCCGCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 959 GCCTGCTGGGC BsiEI 290 CGGCCG CG[GA][CT]^CG 389 CGGCCG BsrBI 100 CCGCTC CCGCTC(-3/-3) 754 GAGCGG DdeI 198 CTCAG C^T[ACGT]AG 769 CTGAG DrdI 408 GACTTCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 817 GACACCCAAGTC EagI 290 CGGCCG C^GGCCG 389 CGGCCG EclXI 290 CGGCCG C^GGCCG 389 CGGCCG Eco52I 290 CGGCCG C^GGCCG 389 CGGCCG EcoT14I 206 CCATGG C^C[AT][AT]GG 1092 CCTAGG HgaI 238 GACGC GACGC(5/10) 303 GACGC KspI 119 CCGCGG CCGC^GG 293 CCGCGG MboII 187 TCTTC GAAGA(8/7) 418 TCTTC NspI 466 GCATGT [GA]CATG^[CT] 1019 GCATGT PvuII 379 CAGCTG CAG^CTG 1051 CAGCTG SacII 119 CCGCGG CCGC^GG 293 CCGCGG SfaNI 225 GCATC GCATC(5/9) 249 GCATC StyI 206 CCATGG C^C[AT][AT]GG 1092 CCTAGG ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AccII AciI AfaI AluI AspHI AvaII BanI BbvI BcnI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DsaI EaeI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI PstI RsaI Sau96I ScrFI SfcI TaqI Tru9I TseI TspRI TthHB8I