(Circular) (Six-base) MAPSORT of: pFN21AB7642.seq Check: 1563 from: 1 to: 5866 pFN21AB7642 5866 bp With 182 enzymes: SgfI * March 28, 2012 13:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3676 278 Size: 53 83 186 936 2140 2468 Fragments arranged by size: 2468 2140 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3209 1937 Size: 1272 4594 Acc65I G'GTAC_C Cuts at: 2841 3187 2841 Size: 346 5520 AclI AA'CG_TT Cuts at: 1563 3993 4366 1563 Size: 2430 373 3063 Fragments arranged by size: 3063 2430 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2533 3929 5313 5581 1632 Size: 901 1396 1384 268 1917 Fragments arranged by size: 1917 1396 1384 901 268 AfeI AGC'GCT Cuts at: 2808 2808 Size: 5866 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5669 AflIII A'CryG_T Cuts at: 1203 4765 5742 1203 Size: 3562 977 1327 Fragments arranged by size: 3562 1327 977 AgeI A'CCGG_T Cuts at: 4697 4697 Size: 5866 AhdI GACnn_n'nnGTC Cuts at: 4595 4595 Size: 5866 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5866 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2552 1184 Size: 1368 4498 AlwNI CAG_nnn'CTG Cuts at: 2347 2386 3609 5181 2347 Size: 39 1223 1572 3032 Fragments arranged by size: 3032 1572 1223 39 ApaLI G'TGCA_C Cuts at: 2475 2537 3923 5079 2475 Size: 62 1386 1156 3262 Fragments arranged by size: 3262 1386 1156 62 ApoI r'AATT_y Cuts at: 1493 3175 3401 3553 5662 1493 Size: 1682 226 152 2109 1697 Fragments arranged by size: 2109 1697 1682 226 152 AseI AT'TA_AT Cuts at: 160 1032 4420 160 Size: 872 3388 1606 Fragments arranged by size: 3388 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 AvaI C'yCGr_G Cuts at: 1870 1943 2561 3162 3191 1870 Size: 73 618 601 29 4545 Fragments arranged by size: 4545 618 601 73 29 AvrII C'CTAG_G Cuts at: 3097 3097 Size: 5866 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2478 2511 2478 Size: 33 5833 BamHI G'GATC_C Cuts at: 3196 3563 3196 Size: 367 5499 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2185 2841 3073 3149 3187 Size: 325 217 627 398 656 232 76 38 Cuts at: 3187 4642 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 656 627 398 325 232 217 76 38 BanII G_rGCy'C Cuts at: 1391 2220 2393 3185 1391 Size: 829 173 792 4072 Fragments arranged by size: 4072 829 792 173 BbeI G_GCGC'C Cuts at: 2189 2189 Size: 5866 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2415 928 Size: 971 516 4379 Fragments arranged by size: 4379 971 516 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4057 4091 1903 Size: 34 2120 34 3678 Fragments arranged by size: 3678 2120 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2506 3758 4968 2506 Size: 1252 1210 3404 Fragments arranged by size: 3404 1252 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5866 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2402 2970 4477 Size: 107 122 71 631 605 730 568 1507 Cuts at: 4477 136 Size: 1525 Fragments arranged by size: 1525 1507 730 631 605 568 122 107 71 BglII A'GATC_T Cuts at: 5861 5861 Size: 5866 BlpI GC'TnA_GC Cuts at: 3283 3283 Size: 5866 Bme1580I G_kGCm'C Cuts at: 1790 2479 2541 3078 3927 5083 1790 Size: 689 62 537 849 1156 2573 Fragments arranged by size: 2573 1156 849 689 537 62 BmgBI CAC'GTC Cuts at: 1318 2421 1318 Size: 1103 4763 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3597 4555 442 Size: 416 53 83 399 24 2180 958 1753 Fragments arranged by size: 2180 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5866 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2765 2909 3024 4526 1239 Size: 1526 144 115 1502 2579 Fragments arranged by size: 2579 1526 1502 144 115 Bpu10I CC'TnA_GC Cuts at: 2774 2774 Size: 5866 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3991 4856 5154 3991 Size: 865 298 4703 Fragments arranged by size: 4703 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2640 4529 882 Size: 617 1141 1889 2219 Fragments arranged by size: 2219 1889 1141 617 BsaAI yAC'GTr Cuts at: 493 1204 3657 493 Size: 711 2453 2702 Fragments arranged by size: 2702 2453 711 BsaBI GATnn'nnATC Cuts at: 5860 5860 Size: 5866 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2186 3673 4055 4690 Size: 53 83 186 936 653 1487 382 635 Cuts at: 4690 275 Size: 1451 Fragments arranged by size: 1487 1451 936 653 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3030 4299 4697 4971 5118 5529 3030 Size: 1269 398 274 147 411 3367 Fragments arranged by size: 3367 1269 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2137 2167 1178 Size: 30 165 30 734 30 4877 Fragments arranged by size: 4877 734 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3027 1186 Size: 1841 4025 BseYI C'CCAG_C Cuts at: 2250 2379 2386 2968 3054 5069 5565 2250 Size: 129 7 582 86 2015 496 2551 Fragments arranged by size: 2551 2015 582 496 129 86 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2801 1163 Size: 573 1065 4228 Fragments arranged by size: 4228 1065 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2298 2397 3316 4077 5105 1540 Size: 275 186 297 99 919 761 1028 2301 Fragments arranged by size: 2301 1028 919 761 297 275 186 99 BsiHKAI G_wGCw'C Cuts at: 2479 2541 3087 3927 4012 5083 2479 Size: 62 546 840 85 1071 3262 Fragments arranged by size: 3262 1071 840 546 85 62 BsmI GAATG_Cn' Cuts at: 3377 3470 3377 Size: 93 5773 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2220 2393 2479 2541 3078 3087 3185 Size: 399 430 173 86 62 537 9 98 Cuts at: 3185 3927 4012 5083 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 537 430 399 173 98 86 85 62 9 BspEI T'CCGG_A Cuts at: 5529 5529 Size: 5866 BspHI T'CATG_A Cuts at: 3755 5485 3755 Size: 1730 4136 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3221 844 Size: 2377 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2107 2761 3753 1394 Size: 429 284 654 992 3507 Fragments arranged by size: 3507 992 654 429 284 BsrDI GCAATG_nn' Cuts at: 65 1248 4361 4535 65 Size: 1183 3113 174 1396 Fragments arranged by size: 3113 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2293 4510 4697 1700 Size: 110 483 2217 187 2869 Fragments arranged by size: 2869 2217 483 187 110 BsrGI T'GTAC_A Cuts at: 96 3015 96 Size: 2919 2947 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5866 BssSI C'ACGA_G Cuts at: 2540 3926 4938 2540 Size: 1386 1012 3468 Fragments arranged by size: 3468 1386 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2317 2326 2347 794 Size: 1523 9 21 4313 Fragments arranged by size: 4313 1523 21 9 BstBI TT'CG_AA Cuts at: 4670 4670 Size: 5866 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5866 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5866 BstYI r'GATC_y Cuts at: 1972 3196 3563 3948 3965 5417 5503 5515 5861 Size: 1224 367 385 17 1452 86 12 346 Cuts at: 5861 1972 Size: 1977 Fragments arranged by size: 1977 1452 1224 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2124 2211 2298 2626 3091 513 Size: 552 1059 87 87 328 465 3288 Fragments arranged by size: 3288 1059 552 465 328 87 87 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2925 487 Size: 683 56 1699 3428 Fragments arranged by size: 3428 1699 683 56 BtsI GCAGTG_nn' Cuts at: 2541 3382 4176 4196 2541 Size: 841 794 20 4211 Fragments arranged by size: 4211 841 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5866 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5831 DraI TTT'AAA Cuts at: 3170 3170 Size: 5866 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2419 2828 4873 809 Size: 507 1103 409 2045 1802 Fragments arranged by size: 2045 1802 1103 507 409 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2295 2394 2898 Size: 54 1425 50 275 18 465 99 504 Cuts at: 2898 3313 4202 5831 8 Size: 415 889 1629 43 Fragments arranged by size: 1629 1425 889 504 465 415 275 99 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2295 2394 3313 1537 Size: 275 483 99 919 4090 Fragments arranged by size: 4090 919 483 275 99 EarI CTCTTCn'nnn_ Cuts at: 1353 3796 1353 Size: 2443 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2090 4453 4827 4973 387 Size: 1064 138 501 2363 374 146 1280 Fragments arranged by size: 2363 1280 1064 501 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2533 2765 2909 3024 3929 4526 5313 Size: 393 901 232 144 115 905 597 787 Cuts at: 5313 5581 1239 Size: 268 1524 Fragments arranged by size: 1524 905 901 787 597 393 268 232 144 115 EcoO109I rG'GnC_Cy Cuts at: 3101 3101 Size: 5866 EcoRI G'AATT_C Cuts at: 3175 3553 3175 Size: 378 5488 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2461 2733 2461 Size: 272 5594 FspI TGC'GCA Cuts at: 4372 4372 Size: 5866 HaeII r_GCGC'y Cuts at: 1678 2189 2810 2924 5013 1678 Size: 511 621 114 2089 2531 Fragments arranged by size: 2531 2089 621 511 114 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2239 2271 4507 4539 4581 Size: 32 135 32 667 32 2236 32 42 Cuts at: 4581 4613 1373 Size: 32 2626 Fragments arranged by size: 2626 2236 667 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3210 3456 669 Size: 988 281 1272 246 3079 Fragments arranged by size: 3079 1272 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5866 HpaI GTT'AAC Cuts at: 3456 3456 Size: 5866 KasI G'GCGC_C Cuts at: 2185 2185 Size: 5866 KpnI G_GTAC'C Cuts at: 2845 3191 2845 Size: 346 5520 MfeI C'AATT_G Cuts at: 3465 3465 Size: 5866 MluI A'CGCG_T Cuts at: 5742 5742 Size: 5866 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2748 4980 5164 1305 Size: 1443 2232 184 2007 Fragments arranged by size: 2232 2007 1443 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4044 Fragments arranged by size: 4044 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3824 4183 4342 518 Size: 648 843 1815 359 159 2042 Fragments arranged by size: 2042 1815 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2025 2126 2300 2347 2386 2879 3008 3058 Size: 419 101 174 47 39 493 129 50 Cuts at: 3058 3064 3282 3959 5107 5352 1606 Size: 6 218 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 493 419 245 218 174 129 101 50 47 39 6 NaeI GCC'GGC Cuts at: 2295 2295 Size: 5866 NarI GG'CG_CC Cuts at: 2186 2186 Size: 5866 NcoI C'CATG_G Cuts at: 513 1065 2211 513 Size: 552 1146 4168 Fragments arranged by size: 4168 1146 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5866 NgoMIV G'CCGG_C Cuts at: 2293 2293 Size: 5866 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5866 NotI GC'GGCC_GC Cuts at: 3313 3313 Size: 5866 NspI r_CATG'y Cuts at: 1132 1229 2475 3028 3224 4769 1132 Size: 97 1246 553 196 1545 2229 Fragments arranged by size: 2229 1545 1246 553 196 97 PacI TTA_AT'TAA Cuts at: 2436 2436 Size: 5866 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5866 PciI A'CATG_T Cuts at: 4765 4765 Size: 5866 PflMI CCAn_nnn'nTGG Cuts at: 1108 2386 1108 Size: 1278 4588 PmeI GTTT'AAAC Cuts at: 3170 3170 Size: 5866 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3935 3967 5448 5480 3935 Size: 32 1481 32 4321 Fragments arranged by size: 4321 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5866 PsiI TTA'TAA Cuts at: 3436 3436 Size: 5866 PspXI vC'TCGA_Gb Cuts at: 2561 2561 Size: 5866 PstI C_TGCA'G Cuts at: 830 2806 2928 3063 3218 830 Size: 1976 122 135 155 3478 Fragments arranged by size: 3478 1976 155 135 122 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5866 PvuII CAG'CTG Cuts at: 2386 3058 2386 Size: 672 5194 SacII CC_GC'GG Cuts at: 2127 2301 2127 Size: 174 5692 SalI G'TCGA_C Cuts at: 1936 3208 1936 Size: 1272 4594 SbfI CC_TGCA'GG Cuts at: 3218 3218 Size: 5866 ScaI AGT'ACT Cuts at: 4114 5826 4114 Size: 1712 4154 SexAI A'CCwGG_T Cuts at: 2837 2837 Size: 5866 SfcI C'TryA_G Cuts at: 826 1045 2482 2802 2924 3059 3214 4349 5030 Size: 219 1437 320 122 135 155 1135 681 Cuts at: 5030 5221 826 Size: 191 1471 Fragments arranged by size: 1471 1437 1135 681 320 219 191 155 135 122 SfiI GGCCn_nnn'nGGCC Cuts at: 2402 2402 Size: 5866 SfoI GGC'GCC Cuts at: 2187 2187 Size: 5866 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5866 SmaI CCC'GGG Cuts at: 1872 3164 3193 1872 Size: 1292 29 4545 Fragments arranged by size: 4545 1292 29 SmlI C'TyrA_G Cuts at: 820 1017 1943 2561 3970 4871 5133 820 Size: 197 926 618 1409 901 262 1553 Fragments arranged by size: 1553 1409 926 901 618 262 197 SnaBI TAC'GTA Cuts at: 493 3657 493 Size: 3164 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5866 SphI G_CATG'C Cuts at: 1132 3224 1132 Size: 2092 3774 SspI AAT'ATT Cuts at: 5 52 3790 5 Size: 47 3738 2081 Fragments arranged by size: 3738 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5866 StyI C'CwwG_G Cuts at: 513 1065 2211 3097 3305 513 Size: 552 1146 886 208 3074 Fragments arranged by size: 3074 1146 886 552 208 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2840 2973 3893 3910 4063 4248 4693 Size: 1255 1260 133 920 17 153 185 445 Cuts at: 4693 325 Size: 1498 Fragments arranged by size: 1498 1260 1255 920 445 185 153 133 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3015 4112 5824 Size: 274 80 33 51 1443 1038 1097 1712 Cuts at: 5824 96 Size: 138 Fragments arranged by size: 1712 1443 1097 1038 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2137 2169 5826 5858 2137 Size: 32 3657 32 2145 Fragments arranged by size: 3657 2145 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5866 XbaI T'CTAG_A Cuts at: 3202 3202 Size: 5866 XhoI C'TCGA_G Cuts at: 1943 2561 1943 Size: 618 5248 XmaI C'CCGG_G Cuts at: 1870 3162 3191 1870 Size: 1292 29 4545 Fragments arranged by size: 4545 1292 29 XmnI GAAnn'nnTTC Cuts at: 3995 3995 Size: 5866 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3674 276 Size: 53 83 186 936 2140 2468 Fragments arranged by size: 2468 2140 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PfoI PmlI PpuMI PspOMI PsrI RsrII SacI SanDI SapI SrfI SwaI XcmI