Restriction map for
pFN21AE5768
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII AccIII AclI AflIII AgeI
Alw44I Aor51HI ApaLI AscI Asp700I
AspI AviII AvrII BalI BamHI
BbrPI BcgI BciVI BcnI BglI
BglII BlnI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Cfr10I ClaI CpoI DraIII
DsaI EaeI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HapII
HgaI HincII HindII HpaI HpaII
KspI MluI MluNI MroI MscI
MspI NaeI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I XbaI XcmI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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Acc65I 150 GGTACC G^GTACC
AccI 2420 GTCTAC GT^[AC][GT]AC
AccII 1786 CGCG CG^CG
AcyI 2649 GGCGCC G[GA]^CG[CT]C
AhdI 1680 GACAGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 145 CCTCAGG CC^T[ACGT]AGG
ApaI 497 GGGCCC GGGCC^C
AseI 139 ATTAAT AT^TAAT
AsnI 139 ATTAAT AT^TAAT
Asp718I 150 GGTACC G^GTACC
AspEI 1680 GACAGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1297 GTGCTC G[AT]GC[AT]^C
AvaI 2016 CTCGAG C^[CT]CG[GA]G
BbeI 2649 GGCGCC GGCGC^C
BbsI 2368 GAAGAC GAAGAC(2/6)
BbvCI 2482 CCTCAGC CCTCAGC(-5/-2)
BclI 2376 TGATCA T^GATCA
BpuAI 2368 GAAGAC GAAGAC(2/6)
BsaAI 1584 TACGTG [CT]AC^GT[GA]
BsaBI 2736 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2649 GGCGCC G[GA]^CG[CT]C
BsgI 807 GTGCAG GTGCAG(16/14)
BsiHKAI 1297 GTGCTC G[AT]GC[AT]^C
BsmFI 877 GGGAC GGGAC(10/14)
BsmI 2169 GAATGC GAATGC(1/-1)
BsoBI 2016 CTCGAG C^[CT]CG[GA]G
Bsp1407I 912 TGTACA T^GTACA
BsrGI 912 TGTACA T^GTACA
BssSI 470 CACGAG CACGAG(-5/-1)
BstUI 1786 CGCG CG^CG
BstYI 1947 AGATCC [GA]^GATC[CT]
Bsu36I 145 CCTCAGG CC^T[ACGT]AGG
DrdI 925 GACAAAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1680 GACAGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 145 CCTCAGG CC^T[ACGT]AGG
EcoT14I 2176 CCAAGG C^C[AT][AT]GG
FbaI 2376 TGATCA T^GATCA
HaeII 2649 GGCGCC [GA]GCGC^[CT]
Hin1I 2649 GGCGCC G[GA]^CG[CT]C
KasI 2649 GGCGCC G^GCGCC
KpnI 150 GGTACC GGTAC^C
MamI 2736 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1947 AGATCC [GA]^GATC[CT]
MvnI 1786 CGCG CG^CG
NarI 2649 GGCGCC GG^CGCC
NdeI 314 CATATG CA^TATG
NspI 2067 GCATGT [GA]CATG^[CT]
PaeR7I 2016 CTCGAG C^TCGAG
PflMI 1936 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 139 ATTAAT AT^TAAT
PstI 1343 CTGCAG CTGCA^G
SexAI 2136 ACCAGGT A^CC[AT]GGT
SfoI 2649 GGCGCC GGC^GCC
SspBI 912 TGTACA T^GTACA
SspI 2158 AATATT AAT^ATT
StyI 2176 CCAAGG C^C[AT][AT]GG
Van91I 1936 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2016 CTCGAG C^TCGAG
XhoII 1947 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 1001 CCGC CCGC(-3/-1)
1259 GCGG
AflII 63 CTTAAG C^TTAAG
1186 CTTAAG
BanI 150 GGTACC G^G[CT][GA]CC
2649 GGCGCC
BanII 497 GGGCCC G[GA]GC[CT]^C
1082 GAGCCC
BfaI 1579 CTAG C^TAG
1685 CTAG
BfrI 63 CTTAAG C^TTAAG
1186 CTTAAG
BlpI 1299 GCTCAGC GC^T[ACGT]AGC
1708 GCTAAGC
Bpu1102I 1299 GCTCAGC GC^T[ACGT]AGC
1708 GCTAAGC
BstXI 956 CCAGCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1325 CCATTGCCCTGG
CelII 1299 GCTCAGC GC^T[ACGT]AGC
1708 GCTAAGC
CfoI 1787 GCGC GCG^C
2650 GCGC
DraI 664 TTTAAA TTT^AAA
1386 TTTAAA
DraII 21 AGGTCCT [GA]G^G[ACGT]CC[CT]
624 AGGTCCT
EarI 233 GAAGAG CTCTTC(1/4)
2332 GAAGAG
EcoO109I 21 AGGTCCT [GA]G^G[ACGT]CC[CT]
624 AGGTCCT
HhaI 1787 GCGC GCG^C
2650 GCGC
HinP1I 1787 GCGC G^CGC
2650 GCGC
Ksp632I 233 GAAGAG CTCTTC(1/4)
2332 GAAGAG
MaeI 1579 CTAG C^TAG
1685 CTAG
MfeI 1281 CAATTG C^AATTG
1525 CAATTG
MunI 1281 CAATTG C^AATTG
1525 CAATTG
PpuMI 21 AGGTCCT [GA]G^G[AT]CC[CT]
624 AGGTCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AvaII BbvI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HindIII HinfI HphI ItaI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I