Restriction map for
pFN21AE5768
(Linear) (Six-base) MAPSORT of: KIEE5768.seq Check: 6680 from: 1 to: 2829
KIEE5768.orf
With 182 enzymes: SgfI *
December 5, 2014 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1972 2829
Size: 1972 857
AccI GT'mk_AC
Cuts at: 0 2421 2829
Size: 2421 408
Acc65I G'GTAC_C
Cuts at: 0 150 2829
Size: 150 2679
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 218 2829
Size: 58 160 2611
Fragments arranged by size:
2611 160 58
AflII C'TTAA_G
Cuts at: 0 63 1186 2829
Size: 63 1123 1643
Fragments arranged by size:
1643 1123 63
AhdI GACnn_n'nnGTC
Cuts at: 0 1685 2829
Size: 1685 1144
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 65 97 2829
Size: 65 32 2732
Fragments arranged by size:
2732 65 32
AlwNI CAG_nnn'CTG
Cuts at: 0 844 973 1543 1816 1942 1987 2362 2829
Size: 844 129 570 273 126 45 375 467
Fragments arranged by size:
844 570 467 375 273 129 126 45
ApaI G_GGCC'C
Cuts at: 0 501 2829
Size: 501 2328
ApoI r'AATT_y
Cuts at: 0 751 1006 1031 1445 2009 2525 2582 2829
Size: 751 255 25 414 564 516 57 247
Fragments arranged by size:
751 564 516 414 255 247 57 25
AseI AT'TA_AT
Cuts at: 0 140 2829
Size: 140 2689
AvaI C'yCGr_G
Cuts at: 0 2016 2829
Size: 2016 813
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1133 1166 2829
Size: 1133 33 1663
Fragments arranged by size:
1663 1133 33
BanI G'GyrC_C
Cuts at: 0 150 2649 2829
Size: 150 2499 180
Fragments arranged by size:
2499 180 150
BanII G_rGCy'C
Cuts at: 0 501 1086 2829
Size: 501 585 1743
Fragments arranged by size:
1743 585 501
BbeI G_GCGC'C
Cuts at: 0 2653 2829
Size: 2653 176
BbsI GAAGACnn'nnnn_
Cuts at: 0 2375 2829
Size: 2375 454
BbvCI CC'TCA_GC
Cuts at: 0 2483 2829
Size: 2483 346
BclI T'GATC_A
Cuts at: 0 2376 2829
Size: 2376 453
BlpI GC'TnA_GC
Cuts at: 0 1300 1709 2829
Size: 1300 409 1120
Fragments arranged by size:
1300 1120 409
Bme1580I G_kGCm'C
Cuts at: 0 357 501 1721 1808 2829
Size: 357 144 1220 87 1021
Fragments arranged by size:
1220 1021 357 144 87
BmgBI CAC'GTC
Cuts at: 0 1372 2829
Size: 1372 1457
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1861 2829
Size: 1861 968
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 1378 1481 1964 2384 2829
Size: 113 1265 103 483 420 445
Fragments arranged by size:
1265 483 445 420 113 103
Bpu10I CC'TnA_GC
Cuts at: 0 2235 2483 2829
Size: 2235 248 346
Fragments arranged by size:
2235 346 248
BsaAI yAC'GTr
Cuts at: 0 1586 2829
Size: 1586 1243
BsaBI GATnn'nnATC
Cuts at: 0 2740 2829
Size: 2740 89
BsaHI Gr'CG_yC
Cuts at: 0 2650 2829
Size: 2650 179
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 80 110 1937 1967 2197 2227 2829
Size: 80 30 1827 30 230 30 602
Fragments arranged by size:
1827 602 230 80 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 884 2215 2218 2829
Size: 884 1331 3 611
Fragments arranged by size:
1331 884 611 3
BseYI C'CCAG_C
Cuts at: 0 1085 1809 2083 2097 2256 2829
Size: 1085 724 274 14 159 573
Fragments arranged by size:
1085 724 573 274 159 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 828 2829
Size: 828 2001
BsiHKAI G_wGCw'C
Cuts at: 0 1301 2829
Size: 1301 1528
BsmI GAATG_Cn'
Cuts at: 0 2175 2829
Size: 2175 654
Bsp1286I G_dGCh'C
Cuts at: 0 357 501 1086 1301 1721 1808 2829
Size: 357 144 585 215 420 87 1021
Fragments arranged by size:
1021 585 420 357 215 144 87
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 10 276 1972 2694 2829
Size: 10 266 1696 722 135
Fragments arranged by size:
1696 722 266 135 10
BsrDI GCAATG_nn'
Cuts at: 0 14 131 1049 1113 1325 2609 2639 2732
Size: 14 117 918 64 212 1284 30 93
Cuts at: 2732 2829
Size: 97
Fragments arranged by size:
1284 918 212 117 97 93 64 30
14
BsrGI T'GTAC_A
Cuts at: 0 912 2829
Size: 912 1917
BssSI C'ACGA_G
Cuts at: 0 470 2829
Size: 470 2359
BstXI CCAn_nnnn'nTGG
Cuts at: 0 963 1332 2829
Size: 963 369 1497
Fragments arranged by size:
1497 963 369
BstYI r'GATC_y
Cuts at: 0 1947 2829
Size: 1947 882
Bsu36I CC'TnA_GG
Cuts at: 0 146 2829
Size: 146 2683
BtsI GCAGTG_nn'
Cuts at: 0 1311 1919 2829
Size: 1311 608 910
Fragments arranged by size:
1311 910 608
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 490 525 2829
Size: 490 35 2304
Fragments arranged by size:
2304 490 35
DraI TTT'AAA
Cuts at: 0 666 1388 2829
Size: 666 722 1441
Fragments arranged by size:
1441 722 666
DrdI GACnn_nn'nnGTC
Cuts at: 0 931 2829
Size: 931 1898
EarI CTCTTCn'nnn_
Cuts at: 0 228 2327 2829
Size: 228 2099 502
Fragments arranged by size:
2099 502 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 113 218 1378 1481 1964 2384 2829
Size: 58 55 105 1160 103 483 420 445
Fragments arranged by size:
1160 483 445 420 105 103 58 55
EcoO109I rG'GnC_Cy
Cuts at: 0 22 625 2829
Size: 22 603 2204
Fragments arranged by size:
2204 603 22
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 755 2235 2829
Size: 755 1480 594
Fragments arranged by size:
1480 755 594
HaeII r_GCGC'y
Cuts at: 0 2653 2829
Size: 2653 176
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 470 502 1351 1383 1671 1703 1932 1964
Size: 470 32 849 32 288 32 229 32
Cuts at: 1964 2157 2189 2403 2435 2599 2631 2829
Size: 193 32 214 32 164 32 198
Fragments arranged by size:
849 470 288 229 214 198 193 164
32 32 32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 200 658 1226 1270 2829
Size: 200 458 568 44 1559
Fragments arranged by size:
1559 568 458 200 44
KasI G'GCGC_C
Cuts at: 0 2649 2829
Size: 2649 180
KpnI G_GTAC'C
Cuts at: 0 154 2829
Size: 154 2675
MfeI C'AATT_G
Cuts at: 0 1281 1525 2829
Size: 1281 244 1304
Fragments arranged by size:
1304 1281 244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1817 2829
Size: 1817 1012
MslI CAynn'nnrTG
Cuts at: 0 362 610 2282 2829
Size: 362 248 1672 547
Fragments arranged by size:
1672 547 362 248
MspA1I CmG'CkG
Cuts at: 0 457 1259 1801 2269 2829
Size: 457 802 542 468 560
Fragments arranged by size:
802 560 542 468 457
NarI GG'CG_CC
Cuts at: 0 2650 2829
Size: 2650 179
NdeI CA'TA_TG
Cuts at: 0 315 2829
Size: 315 2514
NspI r_CATG'y
Cuts at: 0 2071 2829
Size: 2071 758
PasI CC'CwG_GG
Cuts at: 0 1332 2829
Size: 1332 1497
PflMI CCAn_nnn'nTGG
Cuts at: 0 1942 2829
Size: 1942 887
PfoI T'CCnGG_A
Cuts at: 0 1698 2829
Size: 1698 1131
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 157 189 2829
Size: 157 32 2640
Fragments arranged by size:
2640 157 32
PpuMI rG'GwC_Cy
Cuts at: 0 22 625 2829
Size: 22 603 2204
Fragments arranged by size:
2204 603 22
PsiI TTA'TAA
Cuts at: 0 1996 2829
Size: 1996 833
PspOMI G'GGCC_C
Cuts at: 0 497 2829
Size: 497 2332
PspXI vC'TCGA_Gb
Cuts at: 0 2016 2829
Size: 2016 813
PstI C_TGCA'G
Cuts at: 0 1347 2829
Size: 1347 1482
PvuII CAG'CTG
Cuts at: 0 457 1801 2269 2829
Size: 457 1344 468 560
Fragments arranged by size:
1344 560 468 457
SexAI A'CCwGG_T
Cuts at: 0 2136 2829
Size: 2136 693
SfcI C'TryA_G
Cuts at: 0 793 1343 2422 2829
Size: 793 550 1079 407
Fragments arranged by size:
1079 793 550 407
SfoI GGC'GCC
Cuts at: 0 2651 2829
Size: 2651 178
SmlI C'TyrA_G
Cuts at: 0 63 1186 2016 2829
Size: 63 1123 830 813
Fragments arranged by size:
1123 830 813 63
SspI AAT'ATT
Cuts at: 0 2160 2829
Size: 2160 669
StyI C'CwwG_G
Cuts at: 0 2176 2829
Size: 2176 653
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 712 2829
Size: 712 2117
TatI w'GTAC_w
Cuts at: 0 96 912 1495 2829
Size: 96 816 583 1334
Fragments arranged by size:
1334 816 583 96
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 416 448 1187 1219 2197 2229 2829
Size: 416 32 739 32 978 32 600
Fragments arranged by size:
978 739 600 416 32 32 32
XhoI C'TCGA_G
Cuts at: 0 2016 2829
Size: 2016 813
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AhdI AloI AlwNI
ApaI ApoI AseI AvaI BaeI BanI BanII BbeI
BbsI BbvCI BclI BlpI Bme1580I BmgBI BplI BpmI
Bpu10I BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrGI BssSI BstXI
BstYI Bsu36I BtsI CspCI DraI DrdI EarI Eco57MI
EcoO109I FalI HaeII Hin4I HindIII KasI KpnI MfeI
MmeI MslI MspA1I NarI NdeI NspI PasI PflMI
PfoI PpiI PpuMI PsiI PspOMI PspXI PstI PvuII
SexAI SfcI SfoI SmlI SspI StyI TaqII TatI
TstI XhoI
Enzymes that do not cut:
AatII AclI AfeI AflIII AgeI AleI AlfI ApaLI
AscI AsiSI AvrII BamHI BcgI BciVI BfrBI BglI
BglII BmrI BmtI BpuEI BsaI BsaWI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrFI BssHII BstAPI BstBI
BstEII BstZ17I BtgI BtgZI ClaI DraIII EaeI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HincII HpaI MluI MscI NaeI NcoI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PmlI PshAI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI Tth111I XbaI XcmI XmaI
XmnI ZraI