################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## Acc65I AclI AfaI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BclI BglI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaI BsiHKAI BsiWI BsmFI BsmI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstNI BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MamI MflI MluI MluNI MscI MslI MspA1I MvaI NcoI NheI NotI NruI NsiI NspV PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TthHB8I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AatII 115 GACGTC GACGT^C AccI 847 GTAGAC GT^[AC][GT]AC AccIII 213 TCCGGA T^CCGGA AcsI 409 GAATTT [GA]^AATT[CT] AflII 592 CTTAAG C^TTAAG AlwI 771 GATCC GGATC(4/5) ApoI 409 GAATTT [GA]^AATT[CT] AspI 217 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 759 GGACC G^G[AT]CC BanI 282 GGCGCC G^G[CT][GA]CC BanII 7 GGGCTC G[GA]GC[CT]^C BbeI 282 GGCGCC GGCGC^C BbsI 223 GTCTTC GAAGAC(2/6) BciVI 723 GTATCC GTATCC(6/5) BfaI 569 CTAG C^TAG BfrI 592 CTTAAG C^TTAAG BpuAI 223 GTCTTC GAAGAC(2/6) BsaAI 148 TACGTG [CT]AC^GT[GA] BsaWI 213 TCCGGA [AT]^CCGG[AT] BseAI 213 TCCGGA T^CCGGA BsmBI 256 GAGACG CGTCTC(1/5) BspEI 213 TCCGGA T^CCGGA BsrFI 195 GCCGGC [GA]^CCGG[CT] BstAPI 693 GCACGTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 804 GGATG GGATG(2/0) Cfr10I 195 GCCGGC [GA]^CCGG[CT] DraII 587 AGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 43 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 478 GACTATGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 587 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoT14I 98 CCAAGG C^C[AT][AT]GG FokI 804 GGATG GGATG(9/13) HgaI 221 GCGTC GACGC(5/10) HincII 304 GTTGAC GT[CT]^[GA]AC HindII 304 GTTGAC GT[CT]^[GA]AC KasI 282 GGCGCC G^GCGCC MaeI 569 CTAG C^TAG MaeIII 614 GTGAC ^GT[ACGT]AC MfeI 560 CAATTG C^AATTG MroI 213 TCCGGA T^CCGGA MunI 560 CAATTG C^AATTG NaeI 195 GCCGGC GCC^GGC NarI 282 GGCGCC GG^CGCC NdeI 763 CATATG CA^TATG NgoMIV 195 GCCGGC G^CCGGC NlaIV 282 GGCGCC GG[ACGT]^[ACGT]CC NspI 782 GCATGT [GA]CATG^[CT] PacI 832 TTAATTAA TTAAT^TAA PflFI 217 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 42 CCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SfoI 282 GGCGCC GGC^GCC SmaI 277 CCCGGG CCC^GGG SmlI 592 CTTAAG C^T[CT][GA]AG StyI 98 CCAAGG C^C[AT][AT]GG TfiI 574 GAATC G^A[AT]TC Tsp45I 614 GTGAC ^GT[GC]AC Tth111I 217 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 42 CCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 277 CCCGGG C^CCGGG XmaI 277 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 115 GACGTC G[GA]^CG[CT]C 282 GGCGCC Asp700I 328 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 522 GAAGAGTTTC AvaI 64 CCCGAG C^[CT]CG[GA]G 277 CCCGGG BmyI 7 GGGCTC G[AGT]GC[ACT]^C 691 GGGCAC BsaHI 115 GACGTC G[GA]^CG[CT]C 282 GGCGCC BseRI 106 GAGGAG GAGGAG(10/8) 253 GAGGAG BsgI 578 CTGCAC GTGCAG(16/14) 703 CTGCAC BsiEI 30 CGGCCG CG[GA][CT]^CG 197 CGGCCG BsmAI 256 GAGAC GTCTC(1/5) 487 GTCTC BsoBI 64 CCCGAG C^[CT]CG[GA]G 277 CCCGGG Bsp1286I 7 GGGCTC G[AGT]GC[ACT]^C 691 GGGCAC BspMI 817 ACCTGC ACCTGC(4/8) 180 GCAGGT BsrI 792 ACTGG ACTGG(1/-1) 499 CCAGT DdeI 88 CTGAG C^T[ACGT]AG 825 CTCAG DsaI 47 CCGTGG C^C[GA][CT]GG 242 CCGTGG EagI 30 CGGCCG C^GGCCG 197 CGGCCG EarI 522 GAAGAG CTCTTC(1/4) 718 GAAGAG EclXI 30 CGGCCG C^GGCCG 197 CGGCCG Eco52I 30 CGGCCG C^GGCCG 197 CGGCCG Hin1I 115 GACGTC G[GA]^CG[CT]C 282 GGCGCC HphI 269 TCACC GGTGA(8/7) 815 TCACC Ksp632I 522 GAAGAG CTCTTC(1/4) 718 GAAGAG PleI 850 GACTC GAGTC(4/5) 67 GAGTC PstI 178 CTGCAG CTGCA^G 531 CTGCAG SfcI 178 CTGCAG C^T[GA][CT]AG 531 CTGCAG XcmI 38 CCACCCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 491 CCACAGTGCCAGTGG XmnI 328 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 522 GAAGAGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AccII AciI AluI BbvI BcnI BsaJI BsiYI BslI BssKI BstUI Cac8I CfoI Cfr13I DpnII EaeI FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeII MboI MboII MnlI MseI MspI MvnI MwoI NciI NdeII NlaIII Sau3AI Sau96I ScrFI Tru9I TseI Tsp509I TspRI