################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AviII AvrII BalI BamHI BanII BbeI BbrPI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HinfI HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 156 CGCG CG^CG AcsI 178 AAATTT [GA]^AATT[CT] AcyI 953 GGCGTC G[GA]^CG[CT]C AlwI 623 GGATC GGATC(4/5) ApoI 178 AAATTT [GA]^AATT[CT] AspI 573 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 759 CCCGGG C^[CT]CG[GA]G BanI 904 GGCACC G^G[CT][GA]CC BbsI 714 GAAGAC GAAGAC(2/6) BbvCI 981 CCTCAGC CCTCAGC(-5/-2) BclI 164 TGATCA T^GATCA BpuAI 714 GAAGAC GAAGAC(2/6) BsaAI 628 TACGTG [CT]AC^GT[GA] BsaHI 953 GGCGTC G[GA]^CG[CT]C BseRI 778 CTCCTC GAGGAG(10/8) BsmAI 660 GTCTC GTCTC(1/5) BsoBI 759 CCCGGG C^[CT]CG[GA]G BspHI 392 TCATGA T^CATGA BspMI 617 ACCTGC ACCTGC(4/8) BstUI 156 CGCG CG^CG BstYI 623 GGATCT [GA]^GATC[CT] CfoI 844 GCGC GCG^C DsaI 371 CCGTGG C^C[GA][CT]GG EarI 528 CTCTTC CTCTTC(1/4) EcoT14I 1029 CCAAGG C^C[AT][AT]GG FauI 402 GCGGG CCCGC(4/6) FbaI 164 TGATCA T^GATCA HaeII 843 GGCGCT [GA]GCGC^[CT] HgaI 954 GCGTC GACGC(5/10) HhaI 844 GCGC GCG^C Hin1I 953 GGCGTC G[GA]^CG[CT]C HinP1I 844 GCGC G^CGC HincII 295 GTCAAC GT[CT]^[GA]AC HindII 295 GTCAAC GT[CT]^[GA]AC HindIII 85 AAGCTT A^AGCTT Ksp632I 528 CTCTTC CTCTTC(1/4) MflI 623 GGATCT [GA]^GATC[CT] MseI 263 TTAA T^TAA MspA1I 300 CCGCTG C[AC]G^C[GT]G MvnI 156 CGCG CG^CG PflFI 573 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 795 CTGCAG CTGCA^G RcaI 392 TCATGA T^CATGA SapI 527 GCTCTTC GCTCTTC(1/4) SfcI 795 CTGCAG C^T[GA][CT]AG SmaI 759 CCCGGG CCC^GGG StyI 1029 CCAAGG C^C[AT][AT]GG Tru9I 263 TTAA T^TAA Tth111I 573 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 623 GGATCT [GA]^GATC[CT] XmaCI 759 CCCGGG C^CCGGG XmaI 759 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BcnI 759 CCCGG CC^[GC]GG 760 CCGGG BsgI 811 GTGCAG GTGCAG(16/14) 1034 GTGCAG BspLU11I 383 ACATGT A^CATGT 896 ACATGT BstF5I 966 CATCC GGATG(2/0) 398 GGATG BstNI 534 CCTGG CC^[AT]GG 765 CCTGG EcoRII 534 CCTGG ^CC[AT]GG 765 CCTGG FokI 966 CATCC GGATG(9/13) 398 GGATG HapII 690 CCGG C^CGG 760 CCGG HpaII 690 CCGG C^CGG 760 CCGG MspI 690 CCGG C^CGG 760 CCGG MvaI 534 CCTGG CC^[AT]GG 765 CCTGG NciI 759 CCCGG CC^[GC]GG 760 CCGGG SfaNI 399 GATGC GCATC(5/9) 965 GCATC TaqI 65 TCGA T^CGA 782 TCGA TthHB8I 65 TCGA T^CGA 782 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AciI AfaI AflIII AluI AvaII BbvI BsaJI BsiYI BslI BsmFI BsrI BssKI BstXI Cac8I Cfr13I DdeI DpnII Fnu4HI HaeIII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MwoI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI TseI Tsp45I Tsp509I TspRI