################################## # NO CUT ENZYMES ( 195 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AgeI AhdI AlwI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaII KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 229 GTCTAC GT^[AC][GT]AC AfaI 395 GTAC GT^AC AflIII 334 ACATGT A^C[GA][CT]GT Alw44I 12 GTGCAC G^TGCAC ApaLI 12 GTGCAC G^TGCAC AspHI 12 GTGCAC G[AT]GC[AT]^C BanI 316 GGCACC G^G[CT][GA]CC BbsI 153 GAAGAC GAAGAC(2/6) BcgI 120 CGACATCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 133 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 153 GAAGAC GAAGAC(2/6) BsaI 344 GAGACC GGTCTC(1/5) BseRI 49 CTCCTC GAGGAG(10/8) BsiHKAI 12 GTGCAC G[AT]GC[AT]^C BsiYI 398 CCCAGAATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 398 CCCAGAATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 344 GAGAC GTCTC(1/5) BsmFI 75 GGGAC GGGAC(10/14) BspLU11I 334 ACATGT A^CATGT BstF5I 137 GGATG GGATG(2/0) DpnII 286 GATC ^GATC FokI 137 GGATG GGATG(9/13) HincII 165 GTTAAC GT[CT]^[GA]AC HindII 165 GTTAAC GT[CT]^[GA]AC HpaI 165 GTTAAC GTT^AAC HphI 53 TCACC GGTGA(8/7) MboI 286 GATC ^GATC MboII 153 GAAGA GAAGA(8/7) MspA1I 7 CAGCTG C[AC]G^C[GT]G MwoI 133 GCCTGGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 286 GATC ^GATC NspI 334 ACATGT [GA]CATG^[CT] PleI 357 GAGTC GAGTC(4/5) PvuII 7 CAGCTG CAG^CTG RsaI 395 GTAC GT^AC Sau3AI 286 GATC ^GATC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AcsI 82 AAATTC [GA]^AATT[CT] 410 AAATTT ApoI 82 AAATTC [GA]^AATT[CT] 410 AAATTT BbvI 130 GCAGC GCAGC(8/12) 180 GCAGC BmyI 12 GTGCAC G[AGT]GC[ACT]^C 315 GGGCAC Bsp1286I 12 GTGCAC G[AGT]GC[ACT]^C 315 GGGCAC Cac8I 22 GCCAGC GC[ACGT]^[ACGT]GC 44 GCAGGC Cfr13I 20 GGGCC G^G[ACGT]CC 69 GGCCC Fnu4HI 130 GCAGC GC^[ACGT]GC 180 GCAGC HaeIII 21 GGCC GG^CC 69 GGCC ItaI 130 GCAGC GC^[ACGT]GC 180 GCAGC MseI 166 TTAA T^TAA 248 TTAA NlaIV 47 GGCTCC GG[ACGT]^[ACGT]CC 316 GGCACC Sau96I 20 GGGCC G^G[ACGT]CC 69 GGCCC Tru9I 166 TTAA T^TAA 248 TTAA TseI 130 GCAGC G^C[AT]GC 180 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### AluI BsaJI BssKI BstNI EcoRII HinfI MnlI MvaI NlaIII ScrFI TfiI Tsp509I