################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstNI BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRII EcoT14I EcoT22I FbaI FseI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MluI MroI MspA1I MvaI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AfaI 146 GTAC GT^AC AluI 50 AGCT AG^CT AlwNI 1270 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 208 ATTAAT AT^TAAT AsnI 208 ATTAAT AT^TAAT AspHI 526 GAGCAC G[AT]GC[AT]^C AviII 44 TGCGCA TGC^GCA BbsI 74 GTCTTC GAAGAC(2/6) BcnI 1146 CCGGG CC^[GC]GG BglII 1126 AGATCT A^GATCT BmyI 526 GAGCAC G[AGT]GC[ACT]^C BpuAI 74 GTCTTC GAAGAC(2/6) BsaBI 31 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 1259 CTGCAC GTGCAG(16/14) BsiHKAI 526 GAGCAC G[AT]GC[AT]^C BsmAI 557 GAGAC GTCTC(1/5) Bsp1286I 526 GAGCAC G[AGT]GC[ACT]^C BspHI 1151 TCATGA T^CATGA BsrBI 718 GAGCGG CCGCTC(-3/-3) BssKI 1146 CCGGG ^CC[ACGT]GG BstAPI 1269 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 669 CCATGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1126 AGATCT [GA]^GATC[CT] CfoI 45 GCGC GCG^C DpnII 1127 GATC ^GATC DraI 1113 TTTAAA TTT^AAA DraII 565 AGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 949 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO109I 565 AGGCCCT [GA]G^G[ACGT]CC[CT] FauI 1278 GCGGG CCCGC(4/6) FspI 44 TGCGCA TGC^GCA HapII 1146 CCGG C^CGG HhaI 45 GCGC GCG^C HinP1I 45 GCGC G^CGC HpaII 1146 CCGG C^CGG MamI 31 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 1127 GATC ^GATC MfeI 1328 CAATTG C^AATTG MflI 1126 AGATCT [GA]^GATC[CT] MslI 664 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 1146 CCGG C^CGG MunI 1328 CAATTG C^AATTG NciI 1146 CCGGG CC^[GC]GG NdeI 1055 CATATG CA^TATG NdeII 1127 GATC ^GATC PleI 72 GAGTC GAGTC(4/5) PshBI 208 ATTAAT AT^TAAT PstI 1283 CTGCAG CTGCA^G RcaI 1151 TCATGA T^CATGA RsaI 146 GTAC GT^AC Sau3AI 1127 GATC ^GATC ScrFI 1146 CCGGG CC^[ACGT]GG SmlI 1312 CTTGAG C^T[CT][GA]AG SspI 1189 AATATT AAT^ATT XbaI 393 TCTAGA T^CTAGA XcmI 1187 CCAATATTTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1126 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AciI 720 GCGG CCGC(-3/-1) 1278 GCGG AcsI 386 AAATTT [GA]^AATT[CT] 590 AAATTC ApoI 386 AAATTT [GA]^AATT[CT] 590 AAATTC Asp700I 59 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 460 GAAGTTTTTC AvaII 610 GGTCC G^G[AT]CC 1230 GGACC BsiYI 686 CCGTAGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1233 CCCCATGAAGG BslI 686 CCGTAGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1233 CCCCATGAAGG BsmI 906 GCATTC GAATGC(1/-1) 1175 GCATTC Cac8I 47 GCAAGC GC[ACGT]^[ACGT]GC 1278 GCGGGC EcoRV 700 GATATC GAT^ATC 869 GATATC HphI 704 TCACC GGTGA(8/7) 920 TCACC NlaIV 609 GGGTCC GG[ACGT]^[ACGT]CC 1230 GGACCC SfaNI 128 GATGC GCATC(5/9) 1272 GATGC TfiI 241 GATTC G^A[AT]TC 945 GATTC XmnI 59 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 460 GAAGTTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### BalI BbvI BfaI BsrI BstF5I Cfr13I DdeI EaeI Fnu4HI FokI HaeIII HinfI ItaI MaeI MaeIII MboII MluNI MnlI MscI MseI MwoI NlaIII Sau96I SfcI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I