(Circular) (Six-base) MAPSORT of: pFN21AE3881.seq Check: 661 from: 1 to: 6061 pFN21AE3881 6061 bp With 182 enzymes: SgfI * August 4, 2021 21:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3871 278 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3404 1937 Size: 1467 4594 Acc65I G'GTAC_C Cuts at: 3382 3382 Size: 6061 AclI AA'CG_TT Cuts at: 1563 4188 4561 1563 Size: 2625 373 3063 Fragments arranged by size: 3063 2625 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2960 3204 4124 5508 5776 1632 Size: 1328 244 920 1384 268 1917 Fragments arranged by size: 1917 1384 1328 920 268 244 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5864 AflIII A'CryG_T Cuts at: 1203 4960 5937 1203 Size: 3757 977 1327 Fragments arranged by size: 3757 1327 977 AgeI A'CCGG_T Cuts at: 4892 4892 Size: 6061 AhdI GACnn_n'nnGTC Cuts at: 4790 4790 Size: 6061 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6061 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2842 1184 Size: 1658 4403 AlwNI CAG_nnn'CTG Cuts at: 3280 3804 5376 3280 Size: 524 1572 3965 Fragments arranged by size: 3965 1572 524 ApaLI G'TGCA_C Cuts at: 4118 5274 4118 Size: 1156 4905 ApoI r'AATT_y Cuts at: 1493 2391 2595 3370 3596 3748 5857 1493 Size: 898 204 775 226 152 2109 1697 Fragments arranged by size: 2109 1697 898 775 226 204 152 AseI AT'TA_AT Cuts at: 160 1032 2214 4615 160 Size: 872 1182 2401 1606 Fragments arranged by size: 2401 1606 1182 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 AvaI C'yCGr_G Cuts at: 1870 1943 3386 1870 Size: 73 1443 4545 Fragments arranged by size: 4545 1443 73 BamHI G'GATC_C Cuts at: 3391 3758 3391 Size: 367 5694 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3382 4837 618 Size: 325 217 627 1595 1455 1842 Fragments arranged by size: 1842 1595 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3380 1391 Size: 1989 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2072 928 Size: 971 173 4917 Fragments arranged by size: 4917 971 173 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4252 4286 1903 Size: 34 2315 34 3678 Fragments arranged by size: 3678 2315 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3953 5163 3953 Size: 1210 4851 BclI T'GATC_A Cuts at: 1548 1548 Size: 6061 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4672 136 Size: 107 122 71 631 605 3000 1525 Fragments arranged by size: 3000 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3131 6056 3131 Size: 2925 3136 BlpI GC'TnA_GC Cuts at: 3478 3478 Size: 6061 Bme1580I G_kGCm'C Cuts at: 1790 4122 5278 1790 Size: 2332 1156 2573 Fragments arranged by size: 2573 2332 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6061 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3792 4750 442 Size: 416 53 83 399 24 2375 958 1753 Fragments arranged by size: 2375 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6061 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4721 1239 Size: 3482 2579 Bpu10I CC'TnA_GC Cuts at: 2907 2907 Size: 6061 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3338 4186 5051 5349 3338 Size: 848 865 298 4050 Fragments arranged by size: 4050 865 848 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4724 882 Size: 617 3225 2219 Fragments arranged by size: 3225 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3852 493 Size: 711 2648 2702 Fragments arranged by size: 2702 2648 711 BsaBI GATnn'nnATC Cuts at: 2040 6055 2040 Size: 4015 2046 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3868 4250 4885 275 Size: 53 83 186 936 2335 382 635 1451 Fragments arranged by size: 2335 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4494 4892 5166 5313 5724 4494 Size: 398 274 147 411 4831 Fragments arranged by size: 4831 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5836 Fragments arranged by size: 5836 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6061 BseYI C'CCAG_C Cuts at: 5264 5760 5264 Size: 496 5565 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3249 1163 Size: 573 1513 3975 Fragments arranged by size: 3975 1513 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3511 4272 5300 1540 Size: 275 186 1510 761 1028 2301 Fragments arranged by size: 2301 1510 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2535 4122 4207 5278 2535 Size: 1587 85 1071 3318 Fragments arranged by size: 3318 1587 1071 85 BsmI GAATG_Cn' Cuts at: 2911 3180 3572 3665 2911 Size: 269 392 93 5307 Fragments arranged by size: 5307 392 269 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2535 3380 4122 4207 5278 1391 Size: 399 745 845 742 85 1071 2174 Fragments arranged by size: 2174 1071 845 745 742 399 85 BspEI T'CCGG_A Cuts at: 5724 5724 Size: 6061 BspHI T'CATG_A Cuts at: 3156 3950 5680 3156 Size: 794 1730 3537 Fragments arranged by size: 3537 1730 794 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3416 844 Size: 2572 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2725 3948 1394 Size: 429 902 1223 3507 Fragments arranged by size: 3507 1223 902 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4556 4730 65 Size: 1183 3308 174 1396 Fragments arranged by size: 3308 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4705 4892 1700 Size: 110 2895 187 2869 Fragments arranged by size: 2895 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6061 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6061 BssSI C'ACGA_G Cuts at: 4121 5133 4121 Size: 1012 5049 BstAPI GCAn_nnn'nTGC Cuts at: 794 3280 794 Size: 2486 3575 BstBI TT'CG_AA Cuts at: 4865 4865 Size: 6061 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6061 BstXI CCAn_nnnn'nTGG Cuts at: 2681 2681 Size: 6061 BstYI r'GATC_y Cuts at: 1972 3131 3391 3758 4143 4160 5612 5698 5710 Size: 1159 260 367 385 17 1452 86 12 Cuts at: 5710 6056 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1159 385 367 346 260 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4570 Fragments arranged by size: 4570 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5322 Fragments arranged by size: 5322 683 56 BtsI GCAGTG_nn' Cuts at: 3100 3577 4371 4391 3100 Size: 477 794 20 4770 Fragments arranged by size: 4770 794 477 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6061 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6026 DraI TTT'AAA Cuts at: 3120 3365 3120 Size: 245 5816 DraIII CAC_nnn'GTG Cuts at: 2959 2959 Size: 6061 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5068 809 Size: 507 3752 1802 Fragments arranged by size: 3752 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2440 2671 2683 Size: 54 1425 50 275 18 610 231 12 Cuts at: 2683 3508 4397 6026 8 Size: 825 889 1629 43 Fragments arranged by size: 1629 1425 889 825 610 275 231 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 3508 1537 Size: 275 1696 4090 Fragments arranged by size: 4090 1696 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3991 1353 Size: 2638 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4648 5022 5168 387 Size: 1064 138 3059 374 146 1280 Fragments arranged by size: 3059 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2960 3204 4124 4721 5508 5776 1239 Size: 393 1328 244 920 597 787 268 1524 Fragments arranged by size: 1524 1328 920 787 597 393 268 244 EcoO109I rG'GnC_Cy Cuts at: 2571 2571 Size: 6061 EcoRI G'AATT_C Cuts at: 3370 3748 3370 Size: 378 5683 EcoRV GAT'ATC Cuts at: 2707 2876 2707 Size: 169 5892 FspI TGC'GCA Cuts at: 2051 4567 2051 Size: 2516 3545 HaeII r_GCGC'y Cuts at: 1678 5208 1678 Size: 3530 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2966 2998 4702 4734 4776 Size: 32 135 32 1394 32 1704 32 42 Cuts at: 4776 4808 1373 Size: 32 2626 Fragments arranged by size: 2626 1704 1394 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3405 3651 669 Size: 988 281 1467 246 3079 Fragments arranged by size: 3079 1467 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6061 HpaI GTT'AAC Cuts at: 3651 3651 Size: 6061 KpnI G_GTAC'C Cuts at: 3386 3386 Size: 6061 MfeI C'AATT_G Cuts at: 3333 3660 3333 Size: 327 5734 MluI A'CGCG_T Cuts at: 5937 5937 Size: 6061 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3187 5175 5359 1305 Size: 1882 1988 184 2007 Fragments arranged by size: 2007 1988 1882 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2442 2673 2685 10 Size: 54 1425 343 610 231 12 3386 Fragments arranged by size: 3386 1425 610 343 231 54 12 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2673 4019 4378 4537 518 Size: 648 843 664 1346 359 159 2042 Fragments arranged by size: 2042 1346 843 664 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3477 4154 5302 5547 1606 Size: 1871 677 1148 245 2120 Fragments arranged by size: 2120 1871 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4570 Fragments arranged by size: 4570 939 552 NdeI CA'TA_TG Cuts at: 387 3061 387 Size: 2674 3387 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6061 NotI GC'GGCC_GC Cuts at: 3508 3508 Size: 6061 NspI r_CATG'y Cuts at: 1132 1229 3419 4964 1132 Size: 97 2190 1545 2229 Fragments arranged by size: 2229 2190 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6061 PciI A'CATG_T Cuts at: 4960 4960 Size: 6061 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6061 PmeI GTTT'AAAC Cuts at: 3365 3365 Size: 6061 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4130 4162 5643 5675 4130 Size: 32 1481 32 4516 Fragments arranged by size: 4516 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6061 PsiI TTA'TAA Cuts at: 3631 3631 Size: 6061 PstI C_TGCA'G Cuts at: 830 3292 3413 830 Size: 2462 121 3478 Fragments arranged by size: 3478 2462 121 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6061 SalI G'TCGA_C Cuts at: 1936 3403 1936 Size: 1467 4594 SbfI CC_TGCA'GG Cuts at: 3413 3413 Size: 6061 ScaI AGT'ACT Cuts at: 4309 6021 4309 Size: 1712 4349 SfcI C'TryA_G Cuts at: 826 1045 2232 2566 3288 3409 4544 5225 5416 Size: 219 1187 334 722 121 1135 681 191 Cuts at: 5416 826 Size: 1471 Fragments arranged by size: 1471 1187 1135 722 681 334 219 191 121 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6061 SmaI CCC'GGG Cuts at: 1872 3388 1872 Size: 1516 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3317 4165 5066 5328 820 Size: 197 926 1374 848 901 262 1553 Fragments arranged by size: 1553 1374 926 901 848 262 197 SnaBI TAC'GTA Cuts at: 493 3852 493 Size: 3359 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6061 SphI G_CATG'C Cuts at: 1132 3419 1132 Size: 2287 3774 SspI AAT'ATT Cuts at: 5 52 3196 3985 5 Size: 47 3144 789 2081 Fragments arranged by size: 3144 2081 789 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6061 StyI C'CwwG_G Cuts at: 513 1065 2004 3500 513 Size: 552 939 1496 3074 Fragments arranged by size: 3074 1496 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4088 4105 4258 4443 4888 325 Size: 1255 2508 17 153 185 445 1498 Fragments arranged by size: 2508 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2150 4307 6019 Size: 274 80 33 51 1443 173 2157 1712 Cuts at: 6019 96 Size: 138 Fragments arranged by size: 2157 1712 1443 274 173 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2941 2973 6021 6053 2941 Size: 32 3048 32 2949 Fragments arranged by size: 3048 2949 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6061 XbaI T'CTAG_A Cuts at: 2398 3397 2398 Size: 999 5062 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3199 3199 Size: 6061 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6061 XmaI C'CCGG_G Cuts at: 1870 3386 1870 Size: 1516 4545 XmnI GAAnn'nnTTC Cuts at: 2068 2469 4190 2068 Size: 401 1721 3939 Fragments arranged by size: 3939 1721 401 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3869 276 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI