################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BanI BanII BbeI BbrPI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MluI MroI MslI MspA1I MwoI NaeI NarI NcoI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 843 GTCTAC GT^[AC][GT]AC AccII 549 CGCG CG^CG AflIII 180 ACATGT A^C[GA][CT]GT Asp700I 1039 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 120 TGGCCA TGG^CCA BbvCI 18 CCTCAGC CCTCAGC(-5/-2) BcnI 24 CCGGG CC^[GC]GG BmyI 1124 GGGCAC G[AGT]GC[ACT]^C BseRI 428 CTCCTC GAGGAG(10/8) BsmAI 943 GAGAC GTCTC(1/5) BsmFI 824 GTCCC GGGAC(10/14) Bsp1286I 1124 GGGCAC G[AGT]GC[ACT]^C Bsp1407I 151 TGTACA T^GTACA BspLU11I 180 ACATGT A^CATGT BsrGI 151 TGTACA T^GTACA BstUI 549 CGCG CG^CG EcoNI 810 CCTTTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 618 CCAAGG C^C[AT][AT]GG HapII 24 CCGG C^CGG HgaI 581 GACGC GACGC(5/10) HpaII 24 CCGG C^CGG HphI 8 TCACC GGTGA(8/7) MaeII 441 ACGT A^CGT MfeI 719 CAATTG C^AATTG MluNI 120 TGGCCA TGG^CCA MscI 120 TGGCCA TGG^CCA MspI 24 CCGG C^CGG MunI 719 CAATTG C^AATTG MvnI 549 CGCG CG^CG NciI 24 CCGGG CC^[GC]GG NdeI 1168 CATATG CA^TATG NheI 787 GCTAGC G^CTAGC NspI 180 ACATGT [GA]CATG^[CT] PflMI 729 CCATCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 725 GAGTC GAGTC(4/5) ScaI 424 AGTACT AGT^ACT SspBI 151 TGTACA T^GTACA SspI 466 AATATT AAT^ATT StyI 618 CCAAGG C^C[AT][AT]GG Van91I 729 CCATCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 1039 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AciI 77 CCGC CCGC(-3/-1) 550 GCGG BamHI 413 GGATCC G^GATCC 879 GGATCC BbsI 994 GAAGAC GAAGAC(2/6) 443 GTCTTC BpuAI 994 GAAGAC GAAGAC(2/6) 443 GTCTTC BstNI 850 CCTGG CC^[AT]GG 1099 CCTGG BstYI 413 GGATCC [GA]^GATC[CT] 879 GGATCC Cac8I 255 GCTTGC GC[ACGT]^[ACGT]GC 787 GCTAGC DdeI 19 CTCAG C^T[ACGT]AG 1055 CTCAG EcoRII 850 CCTGG ^CC[AT]GG 1099 CCTGG MflI 413 GGATCC [GA]^GATC[CT] 879 GGATCC MvaI 850 CCTGG CC^[AT]GG 1099 CCTGG SfcI 299 CTACAG C^T[GA][CT]AG 1049 CTGTAG TaqI 89 TCGA T^CGA 177 TCGA TspRI 302 CAGTG CA[GC]TG[ACGT][ACGT]^ 861 CACTG TthHB8I 89 TCGA T^CGA 177 TCGA XhoII 413 GGATCC [GA]^GATC[CT] 879 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BbvI BfaI BsaJI BsiYI BslI BsrI BssKI BstF5I DpnII EaeI Fnu4HI FokI HaeIII HinfI ItaI MaeI MaeIII MboI MboII MnlI MseI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI SfaNI TfiI Tru9I TseI Tsp45I Tsp509I