(Circular) (Six-base) MAPSORT of: pFN21AE6359.seq Check: 7857 from: 1 to: 5890 pFN21AE6359 5890 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3700 278 Size: 53 83 186 936 2164 2468 Fragments arranged by size: 2468 2164 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2849 3233 1937 Size: 912 384 4594 Fragments arranged by size: 4594 912 384 Acc65I G'GTAC_C Cuts at: 3211 3211 Size: 5890 AclI AA'CG_TT Cuts at: 1563 4017 4390 1563 Size: 2454 373 3063 Fragments arranged by size: 3063 2454 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2550 3953 5337 5605 1632 Size: 918 1403 1384 268 1917 Fragments arranged by size: 1917 1403 1384 918 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5693 AflIII A'CryG_T Cuts at: 1203 2185 4789 5766 1203 Size: 982 2604 977 1327 Fragments arranged by size: 2604 1327 982 977 AgeI A'CCGG_T Cuts at: 4721 4721 Size: 5890 AhdI GACnn_n'nnGTC Cuts at: 4619 4619 Size: 5890 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5890 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5890 AlwNI CAG_nnn'CTG Cuts at: 3633 5205 3633 Size: 1572 4318 ApaLI G'TGCA_C Cuts at: 3947 5103 3947 Size: 1156 4734 ApoI r'AATT_y Cuts at: 1493 2392 2516 2768 3199 3425 3577 5686 1493 Size: 899 124 252 431 226 152 2109 1697 Fragments arranged by size: 2109 1697 899 431 252 226 152 124 AseI AT'TA_AT Cuts at: 160 1032 4444 160 Size: 872 3412 1606 Fragments arranged by size: 3412 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5890 AvaI C'yCGr_G Cuts at: 1870 1943 3215 1870 Size: 73 1272 4545 Fragments arranged by size: 4545 1272 73 BamHI G'GATC_C Cuts at: 2418 2884 3220 3587 2418 Size: 466 336 367 4721 Fragments arranged by size: 4721 466 367 336 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3211 4666 618 Size: 325 217 627 1424 1455 1842 Fragments arranged by size: 1842 1455 1424 627 325 217 BanII G_rGCy'C Cuts at: 1391 3209 1391 Size: 1818 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2441 3006 928 Size: 971 542 565 3812 Fragments arranged by size: 3812 971 565 542 BbvCI CC'TCA_GC Cuts at: 2024 2024 Size: 5890 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4081 4115 1903 Size: 34 2144 34 3678 Fragments arranged by size: 3678 2144 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3782 4992 3782 Size: 1210 4680 BclI T'GATC_A Cuts at: 1548 1548 Size: 5890 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4501 136 Size: 107 122 71 631 605 2829 1525 Fragments arranged by size: 2829 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5885 5885 Size: 5890 BlpI GC'TnA_GC Cuts at: 3307 3307 Size: 5890 Bme1580I G_kGCm'C Cuts at: 1790 3133 3951 5107 1790 Size: 1343 818 1156 2573 Fragments arranged by size: 2573 1343 1156 818 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5890 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2149 2421 3621 Size: 416 53 83 399 24 732 272 1200 Cuts at: 3621 4579 442 Size: 958 1753 Fragments arranged by size: 1753 1200 958 732 416 399 272 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2796 1056 Size: 1740 4150 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4550 1239 Size: 3311 2579 Bpu10I CC'TnA_GC Cuts at: 2024 2024 Size: 5890 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4015 4880 5178 4015 Size: 865 298 4727 Fragments arranged by size: 4727 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4553 882 Size: 617 3054 2219 Fragments arranged by size: 3054 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3681 493 Size: 711 2477 2702 Fragments arranged by size: 2702 2477 711 BsaBI GATnn'nnATC Cuts at: 5884 5884 Size: 5890 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3697 4079 4714 275 Size: 53 83 186 936 2164 382 635 1451 Fragments arranged by size: 2164 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4323 4721 4995 5142 5553 4323 Size: 398 274 147 411 4660 Fragments arranged by size: 4660 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2337 2367 1178 Size: 30 165 30 934 30 4701 Fragments arranged by size: 4701 934 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2424 1186 Size: 1238 4652 BseYI C'CCAG_C Cuts at: 2788 5093 5589 2788 Size: 2305 496 3089 Fragments arranged by size: 3089 2305 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5317 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3340 4101 5129 1540 Size: 275 186 1339 761 1028 2301 Fragments arranged by size: 2301 1339 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3951 4036 5107 3951 Size: 85 1071 4734 Fragments arranged by size: 4734 1071 85 BsmI GAATG_Cn' Cuts at: 3401 3494 3401 Size: 93 5797 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3133 3209 3951 4036 5107 1391 Size: 399 1343 76 742 85 1071 2174 Fragments arranged by size: 2174 1343 1071 742 399 85 76 BspEI T'CCGG_A Cuts at: 5553 5553 Size: 5890 BspHI T'CATG_A Cuts at: 3779 5509 3779 Size: 1730 4160 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3245 844 Size: 2401 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3777 1394 Size: 429 1954 3507 Fragments arranged by size: 3507 1954 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4385 4559 65 Size: 1183 3137 174 1396 Fragments arranged by size: 3137 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4534 4721 1700 Size: 110 2724 187 2869 Fragments arranged by size: 2869 2724 187 110 BsrGI T'GTAC_A Cuts at: 96 2156 96 Size: 2060 3830 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5890 BssSI C'ACGA_G Cuts at: 3950 4962 3950 Size: 1012 4878 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5890 BstBI TT'CG_AA Cuts at: 4694 4694 Size: 5890 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5890 BstYI r'GATC_y Cuts at: 1972 2418 2884 3220 3587 3972 3989 5441 5527 Size: 446 466 336 367 385 17 1452 86 Cuts at: 5527 5539 5885 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 466 446 385 367 346 336 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4399 Fragments arranged by size: 4399 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2846 487 Size: 683 56 1620 3531 Fragments arranged by size: 3531 1620 683 56 BtsI GCAGTG_nn' Cuts at: 3406 4200 4220 3406 Size: 794 20 5076 Fragments arranged by size: 5076 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5890 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5855 DraI TTT'AAA Cuts at: 3194 3194 Size: 5890 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4897 809 Size: 507 3581 1802 Fragments arranged by size: 3581 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2079 2125 Size: 54 1425 50 275 18 177 72 46 Cuts at: 2125 3337 4226 5855 8 Size: 1212 889 1629 43 Fragments arranged by size: 1629 1425 1212 889 275 177 72 54 50 46 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3337 1537 Size: 275 1525 4090 Fragments arranged by size: 4090 1525 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3820 1353 Size: 2467 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4477 4851 4997 387 Size: 1064 138 2888 374 146 1280 Fragments arranged by size: 2888 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2550 3953 4550 5337 5605 1239 Size: 393 918 1403 597 787 268 1524 Fragments arranged by size: 1524 1403 918 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2819 2819 Size: 5890 EcoRI G'AATT_C Cuts at: 3199 3577 3199 Size: 378 5512 FspI TGC'GCA Cuts at: 4396 4396 Size: 5890 HaeII r_GCGC'y Cuts at: 1678 5037 1678 Size: 3359 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2687 2719 4531 4563 4605 Size: 32 135 32 1115 32 1812 32 42 Cuts at: 4605 4637 1373 Size: 32 2626 Fragments arranged by size: 2626 1812 1115 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3234 3480 669 Size: 988 281 1296 246 3079 Fragments arranged by size: 3079 1296 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5890 HpaI GTT'AAC Cuts at: 3480 3480 Size: 5890 KpnI G_GTAC'C Cuts at: 3215 3215 Size: 5890 MfeI C'AATT_G Cuts at: 2724 3489 2724 Size: 765 5125 MluI A'CGCG_T Cuts at: 5766 5766 Size: 5890 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5004 5188 1305 Size: 3699 184 2007 Fragments arranged by size: 3699 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2127 10 Size: 54 1425 343 295 3773 Fragments arranged by size: 3773 1425 343 295 54 MslI CAynn'nnrTG Cuts at: 518 1166 3848 4207 4366 518 Size: 648 2682 359 159 2042 Fragments arranged by size: 2682 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3306 3983 5131 5376 1606 Size: 1700 677 1148 245 2120 Fragments arranged by size: 2120 1700 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4399 Fragments arranged by size: 4399 939 552 NdeI CA'TA_TG Cuts at: 387 3174 387 Size: 2787 3103 NheI G'CTAG_C Cuts at: 1052 2792 1052 Size: 1740 4150 NotI GC'GGCC_GC Cuts at: 3337 3337 Size: 5890 NspI r_CATG'y Cuts at: 1132 1229 2189 3248 4793 1132 Size: 97 960 1059 1545 2229 Fragments arranged by size: 2229 1545 1059 960 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5890 PciI A'CATG_T Cuts at: 2185 4789 2185 Size: 2604 3286 PflMI CCAn_nnn'nTGG Cuts at: 1108 2740 1108 Size: 1632 4258 PfoI T'CCnGG_A Cuts at: 2854 2854 Size: 5890 PmeI GTTT'AAAC Cuts at: 3194 3194 Size: 5890 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3959 3991 5472 5504 3959 Size: 32 1481 32 4345 Fragments arranged by size: 4345 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5890 PsiI TTA'TAA Cuts at: 3460 3460 Size: 5890 PstI C_TGCA'G Cuts at: 830 3242 830 Size: 2412 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5890 SalI G'TCGA_C Cuts at: 1936 3232 1936 Size: 1296 4594 SbfI CC_TGCA'GG Cuts at: 3242 3242 Size: 5890 ScaI AGT'ACT Cuts at: 2431 4138 5850 2431 Size: 1707 1712 2471 Fragments arranged by size: 2471 1712 1707 SfcI C'TryA_G Cuts at: 826 1045 2304 3054 3238 4373 5054 5245 826 Size: 219 1259 750 184 1135 681 191 1471 Fragments arranged by size: 1471 1259 1135 750 681 219 191 184 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5890 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5890 SmaI CCC'GGG Cuts at: 1872 3217 1872 Size: 1345 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3994 4895 5157 820 Size: 197 926 2051 901 262 1553 Fragments arranged by size: 2051 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3681 493 Size: 3188 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5890 SphI G_CATG'C Cuts at: 1132 3248 1132 Size: 2116 3774 SspI AAT'ATT Cuts at: 5 52 2473 3814 5 Size: 47 2421 1341 2081 Fragments arranged by size: 2421 2081 1341 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5890 StyI C'CwwG_G Cuts at: 513 1065 2004 2623 3329 513 Size: 552 939 619 706 3074 Fragments arranged by size: 3074 939 706 619 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2427 3917 3934 4087 4272 4717 325 Size: 1255 847 1490 17 153 185 445 1498 Fragments arranged by size: 1498 1490 1255 847 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2156 2429 2896 Size: 274 80 33 51 1443 179 273 467 Cuts at: 2896 4136 5848 96 Size: 1240 1712 138 Fragments arranged by size: 1712 1443 1240 467 274 273 179 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2337 2369 5850 5882 2337 Size: 32 3481 32 2345 Fragments arranged by size: 3481 2345 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5890 XbaI T'CTAG_A Cuts at: 3226 3226 Size: 5890 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5890 XmaI C'CCGG_G Cuts at: 1870 3215 1870 Size: 1345 4545 XmnI GAAnn'nnTTC Cuts at: 3048 4019 3048 Size: 971 4919 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3698 276 Size: 53 83 186 936 2164 2468 Fragments arranged by size: 2468 2164 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI