################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaHI BsaI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MflI MluI MluNI MscI MspA1I MvaI NaeI NarI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccI 326 GTCGAC GT^[AC][GT]AC AccII 12 CGCG CG^CG AccIII 431 TCCGGA T^CCGGA AcsI 295 AAATTT [GA]^AATT[CT] AfaI 437 GTAC GT^AC AflIII 679 ACACGT A^C[GA][CT]GT AlwI 3 GGATC GGATC(4/5) ApoI 295 AAATTT [GA]^AATT[CT] AvaII 54 GGACC G^G[AT]CC BbrPI 680 CACGTG CAC^GTG BbvCI 57 CCTCAGC CCTCAGC(-5/-2) BbvI 8 GCAGC GCAGC(8/12) BcnI 171 CCGGG CC^[GC]GG BfaI 742 CTAG C^TAG BglI 62 GCCGGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 683 GTGCCC G[AGT]GC[ACT]^C BsaAI 680 CACGTG [CT]AC^GT[GA] BsaBI 733 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 431 TCCGGA [AT]^CCGG[AT] BseAI 431 TCCGGA T^CCGGA BsiYI 165 CCTTGACCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 165 CCTTGACCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 354 GCATTC GAATGC(1/-1) Bsp1286I 683 GTGCCC G[AGT]GC[ACT]^C BspEI 431 TCCGGA T^CCGGA BsrDI 36 GCAATG GCAATG(2/0) BssKI 171 CCGGG ^CC[ACGT]GG BstUI 12 CGCG CG^CG Cfr13I 54 GGACC G^G[ACGT]CC DsaI 359 CCATGG C^C[GA][CT]GG EaeI 150 TGGCCG [CT]^GGCC[GA] EcoT14I 359 CCATGG C^C[AT][AT]GG HaeIII 151 GGCC GG^CC MaeI 742 CTAG C^TAG MaeII 681 ACGT A^CGT MamI 733 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 331 CAATTG C^AATTG MroI 431 TCCGGA T^CCGGA MslI 393 CATAACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 331 CAATTG C^AATTG MvnI 12 CGCG CG^CG NciI 171 CCGGG CC^[GC]GG NcoI 359 CCATGG C^CATGG PmaCI 680 CACGTG CAC^GTG PmlI 680 CACGTG CAC^GTG RsaI 437 GTAC GT^AC SalI 326 GTCGAC G^TCGAC Sau96I 54 GGACC G^G[ACGT]CC ScrFI 171 CCGGG CC^[ACGT]GG SfcI 574 CTACAG C^T[GA][CT]AG SphI 579 GCATGC GCATG^C StyI 359 CCATGG C^C[AT][AT]GG TaqI 327 TCGA T^CGA TseI 8 GCAGC G^C[AT]GC Tsp45I 484 GTGAC ^GT[GC]AC TthHB8I 327 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BsaJI 171 CCGGGG C^C[ACGT][ACGT]GG 359 CCATGG BsgI 100 GTGCAG GTGCAG(16/14) 319 GTGCAG BsrI 219 ACTGG ACTGG(1/-1) 687 CCAGT DdeI 58 CTCAG C^T[ACGT]AG 447 CTTAG Fnu4HI 8 GCAGC GC^[ACGT]GC 152 GCCGC HincII 76 GTTGAC GT[CT]^[GA]AC 326 GTCGAC HindII 76 GTTGAC GT[CT]^[GA]AC 326 GTCGAC ItaI 8 GCAGC GC^[ACGT]GC 152 GCCGC MseI 460 TTAA T^TAA 511 TTAA MwoI 62 GCCGGAAGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 146 GCGCTGGCCGC NspI 113 ACATGC [GA]CATG^[CT] 579 GCATGC Tru9I 460 TTAA T^TAA 511 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AciI AluI AlwNI BstF5I Cac8I CfoI DpnII FokI HapII HhaI HinP1I HinfI HpaII HphI MaeIII MboI MboII MnlI MspI NdeII NlaIII Sau3AI SfaNI TfiI Tsp509I TspRI