(Linear) (Six-base) MAPSORT of: KIEE2228.seq  Check: 6458  from: 1  to: 777



KIEE2228.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:56  ..

AccI GT'mk_AC

Cuts at:      0     327     777 
   Size:        327     450
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286     777 
   Size:        286     491
 
AflIII A'CryG_T

Cuts at:      0     679     777 
   Size:        679      98
 
AlwNI CAG_nnn'CTG

Cuts at:      0     108     143     351     777 
   Size:        108      35     208     426

  Fragments arranged by size: 

                 426     208     108      35
 
ApoI r'AATT_y

Cuts at:      0     295     777 
   Size:        295     482
 
BbvCI CC'TCA_GC

Cuts at:      0      58     777 
   Size:         58     719
 
BglI GCCn_nnn'nGGC

Cuts at:      0      68     777 
   Size:         68     709
 
Bme1580I G_kGCm'C

Cuts at:      0     687     777 
   Size:        687      90
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     681     777 
   Size:        681      96
 
Bpu10I CC'TnA_GC

Cuts at:      0      58     777 
   Size:         58     719
 
BsaAI yAC'GTr

Cuts at:      0     682     777 
   Size:        682      95
 
BsaBI GATnn'nnATC

Cuts at:      0     737     777 
   Size:        737      40
 
BsaWI w'CCGG_w

Cuts at:      0     431     777 
   Size:        431     346
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     340     777 
   Size:        121     219     437

  Fragments arranged by size: 

                 437     219     121
 
BsmI GAATG_Cn'

Cuts at:      0     354     777 
   Size:        354     423
 
Bsp1286I G_dGCh'C

Cuts at:      0     687     777 
   Size:        687      90
 
BspEI T'CCGG_A

Cuts at:      0     431     777 
   Size:        431     346
 
BsrDI GCAATG_nn'

Cuts at:      0      43     777 
   Size:         43     734
 
BtgI C'CryG_G

Cuts at:      0     359     777 
   Size:        359     418
 
BtsI GCAGTG_nn'

Cuts at:      0      78     328     777 
   Size:         78     250     449

  Fragments arranged by size: 

                 449     250      78
 
EaeI y'GGCC_r

Cuts at:      0     150     777 
   Size:        150     627
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286     777 
   Size:        286     491
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     246     278     777 
   Size:        246      32     499

  Fragments arranged by size: 

                 499     246      32
 
HincII GTy'rAC

Cuts at:      0      78     328     777 
   Size:         78     250     449

  Fragments arranged by size: 

                 449     250      78
 
MfeI C'AATT_G

Cuts at:      0     331     777 
   Size:        331     446
 
MslI CAynn'nnrTG

Cuts at:      0     397     777 
   Size:        397     380
 
NcoI C'CATG_G

Cuts at:      0     359     777 
   Size:        359     418
 
NspI r_CATG'y

Cuts at:      0     117     583     777 
   Size:        117     466     194

  Fragments arranged by size: 

                 466     194     117
 
PmlI CAC'GTG

Cuts at:      0     682     777 
   Size:        682      95
 
SalI G'TCGA_C

Cuts at:      0     326     777 
   Size:        326     451
 
SfcI C'TryA_G

Cuts at:      0     574     777 
   Size:        574     203
 
SphI G_CATG'C

Cuts at:      0     583     777 
   Size:        583     194
 
StyI C'CwwG_G

Cuts at:      0     359     777 
   Size:        359     418
 
TatI w'GTAC_w

Cuts at:      0     436     777 
   Size:        436     341
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      57      89     777 
   Size:         57      32     688

  Fragments arranged by size: 

                 688      57      32
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     ApoI    BbvCI     BglI Bme1580I
     BmrI   Bpu10I    BsaAI    BsaBI    BsaWI     BsgI     BsmI Bsp1286I
    BspEI    BsrDI     BtgI     BtsI     EaeI  Eco57MI    Hin4I   HincII
     MfeI     MslI     NcoI     NspI     PmlI     SalI     SfcI     SphI
     StyI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaHI    BsaXI    BseRI    BseYI
    BsiEI  BsiHKAI    BsiWI    BsmBI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI
     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI