(Circular) (Six-base) MAPSORT of: pFN21AE3328.seq Check: 7547 from: 1 to: 7513 pFN21AE3328 7513 bp With 182 enzymes: SgfI * March 28, 2012 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5323 278 Size: 53 83 186 936 3787 2468 Fragments arranged by size: 3787 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4856 1937 Size: 2919 4594 Acc65I G'GTAC_C Cuts at: 4834 4834 Size: 7513 AclI AA'CG_TT Cuts at: 1563 3778 5640 6013 1563 Size: 2215 1862 373 3063 Fragments arranged by size: 3063 2215 1862 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2455 3202 3530 3665 4237 4446 4467 5576 Size: 823 747 328 135 572 209 21 1109 Cuts at: 5576 6960 7228 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1109 823 747 572 328 268 209 135 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7316 AflIII A'CryG_T Cuts at: 1203 3458 6412 7389 1203 Size: 2255 2954 977 1327 Fragments arranged by size: 2954 2255 1327 977 AgeI A'CCGG_T Cuts at: 6344 6344 Size: 7513 AhdI GACnn_n'nnGTC Cuts at: 6242 6242 Size: 7513 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7513 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7513 AlwNI CAG_nnn'CTG Cuts at: 2138 4153 4322 5256 6828 2138 Size: 2015 169 934 1572 2823 Fragments arranged by size: 2823 2015 1572 934 169 ApaLI G'TGCA_C Cuts at: 4222 5570 6726 4222 Size: 1348 1156 5009 Fragments arranged by size: 5009 1348 1156 ApoI r'AATT_y Cuts at: 1493 2108 2317 2870 3152 3275 4278 4293 4439 Size: 615 209 553 282 123 1003 15 146 Cuts at: 4439 4822 5048 5200 7309 1493 Size: 383 226 152 2109 1697 Fragments arranged by size: 2109 1697 1003 615 553 383 282 226 209 152 146 123 15 AseI AT'TA_AT Cuts at: 160 1032 2336 3998 6067 160 Size: 872 1304 1662 2069 1606 Fragments arranged by size: 2069 1662 1606 1304 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7513 AvaI C'yCGr_G Cuts at: 1870 1943 4838 1870 Size: 73 2895 4545 Fragments arranged by size: 4545 2895 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4394 4427 4394 Size: 33 7480 BamHI G'GATC_C Cuts at: 2233 4843 5210 2233 Size: 2610 367 4536 Fragments arranged by size: 4536 2610 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4834 6289 618 Size: 325 217 627 3047 1455 1842 Fragments arranged by size: 3047 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2583 4763 4832 1391 Size: 1192 2180 69 4072 Fragments arranged by size: 4072 2180 1192 69 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3015 4167 4243 4438 4703 928 Size: 971 1116 1152 76 195 265 3738 Fragments arranged by size: 3738 1152 1116 971 265 195 76 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4208 4242 5704 5738 1903 Size: 34 2271 34 1462 34 3678 Fragments arranged by size: 3678 2271 1462 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2693 4756 5405 6615 2693 Size: 2063 649 1210 3591 Fragments arranged by size: 3591 2063 1210 649 BclI T'GATC_A Cuts at: 1548 2655 3286 1548 Size: 1107 631 5775 Fragments arranged by size: 5775 1107 631 BfrBI ATG'CAT Cuts at: 4768 4768 Size: 7513 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6124 136 Size: 107 122 71 631 605 4452 1525 Fragments arranged by size: 4452 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7508 7508 Size: 7513 BlpI GC'TnA_GC Cuts at: 4930 4930 Size: 7513 Bme1580I G_kGCm'C Cuts at: 1790 2281 3330 4226 5574 6730 1790 Size: 491 1049 896 1348 1156 2573 Fragments arranged by size: 2573 1348 1156 1049 896 491 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7513 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5244 6202 442 Size: 416 53 83 399 24 3827 958 1753 Fragments arranged by size: 3827 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7513 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2262 3585 6173 1239 Size: 1023 1323 2588 2579 Fragments arranged by size: 2588 2579 1323 1023 Bpu10I CC'TnA_GC Cuts at: 3718 3718 Size: 7513 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2982 4295 4662 5638 6503 6801 2982 Size: 1313 367 976 865 298 3694 Fragments arranged by size: 3694 1313 976 865 367 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3517 4527 6176 882 Size: 617 2018 1010 1649 2219 Fragments arranged by size: 2219 2018 1649 1010 617 BsaAI yAC'GTr Cuts at: 493 1204 2102 5304 493 Size: 711 898 3202 2702 Fragments arranged by size: 3202 2702 898 711 BsaBI GATnn'nnATC Cuts at: 3405 7507 3405 Size: 4102 3411 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5320 5702 6337 275 Size: 53 83 186 936 3787 382 635 1451 Fragments arranged by size: 3787 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2026 5946 6344 6618 6765 7176 2026 Size: 3920 398 274 147 411 2363 Fragments arranged by size: 3920 2363 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2769 2799 1178 Size: 30 165 30 1366 30 5892 Fragments arranged by size: 5892 1366 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3582 3635 4709 1186 Size: 2396 53 1074 3990 Fragments arranged by size: 3990 2396 1074 53 BseYI C'CCAG_C Cuts at: 2052 3425 3902 6716 7212 2052 Size: 1373 477 2814 496 2353 Fragments arranged by size: 2814 2353 1373 496 477 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6940 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4963 5724 6752 1540 Size: 275 186 2962 761 1028 2301 Fragments arranged by size: 2962 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4226 4763 5574 5659 6730 4226 Size: 537 811 85 1071 5009 Fragments arranged by size: 5009 1071 811 537 85 BsmI GAATG_Cn' Cuts at: 2998 5024 5117 2998 Size: 2026 93 5394 Fragments arranged by size: 5394 2026 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2281 2583 3330 4226 4763 4832 5574 Size: 399 491 302 747 896 537 69 742 Cuts at: 5574 5659 6730 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 896 747 742 537 491 399 302 85 69 BspEI T'CCGG_A Cuts at: 2026 7176 2026 Size: 5150 2363 BspHI T'CATG_A Cuts at: 3961 4455 5402 7132 3961 Size: 494 947 1730 4342 Fragments arranged by size: 4342 1730 947 494 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2418 2683 3420 4868 844 Size: 1574 265 737 1448 3489 Fragments arranged by size: 3489 1574 1448 737 265 BsrBI CCG'CTC Cuts at: 1394 1823 5400 1394 Size: 429 3577 3507 Fragments arranged by size: 3577 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2816 6008 6182 65 Size: 1183 1568 3192 174 1396 Fragments arranged by size: 3192 1568 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6157 6344 1700 Size: 110 4347 187 2869 Fragments arranged by size: 4347 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7513 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7513 BssSI C'ACGA_G Cuts at: 5573 6585 5573 Size: 1012 6501 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7513 BstBI TT'CG_AA Cuts at: 2560 4608 6317 2560 Size: 2048 1709 3756 Fragments arranged by size: 3756 2048 1709 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7513 BstXI CCAn_nnnn'nTGG Cuts at: 4027 4661 4027 Size: 634 6879 BstYI r'GATC_y Cuts at: 1972 2233 4843 5210 5595 5612 7064 7150 7162 Size: 261 2610 367 385 17 1452 86 12 Cuts at: 7162 7508 1972 Size: 346 1977 Fragments arranged by size: 2610 1977 1452 385 367 346 261 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4206 4206 Size: 7513 BtgI C'CryG_G Cuts at: 513 1065 2004 3355 513 Size: 552 939 1351 4671 Fragments arranged by size: 4671 1351 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6774 Fragments arranged by size: 6774 683 56 BtsI GCAGTG_nn' Cuts at: 2712 4226 4522 5029 5823 5843 2712 Size: 1514 296 507 794 20 4382 Fragments arranged by size: 4382 1514 794 507 296 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7513 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7478 DraI TTT'AAA Cuts at: 2487 4817 2487 Size: 2330 5183 DraIII CAC_nnn'GTG Cuts at: 2276 4221 2276 Size: 1945 5568 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6520 809 Size: 507 5204 1802 Fragments arranged by size: 5204 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2740 4573 4960 Size: 54 1425 50 275 18 910 1833 387 Cuts at: 4960 5849 7478 8 Size: 889 1629 43 Fragments arranged by size: 1833 1629 1425 910 889 387 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4960 1537 Size: 275 3148 4090 Fragments arranged by size: 4090 3148 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3965 4461 5443 1353 Size: 2612 496 982 3423 Fragments arranged by size: 3423 2612 982 496 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2573 6100 6474 6620 387 Size: 1064 138 984 3527 374 146 1280 Fragments arranged by size: 3527 1280 1064 984 374 146 138 EcoICRI GAG'CTC Cuts at: 4761 4761 Size: 7513 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2262 2455 3202 3530 3585 3665 4237 Size: 393 630 193 747 328 55 80 572 Cuts at: 4237 4446 4467 5576 6173 6960 7228 1239 Size: 209 21 1109 597 787 268 1524 Fragments arranged by size: 1524 1109 787 747 630 597 572 393 328 268 209 193 80 55 21 EcoO109I rG'GnC_Cy Cuts at: 2020 4568 4635 2020 Size: 2548 67 4898 Fragments arranged by size: 4898 2548 67 EcoRI G'AATT_C Cuts at: 2108 2317 4822 5200 2108 Size: 209 2505 378 4421 Fragments arranged by size: 4421 2505 378 209 FspI TGC'GCA Cuts at: 6019 6019 Size: 7513 HaeII r_GCGC'y Cuts at: 1678 6660 1678 Size: 4982 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2647 2679 2769 2801 3974 Size: 32 135 32 1075 32 90 32 1173 Cuts at: 3974 4006 4478 4510 6154 6186 6228 6260 1373 Size: 32 472 32 1644 32 42 32 2626 Fragments arranged by size: 2626 1644 1173 1075 472 135 90 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4857 5103 669 Size: 988 281 2919 246 3079 Fragments arranged by size: 3079 2919 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7513 HpaI GTT'AAC Cuts at: 5103 5103 Size: 7513 KpnI G_GTAC'C Cuts at: 4838 4838 Size: 7513 MfeI C'AATT_G Cuts at: 5112 5112 Size: 7513 MluI A'CGCG_T Cuts at: 7389 7389 Size: 7513 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2677 2884 4175 6627 6811 1305 Size: 1372 207 1291 2452 184 2007 Fragments arranged by size: 2452 2007 1372 1291 207 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2742 10 Size: 54 1425 343 910 4781 Fragments arranged by size: 4781 1425 910 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2346 5471 5830 5989 518 Size: 648 1180 3125 359 159 2042 Fragments arranged by size: 3125 2042 1180 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2012 2135 2173 4319 4929 5606 6754 6999 Size: 406 123 38 2146 610 677 1148 245 Cuts at: 6999 1606 Size: 2120 Fragments arranged by size: 2146 2120 1148 677 610 406 245 123 38 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6022 Fragments arranged by size: 6022 939 552 NdeI CA'TA_TG Cuts at: 387 2377 387 Size: 1990 5523 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7513 NotI GC'GGCC_GC Cuts at: 4960 4960 Size: 7513 NsiI A_TGCA'T Cuts at: 4770 4770 Size: 7513 NspI r_CATG'y Cuts at: 1132 1229 3141 3462 4112 4422 4871 6416 1132 Size: 97 1912 321 650 310 449 1545 2229 Fragments arranged by size: 2229 1912 1545 650 449 321 310 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7513 PciI A'CATG_T Cuts at: 3458 6412 3458 Size: 2954 4559 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7513 PmeI GTTT'AAAC Cuts at: 4817 4817 Size: 7513 PmlI CAC'GTG Cuts at: 2102 2102 Size: 7513 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5582 5614 7095 7127 5582 Size: 32 1481 32 5968 Fragments arranged by size: 5968 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2020 4568 4635 2020 Size: 2548 67 4898 Fragments arranged by size: 4898 2548 67 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7513 PsiI TTA'TAA Cuts at: 3519 5083 3519 Size: 1564 5949 PstI C_TGCA'G Cuts at: 830 2415 4865 830 Size: 1585 2450 3478 Fragments arranged by size: 3478 2450 1585 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7513 PvuII CAG'CTG Cuts at: 2012 2173 2012 Size: 161 7352 SacI G_AGCT'C Cuts at: 4763 4763 Size: 7513 SalI G'TCGA_C Cuts at: 1936 4855 1936 Size: 2919 4594 SanDI GG'GwC_CC Cuts at: 2020 2020 Size: 7513 SbfI CC_TGCA'GG Cuts at: 4865 4865 Size: 7513 ScaI AGT'ACT Cuts at: 5761 7473 5761 Size: 1712 5801 SfcI C'TryA_G Cuts at: 826 1045 2411 4145 4861 5996 6677 6868 826 Size: 219 1366 1734 716 1135 681 191 1471 Fragments arranged by size: 1734 1471 1366 1135 716 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7513 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7513 SmaI CCC'GGG Cuts at: 1872 4840 1872 Size: 2968 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2997 4310 4677 5617 6518 6780 Size: 197 926 1054 1313 367 940 901 262 Cuts at: 6780 820 Size: 1553 Fragments arranged by size: 1553 1313 1054 940 926 901 367 262 197 SnaBI TAC'GTA Cuts at: 493 5304 493 Size: 4811 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7513 SphI G_CATG'C Cuts at: 1132 3141 4112 4871 1132 Size: 2009 971 759 3774 Fragments arranged by size: 3774 2009 971 759 SspI AAT'ATT Cuts at: 5 52 2791 2950 3554 4087 4670 5437 5 Size: 47 2739 159 604 533 583 767 2081 Fragments arranged by size: 2739 2081 767 604 583 533 159 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7513 StyI C'CwwG_G Cuts at: 513 1065 2004 2206 3165 3313 3600 4952 513 Size: 552 939 202 959 148 287 1352 3074 Fragments arranged by size: 3074 1352 959 939 552 287 202 148 SwaI ATTT'AAAT Cuts at: 2487 2487 Size: 7513 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2066 3872 4738 5540 5557 5710 5895 Size: 1255 486 1806 866 802 17 153 185 Cuts at: 5895 6340 325 Size: 445 1498 Fragments arranged by size: 1806 1498 1255 866 802 486 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5759 7471 96 Size: 274 80 33 51 1443 3782 1712 138 Fragments arranged by size: 3782 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7473 7505 7473 Size: 32 7481 Tth111I GACn'n_nGTC Cuts at: 1316 3014 3269 1316 Size: 1698 255 5560 Fragments arranged by size: 5560 1698 255 XbaI T'CTAG_A Cuts at: 4849 4849 Size: 7513 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3320 3710 3811 3320 Size: 390 101 7022 Fragments arranged by size: 7022 390 101 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7513 XmaI C'CCGG_G Cuts at: 1870 4838 1870 Size: 2968 4545 XmnI GAAnn'nnTTC Cuts at: 5642 5642 Size: 7513 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5321 276 Size: 53 83 186 936 3787 2468 Fragments arranged by size: 3787 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI