Restriction map for
pFN21AE3328
(Linear) (Six-base) MAPSORT of: KIEE3328.seq Check: 8389 from: 1 to: 2808
KIEE3328.orf
With 182 enzymes: SgfI *
March 2, 2011 13:37 ..
AclI AA'CG_TT
Cuts at: 0 1773 2808
Size: 1773 1035
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 450 1197 1525 1660 2232 2441 2462 2808
Size: 450 747 328 135 572 209 21 346
Fragments arranged by size:
747 572 450 346 328 209 135 21
AflIII A'CryG_T
Cuts at: 0 1453 2808
Size: 1453 1355
AlwNI CAG_nnn'CTG
Cuts at: 0 133 2148 2317 2808
Size: 133 2015 169 491
Fragments arranged by size:
2015 491 169 133
ApaLI G'TGCA_C
Cuts at: 0 2217 2808
Size: 2217 591
ApoI r'AATT_y
Cuts at: 0 103 312 865 1147 1270 2273 2288 2434
Size: 103 209 553 282 123 1003 15 146
Cuts at: 2434 2808
Size: 374
Fragments arranged by size:
1003 553 374 282 209 146 123 103
15
AseI AT'TA_AT
Cuts at: 0 331 1993 2808
Size: 331 1662 815
Fragments arranged by size:
1662 815 331
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2389 2422 2808
Size: 2389 33 386
Fragments arranged by size:
2389 386 33
BamHI G'GATC_C
Cuts at: 0 228 2808
Size: 228 2580
BanII G_rGCy'C
Cuts at: 0 578 2758 2808
Size: 578 2180 50
Fragments arranged by size:
2180 578 50
BbsI GAAGACnn'nnnn_
Cuts at: 0 1010 2162 2238 2433 2698 2808
Size: 1010 1152 76 195 265 110
Fragments arranged by size:
1152 1010 265 195 110 76
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2203 2237 2808
Size: 2203 34 571
Fragments arranged by size:
2203 571 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 688 2751 2808
Size: 688 2063 57
Fragments arranged by size:
2063 688 57
BclI T'GATC_A
Cuts at: 0 650 1281 2808
Size: 650 631 1527
Fragments arranged by size:
1527 650 631
BfrBI ATG'CAT
Cuts at: 0 2763 2808
Size: 2763 45
Bme1580I G_kGCm'C
Cuts at: 0 276 1325 2221 2808
Size: 276 1049 896 587
Fragments arranged by size:
1049 896 587 276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 1580 2808
Size: 257 1323 1228
Fragments arranged by size:
1323 1228 257
Bpu10I CC'TnA_GC
Cuts at: 0 1713 2808
Size: 1713 1095
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 977 2290 2657 2808
Size: 977 1313 367 151
Fragments arranged by size:
1313 977 367 151
BsaI GGTCTCn'nnnn_
Cuts at: 0 1512 2522 2808
Size: 1512 1010 286
Fragments arranged by size:
1512 1010 286
BsaAI yAC'GTr
Cuts at: 0 97 2808
Size: 97 2711
BsaBI GATnn'nnATC
Cuts at: 0 1400 2808
Size: 1400 1408
BsaWI w'CCGG_w
Cuts at: 0 21 2808
Size: 21 2787
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 764 794 2808
Size: 764 30 2014
Fragments arranged by size:
2014 764 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1577 1630 2704 2808
Size: 1577 53 1074 104
Fragments arranged by size:
1577 1074 104 53
BseYI C'CCAG_C
Cuts at: 0 47 1420 1897 2808
Size: 47 1373 477 911
Fragments arranged by size:
1373 911 477 47
BsiHKAI G_wGCw'C
Cuts at: 0 2221 2758 2808
Size: 2221 537 50
Fragments arranged by size:
2221 537 50
BsmI GAATG_Cn'
Cuts at: 0 993 2808
Size: 993 1815
Bsp1286I G_dGCh'C
Cuts at: 0 276 578 1325 2221 2758 2808
Size: 276 302 747 896 537 50
Fragments arranged by size:
896 747 537 302 276 50
BspEI T'CCGG_A
Cuts at: 0 21 2808
Size: 21 2787
BspHI T'CATG_A
Cuts at: 0 1956 2450 2808
Size: 1956 494 358
Fragments arranged by size:
1956 494 358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 413 678 1415 2808
Size: 413 265 737 1393
Fragments arranged by size:
1393 737 413 265
BsrDI GCAATG_nn'
Cuts at: 0 811 2808
Size: 811 1997
BstBI TT'CG_AA
Cuts at: 0 555 2603 2808
Size: 555 2048 205
Fragments arranged by size:
2048 555 205
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2022 2656 2808
Size: 2022 634 152
Fragments arranged by size:
2022 634 152
BstYI r'GATC_y
Cuts at: 0 228 2808
Size: 228 2580
Bsu36I CC'TnA_GG
Cuts at: 0 2201 2808
Size: 2201 607
BtgI C'CryG_G
Cuts at: 0 1350 2808
Size: 1350 1458
BtsI GCAGTG_nn'
Cuts at: 0 707 2221 2517 2808
Size: 707 1514 296 291
Fragments arranged by size:
1514 707 296 291
DraI TTT'AAA
Cuts at: 0 482 2808
Size: 482 2326
DraIII CAC_nnn'GTG
Cuts at: 0 271 2216 2808
Size: 271 1945 592
Fragments arranged by size:
1945 592 271
EaeI y'GGCC_r
Cuts at: 0 735 2568 2808
Size: 735 1833 240
Fragments arranged by size:
1833 735 240
EarI CTCTTCn'nnn_
Cuts at: 0 1960 2456 2808
Size: 1960 496 352
Fragments arranged by size:
1960 496 352
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 568 2808
Size: 568 2240
EcoICRI GAG'CTC
Cuts at: 0 2756 2808
Size: 2756 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 450 1197 1525 1580 1660 2232 2441
Size: 257 193 747 328 55 80 572 209
Cuts at: 2441 2462 2808
Size: 21 346
Fragments arranged by size:
747 572 346 328 257 209 193 80
55 21
EcoO109I rG'GnC_Cy
Cuts at: 0 15 2563 2630 2808
Size: 15 2548 67 178
Fragments arranged by size:
2548 178 67 15
EcoRI G'AATT_C
Cuts at: 0 103 312 2808
Size: 103 209 2496
Fragments arranged by size:
2496 209 103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 642 674 764 796 1969 2001 2473 2505
Size: 642 32 90 32 1173 32 472 32
Cuts at: 2505 2808
Size: 303
Fragments arranged by size:
1173 642 472 303 90 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 672 879 2170 2808
Size: 672 207 1291 638
Fragments arranged by size:
1291 672 638 207
MscI TGG'CCA
Cuts at: 0 737 2808
Size: 737 2071
MslI CAynn'nnrTG
Cuts at: 0 341 2808
Size: 341 2467
MspA1I CmG'CkG
Cuts at: 0 7 130 168 2314 2808
Size: 7 123 38 2146 494
Fragments arranged by size:
2146 494 123 38 7
NdeI CA'TA_TG
Cuts at: 0 372 2808
Size: 372 2436
NsiI A_TGCA'T
Cuts at: 0 2765 2808
Size: 2765 43
NspI r_CATG'y
Cuts at: 0 1136 1457 2107 2417 2808
Size: 1136 321 650 310 391
Fragments arranged by size:
1136 650 391 321 310
PciI A'CATG_T
Cuts at: 0 1453 2808
Size: 1453 1355
PmlI CAC'GTG
Cuts at: 0 97 2808
Size: 97 2711
PpuMI rG'GwC_Cy
Cuts at: 0 15 2563 2630 2808
Size: 15 2548 67 178
Fragments arranged by size:
2548 178 67 15
PsiI TTA'TAA
Cuts at: 0 1514 2808
Size: 1514 1294
PstI C_TGCA'G
Cuts at: 0 410 2808
Size: 410 2398
PvuII CAG'CTG
Cuts at: 0 7 168 2808
Size: 7 161 2640
Fragments arranged by size:
2640 161 7
SacI G_AGCT'C
Cuts at: 0 2758 2808
Size: 2758 50
SanDI GG'GwC_CC
Cuts at: 0 15 2808
Size: 15 2793
SfcI C'TryA_G
Cuts at: 0 406 2140 2808
Size: 406 1734 668
Fragments arranged by size:
1734 668 406
SmlI C'TyrA_G
Cuts at: 0 992 2305 2672 2808
Size: 992 1313 367 136
Fragments arranged by size:
1313 992 367 136
SphI G_CATG'C
Cuts at: 0 1136 2107 2808
Size: 1136 971 701
Fragments arranged by size:
1136 971 701
SspI AAT'ATT
Cuts at: 0 786 945 1549 2082 2665 2808
Size: 786 159 604 533 583 143
Fragments arranged by size:
786 604 583 533 159 143
StyI C'CwwG_G
Cuts at: 0 201 1160 1308 1595 2808
Size: 201 959 148 287 1213
Fragments arranged by size:
1213 959 287 201 148
SwaI ATTT'AAAT
Cuts at: 0 482 2808
Size: 482 2326
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 61 1867 2733 2808
Size: 61 1806 866 75
Fragments arranged by size:
1806 866 75 61
Tth111I GACn'n_nGTC
Cuts at: 0 1009 1264 2808
Size: 1009 255 1544
Fragments arranged by size:
1544 1009 255
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1315 1705 1806 2808
Size: 1315 390 101 1002
Fragments arranged by size:
1315 1002 390 101
Enzymes that do cut:
AclI AcuI AflIII AlwNI ApaLI ApoI AseI BaeI
BamHI BanII BbsI BcgI BciVI BclI BfrBI Bme1580I
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrDI BstBI BstXI BstYI Bsu36I BtgI BtsI DraI
DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
Hin4I MmeI MscI MslI MspA1I NdeI NsiI NspI
PciI PmlI PpuMI PsiI PstI PvuII SacI SanDI
SfcI SmlI SphI SspI StyI SwaI TaqII Tth111I
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI AscI AsiSI AvaI AvrII
BanI BbeI BbvCI BglI BglII BlpI BmgBI BmrI
BmtI BplI BsaHI BsgI BsiEI BsiWI BsmBI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstEII BstZ17I BtgZI
ClaI CspCI DrdI EagI EcoNI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI
NruI PacI PasI PflMI PfoI PmeI PpiI PshAI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI StuI TatI TstI XbaI XhoI
XmaI XmnI ZraI