Restriction map for
pFN21AE3329
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# NO CUT ENZYMES ( 94 enzymes) #
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AatII Acc65I AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BbrPI
BbvCI BcgI BclI BfrI BlnI
BsaAI BsaBI BseRI BsiHKAI BsiWI
BspDI BspMI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraIII Eam1105I
Eco47III Eco81I EcoNI EcoO65I FbaI
FseI FspI HindIII HpaI KpnI
MamI MfeI MluI MunI NaeI
NdeI NgoMIV NheI NotI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
Sse8387I StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 2583 GTCGAC GT^[AC][GT]AC
AccIII 890 TCCGGA T^CCGGA
AlwNI 872 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1663 ATTAAT AT^TAAT
AsnI 1663 ATTAAT AT^TAAT
AvaI 2373 CCCGAG C^[CT]CG[GA]G
BamHI 2623 GGATCC G^GATCC
BbeI 992 GGCGCC GGCGC^C
BbsI 2143 GAAGAC GAAGAC(2/6)
BglI 303 GCCCCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 849 AGATCT A^GATCT
BlpI 1498 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1498 GCTCAGC GC^T[ACGT]AGC
BpuAI 2143 GAAGAC GAAGAC(2/6)
BsaI 2205 GAGACC GGTCTC(1/5)
BsaWI 890 TCCGGA [AT]^CCGG[AT]
BseAI 890 TCCGGA T^CCGGA
BsiEI 7 CGGCCG CG[GA][CT]^CG
BsmI 1368 GAATGC GAATGC(1/-1)
BsoBI 2373 CCCGAG C^[CT]CG[GA]G
BspEI 890 TCCGGA T^CCGGA
BsrBI 34 CCGCTC CCGCTC(-3/-3)
BsrFI 5 ACCGGC [GA]^CCGG[CT]
CelII 1498 GCTCAGC GC^T[ACGT]AGC
Cfr10I 5 ACCGGC [GA]^CCGG[CT]
DraI 2215 TTTAAA TTT^AAA
DrdI 2252 GACTCTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 7 CGGCCG C^GGCCG
EclXI 7 CGGCCG C^GGCCG
Eco52I 7 CGGCCG C^GGCCG
EcoT22I 1370 ATGCAT ATGCA^T
HincII 2583 GTCGAC GT[CT]^[GA]AC
HindII 2583 GTCGAC GT[CT]^[GA]AC
KasI 992 GGCGCC G^GCGCC
KspI 18 CCGCGG CCGC^GG
MroI 890 TCCGGA T^CCGGA
NarI 992 GGCGCC GG^CGCC
NruI 744 TCGCGA TCG^CGA
NsiI 1370 ATGCAT ATGCA^T
PshAI 736 GACTTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1663 ATTAAT AT^TAAT
SacII 18 CCGCGG CCGC^GG
SalI 2583 GTCGAC G^TCGAC
SfoI 992 GGCGCC GGC^GCC
SpeI 1221 ACTAGT A^CTAGT
SphI 1646 GCATGC GCATG^C
XcmI 2070 CCAGCCATATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcyI 992 GGCGCC G[GA]^CG[CT]C
2764 GACGCC
BanII 301 GAGCCC G[GA]GC[CT]^C
867 GAGCCC
BcnI 305 CCCGG CC^[GC]GG
963 CCGGG
BmyI 301 GAGCCC G[AGT]GC[ACT]^C
867 GAGCCC
BsaHI 992 GGCGCC G[GA]^CG[CT]C
2764 GACGCC
BsmBI 206 CGTCTC CGTCTC(1/5)
2762 GAGACG
Bsp1286I 301 GAGCCC G[AGT]GC[ACT]^C
867 GAGCCC
Bsp1407I 1214 TGTACA T^GTACA
2162 TGTACA
BspHI 1694 TCATGA T^CATGA
2171 TCATGA
BspLU11I 2366 ACATGT A^CATGT
2660 ACATGT
BsrGI 1214 TGTACA T^GTACA
2162 TGTACA
DraII 1841 AGGACCT [GA]G^G[ACGT]CC[CT]
2517 AGGTCCT
EarI 1261 CTCTTC CTCTTC(1/4)
2007 CTCTTC
EcoO109I 1841 AGGACCT [GA]G^G[ACGT]CC[CT]
2517 AGGTCCT
EcoRI 899 GAATTC G^AATTC
1011 GAATTC
EcoRV 2020 GATATC GAT^ATC
2354 GATATC
HaeII 42 GGCGCT [GA]GCGC^[CT]
992 GGCGCC
Hin1I 992 GGCGCC G[GA]^CG[CT]C
2764 GACGCC
Ksp632I 1261 CTCTTC CTCTTC(1/4)
2007 CTCTTC
NciI 305 CCCGG CC^[GC]GG
963 CCGGG
NcoI 905 CCATGG C^CATGG
1006 CCATGG
PpuMI 1841 AGGACCT [GA]G^G[AT]CC[CT]
2517 AGGTCCT
PstI 1722 CTGCAG CTGCA^G
2801 CTGCAG
RcaI 1694 TCATGA T^CATGA
2171 TCATGA
SmlI 144 CTCAAG C^T[CT][GA]AG
2256 CTCAAG
SspBI 1214 TGTACA T^GTACA
2162 TGTACA
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# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AvaII BalI
BanI BbvI BciVI BfaI BpmI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII