(Circular) (Six-base) MAPSORT of: pFN21AE3329.seq Check: 9318 from: 1 to: 7516 pFN21AE3329 7516 bp With 182 enzymes: SgfI * March 28, 2012 12:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5326 278 Size: 53 83 186 936 3790 2468 Fragments arranged by size: 3790 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4589 4859 1937 Size: 2652 270 4594 Fragments arranged by size: 4594 2652 270 Acc65I G'GTAC_C Cuts at: 4837 4837 Size: 7516 AclI AA'CG_TT Cuts at: 1563 5643 6016 1563 Size: 4080 373 3063 Fragments arranged by size: 4080 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4008 4167 4272 5579 6963 7231 1632 Size: 2376 159 105 1307 1384 268 1917 Fragments arranged by size: 2376 1917 1384 1307 268 159 105 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7319 AflIII A'CryG_T Cuts at: 1203 3337 4371 4665 6415 7392 1203 Size: 2134 1034 294 1750 977 1327 Fragments arranged by size: 2134 1750 1327 1034 977 294 AgeI A'CCGG_T Cuts at: 6347 6347 Size: 7516 AhdI GACnn_n'nnGTC Cuts at: 6245 6245 Size: 7516 AleI CACnn'nnGTG Cuts at: 1166 2957 1166 Size: 1791 5725 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7516 AlwNI CAG_nnn'CTG Cuts at: 2882 5259 6831 2882 Size: 2377 1572 3567 Fragments arranged by size: 3567 2377 1572 ApaLI G'TGCA_C Cuts at: 5573 6729 5573 Size: 1156 6360 ApoI r'AATT_y Cuts at: 1493 2904 3016 3560 3921 4007 4825 5051 5203 Size: 1411 112 544 361 86 818 226 152 Cuts at: 5203 7312 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1411 818 544 361 226 152 112 86 AseI AT'TA_AT Cuts at: 160 1032 3669 6070 160 Size: 872 2637 2401 1606 Fragments arranged by size: 2637 2401 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7516 AvaI C'yCGr_G Cuts at: 1870 1943 4378 4841 1870 Size: 73 2435 463 4545 Fragments arranged by size: 4545 2435 463 73 BamHI G'GATC_C Cuts at: 4628 4846 5213 4628 Size: 218 367 6931 Fragments arranged by size: 6931 367 218 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2997 3313 3623 4837 6292 Size: 325 217 627 1210 316 310 1214 1455 Cuts at: 6292 618 Size: 1842 Fragments arranged by size: 1842 1455 1214 1210 627 325 316 310 217 BanII G_rGCy'C Cuts at: 1391 2310 2876 4835 1391 Size: 919 566 1959 4072 Fragments arranged by size: 4072 1959 919 566 BbeI G_GCGC'C Cuts at: 3001 3001 Size: 7516 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4155 928 Size: 971 2256 4289 Fragments arranged by size: 4289 2256 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5707 5741 1903 Size: 34 50 34 3686 34 3678 Fragments arranged by size: 3686 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2474 2903 3036 4460 5408 6618 2474 Size: 429 133 1424 948 1210 3372 Fragments arranged by size: 3372 1424 1210 948 429 133 BclI T'GATC_A Cuts at: 1548 1548 Size: 7516 BfrBI ATG'CAT Cuts at: 3377 3377 Size: 7516 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2314 6127 136 Size: 107 122 71 631 605 642 3813 1525 Fragments arranged by size: 3813 1525 642 631 605 122 107 71 BglII A'GATC_T Cuts at: 2854 7511 2854 Size: 4657 2859 BlpI GC'TnA_GC Cuts at: 3504 4933 3504 Size: 1429 6087 Bme1580I G_kGCm'C Cuts at: 1790 5577 6733 1790 Size: 3787 1156 2573 Fragments arranged by size: 3787 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2211 3340 4234 1318 Size: 893 1129 894 4600 Fragments arranged by size: 4600 1129 894 893 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3016 4328 5247 Size: 416 53 83 399 24 1599 1312 919 Cuts at: 5247 6205 442 Size: 958 1753 Fragments arranged by size: 1753 1599 1312 958 919 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7516 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4276 4276 Size: 7516 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2454 2789 4058 6176 1239 Size: 1215 335 1269 2118 2579 Fragments arranged by size: 2579 2118 1269 1215 335 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2134 4246 5641 6506 6804 2134 Size: 2112 1395 865 298 2846 Fragments arranged by size: 2846 2112 1395 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4204 6179 882 Size: 617 2705 1975 2219 Fragments arranged by size: 2705 2219 1975 617 BsaAI yAC'GTr Cuts at: 493 1204 5307 493 Size: 711 4103 2702 Fragments arranged by size: 4103 2702 711 BsaBI GATnn'nnATC Cuts at: 7510 7510 Size: 7516 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2998 4770 5323 5705 Size: 53 83 186 936 1465 1772 553 382 Cuts at: 5705 6340 275 Size: 635 1451 Fragments arranged by size: 1772 1465 1451 936 635 553 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2895 5949 6347 6621 6768 7179 2895 Size: 3054 398 274 147 411 3232 Fragments arranged by size: 3232 3054 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4510 4540 1178 Size: 30 165 30 3107 30 4154 Fragments arranged by size: 4154 3107 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7516 BseYI C'CCAG_C Cuts at: 2862 3169 3546 6719 7215 2862 Size: 307 377 3173 496 3163 Fragments arranged by size: 3173 3163 496 377 307 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2219 3578 3767 1163 Size: 573 483 1359 189 4912 Fragments arranged by size: 4912 1359 573 483 189 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2015 4966 5727 6755 1540 Size: 275 186 14 2951 761 1028 2301 Fragments arranged by size: 2951 2301 1028 761 275 186 14 BsiHKAI G_wGCw'C Cuts at: 5577 5662 6733 5577 Size: 85 1071 6360 Fragments arranged by size: 6360 1071 85 BsmI GAATG_Cn' Cuts at: 3379 5027 5120 3379 Size: 1648 93 5775 Fragments arranged by size: 5775 1648 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2217 4761 2217 Size: 2544 4972 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2310 2876 4835 5577 5662 6733 1391 Size: 399 520 566 1959 742 85 1071 2174 Fragments arranged by size: 2174 1959 1071 742 566 520 399 85 BspEI T'CCGG_A Cuts at: 2895 7179 2895 Size: 4284 3232 BspHI T'CATG_A Cuts at: 3699 4176 5405 7135 3699 Size: 477 1229 1730 4080 Fragments arranged by size: 4080 1730 1229 477 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4871 844 Size: 4027 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2041 5403 1394 Size: 429 218 3362 3507 Fragments arranged by size: 3507 3362 429 218 BsrDI GCAATG_nn' Cuts at: 65 1248 3255 3557 3786 6011 6185 65 Size: 1183 2007 302 229 2225 174 1396 Fragments arranged by size: 2225 2007 1396 1183 302 229 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2010 6160 6347 1700 Size: 110 200 4150 187 2869 Fragments arranged by size: 4150 2869 200 187 110 BsrGI T'GTAC_A Cuts at: 96 3219 4167 96 Size: 3123 948 3445 Fragments arranged by size: 3445 3123 948 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7516 BssSI C'ACGA_G Cuts at: 5576 6588 5576 Size: 1012 6504 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7516 BstBI TT'CG_AA Cuts at: 6320 6320 Size: 7516 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7516 BstXI CCAn_nnnn'nTGG Cuts at: 2215 3250 4412 2215 Size: 1035 1162 5319 Fragments arranged by size: 5319 1162 1035 BstYI r'GATC_y Cuts at: 1972 2854 2917 3679 3704 4628 4846 5213 5598 Size: 882 63 762 25 924 218 367 385 Cuts at: 5598 5615 7067 7153 7165 7511 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 924 882 762 385 367 346 218 86 63 25 17 12 BtgI C'CryG_G Cuts at: 513 1065 2023 2910 3011 3769 513 Size: 552 958 887 101 758 4260 Fragments arranged by size: 4260 958 887 758 552 101 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2724 2766 487 Size: 683 56 1498 42 5237 Fragments arranged by size: 5237 1498 683 56 42 BtsI GCAGTG_nn' Cuts at: 2236 2466 3739 4543 4582 5032 5826 5846 2236 Size: 230 1273 804 39 450 794 20 3906 Fragments arranged by size: 3906 1273 804 794 450 230 39 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7516 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7481 DraI TTT'AAA Cuts at: 4222 4820 4222 Size: 598 6918 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4263 6523 809 Size: 507 2947 2260 1802 Fragments arranged by size: 2947 2260 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2012 2055 2070 Size: 54 1425 50 275 18 182 43 15 Cuts at: 2070 3240 3252 4600 4963 5852 7481 8 Size: 1170 12 1348 363 889 1629 43 Fragments arranged by size: 1629 1425 1348 1170 889 363 275 182 54 50 43 43 18 15 12 EagI C'GGCC_G Cuts at: 1537 1812 2012 4963 1537 Size: 275 200 2951 4090 Fragments arranged by size: 4090 2951 275 200 EarI CTCTTCn'nnn_ Cuts at: 1353 3272 4018 5446 1353 Size: 1919 746 1428 3423 Fragments arranged by size: 3423 1919 1428 746 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6103 6477 6623 387 Size: 1064 138 4514 374 146 1280 Fragments arranged by size: 4514 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2454 2789 4008 4058 4167 4272 5579 Size: 393 822 335 1219 50 109 105 1307 Cuts at: 5579 6176 6963 7231 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1307 1219 822 787 597 393 335 268 109 105 50 EcoO109I rG'GnC_Cy Cuts at: 3847 4523 3847 Size: 676 6840 EcoRI G'AATT_C Cuts at: 2904 3016 4825 5203 2904 Size: 112 1809 378 5217 Fragments arranged by size: 5217 1809 378 112 EcoRV GAT'ATC Cuts at: 4027 4361 4027 Size: 334 7182 FspI TGC'GCA Cuts at: 6022 6022 Size: 7516 HaeII r_GCGC'y Cuts at: 1678 2051 3001 6663 1678 Size: 373 950 3662 2531 Fragments arranged by size: 3662 2531 950 373 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4016 4048 6157 6189 6231 Size: 32 135 32 2444 32 2109 32 42 Cuts at: 6231 6263 1373 Size: 32 2626 Fragments arranged by size: 2626 2444 2109 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4590 4860 5106 669 Size: 988 281 2652 270 246 3079 Fragments arranged by size: 3079 2652 988 281 270 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7516 HpaI GTT'AAC Cuts at: 5106 5106 Size: 7516 KasI G'GCGC_C Cuts at: 2997 2997 Size: 7516 KpnI G_GTAC'C Cuts at: 4841 4841 Size: 7516 MfeI C'AATT_G Cuts at: 5115 5115 Size: 7516 MluI A'CGCG_T Cuts at: 7392 7392 Size: 7516 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3269 6630 6814 1305 Size: 1964 3361 184 2007 Fragments arranged by size: 3361 2007 1964 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3242 3254 4602 10 Size: 54 1425 343 1410 12 1348 2924 Fragments arranged by size: 2924 1425 1410 1348 343 54 12 MslI CAynn'nnrTG Cuts at: 518 1166 2957 3227 3717 4686 5474 5833 5992 Size: 648 1791 270 490 969 788 359 159 Cuts at: 5992 518 Size: 2042 Fragments arranged by size: 2042 1791 969 788 648 490 359 270 159 MspA1I CmG'CkG Cuts at: 1606 2025 2274 2615 3508 3806 3932 4502 4932 Size: 419 249 341 893 298 126 570 430 Cuts at: 4932 5609 6757 7002 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 893 677 570 430 419 341 298 249 245 126 NarI GG'CG_CC Cuts at: 2998 2998 Size: 7516 NcoI C'CATG_G Cuts at: 513 1065 2910 3011 513 Size: 552 1845 101 5018 Fragments arranged by size: 5018 1845 552 101 NdeI CA'TA_TG Cuts at: 387 387 Size: 7516 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7516 NotI GC'GGCC_GC Cuts at: 4963 4963 Size: 7516 NruI TCG'CGA Cuts at: 2751 2751 Size: 7516 NsiI A_TGCA'T Cuts at: 3379 3379 Size: 7516 NspI r_CATG'y Cuts at: 1132 1229 2538 2587 3655 4375 4669 4874 6419 Size: 97 1309 49 1068 720 294 205 1545 Cuts at: 6419 1132 Size: 2229 Fragments arranged by size: 2229 1545 1309 1068 720 294 205 97 49 PasI CC'CwG_GG Cuts at: 1348 3098 1348 Size: 1750 5766 PciI A'CATG_T Cuts at: 4371 4665 6415 4371 Size: 294 1750 5472 Fragments arranged by size: 5472 1750 294 PflMI CCAn_nnn'nTGG Cuts at: 1108 2274 2398 3975 4085 1108 Size: 1166 124 1577 110 4539 Fragments arranged by size: 4539 1577 1166 124 110 PfoI T'CCnGG_A Cuts at: 2301 2476 2766 4480 2301 Size: 175 290 1714 5337 Fragments arranged by size: 5337 1714 290 175 PmeI GTTT'AAAC Cuts at: 4820 4820 Size: 7516 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5585 5617 7098 7130 5585 Size: 32 1481 32 5971 Fragments arranged by size: 5971 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3847 4523 3847 Size: 676 6840 PshAI GACnn'nnGTC Cuts at: 1613 2745 1613 Size: 1132 6384 PsiI TTA'TAA Cuts at: 2946 5086 2946 Size: 2140 5376 PstI C_TGCA'G Cuts at: 830 3731 4810 4868 830 Size: 2901 1079 58 3478 Fragments arranged by size: 3478 2901 1079 58 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7516 PvuII CAG'CTG Cuts at: 2615 3508 3806 4502 2615 Size: 893 298 696 5629 Fragments arranged by size: 5629 893 696 298 SacII CC_GC'GG Cuts at: 2026 2026 Size: 7516 SalI G'TCGA_C Cuts at: 1936 4588 4858 1936 Size: 2652 270 4594 Fragments arranged by size: 4594 2652 270 SbfI CC_TGCA'GG Cuts at: 4868 4868 Size: 7516 ScaI AGT'ACT Cuts at: 5764 7476 5764 Size: 1712 5804 SfcI C'TryA_G Cuts at: 826 1045 3727 3940 4806 4864 5999 6680 6871 Size: 219 2682 213 866 58 1135 681 191 Cuts at: 6871 826 Size: 1471 Fragments arranged by size: 2682 1471 1135 866 681 219 213 191 58 SfoI GGC'GCC Cuts at: 2999 2999 Size: 7516 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7516 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7516 SmaI CCC'GGG Cuts at: 1872 4843 1872 Size: 2971 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2149 4261 5620 6521 6783 820 Size: 197 926 206 2112 1359 901 262 1553 Fragments arranged by size: 2112 1553 1359 926 901 262 206 197 SnaBI TAC'GTA Cuts at: 493 5307 493 Size: 4814 2702 SpeI A'CTAG_T Cuts at: 152 3226 152 Size: 3074 4442 SphI G_CATG'C Cuts at: 1132 3655 4874 1132 Size: 2523 1219 3774 Fragments arranged by size: 3774 2523 1219 SspI AAT'ATT Cuts at: 5 52 3607 3865 4648 4777 5440 5 Size: 47 3555 258 783 129 663 2081 Fragments arranged by size: 3555 2081 783 663 258 129 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7516 StyI C'CwwG_G Cuts at: 513 1065 2910 3011 3132 3192 4955 513 Size: 552 1845 101 121 60 1763 3074 Fragments arranged by size: 3074 1845 1763 552 121 101 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2316 3431 4174 4233 4554 5543 5560 Size: 1255 736 1115 743 59 321 989 17 Cuts at: 5560 5713 5898 6343 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1115 989 743 736 445 321 185 153 59 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2793 3219 4167 Size: 274 80 33 51 1443 816 426 948 Cuts at: 4167 5762 7474 96 Size: 1595 1712 138 Fragments arranged by size: 1712 1595 1443 948 816 426 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2458 2490 2521 2553 2965 2997 7476 7508 2458 Size: 32 31 32 412 32 4479 32 2466 Fragments arranged by size: 4479 2466 412 32 32 32 32 31 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7516 XbaI T'CTAG_A Cuts at: 4852 4852 Size: 7516 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4082 4082 Size: 7516 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7516 XmaI C'CCGG_G Cuts at: 1870 4841 1870 Size: 2971 4545 XmnI GAAnn'nnTTC Cuts at: 5645 5645 Size: 7516 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5324 276 Size: 53 83 186 936 3790 2468 Fragments arranged by size: 3790 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI NaeI NgoMIV PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI