(Linear) (Six-base) MAPSORT of: KIEE3329.seq Check: 5021 from: 1 to: 2811 KIEE3329.orf With 182 enzymes: SgfI * March 22, 2012 16:30 .. AccI GT'mk_AC Cuts at: 0 2584 2811 Size: 2584 227 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2003 2162 2267 2811 Size: 2003 159 105 544 Fragments arranged by size: 2003 544 159 105 AflIII A'CryG_T Cuts at: 0 1332 2366 2660 2811 Size: 1332 1034 294 151 Fragments arranged by size: 1332 1034 294 151 AleI CACnn'nnGTG Cuts at: 0 952 2811 Size: 952 1859 AlwNI CAG_nnn'CTG Cuts at: 0 877 2811 Size: 877 1934 ApoI r'AATT_y Cuts at: 0 899 1011 1555 1916 2002 2811 Size: 899 112 544 361 86 809 Fragments arranged by size: 899 809 544 361 112 86 AseI AT'TA_AT Cuts at: 0 1664 2811 Size: 1664 1147 AvaI C'yCGr_G Cuts at: 0 2373 2811 Size: 2373 438 BamHI G'GATC_C Cuts at: 0 2623 2811 Size: 2623 188 BanI G'GyrC_C Cuts at: 0 992 1308 1618 2811 Size: 992 316 310 1193 Fragments arranged by size: 1193 992 316 310 BanII G_rGCy'C Cuts at: 0 305 871 2811 Size: 305 566 1940 Fragments arranged by size: 1940 566 305 BbeI G_GCGC'C Cuts at: 0 996 2811 Size: 996 1815 BbsI GAAGACnn'nnnn_ Cuts at: 0 2150 2811 Size: 2150 661 BciVI GTATCCnnnnn_n' Cuts at: 0 469 898 1031 2455 2811 Size: 469 429 133 1424 356 Fragments arranged by size: 1424 469 429 356 133 BfrBI ATG'CAT Cuts at: 0 1372 2811 Size: 1372 1439 BglI GCCn_nnn'nGGC Cuts at: 0 309 2811 Size: 309 2502 BglII A'GATC_T Cuts at: 0 849 2811 Size: 849 1962 BlpI GC'TnA_GC Cuts at: 0 1499 2811 Size: 1499 1312 BmgBI CAC'GTC Cuts at: 0 206 1335 2229 2811 Size: 206 1129 894 582 Fragments arranged by size: 1129 894 582 206 BmrI ACTGGGnnnn_n' Cuts at: 0 1011 2323 2811 Size: 1011 1312 488 Fragments arranged by size: 1312 1011 488 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2271 2811 Size: 2271 540 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 449 784 2053 2811 Size: 449 335 1269 758 Fragments arranged by size: 1269 758 449 335 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 129 2241 2811 Size: 129 2112 570 Fragments arranged by size: 2112 570 129 BsaI GGTCTCn'nnnn_ Cuts at: 0 2199 2811 Size: 2199 612 BsaHI Gr'CG_yC Cuts at: 0 993 2765 2811 Size: 993 1772 46 Fragments arranged by size: 1772 993 46 BsaWI w'CCGG_w Cuts at: 0 890 2811 Size: 890 1921 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2505 2535 2811 Size: 2505 30 276 Fragments arranged by size: 2505 276 30 BseYI C'CCAG_C Cuts at: 0 857 1164 1541 2811 Size: 857 307 377 1270 Fragments arranged by size: 1270 857 377 307 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 214 1573 1762 2811 Size: 214 1359 189 1049 Fragments arranged by size: 1359 1049 214 189 BsiEI CG_ry'CG Cuts at: 0 10 2811 Size: 10 2801 BsmI GAATG_Cn' Cuts at: 0 1374 2811 Size: 1374 1437 BsmBI CGTCTCn'nnnn_ Cuts at: 0 212 2756 2811 Size: 212 2544 55 Fragments arranged by size: 2544 212 55 Bsp1286I G_dGCh'C Cuts at: 0 305 871 2811 Size: 305 566 1940 Fragments arranged by size: 1940 566 305 BspEI T'CCGG_A Cuts at: 0 890 2811 Size: 890 1921 BspHI T'CATG_A Cuts at: 0 1694 2171 2811 Size: 1694 477 640 Fragments arranged by size: 1694 640 477 BsrBI CCG'CTC Cuts at: 0 36 2811 Size: 36 2775 BsrDI GCAATG_nn' Cuts at: 0 1250 1552 1781 2811 Size: 1250 302 229 1030 Fragments arranged by size: 1250 1030 302 229 BsrFI r'CCGG_y Cuts at: 0 5 2811 Size: 5 2806 BsrGI T'GTAC_A Cuts at: 0 1214 2162 2811 Size: 1214 948 649 Fragments arranged by size: 1214 948 649 BstXI CCAn_nnnn'nTGG Cuts at: 0 210 1245 2407 2811 Size: 210 1035 1162 404 Fragments arranged by size: 1162 1035 404 210 BstYI r'GATC_y Cuts at: 0 849 912 1674 1699 2623 2811 Size: 849 63 762 25 924 188 Fragments arranged by size: 924 849 762 188 63 25 BtgI C'CryG_G Cuts at: 0 18 905 1006 1764 2811 Size: 18 887 101 758 1047 Fragments arranged by size: 1047 887 758 101 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 719 761 2811 Size: 719 42 2050 Fragments arranged by size: 2050 719 42 BtsI GCAGTG_nn' Cuts at: 0 231 461 1734 2538 2577 2811 Size: 231 230 1273 804 39 234 Fragments arranged by size: 1273 804 234 231 230 39 DraI TTT'AAA Cuts at: 0 2217 2811 Size: 2217 594 DrdI GACnn_nn'nnGTC Cuts at: 0 2258 2811 Size: 2258 553 EaeI y'GGCC_r Cuts at: 0 7 50 65 1235 1247 2595 2811 Size: 7 43 15 1170 12 1348 216 Fragments arranged by size: 1348 1170 216 43 15 12 7 EagI C'GGCC_G Cuts at: 0 7 2811 Size: 7 2804 EarI CTCTTCn'nnn_ Cuts at: 0 1267 2013 2811 Size: 1267 746 798 Fragments arranged by size: 1267 798 746 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 449 784 2003 2053 2162 2267 2811 Size: 449 335 1219 50 109 105 544 Fragments arranged by size: 1219 544 449 335 109 105 50 EcoO109I rG'GnC_Cy Cuts at: 0 1842 2518 2811 Size: 1842 676 293 Fragments arranged by size: 1842 676 293 EcoRI G'AATT_C Cuts at: 0 899 1011 2811 Size: 899 112 1800 Fragments arranged by size: 1800 899 112 EcoRV GAT'ATC Cuts at: 0 2022 2356 2811 Size: 2022 334 455 Fragments arranged by size: 2022 455 334 HaeII r_GCGC'y Cuts at: 0 46 996 2811 Size: 46 950 1815 Fragments arranged by size: 1815 950 46 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 2011 2043 2811 Size: 2011 32 768 Fragments arranged by size: 2011 768 32 HincII GTy'rAC Cuts at: 0 2585 2811 Size: 2585 226 KasI G'GCGC_C Cuts at: 0 992 2811 Size: 992 1819 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1264 2811 Size: 1264 1547 MscI TGG'CCA Cuts at: 0 1237 1249 2597 2811 Size: 1237 12 1348 214 Fragments arranged by size: 1348 1237 214 12 MslI CAynn'nnrTG Cuts at: 0 952 1222 1712 2681 2811 Size: 952 270 490 969 130 Fragments arranged by size: 969 952 490 270 130 MspA1I CmG'CkG Cuts at: 0 20 269 610 1503 1801 1927 2497 2811 Size: 20 249 341 893 298 126 570 314 Fragments arranged by size: 893 570 341 314 298 249 126 20 NarI GG'CG_CC Cuts at: 0 993 2811 Size: 993 1818 NcoI C'CATG_G Cuts at: 0 905 1006 2811 Size: 905 101 1805 Fragments arranged by size: 1805 905 101 NruI TCG'CGA Cuts at: 0 746 2811 Size: 746 2065 NsiI A_TGCA'T Cuts at: 0 1374 2811 Size: 1374 1437 NspI r_CATG'y Cuts at: 0 533 582 1650 2370 2664 2811 Size: 533 49 1068 720 294 147 Fragments arranged by size: 1068 720 533 294 147 49 PasI CC'CwG_GG Cuts at: 0 1093 2811 Size: 1093 1718 PciI A'CATG_T Cuts at: 0 2366 2660 2811 Size: 2366 294 151 Fragments arranged by size: 2366 294 151 PflMI CCAn_nnn'nTGG Cuts at: 0 269 393 1970 2080 2811 Size: 269 124 1577 110 731 Fragments arranged by size: 1577 731 269 124 110 PfoI T'CCnGG_A Cuts at: 0 296 471 761 2475 2811 Size: 296 175 290 1714 336 Fragments arranged by size: 1714 336 296 290 175 PpuMI rG'GwC_Cy Cuts at: 0 1842 2518 2811 Size: 1842 676 293 Fragments arranged by size: 1842 676 293 PshAI GACnn'nnGTC Cuts at: 0 740 2811 Size: 740 2071 PsiI TTA'TAA Cuts at: 0 941 2811 Size: 941 1870 PstI C_TGCA'G Cuts at: 0 1726 2805 2811 Size: 1726 1079 6 Fragments arranged by size: 1726 1079 6 PvuII CAG'CTG Cuts at: 0 610 1503 1801 2497 2811 Size: 610 893 298 696 314 Fragments arranged by size: 893 696 610 314 298 SacII CC_GC'GG Cuts at: 0 21 2811 Size: 21 2790 SalI G'TCGA_C Cuts at: 0 2583 2811 Size: 2583 228 SfcI C'TryA_G Cuts at: 0 1722 1935 2801 2811 Size: 1722 213 866 10 Fragments arranged by size: 1722 866 213 10 SfoI GGC'GCC Cuts at: 0 994 2811 Size: 994 1817 SmlI C'TyrA_G Cuts at: 0 144 2256 2811 Size: 144 2112 555 Fragments arranged by size: 2112 555 144 SpeI A'CTAG_T Cuts at: 0 1221 2811 Size: 1221 1590 SphI G_CATG'C Cuts at: 0 1650 2811 Size: 1650 1161 SspI AAT'ATT Cuts at: 0 1602 1860 2643 2772 2811 Size: 1602 258 783 129 39 Fragments arranged by size: 1602 783 258 129 39 StyI C'CwwG_G Cuts at: 0 905 1006 1127 1187 2811 Size: 905 101 121 60 1624 Fragments arranged by size: 1624 905 121 101 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 311 1426 2169 2228 2549 2811 Size: 311 1115 743 59 321 262 Fragments arranged by size: 1115 743 321 311 262 59 TatI w'GTAC_w Cuts at: 0 788 1214 2162 2811 Size: 788 426 948 649 Fragments arranged by size: 948 788 649 426 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 453 485 516 548 960 992 2811 Size: 453 32 31 32 412 32 1819 Fragments arranged by size: 1819 453 412 32 32 32 31 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2077 2811 Size: 2077 734 Enzymes that do cut: AccI AcuI AflIII AleI AlwNI ApoI AseI AvaI BamHI BanI BanII BbeI BbsI BciVI BfrBI BglI BglII BlpI BmgBI BmrI BplI BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BsrGI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI Eco57MI EcoO109I EcoRI EcoRV HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NarI NcoI NruI NsiI NspI PasI PciI PflMI PfoI PpuMI PshAI PsiI PstI PvuII SacII SalI SfcI SfoI SmlI SpeI SphI SspI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI ApaI ApaLI AscI AsiSI AvrII BaeI BbvCI BcgI BclI Bme1580I BmtI Bpu10I BsaAI BsaBI BseRI BsiHKAI BsiWI BspMI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EciI EcoICRI EcoNI FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI PacI PmeI PmlI PpiI PspOMI PspXI PsrI PvuI RsrII SacI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SrfI StuI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI