################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvaI MvnI MwoI NaeI NarI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI StuI SwaI XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 814 GTCTAC GT^[AC][GT]AC AfaI 737 GTAC GT^AC Asp700I 388 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 662 GAGCAC G[AT]GC[AT]^C BbvI 258 GCTGC GCAGC(8/12) BcnI 1119 CCCGG CC^[GC]GG BmyI 662 GAGCAC G[AGT]GC[ACT]^C BseRI 1281 GAGGAG GAGGAG(10/8) BsiHKAI 662 GAGCAC G[AT]GC[AT]^C BsmAI 1305 GTCTC GTCTC(1/5) BsmI 722 GCATTC GAATGC(1/-1) Bsp1286I 662 GAGCAC G[AGT]GC[ACT]^C BsrDI 1100 GCAATG GCAATG(2/0) BssKI 1119 CCCGG ^CC[ACGT]GG BstF5I 1354 CATCC GGATG(2/0) EcoT14I 841 CCATGG C^C[AT][AT]GG Fnu4HI 258 GCTGC GC^[ACGT]GC FokI 1354 CATCC GGATG(9/13) HapII 1120 CCGG C^CGG HindIII 1371 AAGCTT A^AGCTT HpaII 1120 CCGG C^CGG HphI 757 GGTGA GGTGA(8/7) ItaI 258 GCTGC GC^[ACGT]GC MaeII 892 ACGT A^CGT MslI 763 CATACAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 1120 CCGG C^CGG NciI 1119 CCCGG CC^[GC]GG NcoI 841 CCATGG C^CATGG NspI 773 GCATGT [GA]CATG^[CT] PflMI 537 CCAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 737 GTAC GT^AC SapI 424 GAAGAGC GCTCTTC(1/4) ScrFI 1119 CCCGG CC^[ACGT]GG SfaNI 521 GCATC GCATC(5/9) SmlI 800 CTCAAG C^T[CT][GA]AG SpeI 616 ACTAGT A^CTAGT StyI 841 CCATGG C^C[AT][AT]GG TaqI 861 TCGA T^CGA TseI 258 GCTGC G^C[AT]GC Tsp45I 348 GTCAC ^GT[GC]AC TspRI 267 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 861 TCGA T^CGA Van91I 537 CCAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 388 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AseI 202 ATTAAT AT^TAAT 849 ATTAAT AsnI 202 ATTAAT AT^TAAT 849 ATTAAT AspI 161 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC 175 GACTTTGTC BbsI 547 GAAGAC GAAGAC(2/6) 1006 GAAGAC BclI 57 TGATCA T^GATCA 234 TGATCA BpuAI 547 GAAGAC GAAGAC(2/6) 1006 GAAGAC BsaJI 542 CCGTGG C^C[ACGT][ACGT]GG 841 CCATGG BsiYI 150 CCTTCAGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 537 CCAGACCGTGG BslI 150 CCTTCAGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 537 CCAGACCGTGG BsmFI 159 GGGAC GGGAC(10/14) 1105 GTCCC Cfr13I 195 GGGCC G^G[ACGT]CC 945 GGGCC DsaI 542 CCGTGG C^C[GA][CT]GG 841 CCATGG FbaI 57 TGATCA T^GATCA 234 TGATCA HinfI 79 GATTC G^A[ACGT]TC 1363 GATTC MaeIII 348 GTCAC ^GT[ACGT]AC 481 GTAAC PflFI 161 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC 175 GACTTTGTC PshBI 202 ATTAAT AT^TAAT 849 ATTAAT Sau96I 195 GGGCC G^G[ACGT]CC 945 GGGCC SspI 416 AATATT AAT^ATT 1037 AATATT TfiI 79 GATTC G^A[AT]TC 1363 GATTC Tth111I 161 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC 175 GACTTTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AcsI AluI AlwI ApoI BfaI BpmI BsrI DdeI DpnII EarI HaeIII Ksp632I MaeI MboI MboII MnlI MseI NdeII NlaIII Sau3AI Tru9I Tsp509I