(Circular) (Six-base) MAPSORT of: pFN21AE1923.seq Check: 803 from: 1 to: 6085 pFN21AE1923 6085 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3895 278 Size: 53 83 186 936 2359 2468 Fragments arranged by size: 2468 2359 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2820 3428 1937 Size: 883 608 4594 Fragments arranged by size: 4594 883 608 Acc65I G'GTAC_C Cuts at: 3406 3406 Size: 6085 AclI AA'CG_TT Cuts at: 1563 4212 4585 1563 Size: 2649 373 3063 Fragments arranged by size: 3063 2649 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2141 2675 4148 5532 5800 1632 Size: 509 534 1473 1384 268 1917 Fragments arranged by size: 1917 1473 1384 534 509 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5888 AflIII A'CryG_T Cuts at: 1203 4984 5961 1203 Size: 3781 977 1327 Fragments arranged by size: 3781 1327 977 AgeI A'CCGG_T Cuts at: 4916 4916 Size: 6085 AhdI GACnn_n'nnGTC Cuts at: 4814 4814 Size: 6085 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6085 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6085 AlwNI CAG_nnn'CTG Cuts at: 3828 5400 3828 Size: 1572 4513 ApaLI G'TGCA_C Cuts at: 4142 5298 4142 Size: 1156 4929 ApoI r'AATT_y Cuts at: 1493 2136 2397 2643 2939 3394 3620 3772 5881 Size: 643 261 246 296 455 226 152 2109 Cuts at: 5881 1493 Size: 1697 Fragments arranged by size: 2109 1697 643 455 296 261 246 226 152 AseI AT'TA_AT Cuts at: 160 1032 2208 2855 4639 160 Size: 872 1176 647 1784 1606 Fragments arranged by size: 1784 1606 1176 872 647 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6085 AvaI C'yCGr_G Cuts at: 1870 1943 3410 1870 Size: 73 1467 4545 Fragments arranged by size: 4545 1467 73 BamHI G'GATC_C Cuts at: 3415 3782 3415 Size: 367 5718 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3406 4861 618 Size: 325 217 627 1619 1455 1842 Fragments arranged by size: 1842 1619 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3404 1391 Size: 2013 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2559 3018 928 Size: 971 660 459 3995 Fragments arranged by size: 3995 971 660 459 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4276 4310 1903 Size: 34 2339 34 3678 Fragments arranged by size: 3678 2339 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3977 5187 3977 Size: 1210 4875 BclI T'GATC_A Cuts at: 1548 2062 2239 1548 Size: 514 177 5394 Fragments arranged by size: 5394 514 177 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4696 136 Size: 107 122 71 631 605 3024 1525 Fragments arranged by size: 3024 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6080 6080 Size: 6085 BlpI GC'TnA_GC Cuts at: 3502 3502 Size: 6085 Bme1580I G_kGCm'C Cuts at: 1790 4146 5302 1790 Size: 2356 1156 2573 Fragments arranged by size: 2573 2356 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6085 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3816 4774 442 Size: 416 53 83 399 24 2399 958 1753 Fragments arranged by size: 2399 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6085 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2038 2079 2454 2525 4745 1239 Size: 799 41 375 71 2220 2579 Fragments arranged by size: 2579 2220 799 375 71 41 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2790 4210 5075 5373 2790 Size: 1420 865 298 3502 Fragments arranged by size: 3502 1420 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4748 882 Size: 617 3249 2219 Fragments arranged by size: 3249 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3876 493 Size: 711 2672 2702 Fragments arranged by size: 2702 2672 711 BsaBI GATnn'nnATC Cuts at: 6079 6079 Size: 6085 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3892 4274 4909 275 Size: 53 83 186 936 2359 382 635 1451 Fragments arranged by size: 2359 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4518 4916 5190 5337 5748 4518 Size: 398 274 147 411 4855 Fragments arranged by size: 4855 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5860 Fragments arranged by size: 5860 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3301 1186 Size: 2115 3970 BseYI C'CCAG_C Cuts at: 2795 5288 5784 2795 Size: 2493 496 3096 Fragments arranged by size: 3096 2493 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5512 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3535 4296 5324 1540 Size: 275 186 1534 761 1028 2301 Fragments arranged by size: 2301 1534 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2671 4146 4231 5302 2671 Size: 1475 85 1071 3454 Fragments arranged by size: 3454 1475 1071 85 BsmI GAATG_Cn' Cuts at: 2727 3596 3689 2727 Size: 869 93 5123 Fragments arranged by size: 5123 869 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2671 3404 4146 4231 5302 1391 Size: 399 881 733 742 85 1071 2174 Fragments arranged by size: 2174 1071 881 742 733 399 85 BspEI T'CCGG_A Cuts at: 5748 5748 Size: 6085 BspHI T'CATG_A Cuts at: 3974 5704 3974 Size: 1730 4355 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3440 844 Size: 2596 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3972 1394 Size: 429 2149 3507 Fragments arranged by size: 3507 2149 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3112 4580 4754 65 Size: 1183 1864 1468 174 1396 Fragments arranged by size: 1864 1468 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4729 4916 1700 Size: 110 2919 187 2869 Fragments arranged by size: 2919 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6085 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6085 BssSI C'ACGA_G Cuts at: 4145 5157 4145 Size: 1012 5073 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6085 BstBI TT'CG_AA Cuts at: 4889 4889 Size: 6085 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6085 BstYI r'GATC_y Cuts at: 1972 3415 3782 4167 4184 5636 5722 5734 6080 Size: 1443 367 385 17 1452 86 12 346 Cuts at: 6080 1972 Size: 1977 Fragments arranged by size: 1977 1452 1443 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2547 2846 513 Size: 552 1482 299 3752 Fragments arranged by size: 3752 1482 552 299 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5346 Fragments arranged by size: 5346 683 56 BtsI GCAGTG_nn' Cuts at: 3601 4395 4415 3601 Size: 794 20 5271 Fragments arranged by size: 5271 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6085 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6050 DraI TTT'AAA Cuts at: 3389 3389 Size: 6085 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5092 809 Size: 507 3776 1802 Fragments arranged by size: 3776 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3532 4421 6050 Size: 54 1425 50 275 18 1702 889 1629 Cuts at: 6050 8 Size: 43 Fragments arranged by size: 1702 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3532 1537 Size: 275 1720 4090 Fragments arranged by size: 4090 1720 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2331 2424 3286 4015 1353 Size: 978 93 862 729 3423 Fragments arranged by size: 3423 978 862 729 93 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4672 5046 5192 387 Size: 1064 138 3083 374 146 1280 Fragments arranged by size: 3083 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2038 2079 2141 2454 2525 2675 4148 Size: 393 406 41 62 313 71 150 1473 Cuts at: 4148 4745 5532 5800 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1473 787 597 406 393 313 268 150 71 62 41 EcoRI G'AATT_C Cuts at: 3394 3772 3394 Size: 378 5707 FspI TGC'GCA Cuts at: 4591 4591 Size: 6085 HaeII r_GCGC'y Cuts at: 1678 5232 1678 Size: 3554 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2060 2075 2090 2092 2107 Size: 32 135 32 488 15 15 2 15 Cuts at: 2107 2122 2819 2851 4726 4758 4800 4832 1373 Size: 15 697 32 1875 32 42 32 2626 Fragments arranged by size: 2626 1875 697 488 135 42 32 32 32 32 32 15 15 15 15 2 HincII GTy'rAC Cuts at: 669 1657 1938 3429 3675 669 Size: 988 281 1491 246 3079 Fragments arranged by size: 3079 1491 988 281 246 HindIII A'AGCT_T Cuts at: 748 3376 748 Size: 2628 3457 HpaI GTT'AAC Cuts at: 3675 3675 Size: 6085 KpnI G_GTAC'C Cuts at: 3410 3410 Size: 6085 MfeI C'AATT_G Cuts at: 3684 3684 Size: 6085 MluI A'CGCG_T Cuts at: 5961 5961 Size: 6085 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2988 3386 5199 5383 1305 Size: 1683 398 1813 184 2007 Fragments arranged by size: 2007 1813 1683 398 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4263 Fragments arranged by size: 4263 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2772 4043 4402 4561 518 Size: 648 1606 1271 359 159 2042 Fragments arranged by size: 2042 1606 1271 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3501 4178 5326 5571 1606 Size: 1895 677 1148 245 2120 Fragments arranged by size: 2120 1895 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2846 513 Size: 552 1781 3752 Fragments arranged by size: 3752 1781 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6085 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6085 NotI GC'GGCC_GC Cuts at: 3532 3532 Size: 6085 NspI r_CATG'y Cuts at: 1132 1229 2782 3443 4988 1132 Size: 97 1553 661 1545 2229 Fragments arranged by size: 2229 1553 1545 661 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6085 PciI A'CATG_T Cuts at: 4984 4984 Size: 6085 PflMI CCAn_nnn'nTGG Cuts at: 1108 2548 1108 Size: 1440 4645 PfoI T'CCnGG_A Cuts at: 3123 3123 Size: 6085 PmeI GTTT'AAAC Cuts at: 3389 3389 Size: 6085 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4154 4186 5667 5699 4154 Size: 32 1481 32 4540 Fragments arranged by size: 4540 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6085 PsiI TTA'TAA Cuts at: 2754 3655 2754 Size: 901 5184 PstI C_TGCA'G Cuts at: 830 3437 830 Size: 2607 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6085 SalI G'TCGA_C Cuts at: 1936 3427 1936 Size: 1491 4594 SapI GCTCTTCn'nnn_ Cuts at: 2424 2424 Size: 6085 SbfI CC_TGCA'GG Cuts at: 3437 3437 Size: 6085 ScaI AGT'ACT Cuts at: 4333 6045 4333 Size: 1712 4373 SfcI C'TryA_G Cuts at: 826 1045 3433 4568 5249 5440 826 Size: 219 2388 1135 681 191 1471 Fragments arranged by size: 2388 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6085 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6085 SmaI CCC'GGG Cuts at: 1872 3412 1872 Size: 1540 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2805 4189 5090 5352 820 Size: 197 926 862 1384 901 262 1553 Fragments arranged by size: 1553 1384 926 901 862 262 197 SnaBI TAC'GTA Cuts at: 493 3876 493 Size: 3383 2702 SpeI A'CTAG_T Cuts at: 152 2621 152 Size: 2469 3616 SphI G_CATG'C Cuts at: 1132 3443 1132 Size: 2311 3774 SspI AAT'ATT Cuts at: 5 52 2423 3044 4009 5 Size: 47 2371 621 965 2081 Fragments arranged by size: 2371 2081 965 621 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6085 StyI C'CwwG_G Cuts at: 513 1065 2846 3524 513 Size: 552 1781 678 3074 Fragments arranged by size: 3074 1781 678 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2493 4112 4129 4282 4467 4912 325 Size: 1255 913 1619 17 153 185 445 1498 Fragments arranged by size: 1619 1498 1255 913 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4331 6043 96 Size: 274 80 33 51 1443 2354 1712 138 Fragments arranged by size: 2354 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6045 6077 6045 Size: 32 6053 Tth111I GACn'n_nGTC Cuts at: 1316 2169 2183 1316 Size: 853 14 5218 Fragments arranged by size: 5218 853 14 XbaI T'CTAG_A Cuts at: 3421 3421 Size: 6085 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6085 XmaI C'CCGG_G Cuts at: 1870 3410 1870 Size: 1540 4545 XmnI GAAnn'nnTTC Cuts at: 2397 4214 2397 Size: 1817 4268 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3893 276 Size: 53 83 186 936 2359 2468 Fragments arranged by size: 2468 2359 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI