################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AccI AccII AccIII AclI AflII AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspHI AspI AviII AvrII BamHI BbeI BcgI BciVI BclI BcnI BfrI BlnI BseAI BseRI BsiHKAI BsiWI BsmBI BsmI BspDI BspEI BspMI BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstF5I BstUI BstZ17I CfoI ClaI CpoI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoRI EcoRV FbaI FokI FseI FspI HaeII HhaI HinP1I HindIII KasI Ksp632I KspI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PmeI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 855 GACGTC GACGT^C Acc65I 960 GGTACC G^GTACC AcsI 230 AAATTC [GA]^AATT[CT] AgeI 1184 ACCGGT A^CCGGT AlwNI 612 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1487 CCTCAGG CC^T[ACGT]AGG ApoI 230 AAATTC [GA]^AATT[CT] Asp700I 881 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 960 GGTACC G^GTACC AvaI 1554 CCCGAG C^[CT]CG[GA]G BbrPI 255 CACGTG CAC^GTG BbvCI 1371 CCTCAGC CCTCAGC(-5/-2) BfaI 796 CTAG C^TAG BglI 1321 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1200 AGATCT A^GATCT BlpI 1126 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1126 GCTCAGC GC^T[ACGT]AGC BsaAI 255 CACGTG [CT]AC^GT[GA] BsaBI 316 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 68 GTGCAG GTGCAG(16/14) BsiEI 758 CGGTCG CG[GA][CT]^CG BsoBI 1554 CCCGAG C^[CT]CG[GA]G BspHI 1640 TCATGA T^CATGA BspLU11I 1158 ACATGT A^CATGT BsrFI 1184 ACCGGT [GA]^CCGG[CT] BstEII 1221 GGTCACC G^GT[ACGT]ACC BstPI 1221 GGTCACC G^GT[ACGT]ACC Bsu36I 1487 CCTCAGG CC^T[ACGT]AGG CelII 1126 GCTCAGC GC^T[ACGT]AGC Cfr10I 1184 ACCGGT [GA]^CCGG[CT] DraI 222 TTTAAA TTT^AAA Eco81I 1487 CCTCAGG CC^T[ACGT]AGG EcoO65I 1221 GGTCACC G^GT[ACGT]ACC FauI 493 GCGGG CCCGC(4/6) HgaI 932 GCGTC GACGC(5/10) HpaI 367 GTTAAC GTT^AAC KpnI 960 GGTACC GGTAC^C MaeI 796 CTAG C^TAG MamI 316 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 1419 CATGCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PinAI 1184 ACCGGT A^CCGGT PmaCI 255 CACGTG CAC^GTG PmlI 255 CACGTG CAC^GTG RcaI 1640 TCATGA T^CATGA ScaI 1348 AGTACT AGT^ACT SfcI 1561 CTACAG C^T[GA][CT]AG SmlI 468 CTTGAG C^T[CT][GA]AG StuI 1368 AGGCCT AGG^CCT XmnI 881 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AcyI 855 GACGTC G[GA]^CG[CT]C 931 GGCGTC AflIII 256 ACGTGT A^C[GA][CT]GT 1158 ACATGT BalI 1165 TGGCCA TGG^CCA 1274 TGGCCA BanI 960 GGTACC G^G[CT][GA]CC 1477 GGTGCC BanII 133 GAGCCC G[GA]GC[CT]^C 709 GGGCTC BbsI 637 GAAGAC GAAGAC(2/6) 885 GTCTTC BpmI 400 CTCCAG CTGGAG(16/14) 689 CTGGAG BpuAI 637 GAAGAC GAAGAC(2/6) 885 GTCTTC BsaHI 855 GACGTC G[GA]^CG[CT]C 931 GGCGTC BsaWI 1184 ACCGGT [AT]^CCGG[AT] 1225 ACCGGA Bsp1407I 207 TGTACA T^GTACA 1506 TGTACA BsrDI 1290 CATTGC GCAATG(2/0) 1516 GCAATG BsrGI 207 TGTACA T^GTACA 1506 TGTACA BstXI 928 CCAGGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1289 CCATTGCTTTGG BstYI 1110 GGATCT [GA]^GATC[CT] 1200 AGATCT EaeI 1165 TGGCCA [CT]^GGCC[GA] 1274 TGGCCA EcoT22I 1088 ATGCAT ATGCA^T 1399 ATGCAT HapII 1185 CCGG C^CGG 1226 CCGG Hin1I 855 GACGTC G[GA]^CG[CT]C 931 GGCGTC HincII 301 GTCAAC GT[CT]^[GA]AC 367 GTTAAC HindII 301 GTCAAC GT[CT]^[GA]AC 367 GTTAAC HpaII 1185 CCGG C^CGG 1226 CCGG MboII 637 GAAGA GAAGA(8/7) 886 TCTTC MflI 1110 GGATCT [GA]^GATC[CT] 1200 AGATCT MluNI 1165 TGGCCA TGG^CCA 1274 TGGCCA MscI 1165 TGGCCA TGG^CCA 1274 TGGCCA MspI 1185 CCGG C^CGG 1226 CCGG MwoI 757 GCGGTCGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1321 GCCATGGAGGC NsiI 1088 ATGCAT ATGCA^T 1399 ATGCAT PleI 1302 GACTC GAGTC(4/5) 1447 GAGTC PpuMI 92 GGGTCCT [GA]G^G[AT]CC[CT] 1244 AGGACCT SphI 1401 GCATGC GCATG^C 1418 GCATGC SspBI 207 TGTACA T^GTACA 1506 TGTACA TfiI 316 GATTC G^A[AT]TC 1380 GAATC XhoII 1110 GGATCT [GA]^GATC[CT] 1200 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BbvI BmyI BsaI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII EcoT14I Fnu4HI HaeIII HinfI HphI ItaI MaeII MaeIII MboI MnlI MseI MvaI NcoI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI