Restriction map for
pFN21AE3249
(Circular) (Six-base) MAPSORT of: pFN21AE3249.seq Check: 9341 from: 1 to: 6358
pFN21AE3249 6358 bp
With 182 enzymes: SgfI *
March 28, 2012 12:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2864 4168 278
Size: 53 83 186 936 1328 1304 2468
Fragments arranged by size:
2468 1328 1304 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3701 1937
Size: 1764 4594
Acc65I G'GTAC_C
Cuts at: 2965 3679 2965
Size: 714 5644
AclI AA'CG_TT
Cuts at: 1563 4485 4858 1563
Size: 2922 373 3063
Fragments arranged by size:
3063 2922 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3629 4421 5805 6073 1632
Size: 1997 792 1384 268 1917
Fragments arranged by size:
1997 1917 1384 792 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6161
AflIII A'CryG_T
Cuts at: 1203 2261 3163 5257 6234 1203
Size: 1058 902 2094 977 1327
Fragments arranged by size:
2094 1327 1058 977 902
AgeI A'CCGG_T
Cuts at: 3189 5189 3189
Size: 2000 4358
AhdI GACnn_n'nnGTC
Cuts at: 5087 5087
Size: 6358
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6358
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3423 1184
Size: 2239 4119
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2129 2161 2129
Size: 32 6326
AlwNI CAG_nnn'CTG
Cuts at: 2622 4101 5673 2622
Size: 1479 1572 3307
Fragments arranged by size:
3307 1572 1479
ApaLI G'TGCA_C
Cuts at: 4415 5571 4415
Size: 1156 5202
ApoI r'AATT_y
Cuts at: 1493 2235 3667 3893 4045 6154 1493
Size: 742 1432 226 152 2109 1697
Fragments arranged by size:
2109 1697 1432 742 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4912 160
Size: 872 3880 1606
Fragments arranged by size:
3880 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6358
AvaI C'yCGr_G
Cuts at: 1870 1943 3559 3683 1870
Size: 73 1616 124 4545
Fragments arranged by size:
4545 1616 124 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2957 2990 2957
Size: 33 6325
BamHI G'GATC_C
Cuts at: 3688 4055 3688
Size: 367 5991
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2965 3482 3679 5134 618
Size: 325 217 627 1178 517 197 1455 1842
Fragments arranged by size:
1842 1455 1178 627 517 325 217 197
BanII G_rGCy'C
Cuts at: 1391 2142 2718 3677 1391
Size: 751 576 959 4072
Fragments arranged by size:
4072 959 751 576
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2649 2883 928
Size: 971 750 234 4403
Fragments arranged by size:
4403 971 750 234
BbvCI CC'TCA_GC
Cuts at: 3377 3377
Size: 6358
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4549 4583 1903
Size: 34 2612 34 3678
Fragments arranged by size:
3678 2612 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4250 5460 4250
Size: 1210 5148
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6358
BfrBI ATG'CAT
Cuts at: 3095 3406 3095
Size: 311 6047
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3332 4969 136
Size: 107 122 71 631 605 1660 1637 1525
Fragments arranged by size:
1660 1637 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3205 6353 3205
Size: 3148 3210
BlpI GC'TnA_GC
Cuts at: 3132 3775 3132
Size: 643 5715
Bme1580I G_kGCm'C
Cuts at: 1790 2832 3345 4419 5575 1790
Size: 1042 513 1074 1156 2573
Fragments arranged by size:
2573 1156 1074 1042 513
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6358
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2137 4089 5047
Size: 416 53 83 399 24 720 1952 958
Cuts at: 5047 442
Size: 1753
Fragments arranged by size:
1952 1753 958 720 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6358
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2182 2182
Size: 6358
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2390 2715 5018 1239
Size: 1151 325 2303 2579
Fragments arranged by size:
2579 2303 1151 325
Bpu10I CC'TnA_GC
Cuts at: 3199 3377 3199
Size: 178 6180
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2494 4483 5348 5646 2494
Size: 1989 865 298 3206
Fragments arranged by size:
3206 1989 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2381 2565 2730 3198 5021 882
Size: 617 882 184 165 468 1823 2219
Fragments arranged by size:
2219 1823 882 617 468 184 165
BsaAI yAC'GTr
Cuts at: 493 1204 2262 4149 493
Size: 711 1058 1887 2702
Fragments arranged by size:
2702 1887 1058 711
BsaBI GATnn'nnATC
Cuts at: 2325 6352 2325
Size: 4027 2331
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2861 2937 4165 4547
Size: 53 83 186 936 1328 76 1228 382
Cuts at: 4547 5182 275
Size: 635 1451
Fragments arranged by size:
1451 1328 1228 936 635 382 186 83
76 53
BsaWI w'CCGG_w
Cuts at: 3189 3230 4791 5189 5463 5610 6021 3189
Size: 41 1561 398 274 147 411 3526
Fragments arranged by size:
3526 1561 411 398 274 147 41
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2129 2159 1178
Size: 30 165 30 726 30 5377
Fragments arranged by size:
5377 726 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6358
BseYI C'CCAG_C
Cuts at: 2487 2607 5561 6057 2487
Size: 120 2954 496 2788
Fragments arranged by size:
2954 2788 496 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2094 1163
Size: 573 358 5427
Fragments arranged by size:
5427 573 358
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2766 3808 4569 5597 1540
Size: 275 186 765 1042 761 1028 2301
Fragments arranged by size:
2301 1042 1028 765 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4419 4504 5575 4419
Size: 85 1071 5202
Fragments arranged by size:
5202 1071 85
BsmI GAATG_Cn'
Cuts at: 3869 3962 3869
Size: 93 6265
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2718 2832 3345 3677 4419 4504
Size: 399 352 576 114 513 332 742 85
Cuts at: 4504 5575 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 576 513 399 352 332
114 85
BspEI T'CCGG_A
Cuts at: 6021 6021
Size: 6358
BspHI T'CATG_A
Cuts at: 3645 4247 5977 3645
Size: 602 1730 4026
Fragments arranged by size:
4026 1730 602
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3713 844
Size: 2869 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4245 1394
Size: 429 2422 3507
Fragments arranged by size:
3507 2422 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3294 3528 4853 5027 65
Size: 1183 2046 234 1325 174 1396
Fragments arranged by size:
2046 1396 1325 1183 234 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3189 5002 5189 1700
Size: 110 1379 1813 187 2869
Fragments arranged by size:
2869 1813 1379 187 110
BsrGI T'GTAC_A
Cuts at: 96 2212 3511 96
Size: 2116 1299 2943
Fragments arranged by size:
2943 2116 1299
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6358
BssSI C'ACGA_G
Cuts at: 4418 5430 4418
Size: 1012 5346
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6358
BstBI TT'CG_AA
Cuts at: 5162 5162
Size: 6358
BstEII G'GTnAC_C
Cuts at: 1184 3226 1184
Size: 2042 4316
BstXI CCAn_nnnn'nTGG
Cuts at: 2940 3301 2940
Size: 361 5997
BstYI r'GATC_y
Cuts at: 1972 3115 3205 3688 4055 4440 4457 5909 5995
Size: 1143 90 483 367 385 17 1452 86
Cuts at: 5995 6007 6353 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1143 483 385 367 346 90
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3493 3493
Size: 6358
BtgI C'CryG_G
Cuts at: 513 1065 2961 3181 3327 513
Size: 552 1896 220 146 3544
Fragments arranged by size:
3544 1896 552 220 146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5619
Fragments arranged by size:
5619 683 56
BtsI GCAGTG_nn'
Cuts at: 2071 3874 4668 4688 2071
Size: 1803 794 20 3741
Fragments arranged by size:
3741 1803 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6358
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6323
DraI TTT'AAA
Cuts at: 2229 3662 2229
Size: 1433 4925
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5365 809
Size: 507 4049 1802
Fragments arranged by size:
4049 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3170 3279 3805
Size: 54 1425 50 275 18 1340 109 526
Cuts at: 3805 4694 6323 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1340 889 526 275 109 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3805 1537
Size: 275 1993 4090
Fragments arranged by size:
4090 1993 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4288 1353
Size: 2935 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4945 5319 5465 387
Size: 1064 138 3356 374 146 1280
Fragments arranged by size:
3356 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2390 2715 3629 4421 5018 5805 6073
Size: 393 758 325 914 792 597 787 268
Cuts at: 6073 1239
Size: 1524
Fragments arranged by size:
1524 914 792 787 758 597 393 325
268
EcoO109I rG'GnC_Cy
Cuts at: 2098 2443 2945 3250 2098
Size: 345 502 305 5206
Fragments arranged by size:
5206 502 345 305
EcoRI G'AATT_C
Cuts at: 3667 4045 3667
Size: 378 5980
FspI TGC'GCA
Cuts at: 4864 4864
Size: 6358
HaeII r_GCGC'y
Cuts at: 1678 5505 1678
Size: 3827 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2513 2545 3437 3469 4999
Size: 32 135 32 941 32 892 32 1530
Cuts at: 4999 5031 5073 5105 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1530 941 892 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2308 2374 3702 3948 669
Size: 988 281 370 66 1328 246 3079
Fragments arranged by size:
3079 1328 988 370 281 246 66
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6358
HpaI GTT'AAC
Cuts at: 2374 3948 2374
Size: 1574 4784
KpnI G_GTAC'C
Cuts at: 2969 3683 2969
Size: 714 5644
MfeI C'AATT_G
Cuts at: 3957 3957
Size: 6358
MluI A'CGCG_T
Cuts at: 6234 6234
Size: 6358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2035 5472 5656 1305
Size: 730 3437 184 2007
Fragments arranged by size:
3437 2007 730 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3172 3281 10
Size: 54 1425 343 1340 109 3087
Fragments arranged by size:
3087 1425 1340 343 109 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3428 4316 4675 4834 518
Size: 648 2262 888 359 159 2042
Fragments arranged by size:
2262 2042 888 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3774 4451 5599 5844 1606
Size: 2168 677 1148 245 2120
Fragments arranged by size:
2168 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2961 3181 3327 513
Size: 552 1896 220 146 3544
Fragments arranged by size:
3544 1896 552 220 146
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6358
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6358
NotI GC'GGCC_GC
Cuts at: 3805 3805
Size: 6358
NsiI A_TGCA'T
Cuts at: 3097 3408 3097
Size: 311 6047
NspI r_CATG'y
Cuts at: 1132 1229 3167 3410 3427 3716 5261 1132
Size: 97 1938 243 17 289 1545 2229
Fragments arranged by size:
2229 1938 1545 289 243 97 17
PasI CC'CwG_GG
Cuts at: 1348 2753 1348
Size: 1405 4953
PciI A'CATG_T
Cuts at: 3163 5257 3163
Size: 2094 4264
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6358
PfoI T'CCnGG_A
Cuts at: 2692 2692
Size: 6358
PmeI GTTT'AAAC
Cuts at: 3662 3662
Size: 6358
PmlI CAC'GTG
Cuts at: 2262 2262
Size: 6358
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2129 2161 4427 4459 5940 5972 2129
Size: 32 2266 32 1481 32 2515
Fragments arranged by size:
2515 2266 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2098 3250 2098
Size: 1152 5206
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6358
PsiI TTA'TAA
Cuts at: 3928 3928
Size: 6358
PstI C_TGCA'G
Cuts at: 830 3710 830
Size: 2880 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6358
SalI G'TCGA_C
Cuts at: 1936 3700 1936
Size: 1764 4594
SbfI CC_TGCA'GG
Cuts at: 3710 3710
Size: 6358
ScaI AGT'ACT
Cuts at: 3355 4606 6318 3355
Size: 1251 1712 3395
Fragments arranged by size:
3395 1712 1251
SfcI C'TryA_G
Cuts at: 826 1045 3566 3706 4841 5522 5713 826
Size: 219 2521 140 1135 681 191 1471
Fragments arranged by size:
2521 1471 1135 681 219 191 140
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6358
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6358
SmaI CCC'GGG
Cuts at: 1872 3685 1872
Size: 1813 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2473 4462 5363 5625 820
Size: 197 926 530 1989 901 262 1553
Fragments arranged by size:
1989 1553 926 901 530 262 197
SnaBI TAC'GTA
Cuts at: 493 4149 493
Size: 3656 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6358
SphI G_CATG'C
Cuts at: 1132 3410 3427 3716 1132
Size: 2278 17 289 3774
Fragments arranged by size:
3774 2278 289 17
SspI AAT'ATT
Cuts at: 5 52 4282 5
Size: 47 4230 2081
Fragments arranged by size:
4230 2081 47
StuI AGG'CCT
Cuts at: 1517 3375 1517
Size: 1858 4500
StyI C'CwwG_G
Cuts at: 513 1065 2740 2961 3181 3327 3797 513
Size: 552 1675 221 220 146 470 3074
Fragments arranged by size:
3074 1675 552 470 221 220 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2033 2310 3455 3470 4385 4402 4555
Size: 1255 453 277 1145 15 915 17 153
Cuts at: 4555 4740 5185 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1145 915 453 445 277 185
153 17 15
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2212 3353 3511
Size: 274 80 33 51 1443 235 1141 158
Cuts at: 3511 4604 6316 96
Size: 1093 1712 138
Fragments arranged by size:
1712 1443 1141 1093 274 235 158 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2667 2699 2790 2822 6318 6350 2667
Size: 32 91 32 3496 32 2675
Fragments arranged by size:
3496 2675 91 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6358
XbaI T'CTAG_A
Cuts at: 3694 3694
Size: 6358
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2130 2266 3274 2130
Size: 136 1008 5214
Fragments arranged by size:
5214 1008 136
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6358
XmaI C'CCGG_G
Cuts at: 1870 3683 1870
Size: 1813 4545
XmnI GAAnn'nnTTC
Cuts at: 2890 4487 2890
Size: 1597 4761
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2862 4166 276
Size: 53 83 186 936 1328 1304 2468
Fragments arranged by size:
2468 1328 1304 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BsiWI BsmBI
BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI