(Linear) (Six-base) MAPSORT of: KIEE3249.seq Check: 1998 from: 1 to: 1653 KIEE3249.orf With 182 enzymes: SgfI * March 22, 2012 16:27 .. AatII G_ACGT'C Cuts at: 0 859 1653 Size: 859 794 Acc65I G'GTAC_C Cuts at: 0 960 1653 Size: 960 693 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1624 1653 Size: 1624 29 AflIII A'CryG_T Cuts at: 0 256 1158 1653 Size: 256 902 495 Fragments arranged by size: 902 495 256 AgeI A'CCGG_T Cuts at: 0 1184 1653 Size: 1184 469 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1418 1653 Size: 1418 235 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 124 156 1653 Size: 124 32 1497 Fragments arranged by size: 1497 124 32 AlwNI CAG_nnn'CTG Cuts at: 0 617 1653 Size: 617 1036 ApoI r'AATT_y Cuts at: 0 230 1653 Size: 230 1423 AvaI C'yCGr_G Cuts at: 0 1554 1653 Size: 1554 99 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 952 985 1653 Size: 952 33 668 Fragments arranged by size: 952 668 33 BanI G'GyrC_C Cuts at: 0 960 1477 1653 Size: 960 517 176 Fragments arranged by size: 960 517 176 BanII G_rGCy'C Cuts at: 0 137 713 1653 Size: 137 576 940 Fragments arranged by size: 940 576 137 BbsI GAAGACnn'nnnn_ Cuts at: 0 644 878 1653 Size: 644 234 775 Fragments arranged by size: 775 644 234 BbvCI CC'TCA_GC Cuts at: 0 1372 1653 Size: 1372 281 BfrBI ATG'CAT Cuts at: 0 1090 1401 1653 Size: 1090 311 252 Fragments arranged by size: 1090 311 252 BglI GCCn_nnn'nGGC Cuts at: 0 1327 1653 Size: 1327 326 BglII A'GATC_T Cuts at: 0 1200 1653 Size: 1200 453 BlpI GC'TnA_GC Cuts at: 0 1127 1653 Size: 1127 526 Bme1580I G_kGCm'C Cuts at: 0 827 1340 1653 Size: 827 513 313 Fragments arranged by size: 827 513 313 BmrI ACTGGGnnnn_n' Cuts at: 0 132 1653 Size: 132 1521 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 177 1653 Size: 177 1476 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 385 710 1653 Size: 385 325 943 Fragments arranged by size: 943 385 325 Bpu10I CC'TnA_GC Cuts at: 0 1194 1372 1653 Size: 1194 178 281 Fragments arranged by size: 1194 281 178 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 489 1653 Size: 489 1164 BsaI GGTCTCn'nnnn_ Cuts at: 0 376 560 725 1193 1653 Size: 376 184 165 468 460 Fragments arranged by size: 468 460 376 184 165 BsaAI yAC'GTr Cuts at: 0 257 1653 Size: 257 1396 BsaBI GATnn'nnATC Cuts at: 0 320 1653 Size: 320 1333 BsaHI Gr'CG_yC Cuts at: 0 856 932 1653 Size: 856 76 721 Fragments arranged by size: 856 721 76 BsaWI w'CCGG_w Cuts at: 0 1184 1225 1653 Size: 1184 41 428 Fragments arranged by size: 1184 428 41 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 124 154 1653 Size: 124 30 1499 Fragments arranged by size: 1499 124 30 BseYI C'CCAG_C Cuts at: 0 482 602 1653 Size: 482 120 1051 Fragments arranged by size: 1051 482 120 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 89 1653 Size: 89 1564 BsiEI CG_ry'CG Cuts at: 0 761 1653 Size: 761 892 Bsp1286I G_dGCh'C Cuts at: 0 137 713 827 1340 1653 Size: 137 576 114 513 313 Fragments arranged by size: 576 513 313 137 114 BspHI T'CATG_A Cuts at: 0 1640 1653 Size: 1640 13 BsrDI GCAATG_nn' Cuts at: 0 1289 1523 1653 Size: 1289 234 130 Fragments arranged by size: 1289 234 130 BsrFI r'CCGG_y Cuts at: 0 1184 1653 Size: 1184 469 BsrGI T'GTAC_A Cuts at: 0 207 1506 1653 Size: 207 1299 147 Fragments arranged by size: 1299 207 147 BstEII G'GTnAC_C Cuts at: 0 1221 1653 Size: 1221 432 BstXI CCAn_nnnn'nTGG Cuts at: 0 935 1296 1653 Size: 935 361 357 Fragments arranged by size: 935 361 357 BstYI r'GATC_y Cuts at: 0 1110 1200 1653 Size: 1110 90 453 Fragments arranged by size: 1110 453 90 Bsu36I CC'TnA_GG Cuts at: 0 1488 1653 Size: 1488 165 BtgI C'CryG_G Cuts at: 0 956 1176 1322 1653 Size: 956 220 146 331 Fragments arranged by size: 956 331 220 146 BtsI GCAGTG_nn' Cuts at: 0 66 1653 Size: 66 1587 DraI TTT'AAA Cuts at: 0 224 1653 Size: 224 1429 EaeI y'GGCC_r Cuts at: 0 1165 1274 1653 Size: 1165 109 379 Fragments arranged by size: 1165 379 109 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 385 710 1624 1653 Size: 385 325 914 29 Fragments arranged by size: 914 385 325 29 EcoO109I rG'GnC_Cy Cuts at: 0 93 438 940 1245 1653 Size: 93 345 502 305 408 Fragments arranged by size: 502 408 345 305 93 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 508 540 1432 1464 1653 Size: 508 32 892 32 189 Fragments arranged by size: 892 508 189 32 32 HincII GTy'rAC Cuts at: 0 303 369 1653 Size: 303 66 1284 Fragments arranged by size: 1284 303 66 HpaI GTT'AAC Cuts at: 0 369 1653 Size: 369 1284 KpnI G_GTAC'C Cuts at: 0 964 1653 Size: 964 689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 30 1653 Size: 30 1623 MscI TGG'CCA Cuts at: 0 1167 1276 1653 Size: 1167 109 377 Fragments arranged by size: 1167 377 109 MslI CAynn'nnrTG Cuts at: 0 1423 1653 Size: 1423 230 NcoI C'CATG_G Cuts at: 0 956 1176 1322 1653 Size: 956 220 146 331 Fragments arranged by size: 956 331 220 146 NsiI A_TGCA'T Cuts at: 0 1092 1403 1653 Size: 1092 311 250 Fragments arranged by size: 1092 311 250 NspI r_CATG'y Cuts at: 0 1162 1405 1422 1653 Size: 1162 243 17 231 Fragments arranged by size: 1162 243 231 17 PasI CC'CwG_GG Cuts at: 0 748 1653 Size: 748 905 PciI A'CATG_T Cuts at: 0 1158 1653 Size: 1158 495 PfoI T'CCnGG_A Cuts at: 0 687 1653 Size: 687 966 PmlI CAC'GTG Cuts at: 0 257 1653 Size: 257 1396 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 124 156 1653 Size: 124 32 1497 Fragments arranged by size: 1497 124 32 PpuMI rG'GwC_Cy Cuts at: 0 93 1245 1653 Size: 93 1152 408 Fragments arranged by size: 1152 408 93 ScaI AGT'ACT Cuts at: 0 1350 1653 Size: 1350 303 SfcI C'TryA_G Cuts at: 0 1561 1653 Size: 1561 92 SmlI C'TyrA_G Cuts at: 0 468 1653 Size: 468 1185 SphI G_CATG'C Cuts at: 0 1405 1422 1653 Size: 1405 17 231 Fragments arranged by size: 1405 231 17 StuI AGG'CCT Cuts at: 0 1370 1653 Size: 1370 283 StyI C'CwwG_G Cuts at: 0 735 956 1176 1322 1653 Size: 735 221 220 146 331 Fragments arranged by size: 735 331 221 220 146 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 28 305 1450 1465 1653 Size: 28 277 1145 15 188 Fragments arranged by size: 1145 277 188 28 15 TatI w'GTAC_w Cuts at: 0 207 1348 1506 1653 Size: 207 1141 158 147 Fragments arranged by size: 1141 207 158 147 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 662 694 785 817 1653 Size: 662 32 91 32 836 Fragments arranged by size: 836 662 91 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 125 261 1269 1653 Size: 125 136 1008 384 Fragments arranged by size: 1008 384 136 125 XmnI GAAnn'nnTTC Cuts at: 0 885 1653 Size: 885 768 ZraI GAC'GTC Cuts at: 0 857 1653 Size: 857 796 Enzymes that do cut: AatII Acc65I AcuI AflIII AgeI AlfI AloI AlwNI ApoI AvaI BaeI BanI BanII BbsI BbvCI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI Bsp1286I BspHI BsrDI BsrFI BsrGI BstEII BstXI BstYI Bsu36I BtgI BtsI DraI EaeI Eco57MI EcoO109I Hin4I HincII HpaI KpnI MmeI MscI MslI NcoI NsiI NspI PasI PciI PfoI PmlI PpiI PpuMI ScaI SfcI SmlI SphI StuI StyI TaqII TatI TstI XcmI XmnI ZraI Enzymes that do not cut: AarI AccI AclI AfeI AflII AhdI AleI ApaI ApaLI AscI AseI AsiSI AvrII BamHI BbeI BcgI BciVI BclI BmgBI BmtI BseRI BsiHKAI BsiWI BsmI BsmBI BspEI BspMI BsrBI BssHII BssSI BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII KasI MfeI MluI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PflMI PmeI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmaI