Restriction map for
pFN21AE6683
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII AccII AcsI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HpaI KasI KspI
MaeI MaeIII MamI MfeI MflI
MluI MluNI MscI MseI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI PvuII
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI TfiI Tru9I Tsp45I Tsp509I
Tth111I TthHB8I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 806 GGTACC G^GTACC
AccI 97 GTCTAC GT^[AC][GT]AC
AccIII 20 TCCGGA T^CCGGA
AclI 741 AACGTT AA^CGTT
AcyI 524 GACGCC G[GA]^CG[CT]C
AlwI 23 GGATC GGATC(4/5)
AlwNI 325 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 682 CCTCAGG CC^T[ACGT]AGG
ApaI 567 GGGCCC GGGCC^C
Asp700I 12 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 806 GGTACC G^GTACC
BglI 574 GCCCCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 524 GACGCC G[GA]^CG[CT]C
BsaWI 20 TCCGGA [AT]^CCGG[AT]
BseAI 20 TCCGGA T^CCGGA
BspEI 20 TCCGGA T^CCGGA
BspMI 692 GCAGGT ACCTGC(4/8)
BsrBI 384 CCGCTC CCGCTC(-3/-3)
Bsu36I 682 CCTCAGG CC^T[ACGT]AGG
Eco81I 682 CCTCAGG CC^T[ACGT]AGG
EcoNI 290 CCTATGTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 635 ATGCAT ATGCA^T
FauI 699 CCCGC CCCGC(4/6)
Hin1I 524 GACGCC G[GA]^CG[CT]C
HindIII 659 AAGCTT A^AGCTT
KpnI 806 GGTACC GGTAC^C
MroI 20 TCCGGA T^CCGGA
MslI 348 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 635 ATGCAT ATGCA^T
NspI 155 GCATGT [GA]CATG^[CT]
PflMI 791 CCAACAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 911 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 741 AACGTT AA^CGTT
PstI 690 CTGCAG CTGCA^G
SapI 267 GAAGAGC GCTCTTC(1/4)
StuI 464 AGGCCT AGG^CCT
Van91I 791 CCAACAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 230 CCATGGGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 12 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
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BbsI 12 GAAGAC GAAGAC(2/6)
877 GAAGAC
BpuAI 12 GAAGAC GAAGAC(2/6)
877 GAAGAC
BspHI 74 TCATGA T^CATGA
362 TCATGA
EarI 267 GAAGAG CTCTTC(1/4)
871 GAAGAG
HgaI 524 GACGC GACGC(5/10)
125 GCGTC
HinfI 196 GACTC G^A[ACGT]TC
610 GACTC
HphI 132 GGTGA GGTGA(8/7)
179 TCACC
Ksp632I 267 GAAGAG CTCTTC(1/4)
871 GAAGAG
MspA1I 700 CCGCTG C[AC]G^C[GT]G
802 CAGCGG
NcoI 116 CCATGG C^CATGG
230 CCATGG
PleI 196 GACTC GAGTC(4/5)
610 GACTC
RcaI 74 TCATGA T^CATGA
362 TCATGA
SfcI 369 CTACAG C^T[GA][CT]AG
690 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
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AciI AfaI AluI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HpaII ItaI
MaeII MboI MboII MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TseI TspRI