Restriction map for
pFN21AE6683
(Circular) (Six-base) MAPSORT of: pFN21AE6683.seq Check: 351 from: 1 to: 5665
pFN21AE6683 5665 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2688 2688
Size: 5665
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3475 278
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2103 3008 1937
Size: 166 905 4594
Fragments arranged by size:
4594 905 166
Acc65I G'GTAC_C
Cuts at: 2811 2986 2811
Size: 175 5490
AclI AA'CG_TT
Cuts at: 1563 2747 3792 4165 1563
Size: 1184 1045 373 3063
Fragments arranged by size:
3063 1184 1045 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2329 3728 5112 5380 1632
Size: 697 1399 1384 268 1917
Fragments arranged by size:
1917 1399 1384 697 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5468
AflIII A'CryG_T
Cuts at: 1203 4564 5541 1203
Size: 3361 977 1327
Fragments arranged by size:
3361 1327 977
AgeI A'CCGG_T
Cuts at: 4496 4496
Size: 5665
AhdI GACnn_n'nnGTC
Cuts at: 4394 4394
Size: 5665
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5665
AlwNI CAG_nnn'CTG
Cuts at: 2335 3408 4980 2335
Size: 1073 1572 3020
Fragments arranged by size:
3020 1572 1073
ApaI G_GGCC'C
Cuts at: 2576 2576
Size: 5665
ApaLI G'TGCA_C
Cuts at: 3722 4878 3722
Size: 1156 4509
ApoI r'AATT_y
Cuts at: 1493 2974 3200 3352 5461 1493
Size: 1481 226 152 2109 1697
Fragments arranged by size:
2109 1697 1481 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4219 160
Size: 872 3187 1606
Fragments arranged by size:
3187 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
AvaI C'yCGr_G
Cuts at: 1870 1943 2990 1870
Size: 73 1047 4545
Fragments arranged by size:
4545 1047 73
BamHI G'GATC_C
Cuts at: 2995 3362 2995
Size: 367 5298
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2108 2700 2811 2986 4441
Size: 325 217 627 321 592 111 175 1455
Cuts at: 4441 618
Size: 1842
Fragments arranged by size:
1842 1455 627 592 325 321 217 175
111
BanII G_rGCy'C
Cuts at: 1391 2279 2507 2576 2763 2984 1391
Size: 888 228 69 187 221 4072
Fragments arranged by size:
4072 888 228 221 187 69
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2024 2889 928
Size: 971 125 865 3704
Fragments arranged by size:
3704 971 865 125
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3856 3890 1903
Size: 34 1919 34 3678
Fragments arranged by size:
3678 1919 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3557 4767 3557
Size: 1210 4455
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5665
BfrBI ATG'CAT
Cuts at: 2642 2642
Size: 5665
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2585 4276 136
Size: 107 122 71 631 605 913 1691 1525
Fragments arranged by size:
1691 1525 913 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5660 5660
Size: 5665
BlpI GC'TnA_GC
Cuts at: 3082 3082
Size: 5665
Bme1580I G_kGCm'C
Cuts at: 1790 2576 2705 3726 4882 1790
Size: 786 129 1021 1156 2573
Fragments arranged by size:
2573 1156 1021 786 129
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5665
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2274 3396 4354
Size: 416 53 83 399 24 857 1122 958
Cuts at: 4354 442
Size: 1753
Fragments arranged by size:
1753 1122 958 857 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2176 2267 2602 4325 1239
Size: 937 91 335 1723 2579
Fragments arranged by size:
2579 1723 937 335 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3790 4655 4953 3790
Size: 865 298 4502
Fragments arranged by size:
4502 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4328 882
Size: 617 2829 2219
Fragments arranged by size:
2829 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3456 493
Size: 711 2252 2702
Fragments arranged by size:
2702 2252 711
BsaBI GATnn'nnATC
Cuts at: 5659 5659
Size: 5665
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2530 3472 3854 4489
Size: 53 83 186 936 997 942 382 635
Cuts at: 4489 275
Size: 1451
Fragments arranged by size:
1451 997 942 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2025 4098 4496 4770 4917 5328 2025
Size: 2073 398 274 147 411 2362
Fragments arranged by size:
2362 2073 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2375 2405 2600 2630 1178
Size: 30 165 30 972 30 195 30 4213
Fragments arranged by size:
4213 972 195 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2457 2846 2912 1186
Size: 1271 389 66 3939
Fragments arranged by size:
3939 1271 389 66
BseYI C'CCAG_C
Cuts at: 2044 2286 2425 2938 4868 5364 2044
Size: 242 139 513 1930 496 2345
Fragments arranged by size:
2345 1930 513 496 242 139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5092
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3115 3876 4904 1540
Size: 275 186 1114 761 1028 2301
Fragments arranged by size:
2301 1114 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3726 3811 4882 3726
Size: 85 1071 4509
Fragments arranged by size:
4509 1071 85
BsmI GAATG_Cn'
Cuts at: 3176 3269 3176
Size: 93 5572
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2279 2507 2576 2705 2763 2984 3726
Size: 399 489 228 69 129 58 221 742
Cuts at: 3726 3811 4882 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 489 399 228 221 129
85 69 58
BspEI T'CCGG_A
Cuts at: 2025 5328 2025
Size: 3303 2362
BspHI T'CATG_A
Cuts at: 2079 2367 3554 5284 2079
Size: 288 1187 1730 2460
Fragments arranged by size:
2460 1730 1187 288
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2688 3020 844
Size: 1844 332 3489
Fragments arranged by size:
3489 1844 332
BsrBI CCG'CTC
Cuts at: 1394 1823 2391 3552 1394
Size: 429 568 1161 3507
Fragments arranged by size:
3507 1161 568 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4160 4334 65
Size: 1183 2912 174 1396
Fragments arranged by size:
2912 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4309 4496 1700
Size: 110 2499 187 2869
Fragments arranged by size:
2869 2499 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5665
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5665
BssSI C'ACGA_G
Cuts at: 3725 4737 3725
Size: 1012 4653
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5665
BstBI TT'CG_AA
Cuts at: 4469 4469
Size: 5665
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5665
BstYI r'GATC_y
Cuts at: 1972 2995 3362 3747 3764 5216 5302 5314 5660
Size: 1023 367 385 17 1452 86 12 346
Cuts at: 5660 1972
Size: 1977
Fragments arranged by size:
1977 1452 1023 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2688 2688
Size: 5665
BtgI C'CryG_G
Cuts at: 513 1065 2004 2121 2235 2487 513
Size: 552 939 117 114 252 3691
Fragments arranged by size:
3691 939 552 252 117 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4926
Fragments arranged by size:
4926 683 56
BtsI GCAGTG_nn'
Cuts at: 2783 3181 3975 3995 2783
Size: 398 794 20 4453
Fragments arranged by size:
4453 794 398 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5665
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5630
DraI TTT'AAA
Cuts at: 2969 2969
Size: 5665
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4672 809
Size: 507 3356 1802
Fragments arranged by size:
3356 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3112 4001 5630
Size: 54 1425 50 275 18 1282 889 1629
Cuts at: 5630 8
Size: 43
Fragments arranged by size:
1629 1425 1282 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3112 1537
Size: 275 1300 4090
Fragments arranged by size:
4090 1300 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2267 2871 3595 1353
Size: 914 604 724 3423
Fragments arranged by size:
3423 914 724 604
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4252 4626 4772 387
Size: 1064 138 2663 374 146 1280
Fragments arranged by size:
2663 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2176 2267 2329 2602 3728 4325 5112
Size: 393 544 91 62 273 1126 597 787
Cuts at: 5112 5380 1239
Size: 268 1524
Fragments arranged by size:
1524 1126 787 597 544 393 273 268
91 62
EcoNI CCTnn'n_nnAGG
Cuts at: 2299 2299
Size: 5665
EcoO109I rG'GnC_Cy
Cuts at: 2572 2573 2917 2572
Size: 1 344 5320
Fragments arranged by size:
5320 344 1
EcoRI G'AATT_C
Cuts at: 2974 3352 2974
Size: 378 5287
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 5665
FspI TGC'GCA
Cuts at: 4171 4171
Size: 5665
HaeII r_GCGC'y
Cuts at: 1678 4812 1678
Size: 3134 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2074 2106 2266 2298 2375
Size: 32 135 32 502 32 160 32 77
Cuts at: 2375 2407 2600 2632 4306 4338 4380 4412 1373
Size: 32 193 32 1674 32 42 32 2626
Fragments arranged by size:
2626 1674 502 193 160 135 77 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3009 3255 669
Size: 988 281 1071 246 3079
Fragments arranged by size:
3079 1071 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2664 748
Size: 1916 3749
HpaI GTT'AAC
Cuts at: 3255 3255
Size: 5665
KpnI G_GTAC'C
Cuts at: 2815 2990 2815
Size: 175 5490
MfeI C'AATT_G
Cuts at: 3264 3264
Size: 5665
MluI A'CGCG_T
Cuts at: 5541 5541
Size: 5665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2730 4779 4963 1305
Size: 1425 2049 184 2007
Fragments arranged by size:
2049 2007 1425 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3843
Fragments arranged by size:
3843 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2357 3623 3982 4141 518
Size: 648 1191 1266 359 159 2042
Fragments arranged by size:
2042 1266 1191 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2707 2809 3081 3758 4906 5151 1606
Size: 1101 102 272 677 1148 245 2120
Fragments arranged by size:
2120 1148 1101 677 272 245 102
NcoI C'CATG_G
Cuts at: 513 1065 2004 2121 2235 513
Size: 552 939 117 114 3943
Fragments arranged by size:
3943 939 552 117 114
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5665
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5665
NotI GC'GGCC_GC
Cuts at: 3112 3112
Size: 5665
NsiI A_TGCA'T
Cuts at: 2644 2644
Size: 5665
NspI r_CATG'y
Cuts at: 1132 1229 2164 3023 4568 1132
Size: 97 935 859 1545 2229
Fragments arranged by size:
2229 1545 935 859 97
PasI CC'CwG_GG
Cuts at: 1348 2569 1348
Size: 1221 4444
PciI A'CATG_T
Cuts at: 4564 4564
Size: 5665
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2802 1108
Size: 1694 3971
PfoI T'CCnGG_A
Cuts at: 2925 2925
Size: 5665
PmeI GTTT'AAAC
Cuts at: 2969 2969
Size: 5665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3734 3766 5247 5279 3734
Size: 32 1481 32 4120
Fragments arranged by size:
4120 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2917 2917
Size: 5665
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5665
PsiI TTA'TAA
Cuts at: 3235 3235
Size: 5665
PspOMI G'GGCC_C
Cuts at: 2572 2572
Size: 5665
PstI C_TGCA'G
Cuts at: 830 2699 3017 830
Size: 1869 318 3478
Fragments arranged by size:
3478 1869 318
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5665
SalI G'TCGA_C
Cuts at: 1936 3007 1936
Size: 1071 4594
SapI GCTCTTCn'nnn_
Cuts at: 2267 2267
Size: 5665
SbfI CC_TGCA'GG
Cuts at: 3017 3017
Size: 5665
ScaI AGT'ACT
Cuts at: 3913 5625 3913
Size: 1712 3953
SfcI C'TryA_G
Cuts at: 826 1045 2374 2695 3013 4148 4829 5020 826
Size: 219 1329 321 318 1135 681 191 1471
Fragments arranged by size:
1471 1329 1135 681 321 318 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5665
SmaI CCC'GGG
Cuts at: 1872 2992 1872
Size: 1120 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3769 4670 4932 820
Size: 197 926 1826 901 262 1553
Fragments arranged by size:
1826 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3456 493
Size: 2963 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5665
SphI G_CATG'C
Cuts at: 1132 3023 1132
Size: 1891 3774
SspI AAT'ATT
Cuts at: 5 52 3589 5
Size: 47 3537 2081
Fragments arranged by size:
3537 2081 47
StuI AGG'CCT
Cuts at: 1517 2471 1517
Size: 954 4711
StyI C'CwwG_G
Cuts at: 513 1065 2004 2121 2235 2583 2816 2959 3104
Size: 552 939 117 114 348 233 143 145
Cuts at: 3104 513
Size: 3074
Fragments arranged by size:
3074 939 552 348 233 145 143 117
114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2972 3692 3709 3862 4047 4492 325
Size: 1255 1392 720 17 153 185 445 1498
Fragments arranged by size:
1498 1392 1255 720 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2889 3911 5623
Size: 274 80 33 51 1443 912 1022 1712
Cuts at: 5623 96
Size: 138
Fragments arranged by size:
1712 1443 1022 912 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5625 5657 5625
Size: 32 5633
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5665
XbaI T'CTAG_A
Cuts at: 3001 3001
Size: 5665
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2242 2242
Size: 5665
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5665
XmaI C'CCGG_G
Cuts at: 1870 2990 1870
Size: 1120 4545
XmnI GAAnn'nnTTC
Cuts at: 2021 3794 2021
Size: 1773 3892
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3473 276
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI