Restriction map for
pFN21AE3683
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BssHII BssSI Bst1107I BstAPI BstEII
BstF5I BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraI DraII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FokI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MslI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NlaIII NlaIV NotI NruI NsiI
NspI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AclI 474 AACGTT AA^CGTT
AcsI 7 AAATTC [GA]^AATT[CT]
ApoI 7 AAATTC [GA]^AATT[CT]
AseI 499 ATTAAT AT^TAAT
AsnI 499 ATTAAT AT^TAAT
BbvI 588 GCTGC GCAGC(8/12)
BclI 156 TGATCA T^GATCA
BcnI 564 CCGGG CC^[GC]GG
BfaI 26 CTAG C^TAG
BglI 507 GCCGTACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
Bsp1407I 606 TGTACA T^GTACA
BsrBI 579 GAGCGG CCGCTC(-3/-3)
BsrFI 512 ACCGGC [GA]^CCGG[CT]
BsrGI 606 TGTACA T^GTACA
BstBI 17 TTCGAA TT^CGAA
Cfr10I 512 ACCGGC [GA]^CCGG[CT]
Cfr13I 505 GGGCC G^G[ACGT]CC
EcoT14I 425 CCTTGG C^C[AT][AT]GG
FauI 491 CCCGC CCCGC(4/6)
FbaI 156 TGATCA T^GATCA
MaeI 26 CTAG C^TAG
MaeIII 245 GTCAC ^GT[ACGT]AC
NciI 564 CCGGG CC^[GC]GG
NspV 17 TTCGAA TT^CGAA
PleI 385 GAGTC GAGTC(4/5)
PshBI 499 ATTAAT AT^TAAT
Psp1406I 474 AACGTT AA^CGTT
SapI 576 GAAGAGC GCTCTTC(1/4)
Sau96I 505 GGGCC G^G[ACGT]CC
SfaNI 261 GATGC GCATC(5/9)
SfuI 17 TTCGAA TT^CGAA
SspBI 606 TGTACA T^GTACA
SspI 460 AATATT AAT^ATT
StyI 425 CCTTGG C^C[AT][AT]GG
TaqI 18 TCGA T^CGA
TseI 588 GCTGC G^C[AT]GC
Tsp45I 245 GTCAC ^GT[GC]AC
TthHB8I 18 TCGA T^CGA
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# TWO CUT ENZYMES ( 29 enzymes) #
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AfaI 510 GTAC GT^AC
607 GTAC
AluI 149 AGCT AG^CT
555 AGCT
AlwI 373 GATCC GGATC(4/5)
441 GGATC
Asp700I 321 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
472 GAAACGTTTC
BanII 527 GAGCCC G[GA]GC[CT]^C
566 GGGCTC
BbsI 118 GAAGAC GAAGAC(2/6)
535 GTCTTC
BmyI 527 GAGCCC G[AGT]GC[ACT]^C
566 GGGCTC
BpuAI 118 GAAGAC GAAGAC(2/6)
535 GTCTTC
BsiYI 513 CCGGCGGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
564 CCGGGCTCAGG
BslI 513 CCGGCGGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
564 CCGGGCTCAGG
Bsp1286I 527 GAGCCC G[AGT]GC[ACT]^C
566 GGGCTC
BsrI 450 ACTGG ACTGG(1/-1)
592 CCAGT
BstNI 215 CCAGG CC^[AT]GG
531 CCTGG
Cac8I 78 GCAGGC GC[ACGT]^[ACGT]GC
584 GCTCGC
DraIII 247 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
312 CACTGAGTG
EarI 99 GAAGAG CTCTTC(1/4)
576 GAAGAG
EcoRII 215 CCAGG ^CC[AT]GG
531 CCTGG
Fnu4HI 581 GCGGC GC^[ACGT]GC
588 GCTGC
HaeIII 81 GGCC GG^CC
506 GGCC
HapII 513 CCGG C^CGG
564 CCGG
HpaII 513 CCGG C^CGG
564 CCGG
ItaI 581 GCGGC GC^[ACGT]GC
588 GCTGC
Ksp632I 99 GAAGAG CTCTTC(1/4)
576 GAAGAG
MaeII 475 ACGT A^CGT
601 ACGT
MspI 513 CCGG C^CGG
564 CCGG
MvaI 215 CCAGG CC^[AT]GG
531 CCTGG
MwoI 289 GCTTCTCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
507 GCCGTACCGGC
RsaI 510 GTAC GT^AC
607 GTAC
XmnI 321 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
472 GAAACGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AciI BpmI BsaJI BssKI DdeI
DpnII HinfI HphI MboI MboII
MnlI MseI NdeII Sau3AI ScrFI
TfiI Tru9I Tsp509I TspRI