(Linear) (Six-base) MAPSORT of: KIEE3683.seq  Check: 8304  from: 1  to: 624



KIEE3683.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:38  ..

AclI AA'CG_TT

Cuts at:      0     475     624 
   Size:        475     149
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     624 
   Size:        118     506
 
ApoI r'AATT_y

Cuts at:      0       7     624 
   Size:          7     617
 
AseI AT'TA_AT

Cuts at:      0     500     624 
   Size:        500     124
 
BanII G_rGCy'C

Cuts at:      0     531     570     624 
   Size:        531      39      54

  Fragments arranged by size: 

                 531      54      39
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     125     528     624 
   Size:        125     403      96

  Fragments arranged by size: 

                 403     125      96
 
BclI T'GATC_A

Cuts at:      0     156     624 
   Size:        156     468
 
BglI GCCn_nnn'nGGC

Cuts at:      0     513     624 
   Size:        513     111
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     166     198     624 
   Size:         80      86      32     426

  Fragments arranged by size: 

                 426      86      80      32
 
Bpu10I CC'TnA_GC

Cuts at:      0     569     624 
   Size:        569      55
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      53      83     624 
   Size:         53      30     541

  Fragments arranged by size: 

                 541      53      30
 
Bsp1286I G_dGCh'C

Cuts at:      0     531     570     624 
   Size:        531      39      54

  Fragments arranged by size: 

                 531      54      39
 
BsrBI CCG'CTC

Cuts at:      0     581     624 
   Size:        581      43
 
BsrFI r'CCGG_y

Cuts at:      0     512     624 
   Size:        512     112
 
BsrGI T'GTAC_A

Cuts at:      0     606     624 
   Size:        606      18
 
BstBI TT'CG_AA

Cuts at:      0      18     624 
   Size:         18     606
 
DraIII CAC_nnn'GTG

Cuts at:      0     252     317     624 
   Size:        252      65     307

  Fragments arranged by size: 

                 307     252      65
 
EarI CTCTTCn'nnn_

Cuts at:      0      94     571     624 
   Size:         94     477      53

  Fragments arranged by size: 

                 477      94      53
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     531     624 
   Size:        531      93
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     118     166     198     624 
   Size:         80      38      48      32     426

  Fragments arranged by size: 

                 426      80      48      38      32
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     190     624 
   Size:        190     434
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      78     110     518     545     550     577     624 
   Size:         78      32     408      27       5      27      47

  Fragments arranged by size: 

                 408      78      47      32      27      27       5
 
SapI GCTCTTCn'nnn_

Cuts at:      0     571     624 
   Size:        571      53
 
SspI AAT'ATT

Cuts at:      0     462     624 
   Size:        462     162
 
StyI C'CwwG_G

Cuts at:      0     425     624 
   Size:        425     199
 
TatI w'GTAC_w

Cuts at:      0     606     624 
   Size:        606      18
 
XmnI GAAnn'nnTTC

Cuts at:      0     325     476     624 
   Size:        325     151     148

  Fragments arranged by size: 

                 325     151     148
 

 Enzymes that do cut:

     AclI     AcuI     ApoI     AseI    BanII     BbsI     BclI     BglI
     BpmI   Bpu10I    BsaXI Bsp1286I    BsrBI    BsrFI    BsrGI    BstBI
   DraIII     EarI     EciI  Eco57MI     FalI    Hin4I     SapI     SspI
     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrDI   BssHII    BssSI   BstAPI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI