Restriction map for
pFN21AB7679
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI AlwI
AlwNI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BalI BamHI BbeI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsgI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
DraI DraIII DrdI Eam1105I EarI
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KasI KpnI Ksp632I
MaeIII MamI MfeI MflI MluI
MluNI MscI MseI MslI MunI
NaeI NarI NdeI NgoMIV NheI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI PvuII RcaI SacI
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp45I Tsp509I Tth111I
Van91I XbaI XcmI XhoII XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 310 TCCGGA T^CCGGA
AluI 781 AGCT AG^CT
Alw44I 605 GTGCAC G^TGCAC
ApaLI 605 GTGCAC G^TGCAC
BanI 60 GGTGCC G^G[CT][GA]CC
BbrPI 427 CACGTG CAC^GTG
BfaI 523 CTAG C^TAG
BsaAI 427 CACGTG [CT]AC^GT[GA]
BsaWI 310 TCCGGA [AT]^CCGG[AT]
BseAI 310 TCCGGA T^CCGGA
BspEI 310 TCCGGA T^CCGGA
BsrFI 405 GCCGGT [GA]^CCGG[CT]
BstAPI 156 GCAGAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 969 GGATG GGATG(2/0)
Cfr10I 405 GCCGGT [GA]^CCGG[CT]
EcoNI 696 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 969 GGATG GGATG(9/13)
HaeII 134 AGCGCC [GA]GCGC^[CT]
HinfI 303 GACTC G^A[ACGT]TC
KspI 370 CCGCGG CCGC^GG
MaeI 523 CTAG C^TAG
MaeII 428 ACGT A^CGT
MroI 310 TCCGGA T^CCGGA
NcoI 48 CCATGG C^CATGG
PaeR7I 33 CTCGAG C^TCGAG
PleI 303 GACTC GAGTC(4/5)
PmaCI 427 CACGTG CAC^GTG
PmlI 427 CACGTG CAC^GTG
PstI 697 CTGCAG CTGCA^G
SacII 370 CCGCGG CCGC^GG
SfcI 697 CTGCAG C^T[GA][CT]AG
SphI 638 GCATGC GCATG^C
StuI 227 AGGCCT AGG^CCT
XhoI 33 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
ApaI 217 GGGCCC GGGCC^C
612 GGGCCC
BglI 229 GCCTCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
448 GCCGCCGTGGC
BpmI 400 CTCCAG CTGGAG(16/14)
633 CTCCAG
BsmBI 1007 CGTCTC CGTCTC(1/5)
314 GAGACG
BspMI 527 ACCTGC ACCTGC(4/8)
788 ACCTGC
CpoI 544 CGGACCG CG^G[AT]CCG
599 CGGACCG
DdeI 617 CTGAG C^T[ACGT]AG
1010 CTCAG
DpnII 126 GATC ^GATC
144 GATC
EcoT14I 48 CCATGG C^C[AT][AT]GG
735 CCAAGG
HphI 988 GGTGA GGTGA(8/7)
566 TCACC
MboI 126 GATC ^GATC
144 GATC
MboII 141 GAAGA GAAGA(8/7)
356 TCTTC
NdeII 126 GATC ^GATC
144 GATC
NspI 638 GCATGC [GA]CATG^[CT]
714 GCATGT
PpuMI 327 GGGACCC [GA]G^G[AT]CC[CT]
474 GGGACCC
RsrII 544 CGGACCG CG^G[AT]CCG
599 CGGACCG
Sau3AI 126 GATC ^GATC
144 GATC
SmaI 297 CCCGGG CCC^GGG
324 CCCGGG
SmlI 33 CTCGAG C^T[CT][GA]AG
166 CTCAAG
StyI 48 CCATGG C^C[AT][AT]GG
735 CCAAGG
TaqI 34 TCGA T^CGA
359 TCGA
TthHB8I 34 TCGA T^CGA
359 TCGA
XmaCI 297 CCCGGG C^CCGGG
324 CCCGGG
XmaI 297 CCCGGG C^CCGGG
324 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AcyI AfaI AspHI
AvaI AvaII AviII BanII BbvI
BcnI BmyI BsaHI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DraII DsaI EaeI EagI EclXI
Eco52I EcoO109I EcoRII FauI Fnu4HI
FspI HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII ItaI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV NotI RsaI
Sau96I ScrFI SfaNI TseI TspRI