(Circular) (Six-base) MAPSORT of: pFN21AB7679.seq Check: 1436 from: 1 to: 5731 pFN21AB7679 5731 bp With 182 enzymes: SgfI * March 28, 2012 12:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3541 278 Size: 53 83 186 936 2005 2468 Fragments arranged by size: 2468 2005 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3074 1937 Size: 1137 4594 Acc65I G'GTAC_C Cuts at: 3052 3052 Size: 5731 AclI AA'CG_TT Cuts at: 1563 3858 4231 1563 Size: 2295 373 3063 Fragments arranged by size: 3063 2295 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3794 5178 5446 1632 Size: 2162 1384 268 1917 Fragments arranged by size: 2162 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5534 AflIII A'CryG_T Cuts at: 1203 4630 5607 1203 Size: 3427 977 1327 Fragments arranged by size: 3427 1327 977 AgeI A'CCGG_T Cuts at: 4562 4562 Size: 5731 AhdI GACnn_n'nnGTC Cuts at: 4460 4460 Size: 5731 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5731 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2561 1184 Size: 1377 4354 AlwNI CAG_nnn'CTG Cuts at: 3474 5046 3474 Size: 1572 4159 ApaI G_GGCC'C Cuts at: 2226 2621 2226 Size: 395 5336 ApaLI G'TGCA_C Cuts at: 2610 3788 4944 2610 Size: 1178 1156 3397 Fragments arranged by size: 3397 1178 1156 ApoI r'AATT_y Cuts at: 1493 3040 3266 3418 5527 1493 Size: 1547 226 152 2109 1697 Fragments arranged by size: 2109 1697 1547 226 152 AseI AT'TA_AT Cuts at: 160 1032 4285 160 Size: 872 3253 1606 Fragments arranged by size: 3253 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5731 AvaI C'yCGr_G Cuts at: 1870 1943 2038 2302 2329 2336 2395 3056 1870 Size: 73 95 264 27 7 59 661 4545 Fragments arranged by size: 4545 661 264 95 73 59 27 7 BamHI G'GATC_C Cuts at: 3061 3428 3061 Size: 367 5364 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2065 3052 4507 618 Size: 325 217 627 278 987 1455 1842 Fragments arranged by size: 1842 1455 987 627 325 278 217 BanII G_rGCy'C Cuts at: 1391 2226 2404 2621 3000 3050 1391 Size: 835 178 217 379 50 4072 Fragments arranged by size: 4072 835 379 217 178 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4760 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3922 3956 1903 Size: 34 1985 34 3678 Fragments arranged by size: 3678 1985 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3623 4833 3623 Size: 1210 4521 BclI T'GATC_A Cuts at: 1548 1548 Size: 5731 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2240 2459 4342 Size: 107 122 71 631 605 568 219 1883 Cuts at: 4342 136 Size: 1525 Fragments arranged by size: 1883 1525 631 605 568 219 122 107 71 BglII A'GATC_T Cuts at: 5726 5726 Size: 5731 BlpI GC'TnA_GC Cuts at: 3148 3148 Size: 5731 Bme1580I G_kGCm'C Cuts at: 1790 2070 2226 2559 2614 2621 3792 4948 1790 Size: 280 156 333 55 7 1171 1156 2573 Fragments arranged by size: 2573 1171 1156 333 280 156 55 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5731 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2624 2842 3462 Size: 416 53 83 399 24 1207 218 620 Cuts at: 3462 4420 442 Size: 958 1753 Fragments arranged by size: 1753 1207 958 620 416 399 218 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5731 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2390 2623 4391 1239 Size: 1151 233 1768 2579 Fragments arranged by size: 2579 1768 1151 233 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2156 3856 4721 5019 2156 Size: 1700 865 298 2868 Fragments arranged by size: 2868 1700 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4394 882 Size: 617 2895 2219 Fragments arranged by size: 2895 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2434 3522 493 Size: 711 1230 1088 2702 Fragments arranged by size: 2702 1230 1088 711 BsaBI GATnn'nnATC Cuts at: 5725 5725 Size: 5731 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2322 2682 2694 3538 Size: 53 83 186 936 789 360 12 844 Cuts at: 3538 3920 4555 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 844 789 635 382 360 186 83 53 12 BsaWI w'CCGG_w Cuts at: 2315 4164 4562 4836 4983 5394 2315 Size: 1849 398 274 147 411 2652 Fragments arranged by size: 2652 1849 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2117 2147 2739 2769 1178 Size: 30 165 30 714 30 592 30 4140 Fragments arranged by size: 4140 714 592 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2204 2207 2784 1186 Size: 1018 3 577 4133 Fragments arranged by size: 4133 1018 577 3 BseYI C'CCAG_C Cuts at: 2468 2668 2999 4934 5430 2468 Size: 200 331 1935 496 2769 Fragments arranged by size: 2769 1935 496 331 200 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5158 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2250 2280 2352 2425 3181 3942 Size: 275 186 249 30 72 73 756 761 Cuts at: 3942 4970 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 756 275 249 186 73 72 30 BsiHKAI G_wGCw'C Cuts at: 2039 2172 2614 3792 3877 4948 2039 Size: 133 442 1178 85 1071 2822 Fragments arranged by size: 2822 1178 1071 442 133 85 BsmI GAATG_Cn' Cuts at: 3242 3335 3242 Size: 93 5638 BsmBI CGTCTCn'nnnn_ Cuts at: 2313 3018 2313 Size: 705 5026 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2039 2070 2172 2226 2404 2559 2614 Size: 399 249 31 102 54 178 155 55 Cuts at: 2614 2621 3000 3050 3792 3877 4948 1391 Size: 7 379 50 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 379 249 178 155 102 85 55 54 50 31 7 BspEI T'CCGG_A Cuts at: 2315 5394 2315 Size: 3079 2652 BspHI T'CATG_A Cuts at: 3620 5350 3620 Size: 1730 4001 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2541 2802 3086 844 Size: 1697 261 284 3489 Fragments arranged by size: 3489 1697 284 261 BsrBI CCG'CTC Cuts at: 1394 1823 3618 1394 Size: 429 1795 3507 Fragments arranged by size: 3507 1795 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4226 4400 65 Size: 1183 2978 174 1396 Fragments arranged by size: 2978 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2410 4375 4562 1700 Size: 110 600 1965 187 2869 Fragments arranged by size: 2869 1965 600 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5731 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5731 BssSI C'ACGA_G Cuts at: 3791 4803 3791 Size: 1012 4719 BstAPI GCAn_nnn'nTGC Cuts at: 794 2167 794 Size: 1373 4358 BstBI TT'CG_AA Cuts at: 4535 4535 Size: 5731 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5731 BstYI r'GATC_y Cuts at: 1972 3061 3428 3813 3830 5282 5368 5380 5726 Size: 1089 367 385 17 1452 86 12 346 Cuts at: 5726 1972 Size: 1977 Fragments arranged by size: 1977 1452 1089 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2053 2375 2457 513 Size: 552 939 49 322 82 3787 Fragments arranged by size: 3787 939 552 322 82 49 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2477 487 Size: 683 56 1251 3741 Fragments arranged by size: 3741 1251 683 56 BtsI GCAGTG_nn' Cuts at: 2111 2515 2813 3247 4041 4061 2111 Size: 404 298 434 794 20 3781 Fragments arranged by size: 3781 794 434 404 298 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5731 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5696 DraI TTT'AAA Cuts at: 3035 3035 Size: 5731 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4738 809 Size: 507 3422 1802 Fragments arranged by size: 3422 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2154 2247 Size: 54 1425 50 275 18 177 147 93 Cuts at: 2247 2277 2349 2422 3178 4067 5696 8 Size: 30 72 73 756 889 1629 43 Fragments arranged by size: 1629 1425 889 756 275 177 147 93 73 72 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2247 2277 2349 2422 3178 1537 Size: 275 435 30 72 73 756 4090 Fragments arranged by size: 4090 756 435 275 73 72 30 EarI CTCTTCn'nnn_ Cuts at: 1353 3661 1353 Size: 2308 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2279 2510 2760 4318 4692 4838 Size: 1064 138 690 231 250 1558 374 146 Cuts at: 4838 387 Size: 1280 Fragments arranged by size: 1558 1280 1064 690 374 250 231 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2390 2623 3794 4391 5178 5446 1239 Size: 393 758 233 1171 597 787 268 1524 Fragments arranged by size: 1524 1171 787 758 597 393 268 233 EcoNI CCTnn'n_nnAGG Cuts at: 2705 2705 Size: 5731 EcoO109I rG'GnC_Cy Cuts at: 2222 2333 2480 2618 2222 Size: 111 147 138 5335 Fragments arranged by size: 5335 147 138 111 EcoRI G'AATT_C Cuts at: 3040 3418 3040 Size: 378 5353 FspI TGC'GCA Cuts at: 2024 2217 2537 4237 2024 Size: 193 320 1700 3518 Fragments arranged by size: 3518 1700 320 193 FspAI rTGC'GCAy Cuts at: 2024 2024 Size: 5731 HaeII r_GCGC'y Cuts at: 1678 2143 4878 1678 Size: 465 2735 2531 Fragments arranged by size: 2735 2531 465 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2972 3004 4372 4404 4446 Size: 32 135 32 1400 32 1368 32 42 Cuts at: 4446 4478 1373 Size: 32 2626 Fragments arranged by size: 2626 1400 1368 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3075 3321 669 Size: 988 281 1137 246 3079 Fragments arranged by size: 3079 1137 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5731 HpaI GTT'AAC Cuts at: 3321 3321 Size: 5731 KpnI G_GTAC'C Cuts at: 3056 3056 Size: 5731 MfeI C'AATT_G Cuts at: 3330 3330 Size: 5731 MluI A'CGCG_T Cuts at: 5607 5607 Size: 5731 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3051 4845 5029 1305 Size: 1746 1794 184 2007 Fragments arranged by size: 2007 1794 1746 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3909 Fragments arranged by size: 3909 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3689 4048 4207 518 Size: 648 2523 359 159 2042 Fragments arranged by size: 2523 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2097 2296 2377 2668 3147 3824 4972 5217 Size: 491 199 81 291 479 677 1148 245 Cuts at: 5217 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 491 479 291 245 199 81 NcoI C'CATG_G Cuts at: 513 1065 2004 2053 513 Size: 552 939 49 4191 Fragments arranged by size: 4191 939 552 49 NdeI CA'TA_TG Cuts at: 387 387 Size: 5731 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5731 NotI GC'GGCC_GC Cuts at: 2247 2349 2422 3178 2247 Size: 102 73 756 4800 Fragments arranged by size: 4800 756 102 73 NspI r_CATG'y Cuts at: 1132 1229 2647 2723 3089 4634 1132 Size: 97 1418 76 366 1545 2229 Fragments arranged by size: 2229 1545 1418 366 97 76 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5731 PciI A'CATG_T Cuts at: 4630 4630 Size: 5731 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5731 PmeI GTTT'AAAC Cuts at: 3035 3035 Size: 5731 PmlI CAC'GTG Cuts at: 2434 2434 Size: 5731 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3800 3832 5313 5345 3800 Size: 32 1481 32 4186 Fragments arranged by size: 4186 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2333 2480 2333 Size: 147 5584 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5731 PsiI TTA'TAA Cuts at: 3301 3301 Size: 5731 PspOMI G'GGCC_C Cuts at: 2222 2617 2222 Size: 395 5336 PstI C_TGCA'G Cuts at: 830 2706 3083 830 Size: 1876 377 3478 Fragments arranged by size: 3478 1876 377 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5731 RsrII CG'GwC_CG Cuts at: 2550 2605 2550 Size: 55 5676 SacII CC_GC'GG Cuts at: 2378 2378 Size: 5731 SalI G'TCGA_C Cuts at: 1936 3073 1936 Size: 1137 4594 SanDI GG'GwC_CC Cuts at: 2333 2480 2333 Size: 147 5584 SbfI CC_TGCA'GG Cuts at: 3083 3083 Size: 5731 ScaI AGT'ACT Cuts at: 3979 5691 3979 Size: 1712 4019 SfcI C'TryA_G Cuts at: 826 1045 2702 3079 4214 4895 5086 826 Size: 219 1657 377 1135 681 191 1471 Fragments arranged by size: 1657 1471 1135 681 377 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5731 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5731 SmaI CCC'GGG Cuts at: 1872 2304 2331 3058 1872 Size: 432 27 727 4545 Fragments arranged by size: 4545 727 432 27 SmlI C'TyrA_G Cuts at: 820 1017 1943 2038 2171 3835 4736 4998 820 Size: 197 926 95 133 1664 901 262 1553 Fragments arranged by size: 1664 1553 926 901 262 197 133 95 SnaBI TAC'GTA Cuts at: 493 3522 493 Size: 3029 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5731 SphI G_CATG'C Cuts at: 1132 2647 3089 1132 Size: 1515 442 3774 Fragments arranged by size: 3774 1515 442 SspI AAT'ATT Cuts at: 5 52 3655 5 Size: 47 3603 2081 Fragments arranged by size: 3603 2081 47 StuI AGG'CCT Cuts at: 1517 2234 1517 Size: 717 5014 StyI C'CwwG_G Cuts at: 513 1065 2004 2053 2740 3170 513 Size: 552 939 49 687 430 3074 Fragments arranged by size: 3074 939 687 552 430 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3758 3775 3928 4113 4558 325 Size: 1255 2178 17 153 185 445 1498 Fragments arranged by size: 2178 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3977 5689 96 Size: 274 80 33 51 1443 2000 1712 138 Fragments arranged by size: 2000 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2739 2771 2919 2951 5691 5723 2739 Size: 32 148 32 2740 32 2747 Fragments arranged by size: 2747 2740 148 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5731 XbaI T'CTAG_A Cuts at: 3067 3067 Size: 5731 XhoI C'TCGA_G Cuts at: 1943 2038 1943 Size: 95 5636 XmaI C'CCGG_G Cuts at: 1870 2302 2329 3056 1870 Size: 432 27 727 4545 Fragments arranged by size: 4545 727 432 27 XmnI GAAnn'nnTTC Cuts at: 3860 3860 Size: 5731 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3539 276 Size: 53 83 186 936 2005 2468 Fragments arranged by size: 2468 2005 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI RsrII SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI PvuII SacI SapI SexAI SfiI SfoI SrfI SwaI XcmI