(Linear) (Six-base) MAPSORT of: KIBB7679.seq Check: 5845 from: 1 to: 1026 KIBB7679.orf With 182 enzymes: SgfI * March 22, 2012 15:39 .. AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 556 1026 Size: 556 470 ApaI G_GGCC'C Cuts at: 0 221 616 1026 Size: 221 395 410 Fragments arranged by size: 410 395 221 ApaLI G'TGCA_C Cuts at: 0 605 1026 Size: 605 421 AvaI C'yCGr_G Cuts at: 0 33 297 324 331 390 1026 Size: 33 264 27 7 59 636 Fragments arranged by size: 636 264 59 33 27 7 BanI G'GyrC_C Cuts at: 0 60 1026 Size: 60 966 BanII G_rGCy'C Cuts at: 0 221 399 616 995 1026 Size: 221 178 217 379 31 Fragments arranged by size: 379 221 217 178 31 BglI GCCn_nnn'nGGC Cuts at: 0 235 454 1026 Size: 235 219 572 Fragments arranged by size: 572 235 219 Bme1580I G_kGCm'C Cuts at: 0 65 221 554 609 616 1026 Size: 65 156 333 55 7 410 Fragments arranged by size: 410 333 156 65 55 7 BmrI ACTGGGnnnn_n' Cuts at: 0 619 837 1026 Size: 619 218 189 Fragments arranged by size: 619 218 189 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 385 618 1026 Size: 385 233 408 Fragments arranged by size: 408 385 233 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 1026 Size: 151 875 BsaAI yAC'GTr Cuts at: 0 429 1026 Size: 429 597 BsaHI Gr'CG_yC Cuts at: 0 317 677 689 1026 Size: 317 360 12 337 Fragments arranged by size: 360 337 317 12 BsaWI w'CCGG_w Cuts at: 0 310 1026 Size: 310 716 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 112 142 734 764 1026 Size: 112 30 592 30 262 Fragments arranged by size: 592 262 112 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 199 202 779 1026 Size: 199 3 577 247 Fragments arranged by size: 577 247 199 3 BseYI C'CCAG_C Cuts at: 0 463 663 994 1026 Size: 463 200 331 32 Fragments arranged by size: 463 331 200 32 BsiEI CG_ry'CG Cuts at: 0 245 275 347 420 1026 Size: 245 30 72 73 606 Fragments arranged by size: 606 245 73 72 30 BsiHKAI G_wGCw'C Cuts at: 0 34 167 609 1026 Size: 34 133 442 417 Fragments arranged by size: 442 417 133 34 BsmBI CGTCTCn'nnnn_ Cuts at: 0 308 1013 1026 Size: 308 705 13 Fragments arranged by size: 705 308 13 Bsp1286I G_dGCh'C Cuts at: 0 34 65 167 221 399 554 609 616 Size: 34 31 102 54 178 155 55 7 Cuts at: 616 995 1026 Size: 379 31 Fragments arranged by size: 379 178 155 102 55 54 34 31 31 7 BspEI T'CCGG_A Cuts at: 0 310 1026 Size: 310 716 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 536 797 1026 Size: 536 261 229 Fragments arranged by size: 536 261 229 BsrFI r'CCGG_y Cuts at: 0 405 1026 Size: 405 621 BstAPI GCAn_nnn'nTGC Cuts at: 0 162 1026 Size: 162 864 BtgI C'CryG_G Cuts at: 0 48 370 452 1026 Size: 48 322 82 574 Fragments arranged by size: 574 322 82 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 472 1026 Size: 472 554 BtsI GCAGTG_nn' Cuts at: 0 106 510 808 1026 Size: 106 404 298 218 Fragments arranged by size: 404 298 218 106 EaeI y'GGCC_r Cuts at: 0 2 149 242 272 344 417 1026 Size: 2 147 93 30 72 73 609 Fragments arranged by size: 609 147 93 73 72 30 2 EagI C'GGCC_G Cuts at: 0 242 272 344 417 1026 Size: 242 30 72 73 609 Fragments arranged by size: 609 242 73 72 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 274 505 755 1026 Size: 274 231 250 271 Fragments arranged by size: 274 271 250 231 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 385 618 1026 Size: 385 233 408 Fragments arranged by size: 408 385 233 EcoNI CCTnn'n_nnAGG Cuts at: 0 700 1026 Size: 700 326 EcoO109I rG'GnC_Cy Cuts at: 0 217 328 475 613 1026 Size: 217 111 147 138 413 Fragments arranged by size: 413 217 147 138 111 FspI TGC'GCA Cuts at: 0 19 212 532 1026 Size: 19 193 320 494 Fragments arranged by size: 494 320 193 19 FspAI rTGC'GCAy Cuts at: 0 19 1026 Size: 19 1007 HaeII r_GCGC'y Cuts at: 0 138 1026 Size: 138 888 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 967 999 1026 Size: 967 32 27 Fragments arranged by size: 967 32 27 MspA1I CmG'CkG Cuts at: 0 92 291 372 663 1026 Size: 92 199 81 291 363 Fragments arranged by size: 363 291 199 92 81 NcoI C'CATG_G Cuts at: 0 48 1026 Size: 48 978 NotI GC'GGCC_GC Cuts at: 0 242 344 417 1026 Size: 242 102 73 609 Fragments arranged by size: 609 242 102 73 NspI r_CATG'y Cuts at: 0 642 718 1026 Size: 642 76 308 Fragments arranged by size: 642 308 76 PmlI CAC'GTG Cuts at: 0 429 1026 Size: 429 597 PpuMI rG'GwC_Cy Cuts at: 0 328 475 1026 Size: 328 147 551 Fragments arranged by size: 551 328 147 PspOMI G'GGCC_C Cuts at: 0 217 612 1026 Size: 217 395 414 Fragments arranged by size: 414 395 217 PstI C_TGCA'G Cuts at: 0 701 1026 Size: 701 325 RsrII CG'GwC_CG Cuts at: 0 545 600 1026 Size: 545 55 426 Fragments arranged by size: 545 426 55 SacII CC_GC'GG Cuts at: 0 373 1026 Size: 373 653 SanDI GG'GwC_CC Cuts at: 0 328 475 1026 Size: 328 147 551 Fragments arranged by size: 551 328 147 SfcI C'TryA_G Cuts at: 0 697 1026 Size: 697 329 SmaI CCC'GGG Cuts at: 0 299 326 1026 Size: 299 27 700 Fragments arranged by size: 700 299 27 SmlI C'TyrA_G Cuts at: 0 33 166 1026 Size: 33 133 860 Fragments arranged by size: 860 133 33 SphI G_CATG'C Cuts at: 0 642 1026 Size: 642 384 StuI AGG'CCT Cuts at: 0 229 1026 Size: 229 797 StyI C'CwwG_G Cuts at: 0 48 735 1026 Size: 48 687 291 Fragments arranged by size: 687 291 48 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 734 766 914 946 1026 Size: 734 32 148 32 80 Fragments arranged by size: 734 148 80 32 32 XhoI C'TCGA_G Cuts at: 0 33 1026 Size: 33 993 XmaI C'CCGG_G Cuts at: 0 297 324 1026 Size: 297 27 702 Fragments arranged by size: 702 297 27 Enzymes that do cut: AlfI ApaI ApaLI AvaI BanI BanII BglI Bme1580I BmrI BpmI BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrFI BstAPI BtgI BtgZI BtsI EaeI EagI EciI Eco57MI EcoNI EcoO109I FspI FspAI HaeII Hin4I MspA1I NcoI NotI NspI PmlI PpuMI PspOMI PstI RsrII SacII SanDI SfcI SmaI SmlI SphI StuI StyI TstI XhoI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AloI AlwNI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I BsaI BsaBI BsgI BsiWI BsmI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EarI EcoICRI EcoRI EcoRV FalI FseI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI NaeI NarI NdeI NgoMIV NheI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TaqII TatI Tth111I XbaI XcmI XmnI ZraI