Restriction map for
pFN21AE2687
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI AlwI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbrPI
BbsI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BsiWI BspDI
BspEI BspHI BspMI BsrDI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I CelII ClaI CpoI
DraI DraIII Eam1105I EcoNI EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HpaI KpnI KspI MamI MfeI
MflI MluI MroI MunI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI ScaI
SfuI SgrAI SnaBI SpeI SphI
SspI SwaI TfiI XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 952 GTCTAC GT^[AC][GT]AC
AflIII 902 ACATGT A^C[GA][CT]GT
Alw44I 547 GTGCAC G^TGCAC
Aor51HI 179 AGCGCT AGC^GCT
ApaLI 547 GTGCAC G^TGCAC
AvaI 851 CCCGGG C^[CT]CG[GA]G
BcgI 704 GCACTGATATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 223 CTAG C^TAG
BsiEI 74 CGGCCG CG[GA][CT]^CG
BsmBI 829 CGTCTC CGTCTC(1/5)
BsmI 1443 GAATGC GAATGC(1/-1)
BsoBI 851 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1487 TGTACA T^GTACA
BspLU11I 902 ACATGT A^CATGT
BsrBI 146 GAGCGG CCGCTC(-3/-3)
BsrGI 1487 TGTACA T^GTACA
BsrI 1380 CCAGT ACTGG(1/-1)
BssSI 1345 CACGAG CACGAG(-5/-1)
BstAPI 390 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DpnII 1350 GATC ^GATC
DrdI 366 GACTCAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 74 CGGCCG C^GGCCG
EclXI 74 CGGCCG C^GGCCG
Eco47III 179 AGCGCT AGC^GCT
Eco52I 74 CGGCCG C^GGCCG
HincII 1125 GTCAAC GT[CT]^[GA]AC
HindII 1125 GTCAAC GT[CT]^[GA]AC
HindIII 1225 AAGCTT A^AGCTT
MaeI 223 CTAG C^TAG
MaeII 857 ACGT A^CGT
MboI 1350 GATC ^GATC
MseI 1241 TTAA T^TAA
NaeI 486 GCCGGC GCC^GGC
NdeII 1350 GATC ^GATC
NgoMIV 486 GCCGGC G^CCGGC
NheI 222 GCTAGC G^CTAGC
PflMI 1107 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1205 GGGACCT [GA]G^G[AT]CC[CT]
Sau3AI 1350 GATC ^GATC
SbfI 1507 CCTGCAGG CCTGCA^GG
SexAI 1208 ACCTGGT A^CC[AT]GGT
SmaI 851 CCCGGG CCC^GGG
Sse8387I 1507 CCTGCAGG CCTGCA^GG
SspBI 1487 TGTACA T^GTACA
StuI 308 AGGCCT AGG^CCT
TaqI 1352 TCGA T^CGA
Tru9I 1241 TTAA T^TAA
Tsp509I 1218 AATT ^AATT
TthHB8I 1352 TCGA T^CGA
Van91I 1107 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 851 CCCGGG C^CCGGG
XmaI 851 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AlwNI 391 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
967 CAGCCCCTG
AocI 277 CCTGAGG CC^T[ACGT]AGG
650 CCTCAGG
ApaI 665 GGGCCC GGGCC^C
840 GGGCCC
AspHI 286 GTGCTC G[AT]GC[AT]^C
547 GTGCAC
AspI 1119 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1207 GACCTGGTC
BalI 603 TGGCCA TGG^CCA
1152 TGGCCA
BbeI 34 GGCGCC GGCGC^C
98 GGCGCC
BseRI 648 CTCCTC GAGGAG(10/8)
735 CTCCTC
BsiHKAI 286 GTGCTC G[AT]GC[AT]^C
547 GTGCAC
BsrFI 486 GCCGGC [GA]^CCGG[CT]
1635 GCCGGT
Bsu36I 277 CCTGAGG CC^T[ACGT]AGG
650 CCTCAGG
Cfr10I 486 GCCGGC [GA]^CCGG[CT]
1635 GCCGGT
Eco81I 277 CCTGAGG CC^T[ACGT]AGG
650 CCTCAGG
EcoRV 709 GATATC GAT^ATC
1585 GATATC
HgaI 407 GCGTC GACGC(5/10)
828 GCGTC
KasI 34 GGCGCC G^GCGCC
98 GGCGCC
MluNI 603 TGGCCA TGG^CCA
1152 TGGCCA
MscI 603 TGGCCA TGG^CCA
1152 TGGCCA
MspA1I 241 CAGCTG C[AC]G^C[GT]G
370 CAGCTG
NarI 34 GGCGCC GG^CGCC
98 GGCGCC
NcoI 619 CCATGG C^CATGG
845 CCATGG
NspI 513 GCATGT [GA]CATG^[CT]
902 ACATGT
PflFI 1119 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1207 GACCTGGTC
PvuII 241 CAGCTG CAG^CTG
370 CAGCTG
SapI 288 GCTCTTC GCTCTTC(1/4)
1137 GCTCTTC
SfiI 273 GGCCCCTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1458 GGCCACAGGGGCC
SfoI 34 GGCGCC GGC^GCC
98 GGCGCC
Tth111I 1119 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1207 GACCTGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaII BanI BanII BbvCI BbvI
BcnI BglI BmyI BpmI BsaHI
BsaI BsaJI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboII MnlI
MslI MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PleI PstI
RsaI Sau96I ScrFI SfaNI SfcI
SmlI StyI TseI Tsp45I TspRI