Restriction map for
pFN21AE2687
(Circular) (Six-base) MAPSORT of: pFN21AE2687.seq Check: 9926 from: 1 to: 6442
pFN21AE2687 6442 bp
With 182 enzymes: SgfI *
May 22, 2017 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4252 278
Size: 53 83 186 936 2716 2468
Fragments arranged by size:
2716 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2958 3785 1937
Size: 1021 827 4594
Fragments arranged by size:
4594 1021 827
Acc65I G'GTAC_C
Cuts at: 3763 3763
Size: 6442
AclI AA'CG_TT
Cuts at: 1563 4569 4942 1563
Size: 3006 373 3063
Fragments arranged by size:
3063 3006 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2546 2822 2929 3130 3248 4505 5889 6157
Size: 914 276 107 201 118 1257 1384 268
Cuts at: 6157 1632
Size: 1917
Fragments arranged by size:
1917 1384 1257 914 276 268 201 118
107
AfeI AGC'GCT
Cuts at: 2186 2186
Size: 6442
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6245
AflIII A'CryG_T
Cuts at: 1203 2907 5341 6318 1203
Size: 1704 2434 977 1327
Fragments arranged by size:
2434 1704 1327 977
AgeI A'CCGG_T
Cuts at: 5273 5273
Size: 6442
AhdI GACnn_n'nnGTC
Cuts at: 5171 5171
Size: 6442
AleI CACnn'nnGTG
Cuts at: 1166 2303 3536 1166
Size: 1137 1233 4072
Fragments arranged by size:
4072 1233 1137
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6442
AlwNI CAG_nnn'CTG
Cuts at: 2401 2977 4185 5757 2401
Size: 576 1208 1572 3086
Fragments arranged by size:
3086 1572 1208 576
ApaI G_GGCC'C
Cuts at: 2674 2849 2674
Size: 175 6267
ApaLI G'TGCA_C
Cuts at: 2552 4499 5655 2552
Size: 1947 1156 3339
Fragments arranged by size:
3339 1947 1156
ApoI r'AATT_y
Cuts at: 1493 3751 3977 4129 6238 1493
Size: 2258 226 152 2109 1697
Fragments arranged by size:
2258 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4996 160
Size: 872 3964 1606
Fragments arranged by size:
3964 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
AvaI C'yCGr_G
Cuts at: 1870 1943 2856 3767 1870
Size: 73 913 911 4545
Fragments arranged by size:
4545 913 911 73
BamHI G'GATC_C
Cuts at: 3772 4139 3772
Size: 367 6075
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 2103 2488 2537 3523
Size: 325 217 627 252 64 385 49 986
Cuts at: 3523 3763 5218 618
Size: 240 1455 1842
Fragments arranged by size:
1842 1455 986 627 385 325 252 240
217 64 49
BanII G_rGCy'C
Cuts at: 1391 2654 2674 2849 2893 3144 3761 1391
Size: 1263 20 175 44 251 617 4072
Fragments arranged by size:
4072 1263 617 251 175 44 20
BbeI G_GCGC'C
Cuts at: 2043 2107 2043
Size: 64 6378
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5471
BbvCI CC'TCA_GC
Cuts at: 2244 2786 3302 3546 2244
Size: 542 516 244 5140
Fragments arranged by size:
5140 542 516 244
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2698 2732 4633 4667 1903
Size: 34 761 34 1901 34 3678
Fragments arranged by size:
3678 1901 761 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4334 5544 4334
Size: 1210 5232
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6442
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2285 2649 2805
Size: 107 122 71 631 605 613 364 156
Cuts at: 2805 3470 5053 136
Size: 665 1583 1525
Fragments arranged by size:
1583 1525 665 631 613 605 364 156
122 107 71
BglII A'GATC_T
Cuts at: 6437 6437
Size: 6442
BlpI GC'TnA_GC
Cuts at: 3859 3859
Size: 6442
Bme1580I G_kGCm'C
Cuts at: 1790 2547 2556 2674 2849 3078 4503 5659 1790
Size: 757 9 118 175 229 1425 1156 2573
Fragments arranged by size:
2573 1425 1156 757 229 175 118 9
BmgBI CAC'GTC
Cuts at: 1318 2863 1318
Size: 1545 4897
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3379 4173 5131
Size: 416 53 83 399 24 1962 794 958
Cuts at: 5131 442
Size: 1753
Fragments arranged by size:
1962 1753 958 794 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2231 1056
Size: 1175 5267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2180 2706 3065 3140 5102 1239
Size: 941 526 359 75 1962 2579
Fragments arranged by size:
2579 1962 941 526 359 75
Bpu10I CC'TnA_GC
Cuts at: 2244 2704 2786 3302 3546 2244
Size: 460 82 516 244 5140
Fragments arranged by size:
5140 516 460 244 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2411 2491 2750 3083 4567 5432 5730 2411
Size: 80 259 333 1484 865 298 3123
Fragments arranged by size:
3123 1484 865 333 298 259 80
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3041 3116 3223 5105 882
Size: 617 1542 75 107 1882 2219
Fragments arranged by size:
2219 1882 1542 617 107 75
BsaAI yAC'GTr
Cuts at: 493 1204 4233 493
Size: 711 3029 2702
Fragments arranged by size:
3029 2702 711
BsaBI GATnn'nnATC
Cuts at: 6436 6436
Size: 6442
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2040 2104 2412 4249
Size: 53 83 186 936 507 64 308 1837
Cuts at: 4249 4631 5266 275
Size: 382 635 1451
Fragments arranged by size:
1837 1451 936 635 507 382 308 186
83 64 53
BsaWI w'CCGG_w
Cuts at: 4875 5273 5547 5694 6105 4875
Size: 398 274 147 411 5212
Fragments arranged by size:
5212 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2917 2947 3113 3143 1178
Size: 30 165 30 1514 30 166 30 4477
Fragments arranged by size:
4477 1514 166 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2644 2731 1186
Size: 1458 87 4897
Fragments arranged by size:
4897 1458 87
BseYI C'CCAG_C
Cuts at: 2464 5645 6141 2464
Size: 3181 496 2765
Fragments arranged by size:
3181 2765 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2030 2273 3671 1163
Size: 573 294 243 1398 3934
Fragments arranged by size:
3934 1398 573 294 243
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2082 3892 4653 5681 1540
Size: 275 186 81 1810 761 1028 2301
Fragments arranged by size:
2301 1810 1028 761 275 186 81
BsiHKAI G_wGCw'C
Cuts at: 2295 2556 4503 4588 5659 2295
Size: 261 1947 85 1071 3078
Fragments arranged by size:
3078 1947 1071 261 85
BsmI GAATG_Cn'
Cuts at: 3454 3953 4046 3454
Size: 499 93 5850
Fragments arranged by size:
5850 499 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2840 2840
Size: 6442
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2295 2547 2556 2654 2674 2849 2893
Size: 399 505 252 9 98 20 175 44
Cuts at: 2893 3078 3144 3761 4503 4588 5659 1391
Size: 185 66 617 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 617 505 399 252 185
175 98 85 66 44 20 9
BspEI T'CCGG_A
Cuts at: 6105 6105
Size: 6442
BspHI T'CATG_A
Cuts at: 4331 6061 4331
Size: 1730 4712
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3797 844
Size: 2953 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2153 4329 1394
Size: 429 330 2176 3507
Fragments arranged by size:
3507 2176 429 330
BsrDI GCAATG_nn'
Cuts at: 65 1248 4937 5111 65
Size: 1183 3689 174 1396
Fragments arranged by size:
3689 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2491 3640 5086 5273 1700
Size: 110 681 1149 1446 187 2869
Fragments arranged by size:
2869 1446 1149 681 187 110
BsrGI T'GTAC_A
Cuts at: 96 3492 96
Size: 3396 3046
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6442
BssSI C'ACGA_G
Cuts at: 3350 4502 5514 3350
Size: 1152 1012 4278
Fragments arranged by size:
4278 1152 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2401 794
Size: 1607 4835
BstBI TT'CG_AA
Cuts at: 5246 5246
Size: 6442
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6442
BstYI r'GATC_y
Cuts at: 1972 3772 4139 4524 4541 5993 6079 6091 6437
Size: 1800 367 385 17 1452 86 12 346
Cuts at: 6437 1972
Size: 1977
Fragments arranged by size:
1977 1800 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2283 2656 2283
Size: 373 6069
BtgI C'CryG_G
Cuts at: 513 1065 2004 2082 2624 2841 2850 3534 513
Size: 552 939 78 542 217 9 684 3421
Fragments arranged by size:
3421 939 684 552 542 217 78 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5703
Fragments arranged by size:
5703 683 56
BtsI GCAGTG_nn'
Cuts at: 2835 3659 3958 4752 4772 2835
Size: 824 299 794 20 4505
Fragments arranged by size:
4505 824 794 299 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6442
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6407
DraI TTT'AAA
Cuts at: 3746 3746
Size: 6442
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2377 5449 809
Size: 507 1061 3072 1802
Fragments arranged by size:
3072 1802 1061 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2608 3024
Size: 54 1425 50 275 18 249 529 416
Cuts at: 3024 3157 3645 3889 4778 6407 8
Size: 133 488 244 889 1629 43
Fragments arranged by size:
1629 1425 889 529 488 416 275 249
244 133 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2079 3889 1537
Size: 275 267 1810 4090
Fragments arranged by size:
4090 1810 275 267
EarI CTCTTCn'nnn_
Cuts at: 1353 2300 2946 3149 3620 3672 4372 1353
Size: 947 646 203 471 52 700 3423
Fragments arranged by size:
3423 947 700 646 471 203 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2510 5029 5403 5549 387
Size: 1064 138 921 2519 374 146 1280
Fragments arranged by size:
2519 1280 1064 921 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 2546 2706 2822 2929 3065 3130
Size: 393 548 366 160 116 107 136 65
Cuts at: 3130 3140 3248 4505 5102 5889 6157 1239
Size: 10 108 1257 597 787 268 1524
Fragments arranged by size:
1524 1257 787 597 548 393 366 268
160 136 116 108 107 65 10
EcoO109I rG'GnC_Cy
Cuts at: 2398 2670 2671 2846 3211 2398
Size: 272 1 175 365 5629
Fragments arranged by size:
5629 365 272 175 1
EcoRI G'AATT_C
Cuts at: 3751 4129 3751
Size: 378 6064
EcoRV GAT'ATC
Cuts at: 2716 3592 2716
Size: 876 5566
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2452 2452
Size: 6442
FspI TGC'GCA
Cuts at: 4948 4948
Size: 6442
HaeII r_GCGC'y
Cuts at: 1678 2043 2107 2188 2225 3398 5589 1678
Size: 365 64 81 37 1173 2191 2531
Fragments arranged by size:
2531 2191 1173 365 81 64 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2198 2230 2338 2370 2712
Size: 32 135 32 626 32 108 32 342
Cuts at: 2712 2744 2940 2972 3113 3145 3203 3235 5083
Size: 32 196 32 141 32 58 32 1848
Cuts at: 5083 5115 5157 5189 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1848 626 342 196 141 135 108
58 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3132 3786 4032 669
Size: 988 281 1194 654 246 3079
Fragments arranged by size:
3079 1194 988 654 281 246
HindIII A'AGCT_T
Cuts at: 748 3230 748
Size: 2482 3960
HpaI GTT'AAC
Cuts at: 4032 4032
Size: 6442
KasI G'GCGC_C
Cuts at: 2039 2103 2039
Size: 64 6378
KpnI G_GTAC'C
Cuts at: 3767 3767
Size: 6442
MfeI C'AATT_G
Cuts at: 4041 4041
Size: 6442
MluI A'CGCG_T
Cuts at: 6318 6318
Size: 6442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5556 5740 1305
Size: 4251 184 2007
Fragments arranged by size:
4251 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2610 3159 10
Size: 54 1425 343 778 549 3293
Fragments arranged by size:
3293 1425 778 549 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2303 2559 3536 4400 4759 4918 518
Size: 648 1137 256 977 864 359 159 2042
Fragments arranged by size:
2042 1137 977 864 648 359 256 159
MspA1I CmG'CkG
Cuts at: 1606 2248 2377 3858 4535 5683 5928 1606
Size: 642 129 1481 677 1148 245 2120
Fragments arranged by size:
2120 1481 1148 677 642 245 129
NaeI GCC'GGC
Cuts at: 2493 2493
Size: 6442
NarI GG'CG_CC
Cuts at: 2040 2104 2040
Size: 64 6378
NcoI C'CATG_G
Cuts at: 513 1065 2004 2624 2850 513
Size: 552 939 620 226 4105
Fragments arranged by size:
4105 939 620 552 226
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6442
NgoMIV G'CCGG_C
Cuts at: 2491 2491
Size: 6442
NheI G'CTAG_C
Cuts at: 1052 2227 1052
Size: 1175 5267
NotI GC'GGCC_GC
Cuts at: 3889 3889
Size: 6442
NspI r_CATG'y
Cuts at: 1132 1229 2522 2911 3800 5345 1132
Size: 97 1293 389 889 1545 2229
Fragments arranged by size:
2229 1545 1293 889 389 97
PasI CC'CwG_GG
Cuts at: 1348 2645 2698 1348
Size: 1297 53 5092
Fragments arranged by size:
5092 1297 53
PciI A'CATG_T
Cuts at: 2907 5341 2907
Size: 2434 4008
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3118 1108
Size: 2010 4432
PmeI GTTT'AAAC
Cuts at: 3746 3746
Size: 6442
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2894 2926 4511 4543 6024 6056 2894
Size: 32 1585 32 1481 32 3280
Fragments arranged by size:
3280 1585 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3211 3211
Size: 6442
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6442
PsiI TTA'TAA
Cuts at: 4012 4012
Size: 6442
PspOMI G'GGCC_C
Cuts at: 2670 2845 2670
Size: 175 6267
PstI C_TGCA'G
Cuts at: 830 2535 2594 3348 3517 3612 3794 830
Size: 1705 59 754 169 95 182 3478
Fragments arranged by size:
3478 1705 754 182 169 95 59
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6442
PvuII CAG'CTG
Cuts at: 2248 2377 2248
Size: 129 6313
SalI G'TCGA_C
Cuts at: 1936 3784 1936
Size: 1848 4594
SapI GCTCTTCn'nnn_
Cuts at: 2300 3149 2300
Size: 849 5593
SbfI CC_TGCA'GG
Cuts at: 3517 3794 3517
Size: 277 6165
ScaI AGT'ACT
Cuts at: 4690 6402 4690
Size: 1712 4730
SexAI A'CCwGG_T
Cuts at: 3213 3213
Size: 6442
SfcI C'TryA_G
Cuts at: 826 1045 2531 2590 3170 3344 3513 3608 3790
Size: 219 1486 59 580 174 169 95 182
Cuts at: 3790 4925 5606 5797 826
Size: 1135 681 191 1471
Fragments arranged by size:
1486 1471 1135 681 580 219 191 182
174 169 95 59
SfiI GGCCn_nnn'nGGCC
Cuts at: 2285 3470 2285
Size: 1185 5257
SfoI GGC'GCC
Cuts at: 2041 2105 2041
Size: 64 6378
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6442
SmaI CCC'GGG
Cuts at: 1872 2858 3769 1872
Size: 986 911 4545
Fragments arranged by size:
4545 986 911
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2426 2470 2765 3062 4546 5447
Size: 197 926 483 44 295 297 1484 901
Cuts at: 5447 5709 820
Size: 262 1553
Fragments arranged by size:
1553 1484 926 901 483 297 295 262
197 44
SnaBI TAC'GTA
Cuts at: 493 4233 493
Size: 3740 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6442
SphI G_CATG'C
Cuts at: 1132 3800 1132
Size: 2668 3774
SspI AAT'ATT
Cuts at: 5 52 4366 5
Size: 47 4314 2081
Fragments arranged by size:
4314 2081 47
StuI AGG'CCT
Cuts at: 1517 2315 1517
Size: 798 5644
StyI C'CwwG_G
Cuts at: 513 1065 2004 2624 2850 3284 3881 513
Size: 552 939 620 226 434 597 3074
Fragments arranged by size:
3074 939 620 597 552 434 226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4469 4486 4639 4824 5269 325
Size: 1255 2889 17 153 185 445 1498
Fragments arranged by size:
2889 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3492 4688 6400
Size: 274 80 33 51 1443 1515 1196 1712
Cuts at: 6400 96
Size: 138
Fragments arranged by size:
1712 1515 1443 1196 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2326 2358 2548 2580 2917 2949 3070 3102 6402
Size: 32 190 32 337 32 121 32 3300
Cuts at: 6402 6434 2326
Size: 32 2334
Fragments arranged by size:
3300 2334 337 190 121 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3127 3215 1316
Size: 1811 88 4543
Fragments arranged by size:
4543 1811 88
XbaI T'CTAG_A
Cuts at: 3778 3778
Size: 6442
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6442
XmaI C'CCGG_G
Cuts at: 1870 2856 3767 1870
Size: 986 911 4545
Fragments arranged by size:
4545 986 911
XmnI GAAnn'nnTTC
Cuts at: 4571 4571
Size: 6442
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4250 276
Size: 53 83 186 936 2716 2468
Fragments arranged by size:
2716 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstXI BstZ17I DraIII EcoICRI EcoNI FseI FspAI NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI
SacII SanDI SrfI SwaI XcmI