(Linear) (Six-base) MAPSORT of: KIEE2687.seq Check: 9269 from: 1 to: 1737 KIEE2687.orf With 182 enzymes: SgfI * May 19, 2017 16:27 .. AccI GT'mk_AC Cuts at: 0 953 1737 Size: 953 784 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 541 817 924 1125 1243 1737 Size: 541 276 107 201 118 494 Fragments arranged by size: 541 494 276 201 118 107 AfeI AGC'GCT Cuts at: 0 181 1737 Size: 181 1556 AflIII A'CryG_T Cuts at: 0 902 1737 Size: 902 835 AleI CACnn'nnGTG Cuts at: 0 298 1531 1737 Size: 298 1233 206 Fragments arranged by size: 1233 298 206 AlwNI CAG_nnn'CTG Cuts at: 0 396 972 1737 Size: 396 576 765 Fragments arranged by size: 765 576 396 ApaI G_GGCC'C Cuts at: 0 669 844 1737 Size: 669 175 893 Fragments arranged by size: 893 669 175 ApaLI G'TGCA_C Cuts at: 0 547 1737 Size: 547 1190 AvaI C'yCGr_G Cuts at: 0 851 1737 Size: 851 886 BanI G'GyrC_C Cuts at: 0 34 98 483 532 1518 1737 Size: 34 64 385 49 986 219 Fragments arranged by size: 986 385 219 64 49 34 BanII G_rGCy'C Cuts at: 0 649 669 844 888 1139 1737 Size: 649 20 175 44 251 598 Fragments arranged by size: 649 598 251 175 44 20 BbeI G_GCGC'C Cuts at: 0 38 102 1737 Size: 38 64 1635 Fragments arranged by size: 1635 64 38 BbvCI CC'TCA_GC Cuts at: 0 239 781 1297 1541 1737 Size: 239 542 516 244 196 Fragments arranged by size: 542 516 244 239 196 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 693 727 1737 Size: 693 34 1010 Fragments arranged by size: 1010 693 34 BglI GCCn_nnn'nGGC Cuts at: 0 280 644 800 1465 1737 Size: 280 364 156 665 272 Fragments arranged by size: 665 364 280 272 156 Bme1580I G_kGCm'C Cuts at: 0 542 551 669 844 1073 1737 Size: 542 9 118 175 229 664 Fragments arranged by size: 664 542 229 175 118 9 BmgBI CAC'GTC Cuts at: 0 858 1737 Size: 858 879 BmrI ACTGGGnnnn_n' Cuts at: 0 1374 1737 Size: 1374 363 BmtI G_CTAG'C Cuts at: 0 226 1737 Size: 226 1511 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 175 701 1060 1135 1737 Size: 175 526 359 75 602 Fragments arranged by size: 602 526 359 175 75 Bpu10I CC'TnA_GC Cuts at: 0 239 699 781 1297 1541 1737 Size: 239 460 82 516 244 196 Fragments arranged by size: 516 460 244 239 196 82 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 406 486 745 1078 1737 Size: 406 80 259 333 659 Fragments arranged by size: 659 406 333 259 80 BsaI GGTCTCn'nnnn_ Cuts at: 0 1036 1111 1218 1737 Size: 1036 75 107 519 Fragments arranged by size: 1036 519 107 75 BsaHI Gr'CG_yC Cuts at: 0 35 99 407 1737 Size: 35 64 308 1330 Fragments arranged by size: 1330 308 64 35 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 912 942 1108 1138 1737 Size: 912 30 166 30 599 Fragments arranged by size: 912 599 166 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 639 726 1737 Size: 639 87 1011 Fragments arranged by size: 1011 639 87 BseYI C'CCAG_C Cuts at: 0 459 1737 Size: 459 1278 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 25 268 1666 1737 Size: 25 243 1398 71 Fragments arranged by size: 1398 243 71 25 BsiEI CG_ry'CG Cuts at: 0 77 1737 Size: 77 1660 BsiHKAI G_wGCw'C Cuts at: 0 290 551 1737 Size: 290 261 1186 Fragments arranged by size: 1186 290 261 BsmI GAATG_Cn' Cuts at: 0 1449 1737 Size: 1449 288 BsmBI CGTCTCn'nnnn_ Cuts at: 0 835 1737 Size: 835 902 Bsp1286I G_dGCh'C Cuts at: 0 290 542 551 649 669 844 888 1073 Size: 290 252 9 98 20 175 44 185 Cuts at: 1073 1139 1737 Size: 66 598 Fragments arranged by size: 598 290 252 185 175 98 66 44 20 9 BsrBI CCG'CTC Cuts at: 0 148 1737 Size: 148 1589 BsrFI r'CCGG_y Cuts at: 0 486 1635 1737 Size: 486 1149 102 Fragments arranged by size: 1149 486 102 BsrGI T'GTAC_A Cuts at: 0 1487 1737 Size: 1487 250 BssSI C'ACGA_G Cuts at: 0 1345 1737 Size: 1345 392 BstAPI GCAn_nnn'nTGC Cuts at: 0 396 1737 Size: 396 1341 Bsu36I CC'TnA_GG Cuts at: 0 278 651 1737 Size: 278 373 1086 Fragments arranged by size: 1086 373 278 BtgI C'CryG_G Cuts at: 0 77 619 836 845 1529 1737 Size: 77 542 217 9 684 208 Fragments arranged by size: 684 542 217 208 77 9 BtsI GCAGTG_nn' Cuts at: 0 830 1654 1737 Size: 830 824 83 Fragments arranged by size: 830 824 83 DrdI GACnn_nn'nnGTC Cuts at: 0 372 1737 Size: 372 1365 EaeI y'GGCC_r Cuts at: 0 74 603 1019 1152 1640 1737 Size: 74 529 416 133 488 97 Fragments arranged by size: 529 488 416 133 97 74 EagI C'GGCC_G Cuts at: 0 74 1737 Size: 74 1663 EarI CTCTTCn'nnn_ Cuts at: 0 295 941 1144 1615 1667 1737 Size: 295 646 203 471 52 70 Fragments arranged by size: 646 471 295 203 70 52 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 505 1737 Size: 505 1232 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 175 541 701 817 924 1060 1125 1135 Size: 175 366 160 116 107 136 65 10 Cuts at: 1135 1243 1737 Size: 108 494 Fragments arranged by size: 494 366 175 160 136 116 108 107 65 10 EcoO109I rG'GnC_Cy Cuts at: 0 393 665 666 841 1206 1737 Size: 393 272 1 175 365 531 Fragments arranged by size: 531 393 365 272 175 1 EcoRV GAT'ATC Cuts at: 0 711 1587 1737 Size: 711 876 150 Fragments arranged by size: 876 711 150 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 447 1737 Size: 447 1290 HaeII r_GCGC'y Cuts at: 0 38 102 183 220 1393 1737 Size: 38 64 81 37 1173 344 Fragments arranged by size: 1173 344 81 64 38 37 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 193 225 333 365 707 739 935 967 Size: 193 32 108 32 342 32 196 32 Cuts at: 967 1108 1140 1198 1230 1737 Size: 141 32 58 32 507 Fragments arranged by size: 507 342 196 193 141 108 58 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1127 1737 Size: 1127 610 HindIII A'AGCT_T Cuts at: 0 1225 1737 Size: 1225 512 KasI G'GCGC_C Cuts at: 0 34 98 1737 Size: 34 64 1639 Fragments arranged by size: 1639 64 34 MscI TGG'CCA Cuts at: 0 605 1154 1737 Size: 605 549 583 Fragments arranged by size: 605 583 549 MslI CAynn'nnrTG Cuts at: 0 298 554 1531 1737 Size: 298 256 977 206 Fragments arranged by size: 977 298 256 206 MspA1I CmG'CkG Cuts at: 0 243 372 1737 Size: 243 129 1365 Fragments arranged by size: 1365 243 129 NaeI GCC'GGC Cuts at: 0 488 1737 Size: 488 1249 NarI GG'CG_CC Cuts at: 0 35 99 1737 Size: 35 64 1638 Fragments arranged by size: 1638 64 35 NcoI C'CATG_G Cuts at: 0 619 845 1737 Size: 619 226 892 Fragments arranged by size: 892 619 226 NgoMIV G'CCGG_C Cuts at: 0 486 1737 Size: 486 1251 NheI G'CTAG_C Cuts at: 0 222 1737 Size: 222 1515 NspI r_CATG'y Cuts at: 0 517 906 1737 Size: 517 389 831 Fragments arranged by size: 831 517 389 PasI CC'CwG_GG Cuts at: 0 640 693 1737 Size: 640 53 1044 Fragments arranged by size: 1044 640 53 PciI A'CATG_T Cuts at: 0 902 1737 Size: 902 835 PflMI CCAn_nnn'nTGG Cuts at: 0 1113 1737 Size: 1113 624 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 889 921 1737 Size: 889 32 816 Fragments arranged by size: 889 816 32 PpuMI rG'GwC_Cy Cuts at: 0 1206 1737 Size: 1206 531 PspOMI G'GGCC_C Cuts at: 0 665 840 1737 Size: 665 175 897 Fragments arranged by size: 897 665 175 PstI C_TGCA'G Cuts at: 0 530 589 1343 1512 1607 1737 Size: 530 59 754 169 95 130 Fragments arranged by size: 754 530 169 130 95 59 PvuII CAG'CTG Cuts at: 0 243 372 1737 Size: 243 129 1365 Fragments arranged by size: 1365 243 129 SapI GCTCTTCn'nnn_ Cuts at: 0 295 1144 1737 Size: 295 849 593 Fragments arranged by size: 849 593 295 SbfI CC_TGCA'GG Cuts at: 0 1512 1737 Size: 1512 225 SexAI A'CCwGG_T Cuts at: 0 1208 1737 Size: 1208 529 SfcI C'TryA_G Cuts at: 0 526 585 1165 1339 1508 1603 1737 Size: 526 59 580 174 169 95 134 Fragments arranged by size: 580 526 174 169 134 95 59 SfiI GGCCn_nnn'nGGCC Cuts at: 0 280 1465 1737 Size: 280 1185 272 Fragments arranged by size: 1185 280 272 SfoI GGC'GCC Cuts at: 0 36 100 1737 Size: 36 64 1637 Fragments arranged by size: 1637 64 36 SmaI CCC'GGG Cuts at: 0 853 1737 Size: 853 884 SmlI C'TyrA_G Cuts at: 0 421 465 760 1057 1737 Size: 421 44 295 297 680 Fragments arranged by size: 680 421 297 295 44 StuI AGG'CCT Cuts at: 0 310 1737 Size: 310 1427 StyI C'CwwG_G Cuts at: 0 619 845 1279 1737 Size: 619 226 434 458 Fragments arranged by size: 619 458 434 226 TatI w'GTAC_w Cuts at: 0 1487 1737 Size: 1487 250 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 321 353 543 575 912 944 1065 1097 Size: 321 32 190 32 337 32 121 32 Cuts at: 1097 1737 Size: 640 Fragments arranged by size: 640 337 321 190 121 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1122 1210 1737 Size: 1122 88 527 Fragments arranged by size: 1122 527 88 XmaI C'CCGG_G Cuts at: 0 851 1737 Size: 851 886 Enzymes that do cut: AccI AcuI AfeI AflIII AleI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbvCI BcgI BglI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BsrBI BsrFI BsrGI BssSI BstAPI Bsu36I BtgI BtsI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI PciI PflMI PpiI PpuMI PspOMI PstI PvuII SapI SbfI SexAI SfcI SfiI SfoI SmaI SmlI StuI StyI TatI TstI Tth111I XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AhdI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BciVI BclI BfrBI BglII BlpI BplI BsaAI BsaBI BsaWI BsiWI BspEI BspHI BspMI BsrDI BssHII BstBI BstEII BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII EcoICRI EcoNI EcoRI FseI FspI FspAI HpaI KpnI MfeI MluI MmeI NdeI NotI NruI NsiI PacI PfoI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI ScaI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TaqII XbaI XcmI XhoI XmnI ZraI