Restriction map for
pFN21AE2098
##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AgeI AhdI Alw44I AocI
ApaLI ApoI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbvCI BciVI BglII BlnI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BsrDI BsrGI BssSI Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FseI FspI HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 22 CGCG CG^CG
AfaI 1923 GTAC GT^AC
AflII 1012 CTTAAG C^TTAAG
Aor51HI 2270 AGCGCT AGC^GCT
AscI 20 GGCGCGCC GG^CGCGCC
BbrPI 1619 CACGTG CAC^GTG
BcgI 633 GCAGTCACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 450 TGATCA T^GATCA
BfrI 1012 CTTAAG C^TTAAG
BsaI 565 GAGACC GGTCTC(1/5)
BsmAI 565 GAGAC GTCTC(1/5)
BspLU11I 364 ACATGT A^CATGT
BsrBI 186 GAGCGG CCGCTC(-3/-3)
BsrFI 911 GCCGGC [GA]^CCGG[CT]
BssHII 21 GCGCGC G^CGCGC
BstAPI 645 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 22 CGCG CG^CG
BstYI 1329 AGATCC [GA]^GATC[CT]
Cfr10I 911 GCCGGC [GA]^CCGG[CT]
Eco47III 2270 AGCGCT AGC^GCT
FbaI 450 TGATCA T^GATCA
HgaI 441 GCGTC GACGC(5/10)
HincII 1669 GTTGAC GT[CT]^[GA]AC
HindII 1669 GTTGAC GT[CT]^[GA]AC
HindIII 1361 AAGCTT A^AGCTT
MflI 1329 AGATCC [GA]^GATC[CT]
MseI 1013 TTAA T^TAA
MvnI 22 CGCG CG^CG
NaeI 911 GCCGGC GCC^GGC
NdeI 169 CATATG CA^TATG
NgoMIV 911 GCCGGC G^CCGGC
NheI 1135 GCTAGC G^CTAGC
PflMI 766 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1619 CACGTG CAC^GTG
PmlI 1619 CACGTG CAC^GTG
PshAI 436 GACAGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 1923 GTAC GT^AC
SapI 99 GAAGAGC GCTCTTC(1/4)
SmaI 80 CCCGGG CCC^GGG
TaqI 140 TCGA T^CGA
Tru9I 1013 TTAA T^TAA
Tsp509I 2066 AATT ^AATT
TthHB8I 140 TCGA T^CGA
Van91I 766 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1329 AGATCC [GA]^GATC[CT]
XmaCI 80 CCCGGG C^CCGGG
XmaI 80 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflIII 364 ACATGT A^C[GA][CT]GT
1618 ACACGT
AlwI 1330 GATCC GGATC(4/5)
1324 GGATC
BalI 572 TGGCCA TGG^CCA
905 TGGCCA
BglI 898 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2235 GCCAGATGGGC
BsaAI 410 TACGTG [CT]AC^GT[GA]
1619 CACGTG
BsmI 1710 GAATGC GAATGC(1/-1)
2145 GAATGC
BstEII 1067 GGTCACC G^GT[ACGT]ACC
2293 GGTCACC
BstPI 1067 GGTCACC G^GT[ACGT]ACC
2293 GGTCACC
EaeI 572 TGGCCA [CT]^GGCC[GA]
905 TGGCCA
EcoO65I 1067 GGTCACC G^GT[ACGT]ACC
2293 GGTCACC
MluNI 572 TGGCCA TGG^CCA
905 TGGCCA
MscI 572 TGGCCA TGG^CCA
905 TGGCCA
NcoI 65 CCATGG C^CATGG
669 CCATGG
NspI 364 ACATGT [GA]CATG^[CT]
512 ACATGC
PpuMI 838 GGGTCCC [GA]G^G[AT]CC[CT]
1652 GGGTCCT
SacI 102 GAGCTC GAGCT^C
1452 GAGCTC
XcmI 402 CCATGTTCTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1044 CCATGAGGCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcyI AluI AlwNI ApaI
AspHI AvaI AvaII BanI BanII
BbeI BbsI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MwoI NarI
NciI NdeII NlaIII NlaIV PleI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI StuI
StyI TfiI TseI Tsp45I TspRI