(Circular) (Six-base) MAPSORT of: pFN21AE2098.seq Check: 9020 from: 1 to: 7141 pFN21AE2098 7141 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2619 2965 3459 4311 2619 Size: 346 494 852 5449 Fragments arranged by size: 5449 852 494 346 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4951 278 Size: 53 83 186 936 3415 2468 Fragments arranged by size: 3415 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4484 1937 Size: 2547 4594 Acc65I G'GTAC_C Cuts at: 4462 4462 Size: 7141 AclI AA'CG_TT Cuts at: 1563 5268 5641 1563 Size: 3705 373 3063 Fragments arranged by size: 3705 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3255 3854 4136 5204 6588 6856 1632 Size: 1623 599 282 1068 1384 268 1917 Fragments arranged by size: 1917 1623 1384 1068 599 282 268 AfeI AGC'GCT Cuts at: 4277 4277 Size: 7141 AflII C'TTAA_G Cuts at: 820 1017 3017 820 Size: 197 2000 4944 Fragments arranged by size: 4944 2000 197 AflIII A'CryG_T Cuts at: 1203 2369 3623 6040 7017 1203 Size: 1166 1254 2417 977 1327 Fragments arranged by size: 2417 1327 1254 1166 977 AgeI A'CCGG_T Cuts at: 5972 5972 Size: 7141 AhdI GACnn_n'nnGTC Cuts at: 5870 5870 Size: 7141 AleI CACnn'nnGTG Cuts at: 1166 3820 1166 Size: 2654 4487 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7141 AlwNI CAG_nnn'CTG Cuts at: 2777 3233 3859 4012 4884 6456 2777 Size: 456 626 153 872 1572 3462 Fragments arranged by size: 3462 1572 872 626 456 153 ApaI G_GGCC'C Cuts at: 2123 2206 2827 3544 3726 2123 Size: 83 621 717 182 5538 Fragments arranged by size: 5538 717 621 182 83 ApaLI G'TGCA_C Cuts at: 5198 6354 5198 Size: 1156 5985 ApoI r'AATT_y Cuts at: 1493 4450 4676 4828 6937 1493 Size: 2957 226 152 2109 1697 Fragments arranged by size: 2957 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 2026 2026 Size: 7141 AseI AT'TA_AT Cuts at: 160 1032 5695 160 Size: 872 4663 1606 Fragments arranged by size: 4663 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7141 AvaI C'yCGr_G Cuts at: 1870 1943 2015 2085 2116 2814 2832 2964 3841 Size: 73 72 70 31 698 18 132 877 Cuts at: 3841 4466 1870 Size: 625 4545 Fragments arranged by size: 4545 877 698 625 132 73 72 70 31 18 BamHI G'GATC_C Cuts at: 4471 4838 4471 Size: 367 6774 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 2095 2313 3062 3133 Size: 325 217 627 224 84 218 749 71 Cuts at: 3133 4462 5917 618 Size: 1329 1455 1842 Fragments arranged by size: 1842 1455 1329 749 627 325 224 218 217 84 71 BanII G_rGCy'C Cuts at: 1391 2111 2123 2206 2553 2827 2861 3461 3509 Size: 720 12 83 347 274 34 600 48 Cuts at: 3509 3544 3726 3805 4460 1391 Size: 35 182 79 655 4072 Fragments arranged by size: 4072 720 655 600 347 274 182 83 79 48 35 34 12 BbeI G_GCGC'C Cuts at: 2015 2099 2317 2015 Size: 84 218 6839 Fragments arranged by size: 6839 218 84 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2345 2427 3243 928 Size: 971 446 82 816 4826 Fragments arranged by size: 4826 971 816 446 82 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2627 2661 5332 5366 1903 Size: 34 690 34 2671 34 3678 Fragments arranged by size: 3678 2671 690 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5033 6243 5033 Size: 1210 5931 BclI T'GATC_A Cuts at: 1548 2455 1548 Size: 907 6234 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2909 4246 Size: 107 122 71 631 605 337 900 1337 Cuts at: 4246 5752 136 Size: 1506 1525 Fragments arranged by size: 1525 1506 1337 900 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 7136 7136 Size: 7141 BlpI GC'TnA_GC Cuts at: 2547 2634 2886 3145 3455 4558 2547 Size: 87 252 259 310 1103 5130 Fragments arranged by size: 5130 1103 310 259 252 87 Bme1580I G_kGCm'C Cuts at: 1790 2123 2206 2827 3136 3544 3577 3726 5202 Size: 333 83 621 309 408 33 149 1476 Cuts at: 5202 6358 1790 Size: 1156 2573 Fragments arranged by size: 2573 1476 1156 621 408 333 309 149 83 33 BmgBI CAC'GTC Cuts at: 1318 2082 2864 1318 Size: 764 782 5595 Fragments arranged by size: 5595 782 764 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4872 5830 442 Size: 416 53 83 399 24 3455 958 1753 Fragments arranged by size: 3455 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3144 1056 Size: 2088 5053 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 2588 3069 3494 3635 3791 4274 4292 Size: 968 381 481 425 141 156 483 18 Cuts at: 4292 5801 1239 Size: 1509 2579 Fragments arranged by size: 2579 1509 968 483 481 425 381 156 141 18 Bpu10I CC'TnA_GC Cuts at: 3159 3159 Size: 7141 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2267 2582 5266 6131 6429 2267 Size: 315 2684 865 298 2979 Fragments arranged by size: 2979 2684 865 315 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2564 5804 882 Size: 617 1065 3240 2219 Fragments arranged by size: 3240 2219 1065 617 BsaAI yAC'GTr Cuts at: 493 1204 2417 3626 4932 493 Size: 711 1213 1209 1306 2702 Fragments arranged by size: 2702 1306 1213 1209 711 BsaBI GATnn'nnATC Cuts at: 7135 7135 Size: 7141 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 2096 2314 2446 Size: 53 83 186 936 479 84 218 132 Cuts at: 2446 4948 5330 5965 275 Size: 2502 382 635 1451 Fragments arranged by size: 2502 1451 936 635 479 382 218 186 132 84 83 53 BsaWI w'CCGG_w Cuts at: 5574 5972 6246 6393 6804 5574 Size: 398 274 147 411 5911 Fragments arranged by size: 5911 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2066 2096 1178 Size: 30 165 30 663 30 6223 Fragments arranged by size: 6223 663 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3088 3764 4189 1186 Size: 1902 676 425 4138 Fragments arranged by size: 4138 1902 676 425 BseYI C'CCAG_C Cuts at: 3037 3338 3804 4063 4278 6344 6840 3037 Size: 301 466 259 215 2066 496 3338 Fragments arranged by size: 3338 2066 496 466 301 259 215 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2462 2573 2937 3014 3246 1163 Size: 573 726 111 364 77 232 5058 Fragments arranged by size: 5058 726 573 364 232 111 77 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4591 5352 6380 1540 Size: 275 186 2590 761 1028 2301 Fragments arranged by size: 2590 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2111 2984 3205 3461 3643 3827 3996 5202 5287 Size: 873 221 256 182 184 169 1206 85 Cuts at: 5287 6358 2111 Size: 1071 2894 Fragments arranged by size: 2894 1206 1071 873 256 221 184 182 169 85 BsmI GAATG_Cn' Cuts at: 3721 4156 4652 4745 3721 Size: 435 496 93 6117 Fragments arranged by size: 6117 496 435 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2111 2123 2206 2553 2827 2861 2984 Size: 399 321 12 83 347 274 34 123 Cuts at: 2984 3136 3205 3461 3509 3544 3577 3643 3726 Size: 152 69 256 48 35 33 66 83 Cuts at: 3726 3805 3827 3996 4460 5202 5287 6358 1391 Size: 79 22 169 464 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 464 399 347 321 274 256 169 152 123 85 83 83 79 69 66 48 35 34 33 22 12 BspEI T'CCGG_A Cuts at: 6804 6804 Size: 7141 BspHI T'CATG_A Cuts at: 5030 6760 5030 Size: 1730 5411 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2619 2965 3459 4311 4385 4496 844 Size: 1775 346 494 852 74 111 3489 Fragments arranged by size: 3489 1775 852 494 346 111 74 BsrBI CCG'CTC Cuts at: 1394 1823 2193 5028 1394 Size: 429 370 2835 3507 Fragments arranged by size: 3507 2835 429 370 BsrDI GCAATG_nn' Cuts at: 65 1248 5636 5810 65 Size: 1183 4388 174 1396 Fragments arranged by size: 4388 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2916 5785 5972 1700 Size: 110 1106 2869 187 2869 Fragments arranged by size: 2869 2869 1106 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7141 BssHII G'CGCG_C Cuts at: 1925 2026 1925 Size: 101 7040 BssSI C'ACGA_G Cuts at: 5201 6213 5201 Size: 1012 6129 BstAPI GCAn_nnn'nTGC Cuts at: 794 2656 794 Size: 1862 5279 BstBI TT'CG_AA Cuts at: 5945 5945 Size: 7141 BstEII G'GTnAC_C Cuts at: 1184 3072 4298 1184 Size: 1888 1226 4027 Fragments arranged by size: 4027 1888 1226 BstXI CCAn_nnnn'nTGG Cuts at: 2389 3346 3899 4270 2389 Size: 957 553 371 5260 Fragments arranged by size: 5260 957 553 371 BstYI r'GATC_y Cuts at: 1972 3334 4471 4838 5223 5240 6692 6778 6790 Size: 1362 1137 367 385 17 1452 86 12 Cuts at: 6790 7136 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1362 1137 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2070 2674 3794 4285 4437 513 Size: 552 939 66 604 1120 491 152 3217 Fragments arranged by size: 3217 1120 939 604 552 491 152 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2905 3759 487 Size: 683 56 1679 854 3869 Fragments arranged by size: 3869 1679 854 683 56 BtsI GCAGTG_nn' Cuts at: 3602 3641 3815 4657 5451 5471 3602 Size: 39 174 842 794 20 5272 Fragments arranged by size: 5272 842 794 174 39 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7141 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7106 DraI TTT'AAA Cuts at: 4445 4445 Size: 7141 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6148 809 Size: 507 4832 1802 Fragments arranged by size: 4832 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2577 2910 4588 Size: 54 1425 50 275 18 747 333 1678 Cuts at: 4588 5477 7106 8 Size: 889 1629 43 Fragments arranged by size: 1678 1629 1425 889 747 333 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4588 1537 Size: 275 2776 4090 Fragments arranged by size: 4090 2776 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2099 2478 3112 3282 4316 5071 1353 Size: 746 379 634 170 1034 755 3423 Fragments arranged by size: 3423 1034 755 746 634 379 170 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2594 3021 3509 5728 6102 6248 Size: 1064 138 1005 427 488 2219 374 146 Cuts at: 6248 387 Size: 1280 Fragments arranged by size: 2219 1280 1064 1005 488 427 374 146 138 EcoICRI GAG'CTC Cuts at: 2109 3459 2109 Size: 1350 5791 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2588 3069 3255 3494 3635 3791 Size: 393 575 381 481 186 239 141 156 Cuts at: 3791 3854 4136 4274 4292 5204 5801 6588 6856 Size: 63 282 138 18 912 597 787 268 Cuts at: 6856 1239 Size: 1524 Fragments arranged by size: 1524 912 787 597 575 481 393 381 282 268 239 186 156 141 138 63 18 EcoO109I rG'GnC_Cy Cuts at: 2120 2203 2774 2824 2844 3218 3541 3658 3722 Size: 83 571 50 20 374 323 117 64 Cuts at: 3722 3723 4120 2120 Size: 1 397 5141 Fragments arranged by size: 5141 571 397 374 323 117 83 64 50 20 1 EcoRI G'AATT_C Cuts at: 4450 4828 4450 Size: 378 6763 FspI TGC'GCA Cuts at: 5647 5647 Size: 7141 HaeII r_GCGC'y Cuts at: 1678 2015 2099 2172 2317 4063 4279 6288 1678 Size: 337 84 73 145 1746 216 2009 2531 Fragments arranged by size: 2531 2009 1746 337 216 145 84 73 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2066 2098 2828 2860 3552 Size: 32 135 32 494 32 730 32 692 Cuts at: 3552 3581 3584 3613 4166 4178 4181 4198 4210 Size: 29 3 29 553 12 3 17 12 Cuts at: 4210 4213 5782 5814 5856 5888 1373 Size: 3 1569 32 42 32 2626 Fragments arranged by size: 2626 1569 730 692 553 494 135 42 32 32 32 32 32 32 29 29 17 12 12 3 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 3676 4485 4731 669 Size: 988 281 1738 809 246 3079 Fragments arranged by size: 3079 1738 988 809 281 246 HindIII A'AGCT_T Cuts at: 748 3366 748 Size: 2618 4523 HpaI GTT'AAC Cuts at: 4731 4731 Size: 7141 KasI G'GCGC_C Cuts at: 2011 2095 2313 2011 Size: 84 218 6839 Fragments arranged by size: 6839 218 84 KpnI G_GTAC'C Cuts at: 4466 4466 Size: 7141 MfeI C'AATT_G Cuts at: 4740 4740 Size: 7141 MluI A'CGCG_T Cuts at: 7017 7017 Size: 7141 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2521 3759 4251 6255 6439 1305 Size: 1216 1238 492 2004 184 2007 Fragments arranged by size: 2007 2004 1238 1216 492 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2579 2912 10 Size: 54 1425 343 747 333 4239 Fragments arranged by size: 4239 1425 747 343 333 54 MslI CAynn'nnrTG Cuts at: 518 1166 2429 3585 3820 5099 5458 5617 518 Size: 648 1263 1156 235 1279 359 159 2042 Fragments arranged by size: 2042 1279 1263 1156 648 359 235 159 MspA1I CmG'CkG Cuts at: 1606 2461 2653 3342 3691 3859 4213 4557 5234 Size: 855 192 689 349 168 354 344 677 Cuts at: 5234 6382 6627 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 855 689 677 354 349 344 245 192 168 NaeI GCC'GGC Cuts at: 2918 2918 Size: 7141 NarI GG'CG_CC Cuts at: 2012 2096 2314 2012 Size: 84 218 6839 Fragments arranged by size: 6839 218 84 NcoI C'CATG_G Cuts at: 513 1065 2004 2070 2674 4437 513 Size: 552 939 66 604 1763 3217 Fragments arranged by size: 3217 1763 939 604 552 66 NdeI CA'TA_TG Cuts at: 387 2175 387 Size: 1788 5353 NgoMIV G'CCGG_C Cuts at: 2916 2916 Size: 7141 NheI G'CTAG_C Cuts at: 1052 3140 1052 Size: 2088 5053 NotI GC'GGCC_GC Cuts at: 4588 4588 Size: 7141 NspI r_CATG'y Cuts at: 1132 1229 2373 2521 4499 6044 1132 Size: 97 1144 148 1978 1545 2229 Fragments arranged by size: 2229 1978 1545 1144 148 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7141 PciI A'CATG_T Cuts at: 2369 6040 2369 Size: 3671 3470 PflMI CCAn_nnn'nTGG Cuts at: 1108 2777 1108 Size: 1669 5472 PfoI T'CCnGG_A Cuts at: 3612 3612 Size: 7141 PmeI GTTT'AAAC Cuts at: 4445 4445 Size: 7141 PmlI CAC'GTG Cuts at: 3626 3626 Size: 7141 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5210 5242 6723 6755 5210 Size: 32 1481 32 5596 Fragments arranged by size: 5596 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2844 3658 2844 Size: 814 6327 PshAI GACnn'nnGTC Cuts at: 1613 2445 1613 Size: 832 6309 PsiI TTA'TAA Cuts at: 4711 4711 Size: 7141 PspOMI G'GGCC_C Cuts at: 2119 2202 2823 3540 3722 2119 Size: 83 621 717 182 5538 Fragments arranged by size: 5538 717 621 182 83 PstI C_TGCA'G Cuts at: 830 2526 2902 3190 3855 4493 830 Size: 1696 376 288 665 638 3478 Fragments arranged by size: 3478 1696 665 638 376 288 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7141 PvuII CAG'CTG Cuts at: 2653 3342 3859 4213 2653 Size: 689 517 354 5581 Fragments arranged by size: 5581 689 517 354 SacI G_AGCT'C Cuts at: 2111 3461 2111 Size: 1350 5791 SalI G'TCGA_C Cuts at: 1936 4483 1936 Size: 2547 4594 SanDI GG'GwC_CC Cuts at: 2844 2844 Size: 7141 SapI GCTCTTCn'nnn_ Cuts at: 2099 2099 Size: 7141 SbfI CC_TGCA'GG Cuts at: 4493 4493 Size: 7141 ScaI AGT'ACT Cuts at: 5389 7101 5389 Size: 1712 5429 SfcI C'TryA_G Cuts at: 826 1045 2522 2898 3186 3851 4004 4489 5624 Size: 219 1477 376 288 665 153 485 1135 Cuts at: 5624 6305 6496 826 Size: 681 191 1471 Fragments arranged by size: 1477 1471 1135 681 665 485 376 288 219 191 153 SfoI GGC'GCC Cuts at: 2013 2097 2315 2013 Size: 84 218 6839 Fragments arranged by size: 6839 218 84 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7141 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7141 SmaI CCC'GGG Cuts at: 1872 2087 4468 1872 Size: 215 2381 4545 Fragments arranged by size: 4545 2381 215 SmlI C'TyrA_G Cuts at: 820 1017 1943 2282 2597 3017 5245 6146 6408 Size: 197 926 339 315 420 2228 901 262 Cuts at: 6408 820 Size: 1553 Fragments arranged by size: 2228 1553 926 901 420 339 315 262 197 SnaBI TAC'GTA Cuts at: 493 4932 493 Size: 4439 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7141 SphI G_CATG'C Cuts at: 1132 4499 1132 Size: 3367 3774 SspI AAT'ATT Cuts at: 5 52 5065 5 Size: 47 5013 2081 Fragments arranged by size: 5013 2081 47 StuI AGG'CCT Cuts at: 1517 2021 2292 2347 2396 2810 3839 4415 1517 Size: 504 271 55 49 414 1029 576 4243 Fragments arranged by size: 4243 1029 576 504 414 271 55 49 StyI C'CwwG_G Cuts at: 513 1065 2004 2070 2674 3396 4167 4437 4580 Size: 552 939 66 604 722 771 270 143 Cuts at: 4580 513 Size: 3074 Fragments arranged by size: 3074 939 771 722 604 552 270 143 66 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3454 3696 5168 5185 5338 5523 5968 Size: 1255 1874 242 1472 17 153 185 445 Cuts at: 5968 325 Size: 1498 Fragments arranged by size: 1874 1498 1472 1255 445 242 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3927 5387 7099 Size: 274 80 33 51 1443 1950 1460 1712 Cuts at: 7099 96 Size: 138 Fragments arranged by size: 1950 1712 1460 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3184 3216 3363 3395 3594 3626 7101 7133 3184 Size: 32 147 32 199 32 3475 32 3192 Fragments arranged by size: 3475 3192 199 147 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7141 XbaI T'CTAG_A Cuts at: 4477 4477 Size: 7141 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2414 3056 2414 Size: 642 6499 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7141 XmaI C'CCGG_G Cuts at: 1870 2085 4466 1870 Size: 215 2381 4545 Fragments arranged by size: 4545 2381 215 XmnI GAAnn'nnTTC Cuts at: 5270 5270 Size: 7141 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4949 276 Size: 53 83 186 936 3415 2468 Fragments arranged by size: 3415 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI