Restriction map for
pFN21AE2098
(Linear) (Six-base) MAPSORT of: KIEE2098.seq Check: 4231 from: 1 to: 2436
KIEE2098.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 614 960 1454 2306 2436
Size: 614 346 494 852 130
Fragments arranged by size:
852 614 494 346 130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1250 1849 2131 2436
Size: 1250 599 282 305
Fragments arranged by size:
1250 599 305 282
AfeI AGC'GCT
Cuts at: 0 2272 2436
Size: 2272 164
AflII C'TTAA_G
Cuts at: 0 1012 2436
Size: 1012 1424
AflIII A'CryG_T
Cuts at: 0 364 1618 2436
Size: 364 1254 818
Fragments arranged by size:
1254 818 364
AleI CACnn'nnGTG
Cuts at: 0 1815 2436
Size: 1815 621
AlwNI CAG_nnn'CTG
Cuts at: 0 772 1228 1854 2007 2436
Size: 772 456 626 153 429
Fragments arranged by size:
772 626 456 429 153
ApaI G_GGCC'C
Cuts at: 0 118 201 822 1539 1721 2436
Size: 118 83 621 717 182 715
Fragments arranged by size:
717 715 621 182 118 83
AscI GG'CGCG_CC
Cuts at: 0 21 2436
Size: 21 2415
AvaI C'yCGr_G
Cuts at: 0 10 80 111 809 827 959 1836 2436
Size: 10 70 31 698 18 132 877 600
Fragments arranged by size:
877 698 600 132 70 31 18 10
BanI G'GyrC_C
Cuts at: 0 6 90 308 1057 1128 2436
Size: 6 84 218 749 71 1308
Fragments arranged by size:
1308 749 218 84 71 6
BanII G_rGCy'C
Cuts at: 0 106 118 201 548 822 856 1456 1504
Size: 106 12 83 347 274 34 600 48
Cuts at: 1504 1539 1721 1800 2436
Size: 35 182 79 636
Fragments arranged by size:
636 600 347 274 182 106 83 79
48 35 34 12
BbeI G_GCGC'C
Cuts at: 0 10 94 312 2436
Size: 10 84 218 2124
Fragments arranged by size:
2124 218 84 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 340 422 1238 2436
Size: 340 82 816 1198
Fragments arranged by size:
1198 816 340 82
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 622 656 2436
Size: 622 34 1780
Fragments arranged by size:
1780 622 34
BclI T'GATC_A
Cuts at: 0 450 2436
Size: 450 1986
BglI GCCn_nnn'nGGC
Cuts at: 0 904 2241 2436
Size: 904 1337 195
Fragments arranged by size:
1337 904 195
BlpI GC'TnA_GC
Cuts at: 0 542 629 881 1140 1450 2436
Size: 542 87 252 259 310 986
Fragments arranged by size:
986 542 310 259 252 87
Bme1580I G_kGCm'C
Cuts at: 0 118 201 822 1131 1539 1572 1721 2436
Size: 118 83 621 309 408 33 149 715
Fragments arranged by size:
715 621 408 309 149 118 83 33
BmgBI CAC'GTC
Cuts at: 0 77 859 2436
Size: 77 782 1577
Fragments arranged by size:
1577 782 77
BmtI G_CTAG'C
Cuts at: 0 1139 2436
Size: 1139 1297
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 583 1064 1489 1630 1786 2269 2287
Size: 202 381 481 425 141 156 483 18
Cuts at: 2287 2436
Size: 149
Fragments arranged by size:
483 481 425 381 202 156 149 141
18
Bpu10I CC'TnA_GC
Cuts at: 0 1154 2436
Size: 1154 1282
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 577 2436
Size: 262 315 1859
Fragments arranged by size:
1859 315 262
BsaI GGTCTCn'nnnn_
Cuts at: 0 559 2436
Size: 559 1877
BsaAI yAC'GTr
Cuts at: 0 412 1621 2436
Size: 412 1209 815
Fragments arranged by size:
1209 815 412
BsaHI Gr'CG_yC
Cuts at: 0 7 91 309 441 2436
Size: 7 84 218 132 1995
Fragments arranged by size:
1995 218 132 84 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 61 91 2436
Size: 61 30 2345
Fragments arranged by size:
2345 61 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1083 1759 2184 2436
Size: 1083 676 425 252
Fragments arranged by size:
1083 676 425 252
BseYI C'CCAG_C
Cuts at: 0 1032 1333 1799 2058 2273 2436
Size: 1032 301 466 259 215 163
Fragments arranged by size:
1032 466 301 259 215 163
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 568 932 1009 1241 2436
Size: 457 111 364 77 232 1195
Fragments arranged by size:
1195 457 364 232 111 77
BsiHKAI G_wGCw'C
Cuts at: 0 106 979 1200 1456 1638 1822 1991 2436
Size: 106 873 221 256 182 184 169 445
Fragments arranged by size:
873 445 256 221 184 182 169 106
BsmI GAATG_Cn'
Cuts at: 0 1716 2151 2436
Size: 1716 435 285
Fragments arranged by size:
1716 435 285
Bsp1286I G_dGCh'C
Cuts at: 0 106 118 201 548 822 856 979 1131
Size: 106 12 83 347 274 34 123 152
Cuts at: 1131 1200 1456 1504 1539 1572 1638 1721 1800
Size: 69 256 48 35 33 66 83 79
Cuts at: 1800 1822 1991 2436
Size: 22 169 445
Fragments arranged by size:
445 347 274 256 169 152 123 106
83 83 79 69 66 48 35 34
33 22 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 614 960 1454 2306 2380 2436
Size: 614 346 494 852 74 56
Fragments arranged by size:
852 614 494 346 74 56
BsrBI CCG'CTC
Cuts at: 0 188 2436
Size: 188 2248
BsrFI r'CCGG_y
Cuts at: 0 911 2436
Size: 911 1525
BssHII G'CGCG_C
Cuts at: 0 21 2436
Size: 21 2415
BstAPI GCAn_nnn'nTGC
Cuts at: 0 651 2436
Size: 651 1785
BstEII G'GTnAC_C
Cuts at: 0 1067 2293 2436
Size: 1067 1226 143
Fragments arranged by size:
1226 1067 143
BstXI CCAn_nnnn'nTGG
Cuts at: 0 384 1341 1894 2265 2436
Size: 384 957 553 371 171
Fragments arranged by size:
957 553 384 371 171
BstYI r'GATC_y
Cuts at: 0 1329 2436
Size: 1329 1107
BtgI C'CryG_G
Cuts at: 0 65 669 1789 2280 2436
Size: 65 604 1120 491 156
Fragments arranged by size:
1120 604 491 156 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 900 1754 2436
Size: 900 854 682
Fragments arranged by size:
900 854 682
BtsI GCAGTG_nn'
Cuts at: 0 1597 1636 1810 2436
Size: 1597 39 174 626
Fragments arranged by size:
1597 626 174 39
EaeI y'GGCC_r
Cuts at: 0 572 905 2436
Size: 572 333 1531
Fragments arranged by size:
1531 572 333
EarI CTCTTCn'nnn_
Cuts at: 0 94 473 1107 1277 2311 2436
Size: 94 379 634 170 1034 125
Fragments arranged by size:
1034 634 379 170 125 94
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 589 1016 1504 2436
Size: 589 427 488 932
Fragments arranged by size:
932 589 488 427
EcoICRI GAG'CTC
Cuts at: 0 104 1454 2436
Size: 104 1350 982
Fragments arranged by size:
1350 982 104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 583 1064 1250 1489 1630 1786 1849
Size: 202 381 481 186 239 141 156 63
Cuts at: 1849 2131 2269 2287 2436
Size: 282 138 18 149
Fragments arranged by size:
481 381 282 239 202 186 156 149
141 138 63 18
EcoO109I rG'GnC_Cy
Cuts at: 0 115 198 769 819 839 1213 1536 1653
Size: 115 83 571 50 20 374 323 117
Cuts at: 1653 1717 1718 2115 2436
Size: 64 1 397 321
Fragments arranged by size:
571 397 374 323 321 117 115 83
64 50 20 1
HaeII r_GCGC'y
Cuts at: 0 10 94 167 312 2058 2274 2436
Size: 10 84 73 145 1746 216 162
Fragments arranged by size:
1746 216 162 145 84 73 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 61 93 823 855 1547 1576 1579 1608
Size: 61 32 730 32 692 29 3 29
Cuts at: 1608 2161 2173 2176 2193 2205 2208 2436
Size: 553 12 3 17 12 3 228
Fragments arranged by size:
730 692 553 228 61 32 32 29
29 17 12 12 3 3 3
HincII GTy'rAC
Cuts at: 0 1671 2436
Size: 1671 765
HindIII A'AGCT_T
Cuts at: 0 1361 2436
Size: 1361 1075
KasI G'GCGC_C
Cuts at: 0 6 90 308 2436
Size: 6 84 218 2128
Fragments arranged by size:
2128 218 84 6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 516 1754 2246 2436
Size: 516 1238 492 190
Fragments arranged by size:
1238 516 492 190
MscI TGG'CCA
Cuts at: 0 574 907 2436
Size: 574 333 1529
Fragments arranged by size:
1529 574 333
MslI CAynn'nnrTG
Cuts at: 0 424 1580 1815 2436
Size: 424 1156 235 621
Fragments arranged by size:
1156 621 424 235
MspA1I CmG'CkG
Cuts at: 0 456 648 1337 1686 1854 2208 2436
Size: 456 192 689 349 168 354 228
Fragments arranged by size:
689 456 354 349 228 192 168
NaeI GCC'GGC
Cuts at: 0 913 2436
Size: 913 1523
NarI GG'CG_CC
Cuts at: 0 7 91 309 2436
Size: 7 84 218 2127
Fragments arranged by size:
2127 218 84 7
NcoI C'CATG_G
Cuts at: 0 65 669 2436
Size: 65 604 1767
Fragments arranged by size:
1767 604 65
NdeI CA'TA_TG
Cuts at: 0 170 2436
Size: 170 2266
NgoMIV G'CCGG_C
Cuts at: 0 911 2436
Size: 911 1525
NheI G'CTAG_C
Cuts at: 0 1135 2436
Size: 1135 1301
NspI r_CATG'y
Cuts at: 0 368 516 2436
Size: 368 148 1920
Fragments arranged by size:
1920 368 148
PciI A'CATG_T
Cuts at: 0 364 2436
Size: 364 2072
PflMI CCAn_nnn'nTGG
Cuts at: 0 772 2436
Size: 772 1664
PfoI T'CCnGG_A
Cuts at: 0 1607 2436
Size: 1607 829
PmlI CAC'GTG
Cuts at: 0 1621 2436
Size: 1621 815
PpuMI rG'GwC_Cy
Cuts at: 0 839 1653 2436
Size: 839 814 783
Fragments arranged by size:
839 814 783
PshAI GACnn'nnGTC
Cuts at: 0 440 2436
Size: 440 1996
PspOMI G'GGCC_C
Cuts at: 0 114 197 818 1535 1717 2436
Size: 114 83 621 717 182 719
Fragments arranged by size:
719 717 621 182 114 83
PstI C_TGCA'G
Cuts at: 0 521 897 1185 1850 2436
Size: 521 376 288 665 586
Fragments arranged by size:
665 586 521 376 288
PvuII CAG'CTG
Cuts at: 0 648 1337 1854 2208 2436
Size: 648 689 517 354 228
Fragments arranged by size:
689 648 517 354 228
SacI G_AGCT'C
Cuts at: 0 106 1456 2436
Size: 106 1350 980
Fragments arranged by size:
1350 980 106
SanDI GG'GwC_CC
Cuts at: 0 839 2436
Size: 839 1597
SapI GCTCTTCn'nnn_
Cuts at: 0 94 2436
Size: 94 2342
SfcI C'TryA_G
Cuts at: 0 517 893 1181 1846 1999 2436
Size: 517 376 288 665 153 437
Fragments arranged by size:
665 517 437 376 288 153
SfoI GGC'GCC
Cuts at: 0 8 92 310 2436
Size: 8 84 218 2126
Fragments arranged by size:
2126 218 84 8
SmaI CCC'GGG
Cuts at: 0 82 2436
Size: 82 2354
SmlI C'TyrA_G
Cuts at: 0 277 592 1012 2436
Size: 277 315 420 1424
Fragments arranged by size:
1424 420 315 277
StuI AGG'CCT
Cuts at: 0 16 287 342 391 805 1834 2410 2436
Size: 16 271 55 49 414 1029 576 26
Fragments arranged by size:
1029 576 414 271 55 49 26 16
StyI C'CwwG_G
Cuts at: 0 65 669 1391 2162 2436
Size: 65 604 722 771 274
Fragments arranged by size:
771 722 604 274 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1449 1691 2436
Size: 1449 242 745
Fragments arranged by size:
1449 745 242
TatI w'GTAC_w
Cuts at: 0 1922 2436
Size: 1922 514
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1179 1211 1358 1390 1589 1621 2436
Size: 1179 32 147 32 199 32 815
Fragments arranged by size:
1179 815 199 147 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 409 1051 2436
Size: 409 642 1385
Fragments arranged by size:
1385 642 409
XmaI C'CCGG_G
Cuts at: 0 80 2436
Size: 80 2356
Enzymes that do cut:
AarI AcuI AfeI AflII AflIII AleI AlwNI ApaI
AscI AvaI BanI BanII BbeI BbsI BcgI BclI
BglI BlpI Bme1580I BmgBI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstEII
BstXI BstYI BtgI BtgZI BtsI EaeI EarI EciI
EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII HindIII KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NspI PciI PflMI PfoI PmlI PpuMI
PshAI PspOMI PstI PvuII SacI SanDI SapI SfcI
SfoI SmaI SmlI StuI StyI TaqII TatI TstI
XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AgeI AhdI AlfI AloI
ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbvCI
BciVI BfrBI BglII BmrI BplI BsaBI BsaWI BsiEI
BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HpaI
KpnI MfeI MluI NotI NruI NsiI PacI PasI
PmeI PpiI PsiI PspXI PsrI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI
XmnI ZraI