################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BalI BbeI BcgI BciVI BclI BfrI BglII BlnI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BstAPI BstBI BstEII BstPI BstXI Bsu36I ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI KpnI MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NarI NcoI NdeI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SalI SapI ScaI SfiI SfoI SfuI SmlI SnaBI SpeI SphI SspBI SspI SwaI Tru9I Tsp509I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 783 GTATAC GT^[AC][GT]AC AcyI 259 GACGCC G[GA]^CG[CT]C Aor51HI 288 AGCGCT AGC^GCT AspHI 1064 GAGCTC G[AT]GC[AT]^C AspI 151 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 140 TGCGCA TGC^GCA BanI 340 GGTGCC G^G[CT][GA]CC BbsI 973 GTCTTC GAAGAC(2/6) BbvCI 381 CCTCAGC CCTCAGC(-5/-2) BlpI 570 GCTGAGC GC^T[ACGT]AGC Bpu1102I 570 GCTGAGC GC^T[ACGT]AGC BpuAI 973 GTCTTC GAAGAC(2/6) BsaHI 259 GACGCC G[GA]^CG[CT]C BsgI 501 CTGCAC GTGCAG(16/14) BsiHKAI 1064 GAGCTC G[AT]GC[AT]^C BspMI 71 ACCTGC ACCTGC(4/8) BssHII 402 GCGCGC G^CGCGC BssSI 904 CACGAG CACGAG(-5/-1) Bst1107I 783 GTATAC GTA^TAC BstF5I 378 CATCC GGATG(2/0) BstZ17I 783 GTATAC GTA^TAC CelII 570 GCTGAGC GC^T[ACGT]AGC DraIII 217 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 2 TGGCCG [CT]^GGCC[GA] EarI 82 CTCTTC CTCTTC(1/4) Eco47III 288 AGCGCT AGC^GCT EcoT14I 494 CCTTGG C^C[AT][AT]GG FokI 378 CATCC GGATG(9/13) FspI 140 TGCGCA TGC^GCA Hin1I 259 GACGCC G[GA]^CG[CT]C Ksp632I 82 CTCTTC CTCTTC(1/4) KspI 414 CCGCGG CCGC^GG MaeIII 215 GTCAC ^GT[ACGT]AC NheI 132 GCTAGC G^CTAGC PflFI 151 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 694 GAGTC GAGTC(4/5) SacI 1064 GAGCTC GAGCT^C SacII 414 CCGCGG CCGC^GG SbfI 648 CCTGCAGG CCTGCA^GG SexAI 725 ACCAGGT A^CC[AT]GGT SfaNI 377 GCATC GCATC(5/9) SgrAI 261 CGCCGGCG C[GA]^CCGG[CT]G Sse8387I 648 CCTGCAGG CCTGCA^GG StuI 762 AGGCCT AGG^CCT StyI 494 CCTTGG C^C[AT][AT]GG TfiI 1020 GATTC G^A[AT]TC Tsp45I 215 GTCAC ^GT[GC]AC Tth111I 151 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 198 GTAC GT^AC 1081 GTAC BanII 573 GAGCCC G[GA]GC[CT]^C 1064 GAGCTC BbrPI 40 CACGTG CAC^GTG 772 CACGTG BsaAI 40 CACGTG [CT]AC^GT[GA] 772 CACGTG BsrFI 262 GCCGGC [GA]^CCGG[CT] 373 GCCGGC BsrI 1039 ACTGG ACTGG(1/-1) 834 CCAGT Cfr10I 262 GCCGGC [GA]^CCGG[CT] 373 GCCGGC DsaI 356 CCGTGG C^C[GA][CT]GG 414 CCGCGG HinfI 694 GAGTC G^A[ACGT]TC 1020 GATTC NaeI 262 GCCGGC GCC^GGC 373 GCCGGC NgoMIV 262 GCCGGC G^CCGGC 373 GCCGGC PflMI 93 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 834 CCAGTTCCTGG PmaCI 40 CACGTG CAC^GTG 772 CACGTG PmlI 40 CACGTG CAC^GTG 772 CACGTG RsaI 198 GTAC GT^AC 1081 GTAC SmaI 275 CCCGGG CCC^GGG 463 CCCGGG TspRI 807 CACTG CA[GC]TG[ACGT][ACGT]^ 1038 CACTG Van91I 93 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 834 CCAGTTCCTGG XmaCI 275 CCCGGG C^CCGGG 463 CCCGGG XmaI 275 CCCGGG C^CCGGG 463 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AccII AciI AluI AlwI AlwNI AvaI AvaII BamHI BbvI BcnI BfaI BglI BmyI BpmI BsaJI BsiYI BslI BsmFI BsoBI Bsp1286I BssKI BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeI MaeII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIV PstI Sau3AI Sau96I ScrFI SfcI TaqI TseI TthHB8I XcmI XhoII